Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

0.23

P-Index

This Author published in this journals

All Journal Microbiology Indonesia Chimica et Natura Acta Jurnal Kimia Terapan Indonesia Nusantara Science and Technology Proceedings

PRASETYAWAN YUNIANTO

Unknown Affiliation

Author-ID : 425540

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology

Published : 5 Documents Claim Missing Document

Claim Missing Document

Articles

Isolasi, Validasi Metode dan Optimasi Awal Proses Ekstraksi Senyawa Penanda Eurycomanon dari Akar Tanaman Pasak Bumi (Eurycoma longifolia) Yunianto, Prasetyawan; Nurhadi, Nurhadi; Supriyono, Agus
Chimica et Natura Acta Vol 5, No 2 (2017)
Publisher : Departemen Kimia

Pasak Bumi (Eurycoma longifolia) secara etnofarmakologi digunakan sebagai obat herbal tradisional yang digunakan untuk afrodisiak dan vitalitas. Eurycomanon digunakan sebagai senyawa penanda dari ekstrak akar pasak bumi, namun kadar yang diperbolehkan dalam ekstrak pasak bumi sesuai standar Malaysia pada kisaran 0,8â1,5%, karena sifatnya yang toksik. Senyawa eurycomanon sebagai senyawa penanda dariÂ pasak bumi masih jarang dan harganya cukup mahal. Oleh karena itu, sangat diperlukan isolasi eurycomanon dan validasi metode analisanya serta proses ekstraksinya. Proses isolasi diawali dengan ekstraksi metode maserasi menggunakan pelarut metanol terhadap akar pasak bumi yang sudah dibuat serbuk. Hasil ekstraksi dipekatkan dan difraksinasi dengan pelarut air dan metanol secara bertahap dari 0% - 100% metanol mengunakan kolom resin stiren-divinilbenzen. Fraksi target dicek menggunakan UPLC-MS dan dilakukan isolasi lebih lanjut dengan HPLC semi preparatif menggunakan kolom RP C-18. Hasil isolasi dicek kemurnian dan spectrum UV-nya dengan HPLC analitis dengan detektor PDA (photo diode array) dan dilakukan elusidasi stuktur dengan NMR. Hasil isolat yang terkonfirmasi sebagai eurycomanon dilakukan validasi metode analisa dan digunakan untuk menganalisa hasil optimasi awal proses ekstraksi. Fraksi target diperoleh dari fraksi 25% metanol dan disolasi lebih lanjut menggunakan HPLC semi preparatif. Isolat yang diperoleh telah dikonfirmasi dengan elusidasi stuktur NMR sebagai senyawa eurycomanon dari akar pasak bumi dengan kemurnian 96%. Metode analisa diperoleh dengan menggunakan HPLC secara gradien elusi (asetonitril:air) padaÂ detektor UV 254 nm dengan kolom RP C-18, 150 x 46 mm, 5 Âµm. Kajian awal proses menunjukkan bahwa ekstraksi terbaik untuk akar pasak bumi dengan kadar etanol 30% pada suhu 50Â°C. Kajian ini dapat digunakan sebagai pembanding proses esktraksi akar pasak bumi yang berbeda dengan Buku PedomanÂ Teknologi Formulasi Sediaan berbasis Ekstrak dari BPOM tahun 2013 Volume 2, yaitu menggunakan sistem maserasi dengan pelarut etanol 96% dengan perbandingan 1:10.

Validasi Metode RP-HPLC untuk Penentuan Kadar Andrografolid Sebagai Senyawa Penanda pada Campuran Esktrak Purwati, Anisyah Is; Yunianto, Prasetyawan; Supriyono, Agus
Chimica et Natura Acta Vol 5, No 3 (2017)
Publisher : Departemen Kimia

Andrografolid merupakan senyawa bioaktif dari tanaman sambiloto (Andrographis paniculata) yang berpotensi sebagai bahan obat. Kadar andrografolid dapat ditentukan menggunakan RP-HPLC. Penelitian ini bertujuan mengembangkan dan memvalidasi metode RP-HPLC untuk menentukan kadar andrografolid sebagai senyawa penanda untuk standarisasi formula obat herbal terstandar (OHT) dari campuran ekstrak tanaman sambiloto dan binahong (Andredera cordifolia). Metode RP-HPLC dikembangkan menggunakan kolom Sunfire C18 (150 Ã 4,6 mm, 5Î¼m) dengan fase gerak asetonitril dan campuran 0,1% asam trifloroasetat dan metanol (90:10) secara elusi gradien serta laju alir fase gerak 1 mL/menit. Deteksi menggunakan detektor PDA pada panjang gelombang 230 nm. Andrografolid dielusi pada waktu retensi 9,77 dengan resolusi puncak 2,14. Respon dari standar andrografolid linear pada rentang konsentrasi 0,031-0,5 mg/mL dengan r2=0,9999. Akurasi dari metode ini, yaitu 94,46% dengan nilai RSD 1,73%. Presisi, limit deteksi (LOD), dan limit kuantisasi (LOQ) yang diperoleh masing-masing, yaitu 3,46%, 0,028 mg/mL, dan 0,096 mg/mL. Metode yang divalidasi ini dapat digunakan sebagai referensi untuk penentuan kadar andrografolid dalam standarisasi obat herbal terstandar dari campuran ekstrak tanaman.

