Claim Missing Document
Check
Articles

Found 9 Documents
Search
Journal : JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)

Phytochemical Screening, Determination of Total Phenolic Contents and Total Flavonoid Contents of Pod Purified Extract from Caesalpinia pulcherrima (L.) Sw. Lenny, Sovia; Surbakti, Febriyanti Br; Tarigan, Juliati Br
Journal of Chemical Natural Resources Vol. 7 No. 1 (2025): Journal of Chemical Natural Resources (JCNaR)
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v7i1.19838

Abstract

Caesalpinia pulcherrima (L.) Sw. is traditionally used in medicine and is thought to be influenced by the presence of secondary metabolites, especially phenolic compounds and flavonoids. However, identifying the phytochemical compounds of Caesalpinia pulcherrima (L.) Sw. plants, especially on the pods, are less discussed. Here, we propose research on the identification of secondary metabolite compounds of pod-purified extract from Caesalpinia pulcherrima (L.) Sw. using phytochemical screening and also the determination of total phenolic and total flavonoid content. The extraction of Caesalpinia pulcherrima (L.) Sw. pods were processed by maceration and fractionation methods to obtain the purified extract. Total phenolic contents were determined using the Folin-Ciocalteu method. Meanwhile, total flavonoid contents were determined using the AlCl3 method. The result showed that the purified extract of Caesalpinia pulcherrima (L.) Sw. pods contained phenolics, flavonoids, alkaloids, and saponins. The total phenolic and total flavonoid content in the pod purified extract were 326.038 mg GAE/g extract and 290.026 mg QE/g extract, respectively.
Isolation of Flavonoid Compounds from Guava Plant Leaves (Syzygium malaccense (L.) Merr. & Perry) Lenny, Sovia
Journal of Chemical Natural Resources Vol. 2 No. 2 (2020): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.505 KB) | DOI: 10.32734/jcnar.v2i2.9326

Abstract

Isolation of flavonoid compound from the leaves of guava (Syzygium malaccense (L.) Merr. & Perry) has been done using the extraction method. Extract guava leaves separated by using the Column Chromatography method with silica gel as the stationary phase and n-hexane: ethyl acetate (60:40) v/v as the mobile phase. The result of the isolation compound was a yellow crystal with a mass of 39 mg from fractions 325-435, a melting point of 157-159oC, and an Rf of 0.35. The compound was further identified analysis by spectroscopy analysis using Ultraviolet-Visible (UV-Vis), Fourier Transform Infra-Red Spectroscopy (FT-IR), and Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR). The spectroscopy data of the isolated compound indicated that the compound is a flavonoid named flavonol.
Isolation of Flavonoid Compounds from Suren Leaves (Toona sureni) Lenny, Sovia
Journal of Chemical Natural Resources Vol. 3 No. 1 (2021): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.539 KB) | DOI: 10.32734/jcnar.v3i1.9336

Abstract

Isolation of flavonoid compound from Suren leaves (Toona sureni) has been done using the extraction method. The suren leaves extract was separated by using the column chromatography method. The isolated compound results were yellow paste with weight 47 from fraction 55-67 and Rf = 0.44 of eluent n-hexane: ethylacetate 60:40 (v/v). The identification process was analyzed by UV-Vis, FT-IR spectroscopy, and 1H-NMR spectroscopy. The obtained result shows that the isolated compound was isoflavone.
Isolation of Flavonoid Compounds from Bangun-bangun Leaves (Plectranthus amboinicus (Lour.) Spreng.) Lenny, Sovia
Journal of Chemical Natural Resources Vol. 3 No. 1 (2021): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (610.754 KB) | DOI: 10.32734/jcnar.v3i1.9339

Abstract

Isolation of flavonoid compounds from leaves of Bangun-bangun (Plectranthus amboinicus (Lour.) Spreng.) has been conducted by maceration process with methanol solvent, added with ethyl acetate, and then partially extracted with n-hexane, acided by HCl 6% and partition extracted with chloroform. The concentrated extract of chloroform was separated using column chromatography with eluent n-hexane: ethyl acetate. The resulted tawny paste yielding was 10 mg with Rf= 0.38. The resulted compounds were characterized using UV-Vis, FT-IR, and 1H-NMR which was estimated as a flavonoid is a flavanone.
Isolation of Flavonoid Compounds from Salam (Syzygium polyantha Wight) Leaves Pasaribu, Albert; Simamora, Monojaya; Lenny, Sovia
Journal of Chemical Natural Resources Vol. 3 No. 2 (2021): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (627.543 KB) | DOI: 10.32734/jcnar.v3i2.9345

