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All Journal Jurnal Sain Veteriner Partner JURNAL KAJIAN VETERINER
Jois Moriani Jacob
Politeknik Pertanian Negeri Kupang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Effectiveness Comparison Between Young Leaf Extracts Acacia nilotica with Desmanthus virgatus Against Vermicidal Potency of Haemonchus contortus In-vitro I Gusti Komang Oka Wirawan; Aholiab Aoetpah; Jois Moriani Jacob
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60809

Abstract

Acacia nillotica and Desmanthus virgatus are two entopharmacologycal plants that thrives throughout the season in East Nusa Tenggara Province, other Provinces in Indonesia or tropical countries. Extraction of young leaves of Acacia nillotica (EDMAN) and Desmanthus virgatus (EDMDV) contains tannin compound. By pharmacodynamic viewpoint, this extraction has potency as an anthelmintic. Objective: to compare the in-vitro effectivity of young leaves extraction of the two plants as a vermicidal power to combat Haemonchus contortus. Materials: young leaves of Acacia nillotica and Desmanthus virgatus and Haemonchus contortus. Method: The study was grouped into four treatments: EDMAN, EDMDV, Positive control (Albendazole 0,055%) and negative control (aquades).  The concentration of the young leaves extracts are 2.5%, 3.5%, 4.5% out of 0.01 g/mL of extraction. Each treatment was applied to 6 female Haemonchus contortus with four replicates allowing immersion time for 1, 3, 5 or 7 hours. Variable measured and tested was mortality of the H. contortus. The vermicidal effectively was descriptively analysed. The results showed that mortality percentage (vermicidal) treatment of 2.5%, 3.5%, 4.5% EDMAN for 7-hour immersion was 16.7%, 45.8%, 12.5%, respectively. That values for EDMDV for similar concentrations and immersion time was 50%, 33.3%, 12.5%, respectively. Conclusion: EDMDV has a more effective vermicidal power between the two etnopharmacological treatments at 2.5% concentration.
GAMBARAN TOTAL PLATE COUNT (TPC) PADA DAGING SAPI YANG DIAMBIL DI RUMAH POTONG HEWAN (RPH) KOTA KUPANG Jois Moriani Jacob; Erda Eni Rame Hau; Yanse Yane Rumlaklak
Partner Vol 23, No 1 (2018): Edisi Juli
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v23i1.291

Abstract

The Increasing of beef consumption in Kupang City, shows that there is an increase in people's need for animal protein. However, high meat consumption can also be a threat to health if contaminated by microorganisms.This study aims to calculate the amount of microbial contamination in beef samples taken from Kupang City Slaughterhouse. 30 longissimus dorsi muscle samples were taken from RPH and taken to the Kupang City Veterinary Engineering Unit to be examined using the Total PlateCaount Test (TPC), then the bacterial colonies were calculated using a colony counter.The results of the TPC analysis show that 63.33% of the samples have TPC values that are above the normal standard, while only 36.67% are at normal values.
INTERACTIONS OF OLIVE OIL POLYPHENOLS WITH AB OLIGOMERS Jois Moriani Jacob; Erda Eni Rame Hau
Partner Vol 24, No 2 (2019): Edisi November 2019
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v24i2.387

