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All Journal Jurnal Sain Veteriner Partner JURNAL ILMIAH PETERNAKAN TERPADU JURNAL KAJIAN VETERINER Jurnal Pengabdian kepada Masyarakat Nusantara Buletin Veteriner Udayana
Jois Moriani Jacob
Politeknik Pertanian Negeri Kupang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Effectiveness Comparison Between Young Leaf Extracts Acacia nilotica with Desmanthus virgatus Against Vermicidal Potency of Haemonchus contortus In-vitro I Gusti Komang Oka Wirawan; Aholiab Aoetpah; Jois Moriani Jacob
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60809

Abstract

Acacia nillotica and Desmanthus virgatus are two entopharmacologycal plants that thrives throughout the season in East Nusa Tenggara Province, other Provinces in Indonesia or tropical countries. Extraction of young leaves of Acacia nillotica (EDMAN) and Desmanthus virgatus (EDMDV) contains tannin compound. By pharmacodynamic viewpoint, this extraction has potency as an anthelmintic. Objective: to compare the in-vitro effectivity of young leaves extraction of the two plants as a vermicidal power to combat Haemonchus contortus. Materials: young leaves of Acacia nillotica and Desmanthus virgatus and Haemonchus contortus. Method: The study was grouped into four treatments: EDMAN, EDMDV, Positive control (Albendazole 0,055%) and negative control (aquades).  The concentration of the young leaves extracts are 2.5%, 3.5%, 4.5% out of 0.01 g/mL of extraction. Each treatment was applied to 6 female Haemonchus contortus with four replicates allowing immersion time for 1, 3, 5 or 7 hours. Variable measured and tested was mortality of the H. contortus. The vermicidal effectively was descriptively analysed. The results showed that mortality percentage (vermicidal) treatment of 2.5%, 3.5%, 4.5% EDMAN for 7-hour immersion was 16.7%, 45.8%, 12.5%, respectively. That values for EDMDV for similar concentrations and immersion time was 50%, 33.3%, 12.5%, respectively. Conclusion: EDMDV has a more effective vermicidal power between the two etnopharmacological treatments at 2.5% concentration.
GAMBARAN TOTAL PLATE COUNT (TPC) PADA DAGING SAPI YANG DIAMBIL DI RUMAH POTONG HEWAN (RPH) KOTA KUPANG Jois Moriani Jacob; Erda Eni Rame Hau; Yanse Yane Rumlaklak
Partner Vol 23, No 1 (2018): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v23i1.291

Abstract

The Increasing of beef consumption in Kupang City, shows that there is an increase in people's need for animal protein. However, high meat consumption can also be a threat to health if contaminated by microorganisms.This study aims to calculate the amount of microbial contamination in beef samples taken from Kupang City Slaughterhouse. 30 longissimus dorsi muscle samples were taken from RPH and taken to the Kupang City Veterinary Engineering Unit to be examined using the Total PlateCaount Test (TPC), then the bacterial colonies were calculated using a colony counter.The results of the TPC analysis show that 63.33% of the samples have TPC values that are above the normal standard, while only 36.67% are at normal values.
INTERACTIONS OF OLIVE OIL POLYPHENOLS WITH AB OLIGOMERS Jois Moriani Jacob; Erda Eni Rame Hau
Partner Vol 24, No 2 (2019): Edisi November 2019
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i2.387

Abstract

Neurotoksisitas terutama mempengaruhi patogenesa penyakit Alzheimer (AD). Mekanisme yang diprediksi adalah pembentukan dan pengendapan plak beta amiloid (AB) dalam keadaan agak terlarut dalam jaringan otak. Senyawa polifenol yang dihasilkan oleh minyak zaitun telah dihubungkan dengan treatment dari pembentukan plak AB dengan cara mengikat dan menghambat pembentukan agregat oligomerik beracun yang terlarut.  Studi ini bertujuan untuk menganalisis afinitas pengikatan AB (11-42)- oligomer dengan beberapa senyawa polifenol dan menentukan senyawa yang paling mampu untuk menghambat agregasi AB-oligomer. Metode komputasi digunakan untuk memprediksi kemampuan senyawa polifenol untuk berikatan dengan AB (11-42)-oligomer.  Metode ini termasuk docking, visualisasi 3D, dan analisis struktural.  Hasil menunjukkan hubungan positif yang kuat antara server docking dan skor afinitas pengikatan yang dihasilkan. Hasil lebih lanjut diilustrasikan polifenol dengan kelompok O-H tinggi dari struktur kimia mereka berkorelasi dengan ikatan afinitas yang lebih tinggi untuk AB (11-42)-oligomer.  Hesperidin memiliki jumlah kelompok O-H tertinggi dalam studi dan juga memiliki salah satu nilai ikatan afinitas tertinggi di ketiga server docking. Hal ini menunjukkan hubungan timbal balik antara struktur polifenol dan ikatan afinitas yang dihasilkan.
STUDI KASUS : PROFIL SEL DARAH MERAH ANJING YANG TERINFEKSI BABESIA SP. Yanse Yane Rumlaklak; Jois Moriani Jacob; Aven B Oematan
JURNAL KAJIAN VETERINER Vol 6 No 1 (2018): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v6i1.960

