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All Journal Natural Science: Journal of Science and Technology Biocelebes Jurnal Kedokteran Universitas Palangka Raya
Musjaya M. Guli
Jurusan Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Tadulako
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Immunology & microbiology Medicine & Pharmacology Public Health

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EFEKTIVITAS MINYAK ATSIRI DAUN KEMANGI (OCIMUM TENUIFLORUM L.), DAUN JERUK PURUT (CITRUS HYSTRIX D.C.) DAUN MIMBA (AZADIRACHTA INDICA A.JUSS.), SEBAGAI REPPELENT NYAMUK AEDES AEGYPTI L. Wati, Ika Merdeka; Guli, Musjaya M.; Umrah, Umrah; Fahri, Fahri
Natural Science: Journal of Science and Technology Vol 4, No 1 (2015): Volume 4 Number 1 (March 2015)
Publisher : Univ. Tadulako

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Abstract

Penelitian Uji Efektivitas minyak atsiri daun Kemangi (Ocimum tenuiflorum), daun jeruk purut (Citrus hystrix) dan daun mimba (Azadirachta indica) sebagai reppelentAedes aegyptitelah dilaksanakan pada bulan Mei sampai Desember 2013 Laboratorium Hewan Coba di Balai Penelitian dan Pengembangan Pengendalian Penyakit Bersumber Binatang (P2B2). Penelitian didesain berdasarkan model Rancangan Acak Lengkap (6 perlakuan dan 3 ulangan) dengan waktu pengamatan selama 0 jam, 1 jam, 2 jam dan 3 jam. Desain perlakuan  P0 (Air), P1 (Soffel), P2 ( Daun Kemangi), P3 (Daun jeruk purut), P4 (Daun mimba), P5(Campuran minyak atsiri). Minyak atsiri diisolasi dengan metode destilasi uap. Nyamuk Aedes aegypti betina dipuasakan selama 24 jam, kemudian dimasukkan ke dalam kandang nyamuk yang berukuran 22 x 22 cm. Pengujian dilakukan dengan memasukkan lengan probandus yang telah diolesi pasta (perlakuan P0 sampai P5) kedalam kandang nyamuk. Parameter pengamatan meliputi Jumlah nyamuk yang hinggap dan Lama hinggap nyamuk. Hasil penelitian menunjukkan bahwa ekstrak minyak atsiri daun kemangi merupakan reppelent yang lebih efektif dibandingkan dengan ekstrak minyak atsiri daun jeruk purut dan mimba. Ekstrak minyak atsiri daun kemangi dapat menolak nyamuk dalam jumlah yang lebih banyak dan waktu hinggap lebih singkat.
Uji Serologi Terhadap Antigen Flagella Dari Isolat Salmonella typhi Di Makassar Dengan Teknik Enzyme Linked Immunosorbent Assay (ELISA Musjaya M. Guli
Biocelebes Vol. 1 No. 1 (2007)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Typhoid fever is a disease most prevalent in countries in detecting typhoid fever.  The aim of the was to discover the effectifeness of local antigens flagella to detect antibody of the typhoid fever patiens. The method applied was serological test of Elisa techniques to detect the serum titer obtained from Wahidin Sudirohusodo  General  Hospital.  The  study  was  conducted  at  the   immunology  and  Microbiology Molecular  Labrotory  of    Medicine  Faculty,  Hasanuddin  University  in  Makassar.  The  data  were analyzed by using  Wilcixon sign  test. The result of the study indicate that local antigen flagella can be used as a standard antigen in diagnosing typhoid fever.Key words: Elisa, flagella antigen (H), Salmonella typhy
Gambaran Fertilitas Masyarakat Palu Yang Melakukan Pemeriksaan Analisa Kuantitatif Sperma di Laboratorium Kesehatan Palu Musjaya M. Guli; Prita Permatasari
Biocelebes Vol. 6 No. 1 (2012)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research about decription of fertility of Palu sociaty who did analysis quantitative sperm examination at the Palu Healhty Laboratory was conducted from February – March 2011. The research aimed to know the description of Palu sociaty who did analysis quantitative sperm examination, describe the factor that be related to quality os sperm, and also to find abnormalities in sperm based on the description of fertility from quantitative analysis of sperm that mentioned. From 99 samples that eligible, factors related to sperm quality can be evaluated from macroscopic and microscopic examination. By macroscopic, the average value of cerment volume ranged from 1,6 to 5,0 ml, cement pH ranged from 7,6 to 8,0, cement liquefaction ranged from 36 to 60 minutes, and cement viscosity ranged from 0,6 to 5,0 cm. Microscopicallay, the avarage value the total number of sperm ranged from 61 to 108 million sperm/ ejakulate , sperm concentration ranged from 15,6 to 30 million /ml ejakulate , sperm motility ranged from 2-98%, sperm morphology ranged from 5 to 25%, and leucocyte sperm ranged from 0 to 2 million/ ml ejakulate. These factors are interrelateted to each other. From the description of the factors related to sperm quality was obtained subfertil conditions were 59 patients, there were 10 patients with fertile conditions, and more number of pariens with infertile conditions, there were 30 patients. This is supported by many abnormalities including Teratozoosoermia with total 33 patients, Oligoastenoteratozoospermia equal to 31 patients, Astenoteratozoospermis equal to 16 patients, Oligoteratozoospermia equal to 8 patients , and 1 patient each of Asten ozoospermia and Cryptozoospermia. Key words: Sperm, quantitative analysis of sperm, fertility.
Uji Resistensi dan Sensitivitas Bakteri Salmonella thypi Pada Orang Yang Sudah Pernah Menderita Demam Tifoid Terhadap Antibiotik Nur Indang; Musjaya M. Guli; Muhammad Alwi
Biocelebes Vol. 7 No. 1 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