Abstract

Neurotoksisitas terutama mempengaruhi patogenesa penyakit Alzheimer (AD). Mekanisme yang diprediksi adalah pembentukan dan pengendapan plak beta amiloid (AB) dalam keadaan agak terlarut dalam jaringan otak. Senyawa polifenol yang dihasilkan oleh minyak zaitun telah dihubungkan dengan treatment dari pembentukan plak AB dengan cara mengikat dan menghambat pembentukan agregat oligomerik beracun yang terlarut.  Studi ini bertujuan untuk menganalisis afinitas pengikatan AB (11-42)- oligomer dengan beberapa senyawa polifenol dan menentukan senyawa yang paling mampu untuk menghambat agregasi AB-oligomer. Metode komputasi digunakan untuk memprediksi kemampuan senyawa polifenol untuk berikatan dengan AB (11-42)-oligomer.  Metode ini termasuk docking, visualisasi 3D, dan analisis struktural.  Hasil menunjukkan hubungan positif yang kuat antara server docking dan skor afinitas pengikatan yang dihasilkan. Hasil lebih lanjut diilustrasikan polifenol dengan kelompok O-H tinggi dari struktur kimia mereka berkorelasi dengan ikatan afinitas yang lebih tinggi untuk AB (11-42)-oligomer.  Hesperidin memiliki jumlah kelompok O-H tertinggi dalam studi dan juga memiliki salah satu nilai ikatan afinitas tertinggi di ketiga server docking. Hal ini menunjukkan hubungan timbal balik antara struktur polifenol dan ikatan afinitas yang dihasilkan.
STUDI KASUS : PROFIL SEL DARAH MERAH ANJING YANG TERINFEKSI BABESIA SP. Yanse Yane Rumlaklak; Jois Moriani Jacob; Aven B Oematan
JURNAL KAJIAN VETERINER Vol 6 No 1 (2018): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v6i1.960

Abstract

Babesia sp. is a protozoa that infects dogs through tick bite and can cause babesiosis. The purpose of blood tests in case dogs is to find out the profile of the dog's red blood cells with babesiosis. On clinical examination found many ticks on the dog's body. Dogs show clinical symptoms of dehydration and paleness in the mucosa. Blood collection is done through the anterior antibrachii cephalica vein. Blood is taken and collected in EDTA tubes. Then the examination is done by examining blood parasites, examining erythrocyte values, examining hemoglobin concentration and hematocrit values. Examination of blood parasites was done by making reticulocyte preparations, while examining erythrocyte values, hemoglobin concentration and hematocrit values were performed using a Vet Scan® HM 5TM machine. The results of examination of reticulocyte preparations showed the existence of Babesia sp infestations with a presentation of 0.68%. Erythrocyte values, hemoglobin concentration and hematocrit values were 3.52x106 // µL, 6.90 g / dL and 23.81%. Based on history, physical examination, clinical and laboratory examination, it can be concluded that the Beagle dog named Loh has microcytic hypochromatic anemia.
IDENTIFIKASI METABOLIT SEKUNDER BRUCEA JAVANICA (L) MERR DI PULAU TIMOR MELALUI UJI FITOKIMIA Jois Moriani Jacob; Yanse Yanne Rumlaklak
JURNAL KAJIAN VETERINER Vol 8 No 1 (2020): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v8i1.1927

Abstract

Brucea javanica (L) Merr is a shrub that has various types of secondary metabolites that have pharmacologic effects. Various studies on this plant in Indonesia have been conducted, but research on secondary metabolites that have the potential as herbal medicines from this plant originating from the island of Timor has never been done. Therefore, this study aims to evaluate secondary metabolites found in Brucea javanica (L) Merr which originates from Timor Island. Samples in the form of roots, stems, leaves, seeds, and flowers of plants are sent to the Indonesian Institute of Sciences (LIPI) to identify these plant species. Plant determination is carried out under the determination procedure in the LIPI ethnobotany laboratory. The plant determination test results showed that the plant samples sent were Brucea javanica (L) Merr. While the phytochemical tests were carried out at the Laboratory of Chemistry at the Faculty of Science and Engineering of the University of Nusa Cendana to identify secondary metabolites from the Brucea javanica (L) Merr plant according to the phytochemical test standards in the Undana Chemistry laboratory. Phytochemical tests show that there are 4 secondary metabolites found in Brucea javanica (L) Merr namely Alkaloids (+), Tannins (+++), Saponins (+), and Triterpenoids (+++) while secondary metabolites such as Flavonoids and Steroids are not contained in this plant.
Co-Authors Aholiab Aoetpah Aven B Oematan Erda Eni Rame Hau I Gusti Komang Oka Wirawan, I Gusti Komang Rumlaklak, Yanse Yane Yanse Yanne Rumlaklak