Abstract

Babesia sp. is a protozoa that infects dogs through tick bite and can cause babesiosis. The purpose of blood tests in case dogs is to find out the profile of the dog's red blood cells with babesiosis. On clinical examination found many ticks on the dog's body. Dogs show clinical symptoms of dehydration and paleness in the mucosa. Blood collection is done through the anterior antibrachii cephalica vein. Blood is taken and collected in EDTA tubes. Then the examination is done by examining blood parasites, examining erythrocyte values, examining hemoglobin concentration and hematocrit values. Examination of blood parasites was done by making reticulocyte preparations, while examining erythrocyte values, hemoglobin concentration and hematocrit values were performed using a Vet Scan® HM 5TM machine. The results of examination of reticulocyte preparations showed the existence of Babesia sp infestations with a presentation of 0.68%. Erythrocyte values, hemoglobin concentration and hematocrit values were 3.52x106 // µL, 6.90 g / dL and 23.81%. Based on history, physical examination, clinical and laboratory examination, it can be concluded that the Beagle dog named Loh has microcytic hypochromatic anemia.
IDENTIFIKASI METABOLIT SEKUNDER BRUCEA JAVANICA (L) MERR DI PULAU TIMOR MELALUI UJI FITOKIMIA Jois Moriani Jacob; Yanse Yanne Rumlaklak
JURNAL KAJIAN VETERINER Vol 8 No 1 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i1.1927

Abstract

Brucea javanica (L) Merr is a shrub that has various types of secondary metabolites that have pharmacologic effects. Various studies on this plant in Indonesia have been conducted, but research on secondary metabolites that have the potential as herbal medicines from this plant originating from the island of Timor has never been done. Therefore, this study aims to evaluate secondary metabolites found in Brucea javanica (L) Merr which originates from Timor Island. Samples in the form of roots, stems, leaves, seeds, and flowers of plants are sent to the Indonesian Institute of Sciences (LIPI) to identify these plant species. Plant determination is carried out under the determination procedure in the LIPI ethnobotany laboratory. The plant determination test results showed that the plant samples sent were Brucea javanica (L) Merr. While the phytochemical tests were carried out at the Laboratory of Chemistry at the Faculty of Science and Engineering of the University of Nusa Cendana to identify secondary metabolites from the Brucea javanica (L) Merr plant according to the phytochemical test standards in the Undana Chemistry laboratory. Phytochemical tests show that there are 4 secondary metabolites found in Brucea javanica (L) Merr namely Alkaloids (+), Tannins (+++), Saponins (+), and Triterpenoids (+++) while secondary metabolites such as Flavonoids and Steroids are not contained in this plant.
UJI KARAKTERISTIK SEDIAAN SALEP EKSTRAK ETANOL BUAH MAKASAR (Brucea javanica [L.] Merr) SEBAGAI KANDIDAT SALEP UNTUK LUKA INCISI DAN LUKA DIABETES Jacob, Jois Moriani; Oematan, Aven B.; Maakh, Yorida Febry
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.6614

Abstract

Makasar fruit (Brucea javanica [L]. Merr) has been widely used as herbal medicine to treat various types of diseases through simple processing. However, the use of Makasar fruit drugs with topical application as an ointment to treat incision wounds or diabetic wounds is extremely rare. The purpose of this research is to produce ointment and to assess the physical quality of the ointment made from the ethanolic extract of the Makassar fruit. The ointment used in this study was made with ethanol extract of Makassar fruit, alpha tocopherol, propyl-paraben, and Vaseline album. The data were observed descriptively and using a qualitative method. This study used two ointment concentrations, 15% and 20%, which were tested three times for each physical evaluation (organoleptic test, homogeneity test, dispersibility test, and pH test). The results showed that the ethanol extract of Makassar fruit could be formulated as an ointment, and the physical evaluation of the ointment discovered that the ointment met the organoleptic standards and the homogeneity standards. Furthermore, the pH test revealed that the ointment was safe to use; however, only 1 sample (repeat 1 concentration of 20%) confirmed that it did not meet the pH requirements because it was lower than the standard (acid pH). Furthermore, the dispersion tests indicated that the ointment did not meet the ointments dispersion standard. Conclusion: because the physical examination showed that the ointment met the standard values, the ointment can be made from the ethanol extract of Makassar fruit. However, because the ointment's spreadability is below the standard value, it must be taken into account.
Analisis Metabolit Sekunder Daun Brucea javanica [L] Merr dari Dua Tempat Berbeda di Pulau Timor Yasa, Julita Dewitri Mertha; Jacob, Jois Moriani
JURNAL KAJIAN VETERINER Vol 12 No 1 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i1.15558

