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Kekerasan permukaan semen ionomer kaca konvensional tipe II akibat lama penyimpanan (The surface hardness of type II conventional glass ionomer cement conventional because of the length of storage) Asti Meizarini; Irmawati Irmawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 3 (2005): September 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Type II conventional glass-ionomer cements (GIC) are restorative materials consisting of powder and liquid mixed to produce a plastic mass that subsequently sets to a rigid solid. The early GIC was marketed without the expired date, although this material has been currently marketed with the expired date, how stable the materials are not yet clear. The purpose of this study was to evaluate the powder and the liquid GIC storage on the surface hardness of the samples. Six disc samples (5 mm in diameter and 3 mm thick) were made for each of four groups consisting of powder: aged liquid ages, aged powder: new liquid new, new powder new: new liquid new and aged powder: aged liquid ages respectively. All samples prepared followed the manufacturers’ recommendations and were allowed to set for 15 minutes then they were kept for 24 hours at room temperature before testing. Vickers microhardness was assessed for measuring the surface hardness at the top of the sample surface. The data were statistically analyzed using an ANOVA and LSD (p < 0.05). The results of this study indicated that the highest surface hardness was on the group of new powder: new liquid new = 52.040 VHN. The other groups were nearly similar, for new powder: aged liquid, aged powder: new liquid, aged powder: aged liquid they were 49.558 VHN; 49.123 VHN; 48.938 VHN respectively. Conclusion, powder and liquid of the type II conventional glass ionomer that had been stored for a long time descreased the surface hardness of the glass ionomer cements.

Bahan pemutih gigi dengan sertifikat ADA/ISO (Tooth bleaching material with ADA/ISO certificate) Asti Meizarini; Devi Rianti
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 2 (2005): June 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Bleaching of teeth for cosmetic reasons is a popular aspect of cosmetic dentistry because patients realize the aesthetical benefits of these products. The dentist as a clinician's practitioner must be knowledgeable of the products and their application techniques. Bleaching materials which are safe and effective are the ADA accepted or manufactured by those which have already haved ISO certificate. Dentist must have enough knowledge about in-office bleaching prescribed for home-use bleaching including their contra indication and side effects, to advise the patients and provide effective bleaching services.

Sitotoksisitas bahan restorasi cyanoacrylate pada variasi perbandingan powder dan liquid menggunakan MTT assay (Cytotoxicity of the cyanoacrylate restoration material with variation of powder and liquid ratio by using MTT assay) Asti Meizarini
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 1 (2005): March 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

The requirements for dental material include not toxic, not irritant, no carcinogenic potential, nor cause an allergic response with the use in oral cavity. The cyanoacrylate restoration material has certain substance that can be toxic. Because of the ratio amount of powder and liquid is not known, it can lead the restoration more toxic. The purpose of this study was to know the cytotoxicity of the cyanoacrylate restoration material with different variation of powder and liquid ratio using MTT assay. Six cylinder samples of 5 mm in diameter and 2 mm in thickness were used for each group of 1:1.00; 1:0.75; 1:0.50 powder and liquid ratio of cyanoacrylate restoration materials. Each of samples was immersed in eppendorf micro tubes consisting of media culture. After 24 hour, the immersion of media culture was used to investigate the cytotoxic effect to BHK-21 cell lines by MTT assay method. The density of optic formazan indicated the amount of living cells. All data were statistically analyzed by one-way ANOVA and HSD. The results showed that the percentage of living cells amount of powder and liquid ratio 1:1.00; 1:0.75; 1:0.50 were 98.59%; 95.76%; 94.92% respectively. There was a significant difference between 1:1.00 and 1:0.50 group ratio. The conclusion was that the cytotoxicity between 1:1.00 and 1:0.50 powder and liquid ratio of cyanoacrylate restoration materials in this study decreased.

Kekerasan permukaan email setelah aplikasi gel karbamid peroksida 10% dan pasta buah strawberry Pambudi Santoso T; Devi Rianti; Asti Meizarini
Journal of Dentomaxillofacial Science Vol. 8 No. 2 (2009): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

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he influence of strawberries as bleaching alternative material toward surfacemicrohardness of the teeth is still unknown so that a research should be conducted toquantitatively assess the effects of bleaching with 10% carbamide peroxide andblended strawberry paste on post-extracted incisive teeth by performing superficialmicrohardness analysis. Three groups of sample i.e. group 1 (control): post-extractedincisive teeth soaked in ordinary mineral water, group 2: soaked in carbamideperoxide 10% gel, and group 3: soaked in 100% strawberry paste. The microhardessof each sample group is tested before and after the application of bleaching materials.The result was there was significant difference of strawberry paste compared to CP10% gel and mineral water soaked incisive teeth. The data obtained are tabulated,then analyzed with one way Anova and LSD. It can be concluded that the applicationof carbamide peroxide 10% gel in 6 weeks doesnâ€™t decrease the microhardness ofteeth enamel surface. However, the application of strawberry paste decreases themicrohardness of enamel surface.

