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All Journal JURNAL TEKNOLOGI LINGKUNGAN BIOTROPIA - The Southeast Asian Journal of Tropical Biology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Biosaintifika: Journal of Biology & Biology Education Jurnal Golden Age Makara Journal of Science
Sri Widawati
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jln. Raya Jakarta-Bogor Km. 46, Cibinong Science Center, Jawa Barat, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Social Sciences Veterinary

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PENGARUH KOMPOS DAN BERBAGAI PUPUK HAYATI TERHADAP PERTUMBUHAN DAN HASIL TEMULAWAK (CURCUMA XANTHORRHIZA) Sugiharto, A.; Widawati, Sri
JURNAL BIOLOGI INDONESIA Vol 3, No 9 (2005): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i9.3280

Abstract

ABSTRACTThe Influence of Compos and Several Biofertilizers on the Growth and the Harvesting ofTemulawak (Curcuma xanthorrhiza). Curcuma xanthorriza is one of the potentialmedicinal plants. Usually this plant is planted only as additional plant among the mainhorticultural plants. This plant is used as medicine plant, and usually grown not in fertile soils.So, this research tried to use the additional fertilizer such as compost and the potentialmicrobes. The aim of the research was to know the influence of the compost and the potentialisolates on the growth of the Curcuma xanthorrhiza. The research had been done at the greenhouse with Randomized Completely Design, by using polybag with 20 cm diameter. Therewas three replications. The results analyzed with ANOVA method, and Duncan Test at 0,05.The used soil was podsolik yellowish red that collected from Darmaga, Bogor. The resultshowed that the use of compost and biofertilizer increased the harvesting of the tubers from15,76% to 52,79%. The using of either BPP isolates or the mycorrhyza, increased the driedweigh of tubers of Curcuma xanthorrhiza.Keywords : Compost, potential microbes, Curcunla xanthorrhiza
PENGARUH INOKULASI BAKTERI TERHADAP PERTUMBUHAN AWAL JARAK PAGAR (JATROPHA CURCAS L.) Widawati, Sri; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3175

Abstract

ABSTRACTBacterial inoculants affect the early growth of Jatropha (Jatropha curcas L). Genera ofAzotobacter, Bacillus, Chromobacterium, Citrobacter, Nitrosomonas, Rhizobium, andSpaerotillus natans were soil bacterial isolates. The soil was collected from numerous placesaround Pontianak, West Kalimantan. Those isolates were used as inoculants, and formulatedto single and mixed bacterial inoculants, then used to stimulate the early growth of jatrophaseedling in 15 weeks at greenhouse condition. Bacterial inoculations caused better growthperformance compared to its control as pure soil garden medium without inoculations, andneither to bare soil dresses with compost. In the presence of inoculants, plant height wasaccelerated quickly while other inoculants affected to stalk diameter development. Daily growthperformance of jatropha peaked in 8 and 11 weeks after inoculation of Citrobacter andNitrosomonas bacterial component were used as single inoculant, respectively. The increasingof shoot biomass accumulation was three times as caused by single inoculants (Bacillus sp),and the highest one up to four times of biomass weight caused by a mixture inoculants asconsortium of Azotobacter, Bacillus, and Nitrosomonas spp. That selective inoculant hasopportunity to be used for jatropha farming, and this basic study is meaningful to jatropacultivation for standing to bio-fuel resources.Keywords: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.Kata kunci: Jatropha curcas L., inoculants, Azotobacter, Bacillus, Chromobacterium,Citrobacter, Nitrosomonas, Rhizobium , Spaerotillus natans.
Role of Indigenous Nitrogen-fixing Bacteria in Promoting Plant Growth on Post Tin Mining Soil Widawati, Sri; Suliasih,
Makara Journal of Science Vol. 23, No. 1
Publisher : UI Scholars Hub

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Abstract

Post tin mining soil is generally marginal with low pH, has poor nutrient content, and is thus unfavorable for plant growth, particularly for Sorghum bicolor, which is a nutrient-demanding plant. Indigenous bacteria are usually used in bioaugmentation to ameliorate environmental degradation due to their ability to adapt well. This research aimed to isolate indigenous nitrogen-fixing bacteria and evaluate its potential for promoting the growth of S. bicolor on post tin mining soil. Nitrogen-fixing bacteria were isolated from post tin mining soil by using specific media and identified by Bergey’s manual. Twenty five isolates were obtained, and eight of them (Azospirillum sp., Azospirillum lipoferum, Azotobacter chroococcum, A. paspalii, and Rhizobium sp.) were identified as nitrogen-fixing bacteria. A greenhouse experiment was conducted using factorial completely randomized design with three replications. The first factors were fertilizers, i.e., NPK; A. lipoferum CBT4 + NPK; A. lipoferum CBT4; and without fertilizer (control). The second factors were soil types, i.e., A (fertile soil from Cibinong), B (soil from Bangka Botanical Garden), C (soil from post tin mines two years after mining), and D (soil from active tin mining). Result showed that Azospirillum lipoferum CBT4 isolated from C (soil from post tin mines two years after mining) exhibited the highest IAA, Ca-P solubilizing ability, and PME-ase activity. This species survived up to a population of 107 CFU/gram soil in the three types of post tin mining soils and could be a potential plant-growth promoting rhizobacteria (PGPR) species for effectively improving the growth of S. bicolor plant on post tin-mining soil.
The application of Rigidoporus sp J12 and Stenotrophomonas maltophilia BM in the degradation of batik waste Subowo, Yohanes; Suliasih, Suliasih; Widawati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 15, No 3 (2023): December 2023
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i3.41987