Abstract

The leaves of Brucea javanica [L] Merr have long been utilized as an alternative medicine for a variety of illness. Although a number of investigations have been conducted to ascertain the advantages and composition of secondary metabolites in this plant's leaves, nothing has ever been published on the particular study conducted on the island of Timor. The purpose of this study was to ascertain which secondary metabolites were present in the leaves of this plant that was grown in two distinct locations on Timor Island. The findings of this study will be used as an empirical basis for future investigations into the use of herbal remedies. The study's findings demonstrated that while flavonoids and alkaloids weren't present from the plant's ethanol extract, it did contain highly concentrated terpenoids and tannins at both locations. In contrast, saponin content was only discovered in leaves collected from the neighbourhood of Fatumnutu. The spectrophotometer test revealed that the ethanol extract of Bruce planta javanica [L] Merr from Noelbaki Village had a greater secondary metabolite absorption value compared to plant extract from Fatumnutu Village. The absorption values for tannin and alkaloid showed the largest overall variation in absorption levels, varying by around 0.513% and 0.68% for each constituent. In the meantime, there is a 0.513% variation in tannin compounds between the two sites. In order to ascertain the bioactivity of this plant's leaf extract for the creation of herbal medicines in Indonesia, more investigation is required into the variations in saponin levels and absorbency levels of secondary metabolite compounds from the two locations.
Ethanol Extract of Makassar Leaves (Brucea javanica [L.] Merr.) as a Potential Biopesticide against Rhipicephalus sanguineus in Dogs Jacob, Jois Moriani; Oematan, Aven B.
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.20086

Abstract

Rhipicephalus sanguineus adalah ektoparasit yang sering menyerang anjing, menyebabkan masalah kesehatan seperti anemia, iritasi kulit, dan penurunan berat badan. Penggunaan obat sintetis seperti pyrethroid efektif tetapi memiliki efek samping berbahaya bagi hewan, manusia, dan lingkungan. Oleh karena itu, penelitian ini bertujuan mengevaluasi ekstrak etanol daun makassar (Brucea javanica [L.] Merr.) sebagai biopestisida alami untuk mengendalikan R. sanguineus, mengingat kandungan tanin dan terpenoidnya yang berpotensi sebagai antiparasit. Penelitian ini dilakukan di Laboratorium Kesehatan Hewan Politeknik Pertanian Negeri Kupang dari bulan Mei sampai November 2022. Daun Brucea javanica diekstraksi menggunakan etanol 96% dan diuapkan hingga terbentuk ekstrak semipadat, kemudian diencerkan menjadi konsentrasi 10%, 20%, dan 30%, dengan kontrol positif Cypermethrin 100 g/L dan kontrol negatif aquades. Sebanyak 120 kutu dibagi ke dalam kelompok perlakuan dan diamati mortalitasnya setiap jam selama 8 jam. Data dianalisis menggunakan ANOVA dan uji lanjut Duncan. Hasil menunjukkan bahwa semua konsentrasi ekstrak (10%, 20%, dan 30%) menyebabkan mortalitas kutu yang signifikan (P<0,05) dibandingkan kontrol negatif, meskipun tidak berbeda signifikan dengan kontrol positif. Waktu penyemprotan optimal adalah 120 menit, dengan mortalitas tertinggi (1,80±2,111). Efektivitas ekstrak diduga berasal dari kandungan terpenoid yang mengganggu sistem saraf kutu dan tanin yang menghambat metabolisme. Namun, peningkatan konsentrasi tidak secara signifikan meningkatkan mortalitas, menunjukkan bahwa efek ekstrak mungkin telah mencapai titik optimal pada konsentrasi rendah. Ekstrak etanol daun Brucea javanica terbukti efektif sebagai biopestisida terhadap R. sanguineus, terutama pada waktu penyemprotan 120 menit. Namun, diperlukan penelitian lebih lanjut untuk mengisolasi senyawa aktif dan menguji efikasi pada dosis yang lebih tinggi serta metode aplikasi yang lebih baik. Temuan ini menawarkan alternatif alami yang potensial untuk mengurangi ketergantungan pada pestisida sintetis.
Analysis of SGPT and SGOT Levels in Free-Range Chickens Given Starfruit Leaf Extract Moenek, Devi Y. J. A.; Jacob, Jois Moriani; Toelle, Novianti N.; Yasa, Julita D. Mertha; Manoh, Olivia
JURNAL KAJIAN VETERINER Vol 13 No 1 (2025): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v13i1.20718

