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THE POTENCY OF Bacillus sp. AND Pseudomonas sp. AS BIOLOGICALCONTROL AGENTS AGAINST CORN LEAF BLIGHT DISEASE CAUSED BY Pantoea sp. Javandira, Cokorda; Aini, Luqman Qurata; Sugiharto, Ariffin Noor; Abadi, Abdul Latief
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya and Indonesian Agronomic Assossiation

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One of new biotic constraints in corn production in Indonesia is leaf blight disease caused by Pantoea sp. which is needed to be controlled. The purpose of this research is to study the potential of Bacillus sp. and Pseudomonas sp. as biological control agents against corn leaf blight caused by Pantoea sp. The results showed that all bacterial strains of Bacillus sp. and Pseudomonas sp. have potential in inhibiting the growth of Pantoea sp. by showing the clear zone on the agar plate. The antibiosis types are bactercide or bacteriostatic. On pot experiment all bacterial strains showed the reduction of the disease incidence at the same level compared with that of bactericide streptomycin suphate. All bacterial strains as well as bactericide could reduce the disease incidence at 18-24% compared with that of control (aquades treatment only). The results suggest that all bacterial strains are potential as biological control agent against leaf blight disease on corn leaf caused by Pantoea sp.Keywords: Biological control, Bacillus sp., Pseudomonas sp. and Pantoea sp.

Pemanfaatan Rhizobakteri dari Gulma di UB Forest sebagai Agen Antagonis Penyakit Layu Bakteri pada Kentang Junda Fauzul Izza; Luqman Qurata Aini; Restu Rizkyta Kusuma
Biotropika: Journal of Tropical Biology Vol 6, No 2 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2018.006.02.03

Penyakit layu bakteri yang diakibatkan oleh patogen Ralstonia solanacearum merupakan kendala yang sering terjadi pada budidaya tanaman kentang. Penelitian bertujuan untuk mendapatkan rhizobakteri yang efektif mengendalikan penyakit layu bakteri dan meningkatkan pertumbuhan pada tanaman kentang. Penelitian dilaksanakan di Laboratorium Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya, UB Forest, dan Desa Tulungrejo Kecamatan Bumiaji, Kota Batu. Rhizobakteri diisolasi dari perakaran gulma di UB Forest kemudian dilakukan pengujian aktivitas sebagai pemicu pertumbuhan. Rhizobakteri yang terpilih dilakukan pengujian sifat antagonis terhadap R. solanacearum secara in vitro dan penekanan terhadap angka kejadian penyakit serta pertumbuhan tanaman kentang. Identifikasi isolat dilakukan secara fisiologi, biokimia dan molekuler. Hasil eksplorasi didapatkan 20 isolat rhizobakteri yang bersifat antagonis terhadap R. solanacearum. Isolat AGR 2 memiliki diameter penghambatan yang sama dengan bakterisida secara in vitro. Secara in vivo isolat bakteri AGR 1, AGR 2 dan EPT 9 dapat meningkatkan rerata jumlah daun lebih tinggi dibandingkan dengan bakterisida pada 1 dan 2 MSA (minggu setelah aplikasi). EPT 9 mampu menekan angka kejadian penyakit layu bakteri 55,6 % setelah 5 MSA dan meningkatkan berat umbi sebesar 59,3 % lebih tinggi dari perlakuan kontrol. Isolat AGR 2 diketahui sebagai Pseudomonas aeruginosa dan EPT 9 merupakan Bacillus cereus.

The Potential of Endophytic Fungi in Promoting Rice Plant Growth and Suppressing Blast Disease Novia Dwi Putri; Anton Muhibuddin; Luqman Qurata Aini
Journal of Tropical Plant Protection Vol 2, No 2 (2021)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2021.002.2.2

Endophytic fungi are fungi that inhabit the internal tissues of plant and belong to a group of microorganisms reported to have beneficial effects on plants. In this study, three endophytic fungi isolated from rice plant tissue were identified as Trichoderma asperellum, Curvularia chiangmaiensis, dan Fusarium solani. These three fungi have the ability to produce IAA between 3.03 to 6.00 µg/mL. For phosphate solubility assay, all endophytic fungi showed a clear zone around the colonies in Pikovskaya medium and could dissolve phosphate in the range of 2.74 to 17.61 µg/mL. In addition, in vivo observations of the inoculation endophytic fungi can reduce the intensity of blast disease in rice plant. So that, it can be seen that the three fungal isolates can become plant growth promoting fungi and can also used as bicontrol agents for blast disease in rice plant.