Abstract

The batik industry in Indonesia produces batik waste which pollutes the environment. This waste can be degraded using laccase-producing microorganisms. The microorganisms used in the research were the fungus Rigidoporus sp J12 and the bacteria Stenotrophomonas maltophilia BM. This research aims to determine the ability of Rigidoporus sp J12 and Stenotrophomonas maltophilia BM and their consortium in producing laccase, observing their ability to degrade Poly R-478 which is an indicator of phenoloxidase activity and batik waste. Microorganisms are grown in growth media and then placed in media containing Poly R-478 or batik waste. Inducers are added to increase laccase activity. The inducers used were 15 g/L sucrose, 200 µM CuSO4 and 40 mM veratryl alcohol. The results showed that Rigidoporus sp J12 and Stenotrophomonas maltophilia BM produced laccase in PDB and NA media. The highest laccase activity was found in the enzyme produced by Rigidoporus sp J12 in PDB media at a temperature of 40°C, media pH 6.0 and the addition of sucrose. Rigidoporus sp J12 degraded batik waste by 39.38% and increased by 2.12 times after adding sucrose and incubation for 15 days. These bacteria and fungi can be used to degrade batik waste in order to prevent environmental pollution. Using the fungus Rigidoporus sp J12 purely is more profitable than using it with S. maltophilia BM bacteria.
PENGARUH METODE BERCERITA MEDIA BIGBOOK TERHADAP KEMAMPUAN MEMBACA AWAL 2 SUKU KATA ANAK USIA 5-6 TAHUN Dinda Ayu Kartika; Simatupang, Nurhenti Dorlina; Reza, Muhammad; Widawati, Sri
Jurnal Golden Age Vol. 9 No. 1 (2025): Jurnal Golden Age
Publisher : Universitas Hamzanwadi

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penerapan metode bercerita menggunakan media big book terhadap kemampuan membaca awal dua suku kata anak usia 5-6 tahun di TK Pertiwi V Sugihwaras Prambon Kabupaten Nganjuk. Metode penelitian yang digunakan adalah kuantitatif dengan pendekatan Quasi Experimental Design dan rancangan one group pretest-posttest design. Sampel penelitian terdiri dari 20 anak kelompok B. Pengumpulan data dilakukan melalui pretest untuk mengukur kemampuan awal membaca dan posttest setelah pemberian perlakuan. Data dianalisis menggunakan uji Wilcoxon. Hasil penelitian menunjukkan bahwa terdapat peningkatan yang signifikan pada kemampuan membaca anak setelah diberikan perlakuan metode bercerita dengan media big book. Nilai signifikansi asimtotik (2-tailed) yang diperoleh adalah 0,003, yang lebih kecil dari tingkat signifikansi 0,05, sehingga hipotesis alternatif (Ha) diterima. Temuan ini mengindikasikan bahwa metode bercerita dengan media big book memiliki pengaruh positif dalam meningkatkan kemampuan membaca dua suku kata pada anak usia dini.
POTENCY OF RHIZOSPHERE BACTERIA TO PROMOTE RICE GROWTH UNDER SALINE CONDITION Widawati, Sri; Sudiana, I Made
BIOTROPIA Vol. 23 No. 2 (2016): BIOTROPIA Vol. 23 No. 2 December 2016
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.765 KB) | DOI: 10.11598/btb.2016.23.2.511

