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All Journal Nexus Biomedika Journal Orthopaedi and Traumatology Surabaya (JOINTS)
Ibnu Yudistiro
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology

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Analisis Molekuler Regio Core Promoter dan Precore/CoreIsolat Virus Hepatitis B 09IDSKAB-3 Yudistiro, Ibnu; Prasetyo, Afiono Agung; Sari, Yulia
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: HBV replicates its DNA genome through reverse transcription from RNA intermediate. It is vulnerable to a high number of mutations during such reverse transcription which are frequently found in core promoter and precore/core regions. This study was aimed to identify genetic variation of HBV core promoter and  precore/core regions of 09IDSKAB-3 isolate. Methods: DNA extraction was performed on 09IDSKAB-3 blood sample that was taken from Man Sex with Man Community. Core promoter and precore/core regions were determined by PCR using KL-28 and KL-6 primers and direct sequencing of the corresponding region. Molecular analysis was performed using MEGA 4.0. Results: Based on BLAST result, 09IDSKAB-3 HBV isolate had the highest similarity to isolate AP011085 from DKI Jakarta. Genetic variations A1726C in core promoter, and T1860C, C1877T, G1957C in precore/core region were found in 09IDSKAB-3 isolate. Conclusions: 09IDSKAB-3 HBV isolate was classified into genotype B and subgenotype B3 based on core promoter and precore/core region. The genetic variations found in this isolate may have influence to the replication efficiency and HBeAg/HBcAg production, therefore need further study. Keywords: hepatitis B virus, molecular analysis, core promoter region, precore/core region
Analisis Molekuler Regio Core Promoter dan Precore/CoreIsolat Virus Hepatitis B 09IDSKAB-3 Ibnu Yudistiro; Afiono Agung Prasetyo; Yulia Sari
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: HBV replicates its DNA genome through reverse transcription from RNA intermediate. It is vulnerable to a high number of mutations during such reverse transcription which are frequently found in core promoter and precore/core regions. This study was aimed to identify genetic variation of HBV core promoter and precore/core regions of 09IDSKAB-3 isolate. Methods: DNA extraction was performed on 09IDSKAB-3 blood sample that was taken from Man Sex with Man Community. Core promoter and precore/core regions were determined by PCR using KL-28 and KL-6 primers and direct sequencing of the corresponding region. Molecular analysis was performed using MEGA 4.0. Results: Based on BLAST result, 09IDSKAB-3 HBV isolate had the highest similarity to isolate AP011085 from DKI Jakarta. Genetic variations A1726C in core promoter, and T1860C, C1877T, G1957C in precore/core region were found in 09IDSKAB-3 isolate. Conclusions: 09IDSKAB-3 HBV isolate was classified into genotype B and subgenotype B3 based on core promoter and precore/core region. The genetic variations found in this isolate may have influence to the replication efficiency and HBeAg/HBcAg production, therefore need further study. Keywords: hepatitis B virus, molecular analysis, core promoter region, precore/core region
Correlative Analysis of Radiological Measurements and 1-year SRS-22r in Early and Middle Adolescents with Adolescent Idiopathic Scoliosis: A Retrospective Study Ermawan, Rieva; Jiwandono, Bayu Sakti; Yudistiro, Ibnu; Abdulhamid, Muhammad; Corrigan, Hubertus; Renata, Felicia
(JOINTS) Journal Orthopaedi and Traumatology Surabaya Vol. 14 No. 1 (2025): April 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/joints.v14i1.2025.26-36

Abstract

Background: Patient-reported outcomes are increasingly valuable for assessing the effectiveness of clinical interventions from the patient's perspective. This study aims to investigate the differences in the correlation between radiological measurements and 1-year postoperative SRS-22r domain scores in early (EA) and middle adolescence (MA) patients with adolescent idiopathic scoliosis (AIS), as well as analyze the correlation of these outcomes across different Lenke curve types. Methods: We reviewed records of 87 female AIS patients who underwent scoliosis corrective surgery, collecting data on age, body mass index, preoperative and postoperative radiographic measurements, and 1-year postoperative quality of life assessments, including function, pain, self-image, and satisfaction scores from the SRS-22r questionnaire. Patients were categorized into subgroups based on age and the number of curves. The Spearman correlation test evaluated correlations between radiographic measures and SRS-22r domain scores across these subgroups. Results: Significant correlations were found between self-image and preoperative main apical vertebral translation (AVT), Cobb angle, and trunk shift, with stronger associations in EA than MA. Pain had a weak inverse correlation with the postoperative main Cobb angle, notably in the one-curve group and more strongly in EA. Satisfaction scores decreased with age and were lower in the two-curve group and EA. Conclusions: Cobb angle correction in AIS patients strongly correlates to postoperative self-image, particularly in Lenke type 2, 3, and 6. In Lenke type 1 and type 5, greater correction of the Cobb angle is associated with increased postoperative pain.
Co-Authors Afiono Agung Prasetyo Afiono Agung Prasetyo Corrigan, Hubertus Ermawan, Rieva Jiwandono, Bayu Sakti Muhammad Abdulhamid Renata, Felicia Tamaulina Br Sembiring Yulia Sari