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All Journal Dental Journal (Majalah Kedokteran Gigi) Journal of Dentomaxillofacial Science Jurnal Material Kedokteran Gigi
Asti Meizarini
Department Of Dental Material, Faculty Of Dental Medicine, Universitas Airlangga
Dentistry Materials Science & Nanotechnology

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The compressive strength and static biodegradation rate of chitosan-gelatin limestone-based carbonate hydroxyapatite composite scaffold Rianti, Devi; Purnamasari, Alqomariyah Eka; Putri, Rifayinqa Ruyani; Salsabilla, Noor Zain; Faradillah; Munadziroh, Elly; Agustantina, Titien Hary; Meizarini, Asti; Yuliati, Anita; Syahrom, Ardiyansyah
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 3 (2023): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i3.p160-165

Abstract

Background: One of the main components in tissue engineering is the scaffold, which may serve as a medium to support cell and tissue growth. Scaffolds must have good compressive strength and controlled biodegradability to show biological activities while treating bone defects. This study uses Chitosan-gelatin (C–G) with good flexibility and elasticity and high-strength carbonate hydroxyapatite (CHA), which may be the ideal scaffold for tissue engineering. Purpose: To analyze the compressive strength and static biodegradation rate within various ratios of C–G and CHA (C–G:CHA) scaffold as a requirement for bone tissue engineering. Methods: The scaffold is synthesized from C–G:CHA with three ratio variations, which are 40:60, 30:70, and 20:80 (weight for weight [w/w]), made with a freeze-drying method. The compressive strengths are then tested. The biodegradation rate is tested by soaking the scaffold in simulated body fluid for 1, 3, 7, 14, and 21 days. Data are analyzed with a one-way ANOVA parametric test. Results: The compressive strength of each ratio of C–G:CHA scaffold 40:60 (w/w), 30:70 (w/w), and 20:80 (w/w), consecutively, are 4.2 Megapascals (MPa), 3.3 MPa, 2.2 MPa, and there are no significant differences with the p= 0.069 (p>0.05). The static biodegradation percentage after 21 days on each ratio variation of C–G:CHA scaffold 40:60 (w/w), 30:70 (w/w), and 20:80 (w/w) is 25.98%, 24.67%, and 20.64%. One-way ANOVA Welch test shows the result of the p-value as p<0.05. Conclusion: The compressive strength and static biodegradation of the C–G:CHA scaffold with ratio variations of 40:60 (w/w), 30:70 (w/w), and 20:80(w/w) fulfilled the requirements as a scaffold for bone tissue engineering.
Optimization of proteinase K incubation protocol duration during DNA extraction from oral squamous cell carcinoma FFPE samples Meizarini, Asti; Puteri, Astari; Yasan, Yanna Debby Restifanny; Hussaini, Haizal Mohd
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 4 (2023): December
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i4.p233-237

Abstract

Background: Formalin-fixed paraffin-embedded (FFPE) specimen archives are a valuable source of sample material for molecular biological analysis. However, the DNA isolated from FFPE samples is usually low in concentration and fragmented. Thus, it is necessary to optimize the FFPE DNA extraction protocol to obtain the best results. Proteinase K incubation is undoubtedly crucial in DNA extraction procedures, but this step is often not well explained in the manufacturer's manual. Purpose: This study aimed to find the optimal duration for proteinase K incubation protocols to achieve the highest DNA yields. Methods: Fifteen paraffin blocks of Oral Squamous Cell Carcinoma (OSCC) specimens were obtained, and the cancerous areas were microdissected into smaller cuts for DNA extraction. The samples were randomly divided into three groups (n=5) and subjected to three different proteinase K incubation protocols: one-hour incubation at 56ºC as per the manufacturer's instructions (Group I), 24-hour incubation at 56ºC (Group II), and 48 hours at room temperature with an additional four hours at 56ºC (Group III). The extracted DNA was then quantified using a Nanodrop spectrophotometer. The recorded data were analyzed using ANOVA-LSD. Results: The highest DNA concentration was found in Group III (107.74 ± 41.92), which was significantly higher compared to Group II (59.46 ± 30.32) and Group I (6.46 ± 1.97) (p<0.05). Conclusion: In conclusion, modifying the duration of proteinase K incubation protocols can lead to different DNA yield results. In this study, the most optimized protocol for proteinase K incubation, resulting in the highest DNA yields, was 48 hours at room temperature with an additional four hours at 56ºC.
Co-Authors Ab Hafeez Amelia Hartanto Anita Yuliati Atika Rahmadina Devi Rianti Elly Munadziroh Endanus Harijanto, Endanus Faradillah Hussaini, Haizal Mohd Irmawati Irmawati Mardiana Adam Pambudi Santoso T Purnamasari, Alqomariyah Eka Puteri, Astari Putri, Rifayinqa Ruyani Retna Apsari Salsabilla, Noor Zain Syahrom, Ardiyansyah Titien Hary Agustantina Vina Aprilia Wahyu Puri Wardhani Yasan, Yanna Debby Restifanny