Abstract

Isolation of flavonoid compounds from Salam (Syzygium polyantha Wight) leaves has been done by maceration technique with methanol solvent. Methanol extract obtained was evaporated and dissolved with ethyl acetate. The solution was then concentrated and evaporated. The ethyl acetate fraction was dissolved with methanol and partitioned with n-hexane solvent. The methanol layer was evaporated and then tested with Benedict’s reagent and then acidified with HCl 6% while heated. It was then extracted partition with chloroform. The chloroform layer was separated using Column Chromatography with silica gel as the stationary phase and n-hexane: ethyl acetate 90:10, 80:20, 70:30, and 60:40 v/v, respectively as the mobile phase. The pure compound was paste, brownish red, mass = 8.5 mg, and Rf=0.60. It was a positive reaction with flavonoid compound reagents. The compound was further identified by using UV-Visible, FT-IR, and 1H-NMR spectroscopy. Spectroscopy data obtained indicated that the compound is a flavonoid.
Preparation of Crackers by Mixing of Cassava, Carrot, and Protein Isolated from Waste of Ketchup Factory with CaSO4 Ions Lenny, Sovia
Journal of Chemical Natural Resources Vol. 4 No. 1 (2022): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (587.774 KB) | DOI: 10.32734/jcnar.v4i1.9360

Abstract

This research is one of the diversification efforts of food substances to create economically valuable nutritious food. It has been researched the use of isolation of protein from the waste of the Ketchup Industry as the mixture of crackers with various comparisons between isolating of the waste of protein from Ketchup Industry and cassava by adding an extract of carrot about its nutrient content also. The analyzing nutrient is protein content by using the Kjeldahl method and turning to dust β – carotene using the spectrophotometer and organoleptic test about the taste of crackers using a hedonic scale. The highest protein content and β – carotene is found in the crackers in comparison (1:6:3) namely 5,38 % and 53,59 ppm. Moreover, the lowest protein content and β – carotene is found in the crackers in comparison (1:0:0) namely 2.35 % and 27.49 ppm. The most delicious, crispy, and the most people like is the crackers with comparison (1:4:2).
Isolation of Flavonoids Compounds from Kesumba Keling Leaves (Bixa orellana L.) Lenny, Sovia; Sihombing, Debynati; Marpaung, Lamek
Journal of Chemical Natural Resources Vol. 4 No. 2 (2022): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v4i2.11966

Abstract

Isolation of flavonoid compounds from leaves of Kesumba Keling (Bixa orellana L.). Extraction has been done with maceration by methanol solvent. The concentrated extract of methanol was added with ethyl acetate. The concentrated ethyl acetate extract was then dissolved with methanol and partitioned with n-hexane. The concentrated methanol extract was acidified by 6% HCl, then partition extracted with chloroform. The concentrated chloroform extract was separated by column chromatography with eluent n-hexane: ethyl acetate 90:10; 80:20; 70:30; 60:40; 50:50 (v/ v). The compounds were purified with TLC preparative yielding yellow gum weighing 29.7 mg with Rf=0.51. The compound was further identified by Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infra Red Spectroscopy (FT-IR), and Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR). Spectroscopic data show that the compound was isoflavone.
Isolation of Flavonoids Compounds from Akalifa (Acalypha wilkesiana Muell. Arc.) Plant Leaves Simangunsong, Siska NA; Lenny, Sovia; Marpaung, Lamek
Journal of Chemical Natural Resources Vol. 5 No. 1 (2023): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v5i1.11990

Abstract

Flavonoid compounds from the leaves of Akalifa (Acalypha wilkesiana Muell. Arc.) have been extracted with maceration by methanol solvent, then added with ethyl acetate and partition extracted with n-hexane. 6% HCl acidifies the concentrated methanol extract, then partition extracted with chloroform. The concentrated chloroform extract was separated by column chromatography with silica gel 40 as the stationary phase and n-hexane: ethyl acetate 90:10; 80:20; 70:30; 60:40; 50:50 (v/v) as the mobile phase. The compounds were purified with TLC preparative yielding tawny paste weighing 4 mg with Rf= 0.42 with eluent n-hexane: ethyl acetate 60:40 (v/v). The identified analysis by using Ultraviolet-Visible (UV-Vis) spectroscopy, Fourier Transform Infra Red Spectroscopy (FT-IR), and Proton Nuclear Magnetic Resonance (1H-NMR) Spectroscopy was estimated as a flavonoid is an isoflavone.
Screening of Phytochemicals and Determination of Total Phenolic and Flavonoid Contents in Different Plant Parts of Kalanchoe ceratophylla Haw. Lenny, Sovia; Karo, Alyssa Zahra Br; Manalu, Lamhot Parulian; Astuti
Journal of Chemical Natural Resources Vol. 7 No. 2 (2025): Journal of Chemical Natural Resources (JCNaR)
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jcnar.v7i2.22964

Abstract

Kalanchoe ceratophylla Haw. is a medicinal plant traditionally used to address various health disorders. Its biological activities are believed to be associated with its secondary metabolites, particularly phenolics and flavonoids. Despite its traditional use, scientific information This study assesses the phytochemical composition alongside the total phenolic and flavonoid contents in the stem and leaf preparations of Kalanchoe ceratophylla Haw. Phytochemical screening confirmed the presence of alkaloids, flavonoids, tannins, and phenols in the stem extract, while the leaf extract also contained steroids. The total phenolic content was 7.43 mg GAE/100 g extract for stem and 7.11 mg GAE/100 g extract for leaves, whereas total flavonoids amounted to 0.324 mg QE/g extract (stem) and 1.621 mg QE/g extract (leaves). These findings highlight the potential use of K. ceratophylla, especially its leaves, for developing antioxidant-based herbal medicines.