F.M. Suhartati
Universitas Jenderal Soedirman

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PENGGUNAAN AIR SUSU SAPI YANG KAYA ASAM LEMAK LINOLEAT TERKOJUGASI SEBAGAI UPAYA UNTUK KENDALI METABOLIK(STUDI KASUS PADA PENDERITA DISLIPIDEMIA) Suhartati, F.M.; Mulyanto, Djoko
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

A study that aims to produce milk rich in conjugated linoleic acids and testing the milk for metabolic control has been implemented. As a material for producing milk are 5 female dairy cattle production. Cow feed consists of grass and concentrate with a ratio of 60:40. The method used is the pseudo eksperimental and the design was completely randomized design. The treatments tested, namely P1 = patients with dyslipidemia who were given simvastatin 10 mg / person / day, P 2 = patients with dyslipidemia who were given simvastatin 10 mg / person / day, and drinking dairy milk 300 ml / person / day, P3 = Patients dyslipidemia that drinking dairy milk 300 ml / day, P4 = normal cholesterol levels, drinking dairy milk 300 ml / day, P5 = normal cholesterol levels, without simvastatin and without drinking milk (as a negative control). Each treatment was repeated 6 times, so there were 30 respondents. As the respondent is a university lecturer and a female employee of General Sudirman, and SMA Veteran teachers, aged 40-60 years, who had suffered from dyslipidemia and non dyslipidemia. Before and after given treatment, respondents have blood drawn, and then measured the content of trigiserida, total cholesterol, LDL-cholesterol, HDL-cholesterol. Administration of simvastatin and milk implemented for a month. Data were analyzed using analysis of variance followed by orthogonal contrast test (Steel and Torrie, 1993). Based on these results it can be concluded that the respondents as negative control, has increased total cholesterol, triglycerides and LDLcholesterol and decreased HD-cholesterol. Consuming dairy milk 300 ml / person / day in combination with simvastatin 10 mg / person / day can lower-cholesterol, triglycerides, LDL and increase HDL-cholesterol. For patients with dyslipidemia, consume dairy milk 300 ml / person / day can lower LDL cholesterol and triglycerides and increases HDL-cholesterol. In an effort to reduce total cholesterol, triglycerides, LDL-cholesterol and increasing HDLcholesterol, patients with dyslipidemia early to do with the consumption of milk rich in conjugated linoleic fatty acids 300 ml / person / day and 10 mg simvastatin / person / day.
Proteksi Bungkil Kedelai dengan Ekstrak Daun Mahoni terhadap Produk Fermentasi Rumen dan Kecernaan In vitro Ifani, Merryafinola; Suhartati, F.M.; Rimbawato, Efka Aris; Subagyo, Yusuf; Syamsi, Afduha Nurus; Widodo, Hermawan Setyo
Jurnal Agripet Vol 24, No 1 (2024): Volume 24, No. 1, April 2024
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v24i1.20419

Abstract

ABSTRACT. Tujuan penelitian ini adalah untuk mengetahui pengaruh proteksi bungkil kedelai menggunakan ekstrak daun mahoni terhadap produk VFA total, konsentrasi N-NH3, kecernaan bahan kering, dan kecernaan bahan organik secara in vitro. Bahan yang digunakan adalah bungkil kedelai, daun mahoni, cairan rumen sapi potong, serta reagen untuk analisis konsentrasi amonia dan VFA. Penelitian dilakukan dalam tiga tahap yaitu ekstraksi mahoni, proteksi protein dengan ekstrak mahoni, dan tahap in vitro. Penelitian bersifat eksperimental dengan tiga tahap, yaitu ekstraksi mahoni menggunakan metode soxhlet, pencampuran ekstrak mahoni dengan bungkil kedelai, dan uji kecernaan menggunakan metode in vitro. Hasil penelitian dianalisis menggunakan rancangan acak lengkap dengan 4 perlakuan dan 6 ulangan sehingga terdapat 24 unit percobaan. Perlakuan terdiri dari P0 (bungkil kedelai + ekstrak mahoni 0%), P1 (bungkil kedelai + ekstrak mahoni 1,5%), P2 (bungkil kedelai + ekstrak mahoni 3%) dan P3 (bungkil kedelai + ekstrak mahoni 4,5%). Pengukuran VFA total menggunakan metode distilasi uap dan N-NH3 dengan teknik difusi mikro Conway. Penelitian dilakukan di Laboratorium Ilmu Nutrisi dan Makanan Ternak (INMT) Fakultas Peternakan, Universitas Jenderal Soedirman. Pengukuran hasil tersebut dianalisis secara statistik dengan analisis variansi, jika terdapat perbedaan lebih lanjut diuji menggunakan ortogonal polinomial. Hasil penelitian menunjukkan bahwa perlindungan bungkil kedelai dengan ekstrak mahoni berpengaruh nyata terhadap produk VFA total, N-NH3, kecernaan bahan kering dan kecernaan bahan organik (P0,05). Pemberian bungkil kedelai terproteksi ekstrak daun mahoni berpengaruh secara kubik dengan persamaan berturut-turut Y= 99,67 36,44X + 18,19X2 2,12X3 (R= 0,98); Y= 14 + 0,798X 0,370X2 + 0,055X3 (R= 0,66); Y= 60,30 - 2,89X + 2,67X2 - 0,38X3 (R= 0,65); dan Y= 59,58 2,65X + 1,79X2 0,19X3 (R= 0,91). Berdasarkan VFA total, konsentrasi N-NH3, kecernaan bahan kering, dan kecernaan bahan organik disimpulkan bahwa penambahan ekstrak mahoni taraf 3% efisien.(Protection of soybean meal with mahogany leaf extract on rumen fermentation products and in vitro digestibility)ABSTRAK. The aim of this study was to determine the protective effect of soybean meal using mahogany leaf extract on VFAs product, N-NH3 concentration, dry matter digestibility, and organic matter digestibility in vitro. The materials used were mahogany leaves, beef cattle rumen fluid, and reagents for analysis of ammonia and VFA concentrations. The research was conducted in three stages, mahogany extraction, protein protection with mahogany extract, and in vitro stages. The research was experimental with in vitro method using a completely randomized design with 4 treatments and 6 replications so that there were 24 experimental units. The treatments consisted of P0 (soybean meal + 0% mahogany extract), P1 (soybean meal + 1.5% mahogany extract), P2 (soybean meal + 3% mahogany extract) and P3 (soybean meal + 4.5% mahogany extract). Measurement of total VFA using the steam distillation method and N-NH3 with the Conway micro diffusion technique (Smith et al., 2005). The research was carried out at the Animal Nutrition and Forage Science Laboratory (INMT), Faculty of Animal Science, Jenderal Soedirman University. Measurement The results were analyzed statistically by analysis of variance, if there were further differences tested using orthogonal polynomial. The results showed that the protection of soybean meal with mahogany extract significantly affected the total VFA product, N-NH3, dry matter digestibility and organic matter digestibility (P 0.05). It can be concluded that the addition of 3% level mahogany extract is efficient in increasing the total VFA product, N-NH3 concentration, dry matter digestibility, and organic matter digestibility.