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Journal : agriTECH

Mikroemulsi Minyak dalam Air (O/W) Sebagai Pembawa Α-Tokoferol untuk Menghambat Sunlight Flavor pada Susu Full Cream Akibat Fotooksidasi Sih Yuwanti; Sri Raharjo; Pudji Hastuti; Supriyadi Supriyadi
agriTECH Vol 32, No 2 (2012)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.485 KB) | DOI: 10.22146/agritech.9629

Abstract

The objectives of this research were to determine the highest level of α -tocopherol could be incorporated into O/W microemulsion and to evaluate the effectiveness of O/W microemulsion containing á-tocopherol to inhibit sunlight fl avor formation in photo-oxidized full cream milk. á-Tocopherol (5.000 – 10.000 ppm) was incorporated into O/W microemulsion. The highest level of á-tocopherol in the O/W microemulsion was determined based on its stability after heating at 105 oC for up to 5 hours in an oven. Full cream milk samples with or without the addition of 2 % O/W microemulsion containing á-tocopherol were exposed to fl uorescent light at 2000 Lux in a refrigerated display case at 10 oC for up to 8 hours. Another sample of full cream milk was wrapped in aluminum foil to protect from light exposure. The presence of dimethyl disulfi de was determined with headspace-SPME/SIM GCMS, and sensory test for sunlight fl avor was done using triangle test by 18 selected panelists. The result showed that the highest level of á-tocopherol could be incorporated into the O/W microemulsion was 5.000 ppm. The addition of 2 % O/W microemulsion containing á-tocopherol into photo-oxidized full cream milk effectively inhibit the formation of dimethyl disulfi de and sunlight flavor.ABSTRAKTujuan penelitian ini adalah menentukan konsentrasi tertinggi α-tokoferol yang dapat ditambahkan ke mikroemulsi O/W yang stabil dan mengevaluasi kemampuan α tokoferol dalam mikroemulsi O/W untuk menghambat sunlight flavor pada susu full cream akibat fotooksidasi. Konsentrasi α -tokoferol yang ditambahkan ke dalam mikroemulsi O/W adalah 5.000 – 10.000 ppm. Konsentrasi α-tokoferol tertinggi yang dapat ditambahkan ke dalam mikroemulsi O/W ditentukan dengan uji stabilitas terhadap panas dengan pengovenan 105 oC selama 5 jam. Susu full cream tanpa atau ditambah 2 % mikroemulsi O/W yang mengandung α -tokoferol dipapar cahaya fl uorosens 2000 Lux dalam lemari pajang pada 10 oC selama 8 jam. Sampel susu full cream yang lain dibungkus dengan alumunium foil untuk melindungi dari cahaya. Keberadaan dimetil disulfi da ditentukan dengan metode headspace-SPME/SIM GCMS, dan sunlight flavor diuji secara sensoris dengan metode triangle test menggunakan 18 panelis terseleksi. Hasil penelitian menunjukkan bahwa konsentrasi α-tokoferol tertinggi yang dapat ditambahkan ke dalam mikroemulsi O/W adalah 5.000 ppm. Penambahan 2 % mikroemulsi O/W yang mengandung α -tokoferol dapat menghambat pembentukan dimetil disulfida dan sunlight flavor pada susu full cream yang terpapar cahaya.
Formulasi Mikroemulsi Minyak dalam Air (O/W) yang Stabil Menggunakan Kombinasi Tiga Surfaktan Non Ionik dengan Nilai Hlb Rendah, Tinggi dan Sedang Sih Yuwanti; Sri Raharjo; Pudji Hastuti; Supriyadi Supriyadi
agriTECH Vol 31, No 1 (2011)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (479.536 KB) | DOI: 10.22146/agritech.9722

