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All Journal Folia Medica Indonesiana Jurnal Kedokteran Hewan
Ngakan Made Rai Widjaja
Fakultas Kedokteran Hewan Universitas Airlangga, Surabaya
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary Other

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The Effect of Methylmercury Exposure on Astrocyte of Cerebellar Cortex of White Rats (Rattus novergicus) Sugianto, Paulus; Pardede, Sabrina Melisa; Widjaja, Ngakan Made Rai; Widjiati, Widjiati
Folia Medica Indonesiana Vol. 55 No. 2 (2019): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.194 KB) | DOI: 10.20473/fmi.v55i2.24598

Abstract

The aim of this research was to investigate the effect of different dose methylmercury (II) chloride on astrocyte in cerebellar cortex of white rat (Rattus norvegicus) exposed. This study used randomized control design using 15 adult female Wistar rats weight 180-200 g of body weight. Before treatment the white rats was adapted in a week, then randomly divided into 3 groups each consist of 5 rats. P0 as control were given 0.5 ml aquades, P1 and P2 were given 0.1 and 0.2 mg/kg/day respectively. All groups were given treatment per oral in 30 days with sonde. The data was analyzed by ANOVA, Duncan's multiple range (Duncan's Multiple Range Test). White rats exposed by methylmercury (II) chloride, had a significant differences in the percentage of necrotic astrocyte (p<0.05). Methylmercury chloride exposure increases the number of necrotic astrocytes on white rat.
IDENTIFIKASI GROWTH DIFFERENTIATION FACTOR-9 (GDF-9) DARI MATURASI IN VITRO OOSIT SAPI DENGAN TEKNIK IMUNOSITOKIMIA w, Widjiati; Wulansari, Desi; w, Wurlina; Widjaja, Ngakan Made Rai
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i2.356

Abstract

Penelitian ini bertujuan mengindentifikasi GDF-9 di dalam oosit yang berasal dari preantral folikel sapi yang dimatangkan secara in vitro. Penelitian ini menggunakan metode imunositokimia ikatan antara molekul avidin dan biotin yang terkandung dalam antibodi sekunder bentuk kompleks avidin-biotin. Ovarium sapi diambil dari rumah potong hewan (RPH) Pegirian Surabaya. Penelitian ini menggunakan 105 oosit matang yang berasal dari 62 ovarium. Hasil imunositokimia menunjukkan bahwa 77,14% GDF-9 teridentifikasi.
Co-Authors Desi Wulansari Pardede, Sabrina Melisa Pardede, Sabrina Melisa Paulus Sugianto, Paulus Widjiati w Widjiati Widjiati, Widjiati Wurlina, W