Sitotoksisitas pemutih gigi berdasarkan konsentrasi bahan Mardiana A. Adam; Asti Meizarini
Journal of Dentomaxillofacial Science Vol. 9 No. 2 (2010): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v9i2.241

The aim of this study was to evaluate the cytotoxicity of several tooth whitening material,carbamide peroxide 10%, 15%, 20% and hydrogen peroxide 38% toward BHK-21 cellusing MTT assay. Each well of microplates which used for the test were aliquotted BHK21cell suspension, after that the test solution were added to eight well each group,respectively. The result showed that percentages of the living cell at 10% carbamideperoxide group = 86,73%; 15% = 81,22%; 20% = 81,82%; 38% hidrogen peroksida =64,08%, respectively. Anova test and LSD showed no significant difference between 10%,15%, 20% groups, but there are significant toward 38% group and control. The 38%hydrogen peroxide group is expectable to be more cytotoxic than those containing 10%carbamide peroxide, which is equivalent to 3.6% hidrogen peroxide. Conclusion.The10%, 15%, 20 % carbamide peroxide and 38% hydrogen peroxide tooth whiteningagents were not cytotoxic toward BHK-21 cell line using MTT assay within CD

Perubahan warna semen ionomer kaca setelah direndam dalam larutan teh hitam Wahyu Puri Wardhani; Asti Meizarini; Anita Yuliati; Retna Apsari
Journal of Dentomaxillofacial Science Vol. 9 No. 2 (2010): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v9i2.242

Microporosity in glass ionomer cement contributes on its colour stability. In oralcavity GIC interacts with saliva, foods, drinks, and the oral hygiene condition whichcould affect in colour stability. This study aimed to examine the colour change in GICafter immersion in black tea solution. Twenty eight disc specimens (6 mm diameterand 1 mm thickness). The colour measurements were recorded after storage in closedtube for 24 hours. Specimens were randomly devided into 4 groups of 7 specimens.Control specimens were kept in saline solution. The other 3 groups were immersed inblack tea solution for 5 minutes respectively for 5, 7, and 14 times, followed by colourchange measurement. Data were submitted to one-way ANOVA and LSD. There wassignificant colour change in GIC after immersion in black tea. The conclusion islonger GIC immersed in black tea lower the intensity. The lowest intensity changingwas the immersed for 5 minutes, 14 times daily.

Aplikasi pasta stroberi sebagai material bleaching terhadap perubahan warna dan kekerasan permukaan enamel Amelia Hartanto; Devi Rianti; Asti Meizarini
Jurnal Material Kedokteran Gigi Vol 1 No 1 (2012): JMKG Vol 1 No 1 Maret 2012
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

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Utilization of natural products are very popular in the community lately as a natural substance that is considered more secure, inexpensive, and easy to obtain than chemical. one of which is to use strawberries as an alternative bleaching materials. The purpose of this research is to determine the optimal time of strawberry paste application as bleaching material that has not lowered the enamel surface hardness. Research method contain six sample groups of post extracted incisive teeth after discolorized with black tea in 12 days. Each sample was soaked in 100% strawberry paste in 5 minutes per 8 hours. Soakings were done in 2, 3, 4, 5 weeks. Then, color discoloration observed using Vita shade guide and the enamel surface hardness measured using Vickers hardness tester. Enamel surface hardness data tabulated and then analyzed using Test of Within Subjects, and if there was a difference it followed by t-Test. Enamel discoloration tested using Friedman Test and if there was a difference it followed by Wilcoxon Test. The result: There was a significant difference in color and enamel microhardness of the samples after being soaked in 100% strawberry paste. So it can be concluted that Strawberry paste is effective as a bleaching material without decreasing the surface microhardness after 2 weeks application. Application of strawberry paste in 3 weeks shall decrease the enamel surface microhardness.

