Kuwat Triyana
Gadjah Mada University

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Electrical Conductivity of Polyaniline Fiber Synthesized by Interfacial Polymerization and Electrospinning Ngurah Ayu Ketut Umiati; Kamsul Abraha; Kuwat Triyana
Indonesian Journal of Electrical Engineering and Computer Science Vol 5, No 1: January 2017
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijeecs.v5.i1.pp85-89

Abstract

Polyaniline fiber is a promising biosensor material due to the capability of this material as an effective mediator for electron transfer. The polyaniline in fibre has wider surface to increase the electron transfer. In this work, polyaniline structure synthesized by interfacial polymerization was compared to polyaniline structure obtained from electrospinning to get a better fibre structure. Interfacial polymerization was carried out to form a polymerization between the water phase and the organic phase. The water phase was prepared from dopants, initiator and aquadestilata and the organic phase was was made from toluene as an organic solvent and aniline monomer. Electrospinning was conducted by using a dc high voltage 15 kV and 0.5 mm syringe needle to produce fibers from a melt polymer solution taken from interfacial polymerization. The scanning electro microscope results confimed the formation of polyaniline in structure of fiber. Resistance measurement by using LCR meter showed that polyaniline fiber resulted from electrospinning is more conductive than polyaniline fiber formed by interfacial polymerization method.  
Validasi Marker Infeksi Dini Ganoderma pada Kelapa Sawit di Tiga Lokasi Endemik Ganoderma Galuh Wening Permatasari; Mayumi Puspita; Irma Kresnawaty; Agustin Sri Mulyatni; Deden Dewantara Eris; Happy Widiastuti; Kuwat Triyana; Priyono Priyono
Jurnal Penelitian Kelapa Sawit Vol 31 No 1 (2023): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v31i1.195

Abstract

A serious disease that affects the viability of the oil palm industry is basal stem rot, which is caused by Ganoderma. The current level of disease can be viewed as unmanageable, given that the palms were growing in an unfavorable or unsuitable climate. Today, there are numerous approaches to diagnose diseases early, and one of them using molecular methods. Seven genes for early infection markers were effectively generated by a reference's transcriptome study, including LEUCO, ETHYLENE, CHALCONE, ANTHOCYANIDIN, ETHYLENE, MANNOSE, and SENESCENCE. The purpose of this study is to validate and confirm the presence of Ganoderma infections in three endemic oil palm field in Indonesia i.e. Cisalak Baru, Rejosari, and Bekri plantation. This study conducted real time qPCR of RNA from oil palm roots with four different severities of infection. Manual processing of RNA isolation and cDNA synthesis were carried out, to provide quantification expression level. In addition, gene ontology (GO) analysis was also performed in order to explain the roles of each gene tested. The results revealed that CHALCONE is the only marker that consistently elucidate the Ganoderma's early infection appear in three locations. The drawbacks of the analysis results are tightly correlating to the age of oil palm as well as endemic location. GO results declare that seven genes function related to the response of infection. This work was successful in confirming early infection in three places, elucidating the variables influencing the efficacy and sensitivity of molecular detection, and revealing the function and importance of particular genes for detection.
Identification of volatile organic compound of oil palm plants infected with Ganoderma sp. Irma Kresnawaty; Agustin Sri Mulyatni; Deden Dewantara Eris; Tri Panji; Happy Widiastuti; Kuwat Triyana
Menara Perkebunan Vol. 92 No. 1 (2024): 92(1), 2024
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v92i1.534

Abstract

The success of controlling basal stem rot (BSR) disease caused by Ganoderma sp. is mostly determined by the early detection of the fungi. Hence, when the signs of infection begin to appear, plants are generally difficult to rescue since the pathogen infection has spread widely to all parts of the plant. Therefore, early detection through the application of biosensors for Ganoderma sp. infection is absolutely necessary. This study aim was to identify biomarker compounds of Ganoderma sp. infection in oil palm plants with GC-MS for volatile compounds, and LC-MS for non-volatile compounds. The results showed that Ganoderma sp. mycelium produced pyrimidinamine compounds. Meanwhile in early infected nursery plants, were found benzo[h]quinoline, hexaoxa-7,9,11-trisilaheptad, tris-(trimethyl-silyl ester and methyl-tris(trimethyl-siloxy)-silane). Whereas in Bekri, Rejosari, and Adolina plantation, the compound methyl-tris(trimethyl-siloxy)-silane was also found in healthy mature plants. GC-MS test results showed that for early, moderate and severe plants produced several benzene derivative compounds such as ethylbenzene, xylene, and benzaldehyde. These compounds were assumed to be resulted from the breakdown of the lignin structure which build plant cell walls, and have potency to be used as marker compounds for early infected Ganoderma sp. detection. The result of the produced gas quantification concluded that in the produced oil palm plants released less CO2 compared to healthy plants. On the other hand, the NH3 produced was higher than the healthy plants. Meanwhile, two non-volatile compounds were found  that  they  were  only  produced  by  infected trunk and root tissue, namely pseudobrucine and picrasidine.