CHARACTERIZATION OF BACTERIAL PATHOGEN CAUSING WILT AND LEAF BLIGHT ON CORN (Zea mays) BY PHYSIOLOGICAL, BIOCHEMICAL AND MOLECULAR METHODS Lilis Suryani; Luqman Qurata Aini; Ariffin Noor Sugiharto; Abdul Latief Abadi
AGRIVITA, Journal of Agricultural Science Vol 34, No 3 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i3.169

In 2011, we found a new bacterial disease characterized by wilt, dwarf and blight symptoms on sweet corn in Batu, East Java, Indonesia. The objective of this study is to characterize the causal agent of the disease. In this study, several assays were conducted, including hypersensitive response, pathogenicity, physiological and biochemical characteristics, PCR detection using two specific species primer pairs for Pantoea stewartii pv. stewartii, and homology analysis of 16S rRNA gene sequence. Four Gram-negative, non-motile, facultative anaerobic bacteria were isolated from the diseased tissue. Only two strains, BD1 and BB2, gave positive result in hypersensitive reaction, pathogenicity, and Koch’s postulate assays. BB2 and BD1 strains also showed positive results in the PCR amplification using specific primers derived from the P. stewartii subsp. stewartii 16-23S gene region of but showed negative result when using primers derived from P. stewartii subsp. stewartii hrpS gene region. Sequence of partial 16S rRNA gene of BD1 and BB2 showed highest homology at 96% to P. stewartii subsp. stewartii strain ATCC 8199 (NR. 044800.1). This results suggest that bacterial pathogens isolated from sweet corn in Batu were strains of Pantoea spp. Keywords : Zea mays, Pantoea spp, wilt, leaf blight, PCR <w:LsdException Locked="false" Priority="70" SemiHidde

THE POTENCY OF Bacillus sp. AND Pseudomonas sp. AS BIOLOGICALCONTROL AGENTS AGAINST CORN LEAF BLIGHT DISEASE CAUSED BY Pantoea sp. Cokorda Javandira; Luqman Qurata Aini; Ariffin Noor Sugiharto; Abdul Latief Abadi
AGRIVITA, Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i2.291

One of new biotic constraints in corn production in Indonesia is leaf blight disease caused by Pantoea sp. which is needed to be controlled. The purpose of this research is to study the potential of Bacillus sp. and Pseudomonas sp. as biological control agents against corn leaf blight caused by Pantoea sp. The results showed that all bacterial strains of Bacillus sp. and Pseudomonas sp. have potential in inhibiting the growth of Pantoea sp. by showing the clear zone on the agar plate. The antibiosis types are bactercide or bacteriostatic. On pot experiment all bacterial strains showed the reduction of the disease incidence at the same level compared with that of bactericide streptomycin suphate. All bacterial strains as well as bactericide could reduce the disease incidence at 18-24% compared with that of control (aquades treatment only). The results suggest that all bacterial strains are potential as biological control agent against leaf blight disease on corn leaf caused by Pantoea sp.Keywords: Biological control, Bacillus sp., Pseudomonas sp. and Pantoea sp.

MICROBIAL COMMUNITY ASSOCIATED WITH AMBROSIA BEETLE, Euplatypus parallelus ON SONOKEMBANG, Pterocarpus indicus IN MALANG Hagus Tarno; Erfan Dani Septia; Luqman Qurata Aini
AGRIVITA, Journal of Agricultural Science Vol 38, No 3 (2016): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i3.628

Recently, most of sonokembang, Pterocarpus indicus trees are dying in Malang. In 2012, the death rate of trees reached ca. 11%. In addition, death of trees spread to other regencies in East Java. Euplatypus parallelus is a specific species of ambrosia beetles that were the causal agents to the dying and wilting of sonokembang trees in Malang. Wilting is caused mainly by the pathogenic fungi carried by ambrosia beetles. To confirm the microbial communities related to E. parallelus that attack sonokembang, E. parallelus and some attacked trees were collected in Malang city. Isolation and identification of these species were conducted at the Laboratory of Mycology, Faculty of Agriculture, University of Brawijaya and Laboratory of Molecular Biology, Islamic State University, Malang. Results showed that there were nine microbes including five genera of fungi, two genera of yeasts and one genus of bacterium were identified. The microbial communities that were found namely Aspergillus spp., Penicillium spp., Trichoderma spp., Fusarium spp., Acremonium spp., Gliocladium spp. (fungi), Streptomyces spp. (bacteria), Saccharomyces spp., and Candida spp. (yeast).