This Research explores “The Resistance and Sensitivity Test of Salmonella thypi Bacteria of the People Who Had Ever Suffered from the Typhoid Fever toward the Antibiotic”, in which it was conducted on January to March 2013 at UPT Health Laboratory Palu Central Sulawesi. The objective of the research was to recognize the kind of antibiotics that is resistant and sensitive, and was to know the capacity zone of inhibition that was generated by the administering of antibiotic toward Salmonella thypi bacteria against people who had ever suffered from the typhoid fever. The researcher employed biochemical test, resistance and sensitivity test of Salmonella thypi bacteria toward the antibiotic. The findings shows that Salmonella thypi Bacteria is resistant toward the four kinds of antibiotics, they are 5.95 mm of Ampicillin, 6.9 mm of Amoxicillin, 7.9 mm of Cephalexin, and 8.1 mm of Chloramphenicol, and the Salmonella thypi Bacteria is sensitive toward four kinds of antibiotics, they are 21.35 mm of Cefatoxime, 22.6 mm of ceftazidime, 23.4 mm of Cefaperazone and 25.95 mm of Ceftriaxone. The most effective antibiotic to block the growth of Salmonella thypi bacteria is a Sefalosporin group of the third generation, they are Ceftriaxone, Cefoperazone, Ceftazidime, dan Cefatoxime. Key words: Typhoid Fever, Resistance and Sensitivity Test, Antibiotics, Salmonella thypi.
Uji Daya Hambat Ekstrak Buah Nanas (Ananas comosus L.Merr.), Salak (Salacca edulis Reinw.) dan Mangga Kweni (Mangifera odorata Griff.) terhadap Daya Hambat Staphylococcus aureus Endang Suerni; Muhammad Alwi; Musjaya M. Guli
Biocelebes Vol. 7 No. 1 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The purpose of this research was to investigate whether the extract of some fruits: pineapple (Ananas comosus L.Merr.), bark (Salacca edulis Reinw.), and Kweni mango (Mangifera odorata Griff.) has antimicrobial against the bacteria and how effective is the extract of pineapple (Ananas comosus L.Merr.), bark (Salacca edulis Reinw.), and kweni mango (Mangifera odorata Griff.). The bacteria of Staphylococcus aureus examined its growth is the bacteria purified from the common skin decease, that is, inflamed pimple. This research used Factorial RAL Design and further tested by employing Duncan Multiple Range Test (DMRT). If is found that the extract of pineapple, bark, and kweni mango can inhibit the growth of Staphylococcus aureus bacteria. The greatest inhibiting zone was the inhibiting zone produced by the extract of mango (Mangifera odorata Griff.) under the concentration of 100%. Key words: The extract of pineapple, bark, mango; inhibiting power, Staphylococcus aureus.
Uji Kandungan Plumbum (Pb) Dalam Urine Karyawan SPBU Bayaoge Kota Palu Astini Dewi Sari; Musjaya M. Guli; Miswan Miswan
Biocelebes Vol. 7 No. 1 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

