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All Journal Jurnal Penelitian Kelapa Sawit Menara Perkebunan
Happy Widiastuti
Indonesian Oil Palm Research Institute
Agriculture, Biological Sciences & Forestry

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Validasi Marker Infeksi Dini Ganoderma pada Kelapa Sawit di Tiga Lokasi Endemik Ganoderma Galuh Wening Permatasari; Mayumi Puspita; Irma Kresnawaty; Agustin Sri Mulyatni; Deden Dewantara Eris; Happy Widiastuti; Kuwat Triyana; Priyono Priyono
Jurnal Penelitian Kelapa Sawit Vol 31 No 1 (2023): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v31i1.195

Abstract

A serious disease that affects the viability of the oil palm industry is basal stem rot, which is caused by Ganoderma. The current level of disease can be viewed as unmanageable, given that the palms were growing in an unfavorable or unsuitable climate. Today, there are numerous approaches to diagnose diseases early, and one of them using molecular methods. Seven genes for early infection markers were effectively generated by a reference's transcriptome study, including LEUCO, ETHYLENE, CHALCONE, ANTHOCYANIDIN, ETHYLENE, MANNOSE, and SENESCENCE. The purpose of this study is to validate and confirm the presence of Ganoderma infections in three endemic oil palm field in Indonesia i.e. Cisalak Baru, Rejosari, and Bekri plantation. This study conducted real time qPCR of RNA from oil palm roots with four different severities of infection. Manual processing of RNA isolation and cDNA synthesis were carried out, to provide quantification expression level. In addition, gene ontology (GO) analysis was also performed in order to explain the roles of each gene tested. The results revealed that CHALCONE is the only marker that consistently elucidate the Ganoderma's early infection appear in three locations. The drawbacks of the analysis results are tightly correlating to the age of oil palm as well as endemic location. GO results declare that seven genes function related to the response of infection. This work was successful in confirming early infection in three places, elucidating the variables influencing the efficacy and sensitivity of molecular detection, and revealing the function and importance of particular genes for detection.
Soil fungal diversity profile of the sugarcane plantations in Lampung and South Sumatra, Indonesia Muhammad Abdul Aziz; Donny Nugroho Kalbuadi; Galuh Wening Permatasari; Happy Widiastuti
E-Journal Menara Perkebunan Vol. 94 No. 1 (2026): 94(1), 2026
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v94i1.707

Abstract

As a high-value commodity, sugarcane harvesting areas in Indonesia have been declining year after year. Metagenomic studies on sugarcane plantation land are expected to provide an overview of land suitability for sustainable sugarcane production. This study investigated soil fungal communities in two sugarcane plantations, Bunga Mayang (BUMA) and Cinta Manis (CIMA), in Lampung and South Sumatra, respectively, using high-throughput ITS1 gene sequencing. Chemical and biological analyses were conducted on soils with depths of 0-20 cm and 20-      40 cm on plant cane (PC) and ratoon cane (RC) categories. The results showed that plantation land in South Sumatra (CIMA) had higher organic carbon and total nitrogen concentrations than in Lampung (BUMA). However, in several other parameters, such as P2O5, K2O, and microbial population, the BUMA farm has higher values with lower pH. Metagenomic analysis revealed the differences in microbial dominance between the plantation areas. Sugarcane fields in Lampung were dominated by the genus Protrudomyces, Coralloidiomyces, and Spizellomyces, while South Sumatra was dominated by the genus Lichtheimia, Phyctochytrium, and Spizellomyces. In the RC category, Lichtheimia corymbifera is the most dominant species in both the BUMA (RC-B) and CIMA (RC-C) plantations. However, in the PC category, BUMA (PC-B) is dominated by Protrudomyces lateralis, meanwhile CIMA (PC-C) is dominated by Phlyctochytrium californicum. The alpha diversity values of the two areas did not significantly different, with low Trichoderma populations. Thus, sugarcane fields in the two locations differ in microbial composition, which is thought to be influenced by different technical cultures and environmental conditions.
Co-Authors Agustin Sri Mulyatni Deden Dewantara Eris Donny Nugroho Kalbuadi Galuh Wening Permatasari Irma Kresnawaty Kuwat Triyana Mayumi Puspita Muhammad Abdul Aziz Priyono Priyono