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Journal : Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan

Uji selektivitas fraksi Rf < 0,5 ekstrak MeOH biji putat air (Barringtonia racemosa) terhadap ikan mujair (Oreochromis mossambicus) Musri Musman; Sofia S; Adli W Perdana
Depik Vol 1, No 2 (2012): August 2012
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.764 KB) | DOI: 10.13170/depik.1.2.50

Abstract

Abstract. The objective of the present study was to evaluate the selectivity of the Rf 0.5 fraction of MeOH extract of putat air kernels (Barringtonia racemosa) on golden snail (Pomacea canaliculata) and tilapia (Oreochromis mossambicus). The research was conducted on November 2011 to February 2012 at Chemical Laboratory of Teacher Training and Education Facultyand Marine Chemical Laboratory of Coordinatorate of Marine and Fisheries of Syiah Kuala University. Thin-layer chromatography was used as the separation technique towards component compounds in the extract samples. The research was used five levels concentration of Rf 0.5 fraction of MeOH solution (20 ppm, 40 ppm, 60 ppm, 80 ppm, 100 ppm) with three repetitions. Mortality rates of golden snails and tilapia were detected when they were exposure to Rf 0.5 fraction of MeOH extract of B. racemosa kernels. The data were analyzed by Probit, and selectivity value (S) was calculated by Feng and Wang formula. The results revealed that Rf 0.5 fraction of MeOH extract of putat air kernels was selective to golden apple snails.Key words. Thin-layer chromatography, mortality, Probit, and moluscida
Selektivitas fraksi Rf < 0,5 ekstrak etil asetat (EtOAc) biji putat air (Barringtonia racemosa)terhadap keong mas (Pomacea canaliculata) dan ikan lele lokal (Clarias batrachus) Musri Musman; Sofia F; Viqqi Kurnianda
Depik Vol 1, No 2 (2012): August 2012
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.362 KB) | DOI: 10.13170/depik.1.2.35

Abstract

Abstract. Research to determine the selectivity of fraction Rf 0.5 of ethyl acetate extract (EtOAc) of putat air kernel (Barringtonia racemosa) on golden apple snail(Pomacea canaliculata) and local catfish (Clarias batrachus) was conducted in February 2012 at the Laboratory of Chemistry of Teacher Training and Education Faculty and Laboratory of Marine Chemistry of Coordinatorate of Marine and Fisheries of Syiah Kuala University. Thin-layer chromatography was used to separate components in the extract samples, and testing of biological activity based on the procedures recommended by FAO. Golden apple snail and local catfish mortality data were analyzed using Probit program, and value of selectivity (S) was processed according to the Wang and Feng’s formula.The values of LC50 of the fraction Rf 0.5 were 29.26 ppm for P. canaliculata and 44.47 ppm for C. batracus. The selectivity value for the tested organisms was 1.51. This study revealed that the tested extract has bioactive property as molluscicide of P. canaliculata.Key words. Biological activity, thin-layer chromatography, mortality, bioactive property, molluscicide, LC50.
Uji selektivitas fraksi Rf < 0,5 ekstrak MeOH biji putat air (Barringtonia racemosa) terhadap ikan mujair (Oreochromis mossambicus) Musri Musman; Sofia S; Adli W Perdana
Depik Vol 1, No 2 (2012): August 2012
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.1.2.50

Abstract

Abstract. The objective of the present study was to evaluate the selectivity of the Rf 0.5 fraction of MeOH extract of putat air kernels (Barringtonia racemosa) on golden snail (Pomacea canaliculata) and tilapia (Oreochromis mossambicus). The research was conducted on November 2011 to February 2012 at Chemical Laboratory of Teacher Training and Education Facultyand Marine Chemical Laboratory of Coordinatorate of Marine and Fisheries of Syiah Kuala University. Thin-layer chromatography was used as the separation technique towards component compounds in the extract samples. The research was used five levels concentration of Rf 0.5 fraction of MeOH solution (20 ppm, 40 ppm, 60 ppm, 80 ppm, 100 ppm) with three repetitions. Mortality rates of golden snails and tilapia were detected when they were exposure to Rf 0.5 fraction of MeOH extract of B. racemosa kernels. The data were analyzed by Probit, and selectivity value (S) was calculated by Feng and Wang formula. The results revealed that Rf 0.5 fraction of MeOH extract of putat air kernels was selective to golden apple snails.Key words. Thin-layer chromatography, mortality, Probit, and moluscida
Selektivitas fraksi Rf < 0,5 ekstrak etil asetat (EtOAc) biji putat air (Barringtonia racemosa)terhadap keong mas (Pomacea canaliculata) dan ikan lele lokal (Clarias batrachus) Musri Musman; Sofia F; Viqqi Kurnianda
Depik Vol 1, No 2 (2012): August 2012
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.1.2.35

Abstract

Abstract. Research to determine the selectivity of fraction Rf 0.5 of ethyl acetate extract (EtOAc) of putat air kernel (Barringtonia racemosa) on golden apple snail(Pomacea canaliculata) and local catfish (Clarias batrachus) was conducted in February 2012 at the Laboratory of Chemistry of Teacher Training and Education Faculty and Laboratory of Marine Chemistry of Coordinatorate of Marine and Fisheries of Syiah Kuala University. Thin-layer chromatography was used to separate components in the extract samples, and testing of biological activity based on the procedures recommended by FAO. Golden apple snail and local catfish mortality data were analyzed using Probit program, and value of selectivity (S) was processed according to the Wang and Feng’s formula.The values of LC50 of the fraction Rf 0.5 were 29.26 ppm for P. canaliculata and 44.47 ppm for C. batracus. The selectivity value for the tested organisms was 1.51. This study revealed that the tested extract has bioactive property as molluscicide of P. canaliculata.Key words. Biological activity, thin-layer chromatography, mortality, bioactive property, molluscicide, LC50.