A study about the test of plumbum (Pb) contents in urine Bayaogegas station employer of palu has doneat from November to December 2012. The aim of this study todetermine thecontent of plumbum(Pb) in urine Bayaogegas station Palu. Samples that were analyzed in this study were 9 urine samples with different tenure. The result showed that there were 2 urine samples of Bayaoge gas station employer has exceeded the threshold while 7 urine samples still below the threshold are allowed in the body. Key words: Plumbum, urine, SPBU (service station) Bayoge Palu
Deteksi Suspek Tuberculosis Paru Pada Pekerja Tambang Poboya Palu Sulawesi Tengah Murni Ria; Musjaya M. Guli; Muhammad Alwi
Biocelebes Vol. 7 No. 2 (2013)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Research dealing with “ Detection of Suspected Pulmonary Tuberculosis of poboya gold mine workers at Palu, Central Sulawesi” was conducted from February to April 2013. It was aimed at knowing if there was any pulmonary tuberculosis at the reseach location. This research applied a discriptive method in order to describe the research sampple based on data obtained. The research result showed that there was one worker positively getting pulmonary tuberculosis with 2.5%, and there were 39 out of 40 workers as the sample being suspects of Pulmonary tuberculosis with 97,5%. Based on the findings, it can be known that the percentace of suspected pulmonary tuberculosis of Poboya gold mine workers at Palu, Central Sulawesi, was low. Keywords: Poboya, Deteksion, Suspek, Pulmonary Tuberculosis
DETEKSI BAKTERI RESISTEN MERKURI PADA AREAL TROMOL PERTAMBANGAN EMAS KELURAHAN POBOYA PROVINSI SULAWESI TENGAH. Rahmat Pangestu; Musjaya M. Guli; Miswan Miswan
Biocelebes Vol. 8 No. 1 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Merkury is heavy metal which is toxic, So, it can damage ecosystem, ecology and environment, also give negative affect for health giving rise to disease. East Palu in Central Sulawesi there are some people that gold miners use mercury to extract gold, then discard mercury waste in the environment. Resulting in soil ant water around the village Poboya polluted. Mercury resistant bacteriahaveoperon meris ususally cotained in plasmid. This study aims to determine the type of bacteria that live in the area of gold mining village drum Poboya Central Sulawesi Province and to know the optimum HgCl2concentration on bacterial growth. Escherichia coli, Proteus mirabilis and Lactobacillus sp. can only grow at a HgCl2 concentration 7,5 g/L. Proteus vulgaris grow at HgCl2 concentration 15 g/L Enterobacter agglomerans bacteria able to grow HgCl2 concentration at 22,5 g/L. Enterobacter hafniae bacteria type and Kliebseilla sp. can grow HgCl2 concentration 30 g/L. There no bacteria type capable to grow HgCl2 concentration 37,5 g/L. Keywords: Resistant Bacteria, Mercury, Mining Gold.
IDENTIFIKASI BAKTERI FLORA NORMAL MUKOSA HIDUNG DAN SALIVA PADA PENAMBANG EMAS (TROMOL) DI KELURAHAN POBOYA KECAMATAN PALU TIMUR SULAWESI TENGAH Yuni Tiara; Muhammad Alwi; Musjaya M. Guli
Biocelebes Vol. 8 No. 1 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Research on the “Identification Of Normal Bacterial Flora of Nasal Mucosa and Salivary Gold (Tromol) Mining Village In The District Poboya Palu Central Sulawesi East” have been performed. Purpose of this study was to determine the type of bacterial that are normal flora in nasal mucosa and salivary poboya gold miners and todetermine the percentage of normal flora laboratory. Research method that was done in two stages field sampling and laboratory axamination. Sampling done by random. As for the number of samples that 30 consisting of 15 saliva and nasal mucosa 15. Result obtaind in the nasal mucosa was Enterobacter aerogenosa the proportion 18.1%, Staphyllococus aureus the proportion 36.4%, Proteus mirabilis the proportion 9.1%, Escherichia coli the proportion 9.1%, Enterobacter agglomerans the proportion 27.3%. Keywords : Identification, Normal Bacterial Flora, Nasal Mucosa, Salivary.
DETEKSI CACING PITA (Taenia solium L.) MELALUI UJI FESES PADA MASYARAKAT DESA PURWOSARI KECAMATAN TORUE KABUPATEN PARIGI MOUTONG SULAWESI TENGAH Nelky Suriawanto; Musjaya M. Guli; Miswan Miswan
Biocelebes Vol. 8 No. 1 (2014)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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This study is about incidence of pork-tapeworm (Taeniasolium L.) in Purwosari village entitled “Detection of pork-tapeworm (Taeniasolium L.) infecesof people and swine in Purwosari village, Torue, sub-district of Parigi Moutong in Central Sulawesi”. It was done over March and may, 2013. The goal of this research was to detect the tapeworm in feces of Purwosari village community. Observation was done based on kato-katz and direct slide methods. Stool sample were collect from 33 people, and from 33 swines. The result showed that pork-tapeworm was not detected from all sample. In addition, other worms i.e. Trichuristrichura, Ascarislumbricoides, Ancylostomaduodenale, grouped as Soil Transmited Helmin (STH), were detected from the sample. Based on these data, we recomend to the comunity to continue their hygiene in daily lifestyle. Keywords: Taenia solium L., Swine, Feces, Detection.
Co-Authors Afifah Islamia Andi Saifah Ardiputra, Muh. Akbar Ardiputra, Muh.Akbar Astini Dewi Sari Bambang Sardi Darmiyati Endang Suerni Eny Yuniati Fahri Fahri Ika Merdeka Wati Khairunnisa Miswan Miswan Mohammad Rivaldi Putra Pratama Muhammad Alwi Murni Ria Nelky Suriawanto Nita Nur Indang Orryani Lambui Prita Permatasari Priyandini, Nabila Rahmat Pangestu Santi Toemon , Agnes Immanuela Toemon, Agnes Immanuela Umrah Umrah Wafiq Nurfadilah Yuni Tiara Zahra, Yuyun Jannatul