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All Journal Jurnal Penelitian Kelapa Sawit Indonesian Sugar Research Journal
Agustin Sri Mulyatni
Indonesian Oil Palm Research Institute
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Electrical & Electronics Engineering Energy Environmental Science

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Validasi Marker Infeksi Dini Ganoderma pada Kelapa Sawit di Tiga Lokasi Endemik Ganoderma Galuh Wening Permatasari; Mayumi Puspita; Irma Kresnawaty; Agustin Sri Mulyatni; Deden Dewantara Eris; Happy Widiastuti; Kuwat Triyana; Priyono Priyono
Jurnal Penelitian Kelapa Sawit Vol 31 No 1 (2023): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v31i1.195

Abstract

A serious disease that affects the viability of the oil palm industry is basal stem rot, which is caused by Ganoderma. The current level of disease can be viewed as unmanageable, given that the palms were growing in an unfavorable or unsuitable climate. Today, there are numerous approaches to diagnose diseases early, and one of them using molecular methods. Seven genes for early infection markers were effectively generated by a reference's transcriptome study, including LEUCO, ETHYLENE, CHALCONE, ANTHOCYANIDIN, ETHYLENE, MANNOSE, and SENESCENCE. The purpose of this study is to validate and confirm the presence of Ganoderma infections in three endemic oil palm field in Indonesia i.e. Cisalak Baru, Rejosari, and Bekri plantation. This study conducted real time qPCR of RNA from oil palm roots with four different severities of infection. Manual processing of RNA isolation and cDNA synthesis were carried out, to provide quantification expression level. In addition, gene ontology (GO) analysis was also performed in order to explain the roles of each gene tested. The results revealed that CHALCONE is the only marker that consistently elucidate the Ganoderma's early infection appear in three locations. The drawbacks of the analysis results are tightly correlating to the age of oil palm as well as endemic location. GO results declare that seven genes function related to the response of infection. This work was successful in confirming early infection in three places, elucidating the variables influencing the efficacy and sensitivity of molecular detection, and revealing the function and importance of particular genes for detection.
Identifikasi Molekuler Trichoderma sp. Isolat T10 ISRI sebagai Agen Hayati Penyakit Busuk Akar dan Pangkal Batang Xylaria Wiwit Wicaksono Jati; Ari Kristini; Agustin Sri Mulyatni; Abdul Latief Abadi
Indonesian Sugar Research Journal Vol 3, No 1 (2023): Indonesian Sugar Research Journal
Publisher : Pusat Penelitian Perkebunan Gula Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54256/isrj.v3i1.94

Abstract

Penyakit busuk akar dan pangkal batang pada tebu merupakan penyakit yang memiliki arti penting secara ekonomi.  Salah satu agen hayati yang berpotensi mengendalikan penyakit busuk akar dan pangkal batang Xylaria adalah Trichoderma sp. Isolat T10 ISRI memiliki aktivitas enzim kitinase yang tinggi sehingga dapat digunakan sebagai agen hayati. Identifikasi isolate T10 ISRI bertujuan agar potensi lain dari isolate T10 ISRI dapat diktahui. Tujuan dari penelitian ini adalah untuk mengidentifikasi isolat T10 ISRI sampai dengan tingkat spesies melalui identifikasi secara molekuler. Metode identifikasi dilakukan dengan sequencing DNA melalui BLAST dan pembuatan pohon filogenik. Hasil penelitian menunjukkan bahwa spesies isolat T10 ISRI yaitu Trichoderma asperellum dengan kesamaan 100% dengan strain Trichoderma asperellum T sum 66 dari data NCBI dan kedekatan dalam kelompok 0,00001 dari pada jarak diluar kelompok yaitu 0,3287.
Validasi Marker Infeksi Dini Ganoderma pada Kelapa Sawit di Tiga Lokasi Endemik Ganoderma Galuh Wening Permatasari; Mayumi Puspita; Irma Kresnawaty; Agustin Sri Mulyatni; Deden Dewantara Eris; Happy Widiastuti; Kuwat Triyana; Priyono Priyono
Jurnal Penelitian Kelapa Sawit Vol 31 No 1 (2023): Jurnal Penelitian Kelapa Sawit
Publisher : Pusat Penelitian Kelapa Sawit

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iopri.jur.jpks.v31i1.195

Abstract

A serious disease that affects the viability of the oil palm industry is basal stem rot, which is caused by Ganoderma. The current level of disease can be viewed as unmanageable, given that the palms were growing in an unfavorable or unsuitable climate. Today, there are numerous approaches to diagnose diseases early, and one of them using molecular methods. Seven genes for early infection markers were effectively generated by a reference's transcriptome study, including LEUCO, ETHYLENE, CHALCONE, ANTHOCYANIDIN, ETHYLENE, MANNOSE, and SENESCENCE. The purpose of this study is to validate and confirm the presence of Ganoderma infections in three endemic oil palm field in Indonesia i.e. Cisalak Baru, Rejosari, and Bekri plantation. This study conducted real time qPCR of RNA from oil palm roots with four different severities of infection. Manual processing of RNA isolation and cDNA synthesis were carried out, to provide quantification expression level. In addition, gene ontology (GO) analysis was also performed in order to explain the roles of each gene tested. The results revealed that CHALCONE is the only marker that consistently elucidate the Ganoderma's early infection appear in three locations. The drawbacks of the analysis results are tightly correlating to the age of oil palm as well as endemic location. GO results declare that seven genes function related to the response of infection. This work was successful in confirming early infection in three places, elucidating the variables influencing the efficacy and sensitivity of molecular detection, and revealing the function and importance of particular genes for detection.
Co-Authors Abdul Latief Abadi Ari Kristini Deden Dewantara Eris Galuh Wening Permatasari Happy Widiastuti Irma Kresnawaty Kuwat Triyana Kuwat Triyana Mayumi Puspita Mayumi Puspita Priyono Priyono Wiwit Wicaksono Jati