IDENTIFICATION OF BACTERIAL WILT AND LEAF BLIGHT DISEASE ON MAIZE (Zea mays) FOUND IN KEDIRI, INDONESIA Luqman Qurata Aini; Lilis Suryani; Arifin Noor Sugiharto; Abdul Latief Abadi
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.245

Recently, a new bacterial disease of maize (Zea mays) was observed in Kediri, East Java, Indonesia. Infected plants showed wilt symptoms occasionally accompanied by leaf blight. This study aims to characterize the causal agent of bacterial wilt and leaf blight of maize observed in Kediri. Gram-negative, facultative anaerobic bacteria were isolated from the diseased tissues. All bacterial strains (KD1A, KB2A, KD1, KD4, KB1) gave positive result both in the hypersensitive response and pathogen-nicity assays. However, only KD1 and KB1 strains could be re-isolated from the diseased tissues. Based on several physiological and biochemical assays, the bacteria resembled Pantoea agglomerans. Moreover, the strains showed negative result on PCR amplification using HRP1d and HRP3r, a primer pair specific for detection of P. stewartii subsp. stewartii. Analysis of 16S rRNA gene sequence of KD1 and KB1 showed highest homology at 88% and 90%, respectively to P. agglomerans strain DSM 3493 (NR 0419781). The homology values were too low to conclude that the bacteria were similar to P. agglomerans. These results suggest that bacterial pathogens isolated from maize in Kediri were strains of Pantoea sp. Keywords: maize, Pantoea sp., wilt, leaf blight

PENGARUH APLIKASI Pyraclostrobin TERHADAP SERANGAN PENYEBAB PENYAKIT BULAI PADA LIMA VARIETAS JAGUNG (Zea mays) Novie Utami Asputri; Luqman Qurata Aini; Abdul Latief Abadi
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 1 No. 3 (2013)
Publisher : Universitas Brawijaya

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Jagung merupakan makanan pokok bagi penduduk Indonesia kedua setelah padi. Kendala biotik dan abiotik sering muncul dalam produksi jagung nasional sehingga produktivitasnya rendah. Penyakit bulai adalah penyakit penting pada tanaman jagung yang merupakan kendala utama pada budidaya tanaman jagung di Indonesia. Penyakit bulai disebabkan oleh infeksi jamur patogen Peronosclerospora sp. Pyraclostrobin merupakan fungisida dari kelompok strobilurin yang digunakan untuk melindungi tanaman yang diakibatkan oleh jamur. Pyraclostrobin telah dilaporkan efektif mengendalikan penyebab penyakit karat daun (Puccinia sorghi Schw), hawar daun (Exserohilum turcicum (Pass) Leonard et Suggs), bercak daun (Bipolaris maydis (Nisik) Shoemaker), dan busuk batang pada tanaman jagung. Tujuan penelitian ini untuk mengetahui pengaruh pyrasclostrobin terhadap perkembangan penyakit bulai pada tanaman jagung serta pengaruh pyraclostrobin terhadap pertumbuhan tanaman. Metode penelitian ini menggunakan rancangan acak kelompok dengan dua faktor dan tiga ulangan. Faktor pertama menggunakan lima varietas yaitu varietas P21, BISI 2, BISI 222, Pertiwi3 dan NK 33. Faktor kedua menggunakan aplikasi pyraclostrobin dan kontrol (tanpa aplikasi pyraclostrobin). Daun tanaman jagung yang terserang bulai bewarna kuning pucat (khlorotik) memanjang sejajar tulang daun dengan batas yang jelas. Pengaplikasian pyraclostrobin berpengaruh terhadap perkembangan penyakit bulai pada lima varietas yang digunakan terbukti pada nilai intensitas yang lebih rendah pada perlakuan pemberian pyraclostrobin daripada yang tidak diberi pyraclostrobin. Pemberian pyraclostrobin tidak menunjukkan pengaruh terhadap tinggi tanaman tetapi menunjukkan pengaruh terhadap diameter batang. Pyraclostrobin dapat meningkatkan jumlah klorofil. Hal ini diduga varietas dengan pemberian pyraclostrobin dapat meningkatkan nitrogen tanaman untuk pembentukan klorofil. Pemberian pyracostrobin juga berpengaruh terhadap bobot biji jagung. Pyraclostrobin juga dapat menekan perkembangan jamur filosfer tetapi tidak dapat menekan perkembangan bakteri filosfer. Tanaman yang diperlakukan pyraclostrobin memiliki kandungan fenol yang lebih rendah daripada kontrol (tanpa pyraclostrobin). Hal ini diduga produksi fenol pada tanaman dapat diinduksi dengan serangan patogen.

UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN DAN BUAH MENGKUDU (Morinda citrifolia L.) TERHADAP Blood Disease Bacterium Husna Fikriya Baroroh; Luqman Qurata Aini; Abdul Latief Abadi
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 2 No. 2 (2014)
Publisher : Universitas Brawijaya

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ABSTRAK Penyakit darah yang disebabkan Blood Disease Bacterium (BDB) merupakan kendala serius dalam budidaya tanaman pisang di Indonesia karena dapat menyebabkan kehilangan hasil sebesar 20-100% dalam satu luasan lahan.Pemanfaatan ekstrak tanaman mengkudu khususnya bagian daun dan buah yang mengandung senyawa antibakteri menjadi alternatif pengendalian BDB yang lebih ramah lingkungan. Penelitian dilaksanakan di Laboratorium Penyakit Tumbuhan Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Brawijaya pada bulan Oktober 2013 sampai Februari 2014. Pelaksanaan penelitian meliputi isolasi dan identifikasi bakteri patogen, pembuatan ekstrak daun dan buah mengkudu, uji sensitivitas antibakteri dan uji penekanan pertumbuhan BDB pada buah pisang. Pada uji sensitivitas antibakteri perlakuan ekstrak daun dan buah mengkudu mampu menghasilkan zona hambat terhadap pertumbuhan BDB. Ekstrak buah mengkudu memiliki diameter zona hambat yang lebih besar dari ekstrak daun mengkudu. Ekstrak buah konsentrasi 90% terbaik dalam menekan pertumbuhan BDB. Pada uji penekanan pertumbuhan BDB pada buah pisang diketahui ekstrak buah dengan konsentrasi 90% dan streptomisin mampu menekan pertumbuhan BDB pada buah pisang. Â Kata kunci: Blood disease bacterium, mengkudu, uji sensitivitas antibakteri, uji penghambatan pertumbuhan BDB, pisang

PENGARUH APLIKASI Bacillus sp. DAN Pseudomonas sp. TERHADAP PERKEMBANGAN PENYAKIT BULAI YANG DISEBABKAN OLEH JAMUR PATOGEN Peronosclerospora maydis PADA TANAMAN JAGUNG Wiwik Jatnika; Abdul Latief Abadi; Luqman Qurata Aini
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 1 No. 4 (2013)
Publisher : Universitas Brawijaya

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ABSTRAK Bulai merupakan penyakit penting pada tanaman jagung yang disebabkan oleh jamur patogen Peronosclerospora maydis, dengan tingkat serangan mencapai 95%. Bacillus sp. dan Pseudomonas sp. diketahui merupakan mikroorganisme antagonis. Bakteri ini mampu menghasilkan senyawa antibiosis seperti enzim kitinase yang dapat menghidrolisis dinding sel jamur, sideropore, dan antibiotik lainnya yang dapat menghambat pertumbuhan patogen. Penelitian ini bertujuan untuk mengetahui potensial isolat Bacillus sp. dan Pseudomonas sp. dalam menekan sporulasi, perkecambahan Peronosclerospora maydis dan perkembangan penyakit bulai. Bacillus sp. dan Pseudomonas sp. mampu menekan sporulasi jamur. Tetapi, tidak dapat menekan perkecambahan jamur Peronosclerospora maydis. Bacillus sp. dan Pseudomonas sp. mampu menekan penyakit bulai.Â Tingkat penekanan tertinggi pada isolat Pseudomonas sp. UB-PF5 sebesar 50%. Bakteri terbaik yang dapat menstimulasi pertumbuhan tanaman jagung adalah isolat Pseudomonas sp. UB-PF5 dan isolat Bacillus sp. UB-ABS1. Kata kunci: Penyakit Bulai, Bacillus sp., dan Pseudomonas sp.

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