Abstract

The aim of this research was to determine the proportion of oil, surfactant and water which could produce a stable O/W microemulsion using combination of three nonionic surfactants with low, high and medium HLB values; and to determine the role of surfactant with a medium HLB value in O/W microemulsion formulation. The first group of microemulsions were prepared using combination of Tween 80, Span 80 and Span 40 (80 %:10 %:10 %) with dif- ferent proportions of VCO:surfactant (1:3, 1:3.5 dan 1:4).  The second goups of microemulsion were prepared using combination of Tween 80, Span 80 and Span 40 (90 %:5 %:5 %) with different proportions of VCO:surfactant 1:4,1:4.5 dan 1:5.  The stability of microemulsion was determined during storage at room temperature and after being ovened at 105 0C 5 hours and centrifuged at 2300 g 15 minutes. Microemulsion stability was determined by measur- ing absorbance of the microemulsion at 502 nm and then converted to turbidity (%).  In order to determine the role of surfactant with a medium HLB value in the formulation of O/W microemulsion, one set microemulsions were made without surfactant with a medium HLB value, and another set of microemulsions were prepared with different ratios of low and medium HLB surfactant (1:1, 2:1 and 1:2). The most stable microemulsion was achieved when the proportion of VCO:surfactant:water was 4:20:76 and combination of Tween 80:Span 80:Span 40 with the ratio of 90:3.33:6.67. A more stable O/W microemulsion could be obtained when surfactant with a medium HLB value was added to O/W microemulsion formulation. Surfactant with a medium HLB value would link the oil phase and water phase with sur- factant layer, interaction of surfactant-oil and surfactant-water increased. It provided a smooth transition between oil phase and water phase, and the microemulsion became more stable.ABSTRAKTujuan dari penelitian ini adalah untuk menentukan proporsi minyak, surfaktan dan air yang dapat menghasilkan mik- roemulsi minyak dalam air yang stabil menggunakan kombinasi tiga surfaktan non ionik dengan nilai HLB rendah, tinggi dan sedang; dan mengetahui peran surfaktan dengan nilai HLB sedang dalam formulasi mikroemulsi minyak da- lam air. Kelompok mikroemulsi yang pertama disiapkan dengan kombinasi surfaktan Tween 80, Span 80 dan Span 40 (80 %:10 %:10 %) dan variasi proporsi VCO:surfaktan 1:3, 1:3,5 dan 1:4. Kelompok mikroemulsi yang kedua disiap- kan dengan kombinasi surfaktan Tween 80, Span 80 dan Span 40 (90 %:5 %:5 %) dan variasi proporsi VCO:surfaktan1:4, 1:4,5 dan 1:5. Uji stabilitas mikroemulsi dilakukan dengan disimpan pada suhu kamar, dioven 105 0C selama 5 jam dan disentrifuge 2300 g selama 15 menit. Pengamatan stabilitas mikroemulsi dilakukan dengan mengukur absorbansi mikroemulsi pada λ 502 nm yang dikonversi menjadi persen turbiditas. Untuk mengetahui peran surfaktan dengan HLB sedang maka dibuat mikroemulsi tanpa surfaktan HLB sedang, dan juga dibuat mikroemulsi dengan variasi rasio surfaktan  HLB rendah dan sedang yaitu 1:1, 2:1 dan 1:2. Mikroemulsi paling stabil diperoleh dari formula dengan proporsi VCO:surfaktan:air = 4:20:76 dengan kombinasi surfaktan Tween 80:Span 80:Span 40 = 90:3,33:6,67. Pe- nambahan surfaktan HLB sedang pada formulasi mikroemulsi minyak dalam air lebih menstabilkan mikroemulsi yang dihasilkan. Surfaktan HLB sedang menjembatani fase minyak dan air dengan lapisan surfaktan sehingga meningkatkan interaksi surfaktan-air dan surfaktan-minyak, transisi antara fase minyak dan fase air menjadi lebih halus dan mikro- emulsi menjadi lebih stabil.
Asap Cair Sebagai Pengawet Alami pada Bandeng Presto Sih Yuwanti
agriTECH Vol 25, No 1 (2005)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1500.836 KB) | DOI: 10.22146/agritech.13378

Abstract

"Bandeng presto" is easily spoilage at room temperature. Liquid smoke has properties as antimicrobial and antioxidant, so liquid smoke can act as a natural preservative. The objective of this study was to evaluate the antimicrobial and antioxidant activities of liquid smoke on "bandeng presto". Three concentrations of liquid smoke (2.5%, 5% and 7.5%) together with 25% salt solution was used in immersing bandeng before being cooked under pressure, control was immersed only in salt solution. The result showed that liquid smoke was able to retard the growth of microbial and lipid oxidation on "bandeng presto". The ability of liquid smoke as antimicrobial and antioxidant on "bandeng presto" increased as the increasing of liquid smoke concentration. Liquid smoke of 7.5% prolonged the shelf life of "bandeng presto" from I day to 6 days at room temperature and from 21 days to more than 28 days at cold temperature.