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Journal : Bulletin of Chemical Reaction Engineering

Solid State Fermentation Parameters Effect on Cellulase Production from Empty Fruit Bunch Vita Wonoputri; Subiantoro Subiantoro; Made Tri Ari Penia Kresnowati; Ronny Purwadi
Bulletin of Chemical Reaction Engineering & Catalysis 2018: BCREC Volume 13 Issue 3 Year 2018 (December 2018)
Publisher : Department of Chemical Engineering - Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.004 KB) | DOI: 10.9767/bcrec.13.3.1964.553-559

Abstract

In this study, agriculture waste palm empty fruit bunch (EFB) was used as carbon/cellulose source in solid state fermentation for cheaper cellulase production. Fermentation operation parameters, such as: solid to liquid ratio, temperature, and pH, were varied to study the effect of those parameters towards crude cellulase activity. Two different fungi organisms, Trichoderma viride and Trichoderma reesei were used as the producers. Extracellular cellulase enzyme was extracted using simple contact method using citrate buffer. Assessment of the extracted cellulase activity by filter paper assay showed that Trichoderma viride is the superior organism capable of producing higher cellulase amount compared to Trichoderma reesei at the same fermentation condition. The optimum cellulase activity of 0.79 FPU/g dry substrate was obtained when solid to liquid ratio used for the fermentation was 1:1, while the optimum fermentation temperature and pH were found to be 30 °C and 5.5, respectively. The result obtained in this research showed the potential of EFB utilization for enzyme production. 
Evaluation of Simultaneous Saccharification and Fermentation of Oil Palm Empty Fruit Bunches for Xylitol Production Khairul Hadi Burhan; Made Tri Ari Penia Kresnowati; Tjandra Setiadi
Bulletin of Chemical Reaction Engineering & Catalysis 2019: BCREC Volume 14 Issue 3 Year 2019 (December 2019)
Publisher : Department of Chemical Engineering - Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (512.24 KB) | DOI: 10.9767/bcrec.14.3.3754.559-567

Abstract

The biological process route of xylitol production from lignocellulosic materials, via enzymatic hydrolysis which is followed by fermentation, offers a more sustainable or greener process than the chemical process route. Both the enzymatic hydrolysis and the fermentation processes are conducted at moderate process condition and thus require less energy and chemicals. However, the process proceeds slower than the chemical one. In order to improve process performance, the enzymatic hydrolysis and the fermentation processes can be integrated as Simultaneous Saccharification and Fermentation (SSF) configuration. This paper discusses the evaluation of SSF configuration on xylitol production from Oil Palm Empty Fruit Bunches (OPEFB). To integrate two processes which have different optimum temperature, the performance of each process at various temperature was first evaluated. Later, SSF was evaluated at various hydrolysis and fermentation time at each optimum temperature. SSF showed better process performance than the separated hydrolysis and fermentation processes. The best result was obtained from configuration with 72 hours of prior hydrolysis followed by simultaneous hydrolysis and fermentation, giving yield of 0.08 g-xylitol/g-OPEFB. 
Modelling Based Analysis and Optimization of Simultaneous Saccharification and Fermentation for the Production of Lignocellulosic-Based Xylitol Ibnu Maulana Hidayatullah; I G B N Makertihartha; Tjandra Setiadi; Made Tri Ari Penia Kresnowati
Bulletin of Chemical Reaction Engineering & Catalysis 2021: BCREC Volume 16 Issue 4 Year 2021 (December 2021)
Publisher : Department of Chemical Engineering - Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.9767/bcrec.16.4.11807.857-868

Abstract

Simultaneous saccharification and fermentation (SSF) configuration offers efficient use of the reactor. In this configuration, both hydrolysis and fermentation processes are conducted simultaneously in a single bioreactor, and the overall processes may be accelerated. However, problems may arise if both processes have different optimum conditions, and therefore process optimization is required. This paper presents a mathematical model over SSF strategy implementation for producing xylitol from the hemicellulose component of lignocellulosic materials. The model comprises the hydrolysis of hemicellulose and the fermentation of hydrolysate into xylitol. The model was simulated for various process temperatures, prior hydrolysis time, and inoculum concentration. Simulation of the developed kinetics model shows that the optimum SSF temperature is 36 °C, whereas conducting prior hydrolysis at its optimum hydrolysis temperature will further shorten the processing time and increase the xylitol productivity. On the other hand, increasing the inoculum size will shorten the processing time further. For an initial xylan concentration of 100 g/L, the best condition is obtained by performing 21-hour prior hydrolysis at 60 °C, followed by SSF at 36 °C by adding 2.0 g/L inoculum, giving 46.27 g/L xylitol within 77 hours of total processing time. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).