Mikrostruktur permukaan resin akrilik heat cured setelah kontak larutan cokelat Ab Hafeez; Endanus Harijanto; Asti Meizarini
Jurnal Material Kedokteran Gigi Vol 1 No 1 (2012): JMKG Vol 1 No 1 Maret 2012
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

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Chocolate is one of the most popular food and beverage in the world. This caused by its good taste and anti-stress effect. Chocolate is a processed food from the seed of Theobroma cacao tree, that contain polyphenols. Chocolate beverage are consumed by everyone, as well as dentures wearers. Acrylic resins heat cured are commonly used to make dentures. This materials properties are water absorbent. Phenols chocolate solution are absorbed into the acrylic resin. This prolonged phenomenon i.e chocolate drinks, will cause changes in the physical and chemical properties of this acrylic resin. The aim of this study is to evaluate surface microstructure of the heat cured acrylic resins specimens after contact with various concentrations of chocolate solutions. Four resin acrylic specimens were processed per manufacturer’s instructions into 10x10x1,5 mm and be polished until glossy. Each specimen was submerged into different concentrations of chocolate solution (6g:200ml, 9g:200ml, 12g:200ml) and aquadest as a control for 24 days. Microstructure changes were observed using a scanning electron microscope (SEM) and energy dispersive x-ray spectroscopy (EDS). There were significant microstructural changes for all samples. The surface microstructural changes of the heat cured acrylic resins are notably higher with the increase of chocolate concentration.

Sitotoksisitas Kombinasi Seng-oksida dan Ekstrak Curcuma longa Terhadap Fibroblas Gingiva Manusia Vina Aprilia; Asti Meizarini; Titien Hary Agustantina
Jurnal Material Kedokteran Gigi Vol 8 No 1 (2019): JMKG Vol 8 No 1 Maret 2019
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jmkg.v8i1.335

The use of zinc oxide - eugenol as periodontal dressing is controversial due to the toxic nature of eugenol and Curcuma longa Linn as a safe natural herbal, is expected to replace eugenol function. Previous research has proven that the combination of zinc oxide with Curcuma liquid extract has an antibacterial properties and the use of those as wound dressing through in vivo studies proved to accelerate wound healing. The ingredients applied to the oral cavity must benon-toxic and biocompatible, therefore cytotoxicity testing 1 is required. To determine the cytotoxicity of zinc oxide and Curcuma longa extract combination on human gingival fibroblast cells. Cytotoxicity test of combination of zinc oxide powder with Curcuma liquid extract was done at ratio of 1: 1 (group A) and 2: 1 (group B), Curcuma liquid extract (group C) and zinc oxide powder (group D), which performed on human gingival fibroblast culture-cells (3-5x103 densities) in 96 wells microplate. MTT essay was used to analyzed with IC50 parameters. Statistical analysis using oneway Anova and LSD. The number of living cells in group A = 64.819%, group B = 59.452%, group C = 52.859% and group D = 60.502%. There was no significant difference between the groups of A, B, D but those groups were significantly different to group C. Combination of zinc oxide with Curcuma extract ratio of 1: 1 and 2: 1, zinc oxide powder and Curcuma liquid extract are not toxic to human gingival fibroblast at IC50.

Uji Sitotoksitas Rebusan Buah Lerak (Sapindus rarak DC) Terhadap Sel BHK-21 Atika Rahmadina; Devi Rianti; Asti Meizarini
Jurnal Material Kedokteran Gigi Vol 4 No 1 (2015): JMKG Vol 4 No 1 Maret 2015
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

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Nowadays traditional herbs are become very popular in the medicine world, and also in dentistry. Lerak fruit (Sapindus rarak DC) is one of herbs which is used as a traditional detergent from long time ago. The latest research shows that right now Sapindus rarak DC infusum could be used for an alternative foaming agent as a detergent in tooth paste because of its active substance content that is Saponin. As one of dental health product, this Sapindus rarak DC infusum must be accompanied by non-toxic characteristic before it distributed in the community. The aim of this research is to find out the cytotoxicity of Sapindus rarak DC infusum to BHK-21 cells using MTT assay. This research using post test only control group design. Each group consist of 7 replications in the 96 well microplate. Cultur cells of BHK-21 prepared in media, were divided into 4 group of well. Add 50 µl of Sapindus rarak DC infusum 0,625%; 1,25%; 2,5%; 5% along with BHK-21 cells. Stored the microplate into an incubator for 24 hours. Measurement of cytotoxicity was an optical density or absorbent and read with ELISA reader 620 nm. Value of absorbent in microplate showed the number of living cells in media culture. The increasing concentration of Sapindus rarak DC infusum i.e. 0,625%; 1,25%; 2,5%; 5% would increase the death cells which that means increasing toxicity. The lowest concentration of Sapindus rarak DC infusum has the lowest cytotoxicity potential toward BHK-21 cells using MTT assay. The minimum concentration of Sapindus rarak DC infusum which could be used as an alternative foaming agent in toothpaste and not toxic is 1,25%.

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