Abstract

Tujuan dari penelitian ini adalah untuk menentukan kadar enzim Serum Glutamic Oxaloacetic Transaminase (SGOT) dan Serum Glutamic Pyruvate Transaminase (SGPT) pada ayam kampung yang diberi ekstrak daun belimbing wuluh (Averrhoa bilimbi L.). Ekstrak daun belimbing wuluh mengandung metabolit sekunder seperti tanin, saponin, dan flavonoid, yang semuanya memiliki potensi untuk berfungsi sebagai antibakteri alami dan imunostimulan pada unggas. Studi ini menggunakan Rancangan Acak Lengkap (RAL) dengan lima kelompok perlakuan: tiga kelompok ayam yang diberikan ekstrak daun belimbing wuluh dengan dosis 0,25 mL, 0,30 mL, dan 0,35 mL per ekor per hari, dan dua kelompok kontrol (positif untuk antibiotik oksitetrasiklin dan negatif untuk air). Sampel darah diambil setelah periode perlakuan, dan kadar SGOT serta SGPT diukur di laboratorium. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun belimbing tidak memiliki efek signifikan terhadap kadar SGPT ayam kampung (P>0,05). Namun, kadar SGOT bervariasi antar kelompok, dengan nilai tertinggi pada kontrol negatif. Ini menunjukkan bahwa ekstrak daun belimbing tidak bersifat hepatotoksik dan dapat digunakan sebagai alternatif antibiotik alami. Temuan ini mendukung penggunaan ekstrak daun belimbing wuluh dalam pakan ayam kampung sebagai cara untuk mengurangi ketergantungan pada antibiotik sintetis tanpa berdampak negatif pada fungsi hati.
Anatomical and Histological Features of the Tongue Wild Boar (Sus scrofa) from Timor Island Harum, Aurelia Bebonita; Inggrid Trinidad Maha; Nitbani, Heny; Filphin Adolfin Amalo; Selan, Yulfia Nellymalik; Jacob, Jois Moriani
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 13 No. 3 (2025)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v13i3.p875-888

Abstract

The tongue is a complementary organ owned by living things that easily moves and fills the oral cavity when the upper and lower jaw teeth meet. This study aims to determine the anatomical structure and histology of the tongue in wild boar (Sus scrofa) from the Island of Timor. Tongue organs were collected from three healthy wild boars. Animals were slaughtered and necropsied. Macroscopic observations were made; length, width, and thickness were measured, and the tongue tissue was cut into several parts, namely the apex lingua, corpus lingua, and radix lingua, to be fixed in 10% formalin. Furthermore, Hematoxylin-Eosin (HE) staining was performed. The results showed that the dorsal surface has four types of papillae, namely circumvallate papillae, conical papillae, foliate papillae, fungiform papillae, and filiform papillae. The tongue muscles of wild boar were found to be m. longitudinalis, m. transversus, and m. verticalis. Conical papillae are conical with a blunt tip. Foliate papillae are located on the lateral-radix of the tongue. Fungiform papillae have a dome-like oval shape. Filiform papillae have an elongated cylindrical shape with a blunt tip. The size of the filiform papillae at the corpus of the tongue is larger than at the apex of the tongue.  
Co-Authors Agrippina Bele Aholiab Aoetpah Andy Ninu Aven B Oematan Erda Eni Rame Hau Filphin Adolfin Amalo Harum, Aurelia Bebonita I Gusti Komang Oka Wirawan, I Gusti Komang Inggrid Trinidad Maha Julita Dewitri Merthayasa Maakh, Yorida Febry Manoh, Olivia Moenek, Devi Y.J.A. Nitbani, Heny Novianti Neliyani Toelle Selan, Yulfia Nellymalik Stormy Vertygo Yanne Yanse Rumlaklak Yasa, Julita D. Mertha Yasa, Julita Dewitri Mertha