Isolation and Identification of Endophytic Fungi from Srikaya Plants (Annona squamosa) Having Potential Secondary Metabolites as Anti-Breast Cancer Activity PRASETYAWAN YUNIANTO; SYOFI ROSMALAWATI; INDRA RACHMAWATI; WAHYUDI PRIYONO SUWARSO; WAHONO SUMARYONO
Microbiology Indonesia Vol. 6 No. 1 (2012): March 2012
Publisher : Indonesian Society for microbiology

Annonaceous acetogenin was extracted from Annona squamosa (Srikaya) seeds. It has cytotoxic activity against cancer cells and lower toxicity compared to other cancer drugs. Endophyte from Annonaceae is expected to have similar extracted metabolites to the host, thus increasing the economic value. This research is a preliminary study to obtain active compounds with potential as anti-cancer agents from endophytic fungi of Srikaya plants. Four endophytic fungal strains were isolated from Srikaya plants (Annona squamosa) and identified based on 28S rDNA sequence. The isolates are SKY II.3.1, SKY I.1.2, SKY II.3.2, and SKY III.3.1, and have similarity with Fusarium sp Vega760, Fusarium sp NRRL 22354 NRRL223, Nectria rigidiuscula, and Fusarium sp BOL35, respectively. The identified isolates were fermented in liquid media for three weeks. The liquid and mycelium were extracted using ethyl acetate. Whole extract of each fermented isolate was partitioned and evaporated to obtain ethyl acetate extract. Cytotoxicity assay of ethyl acetate extract was carried out at level 100 ppm by Methyl Thyazole Tetrazolium (MTT) viability test towards MCF-7 (breast cancer cell). The result indicated that each ethyl acetate extract could inhibit the viability of cell MCF-7 with 11.34, 99.78, 91.48, and 96.84%, for SKY II.3.1, SKY I.1.2, SKY II.3.2, and SKY III.3.1, respectively. Based on the results of cytotoxicity assay on MCF-7 breast cancer cells, endophytic fungi isolates SKY I.1.2, SKY II.3.2, and SKY III.3.1 are potential as sources of anti-breast cancer compounds.

PENGUJIAN STABILITAS SEDIAAN LUKA BAKAR BERBAHAN BAKU AKTIF KITOSAN/EKSTRAK PEGAGAN(CENTELLA ASIATICA) Eriawan Rismana; Idah Rosidah; Olivia Bunga; Prasetyawan Yunianto; Erna Erna
Jurnal Kimia Terapan Indonesia Vol 17, No 1 (2015)
Publisher : Research Center for Chemistry - LIPI

The evaluation of wound-healing formula stability with active substance chitosan/Centella asiatica extract has been examined. The stability test was evaluated including physical, chemical and microbiology properties. The stability of physical property was observed by organoleptic parameters such as shape of gel, color, odor, homogeneity and weight of samples, while the stability of chemical property was studied by determination of pH and HPLC chromatogram profile. Meanwhile the stability of microbiology property was determined by contents by microbe and fungi contaminant. The results of evaluation showed that the wound-healing formula had a good stability for all properties after storage at room temperature for 24 weeks and 40 oC/75 % RH for 12 weeks.Keywords: Wound-healing formula, chitosan, Centella asiatica extract, evaluation, stability

The Activity of Extract from Rhizophora mucronata Endophytic Fungi Solid Fermentation Against Staphylococcus aureus and Enterobacter aerogenes Wafa, Nurul; Yunianto, Prasetyawan; Widyaningsih, Tri Dewanti
Nusantara Science and Technology Proceedings Multi-Conference Proceeding Series F
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4610

Endophytic fungi, such as Rhizophora mucronata, can produce bioactive compounds from secondary metabolites that have the potential to become alternative antibiotics. This study aims to determine the activity of solid fermentation extract of mangrove endophytic fungi Rhizopora mucronata on the inhibition of bacteria Staphylococcus aureus and Enterobacter aerogenes. The extracts were fermented using rice media for 21 days and tested for antibacterial activity using disc diffusion method. Two of the five fermented extracts inhibited the growth of Staphylococcus aureus and Enterobacter aerogenes bacteria, namely codes ARM1M1 and DRM3M3. KLT-Bioautography was performed on isolate ARM1M1 to identify compounds that have antibacterial activity. Fermentation time variations were conducted at 0, 4, 8, 11, 14, 16, 18, 20, and 21 days. The disc diffusion method antibacterial test was carried out again to find out on which day of fermentation the extract actively inhibited bacterial activity and HPLC analysis was carried out to determine the retention time of the compound. As a result, Aspergillus sp. from Rhizophora mucronata code ARM1M1 showed the best inhibition against Staphylococcus aureus and Enterobacter aerogenes bacteria on day 20 of fermentation, with a retention time of 35 minutes, and an Rf value of 0.4.

Co-Authors A.A. Ketut Agung Cahyawan W Agus Supriyono Eriawan Rismana Erna Erna Idah Rosidah INDRA RACHMAWATI Olivia Bunga Purwati, Anisyah Is SYOFI ROSMALAWATI Tri Dewanti Widyaningsih Wafa, Nurul Wahono Sumaryono Wahyudi Priyono Suwarso

Title

Found 5 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 5 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 5 Documents
Search