Abstract

Saline soil is a common problem in coastal paddy field, especially in Indonesia. Salinity affects rice growth and the activities of soil functional microbes, including functional bacteria, which play roles in plant growth. Some of these microbes are associated with rice plants and are able to survive under saline condition. The presence of functional microbes is also important to improve soil quality. Nitrogen and phosphate are essential soil nutrients and is available in soil due to the activities of nitrogen-fixing bacteria and free-living plant-associated bacteria.  The objective of the present study was to obtain nitrogen-fixing, phosphate solubilizing and Indole Acetic Acid  (IAA)-producing bacteria that are able to survive and promote the growth of rice under saline conditions. From rice and peanut rhizosphere, Ca-phosphate (Ca-P) solubilizing and nitrogen-fixing bacteria were isolated separately using specific media. Then, the Ca-P solubilizing ability, phosphomonoesterase activity and IAA-producing ability were quantitatively examined. Based on the abilities, 20 strains were selected and identified as Burkholderia cepacia-complex, Burkholderia anthina, Burkholderia cenocepacia, Bacillus cereus-complex (three strains), Achromobacter spanius, Azospirillum sp. (four strains), Azotobacter sp. (three strains), Rhizobium leguminosarum, Rhizobium sp. (two strains), and Pseudomonas sp. (three strains). The inoculation of several single strains or the mixture of the selected strains promoted the growth of rice under saline conditions. These inoculants could be potential as biofertilizer in saline paddy fields.
PHYTASE PRODUCTION BY Enterobacter cloacae Suliasih, Suliasih; Widawati, Sri
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.0.0.0.1233

Abstract

This study aims to isolate phytase-producing bacteria (PPB), a type of plant growth-promoting rhizobacteria (PGPR), from the Vigna sinensis rhizosphere and to optimize their physicochemical conditions. Phytase is an enzyme that hydrolyzes the phosphoester bonds in organic phosphorus (phytic acid) to release ester phosphate and inorganic phosphate, the bioavailable forms of phosphorus. To test their ability to hydrolyze organic phosphates (calcium phytate), phytase activity was screened using solid and liquid phytase screening medium (PSM). A total of 13 isolates tested positive for phytase production, indicated by clear hydrolysis zones surrounding the colonies. Enterobacter cloacae strain B1 exhibited the highest hydrolysis efficiency (3.43) on solid medium. The phytase produced by E. cloacae strain B1 in liquid PSM reached 0.92 U/mL after 48 hours of incubation. This strain produced optimal phytase levels in the presence of lactose and monoammonium phosphate (NH₄H₂PO₄) as carbon and nitrogen sources, respectively, at 30 °C and pH 5.0. The PPB obtained in this study are recommended for further research regarding their potential use as biological fertilizers for plants.
CHARACTERIZATION OF RHIZOBACTERIA ISOLATES FROM SOIL AND NODULES Widawati, Sri; Suliasih, Suliasih; Susilowati, Dwi Ningsih; Muramatsu, Yuki; Sudiana, I Made
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (290.948 KB) | DOI: 10.11598/btb.0.0.0.1241

Abstract

The plant growth promoting rhizobacteria (PGPR) is a group of bacteria capable of colonizing plants roots, thereby developing a system and improving plants growth and yield. The objectives of the study is to characterize the PGPR activities of several bacterial isolates {in-vitro screening), to examine their activities in stimulating soybean growth (in-vivo screening), and to identify the bacterial species. These were isolated from nodules and soil samples collected from Mount Pancar in Bogor, West Java Province as well as from Bangkirai Hill and Wain River in East Kalimantan, Indonesia. The in-vitro PGPR activity characterization includes the N-fixing ability, ACC-deaminase, indole acetic acid (IAA) production, cellulolytic activity, P-solubilization, Phosphomonoesterase (PME-ase), and nifH-gene detection. The in-vivo PGPR activity with the greenhouse assay was conducted on soybean plant {Glycine max L.). All bacterial isolates were identified using molecular methods based on nucleotide sequence generated from 16S rRNA gene. Three isolates of soil and nodule bacteria with 7 characteristics of PGPR (N2 fixation, ACC-deaminase, cellulolytic activity, IAA production, solubilization index, P available, and PMEase activity) were successfully identified. These isolates were B045 {Klebsiella variicola InaCC B827), B116 (Klebsiella sp. InaCC B833), and B210 {Mangrovibacterplantisponsorlaa.CC B841). The greenhouse assay showed that the plant height, plant dry weight and number of flowers in soybean seedlings significantly increased with Bradyrhispbium sp. strain 4167, then with Klebsiella sp. InaCC B833 and Mangrovibacterplantisponsor InaCC B841. These bacterial isolates which were characterized and screened in-vitro for PGPR potentials and their representative isolates which were identified by 16S rRNA sequence analysis are key factors for selecting PGPR isolates to be commercialized later as bio-stimulant.
Co-Authors Arwan Sugiharto Dinda Ayu Kartika DWI NINGSIH SUSILOWATI I Made Sudiana Maman Rahmansyah Muhammad Reza Muramatsu, Yuki Nurhenti Dorlina Simatupang Subowo, Yohanes Sugiharto, A. Sugiharto, A. Suliasih Suliasih Suliasih, Syaifudin Syaifudin, Syaifudin YB. Subowo Yohanes Bernard Subowo, Yohanes Bernard