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PERBANDINGAN EFEKTIVITAS ANTIFUNGI ANTARA EKSTRAK METANOL KULIT BATANG KASTURI DENGAN KETOKONAZOL 2% TERHADAP Candida albicans IN VITRO Siddik, Muhammad Baihaqi; Budiarti, Lia Yulia; Edyson, Edyson
Berkala Kedokteran Vol 12, No 2 (2016)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v12i2.1877

Abstract

Abstract: Candida albicans is the most common cause of candidacies. Ketoconazole is one of the main options treatment of candidiasis, but is reported to have experienced resistance and hepatotoxic. Extract methanol bark of kasturi contains the same active substance with, Mango that is phenolic groups, terpenoids, and saponins that are antifungal. The purpose of this study was to determine differences in the concentration of the methanol extract of the bark of kasturi with ketoconazole 2% against Candida albicans in vitro. This study was true laboratory experimental  by using randomize post test-only group designs, which consisted of 9 treatments, ie EMKBK concentration of 25%, 50% to 37.5%, 62.5%, 75%, 87.5%, 100%, ketoconazole 2% and 70% methanol (control) repetition 3 times with diffusion test. Data analysis using ANOVA and post hoc LSD test (α = 0.05). The result showed mean inhibition zone the methanol extract of the bark of kasturi against Candida albicans at a concentration of 25%, 37.5%, 50%, 62.5%, 75%, 87.5% and 100% is 7 mm; 9 mm; 10 mm; 12 mm; 16 mm; 19 mm, 22 mm and ketoconazole 2% is 15 mm and there is a significant difference between the treatment EMKBK with ketoconazole 2%. Keywords: antifungal, extract methanol bark of kasturi, ketokonazole 2%, Candida albicans  Abstrak: Candida albicans merupakan penyebab tersering kandidiasis. Ketokonazol merupakan salah satu pilihan utama untuk mengobati kandidiasis, tetapi dilaporkan telah mengalami resistensi dan bersifat hepatotoksik. Ekstrak metanol kulit batang kasturi mengandung zat aktif yang sama dengan mangga yaitu golongan fenolik, terpenoid, dan saponin yang merupakan antifungi. Tujuan penelitian ini untuk mengetahui perbedaan konsentrasi ekstrak metanol kulit batang kasturi dengan ketokonazol 2% terhadap Candida albicans In Vitro. Penelitian ini adalah eksperimental laboratoris murni dengan mengunakan randomize post test- only group designs , yang terdiri dari 9 perlakuan, yaitu EMKBK konsentrasi 25 %, 37,5 % 50 %, 62,5 %, 75 %, 87,5 %, 100 %, ketokonazol 2% dan metanol 70% (kontrol) pengulangan 3 kali dengan uji difusi. Analisis data mengunakan uji ANOVA dan uji post hoc LSD (α=0,05). Hasil penelitian didapatkan rerata zona hambat ekstrak metanol kulit batang kasturi terhadap Candida albicans pada konsentrasi 25%, 37,5%, 50%, 62,5%, 75%, 87,5% dan 100% adalah 7 mm; 9 mm; 10 mm; 12 mm;16 mm; 19 mm, 22 mm dan ketokonazol 2% adalah 15 mm dan terdapat perbedaan bermakna antara perlakuan EMKBK dengan ketokonazol 2%. Kata – kata kunci: antifungi, ekstrak metanol kulit batang kasturi, ketokonazol 2%, Candida albicans
Perbandingan Efektivitas Antibakteri antara Ekstrak Metanol Kulit Batang Kasturi dengan Ampisilin terhadap Staphylococcus aureusin Vitro Akbar, M. Rizki Valian; Budiarti, Lia Yulia; Edyson, Edyson
Berkala Kedokteran Vol 12, No 1 (2016): Februari 2016
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v12i1.350

Abstract

Abstract:Kasturi as a typical plant in South Kalimantan is one fruit that has many benefits. The barks of kasturi has proved to have benefits, especially to inhibit the activity of Staphylococcus aureus. Staphylococcus aureus is a gram positive bacteria that cause pneumonia, mastitis, and urinary tract infections. The bark of kasturi can be used in extract form. The objective of this research is to know the difference between the preparations of inhibition kasturi’s bark extract and ampicillin 30μg in inhibiting the growth of Staphylococcus aureus in vitro. This study used an experimental method consisting of 9 treatments with 3 repetitions. Treatment test in the methanol extract of the bark of kasturi 25%, 37.5%, 50%, 62.5%, 75%, 87.5% and 100%. The control group used ampicillin and 70% methanol. Bacterial test was done by using a diffusion method. The parameter measured was the amount of inhibition zone (mm) which grown on media MH. Analysis of study data used One way Annova test and Post Hoc LSD test at α=0,05. The results showed that there were significant differences between the treatment kasturi’s bark extract 25%, 50%, 62.5%, 75%, 87.5% and 100% concentration different compared to ampicillin. Meanwhile, at the concentration of 37.5% (p <0.05) was not significant. The antibacterial effectiveness was obtained from the concentration of 100%. Key words: the bark of kasturi, methanol extract, Staphylococcus aureus, inhibition zone. Abstrak: Kasturi sebagai salah satu tanaman khas yang ada di Kalimantan Selatan merupakan salah satu buah yang memiliki banyak khasiat. Kulit batang kasturi terbukti memiliki manfaat terutama dapat menghambat aktivitas Staphylococcus aureus. Staphylococcus aureus merupakan bakteri gram positif yang menyebabkan penyakit pneumonia, mastitis, dan infeksi saluran kemih. Kulit batang kasturi dapat digunakan dalam bentuk ekstrak. Tujuan penelitian ini mengetahui perbedaan daya hambat antara sediaan ekstrak kulit batang kasturi dengan ampisilin 30µg dalam menghambat pertumbuhan bakteri Staphylococcus aureus secara in vitro. Penelitian ini menggunakan metode eksperimental yang terdiri dari 9 perlakuan dengan 3 kali pengulangan. Perlakuan yang di uji adalah ekstrak metanol kulit batang kasturi 25%, 37,5%, 50%, 62,5%, 75%, 87,5% dan 100%. Dan kontrol perlakuan dengan ampisilin dan metanol 70%. Uji bakteri dilakukan menggunakan metode difusi. Parameter yang diukur adalah besaran zona hambat (mm) yang tumbuh pada media MH. Analisis data penelitian mengunakan uji One way ANNOVA dan uji Post Hoc LSD pada α=0,05. Hasil penelitian menunjukan bahwa terdapat perbedaan bermakna diantara perlakuan ekstrak kulit batang kasturi 25%, 50%, 62,5%, 75%, 87,5% dan 100% konsentrasi yang beda di bandingkan dengan ampisilin. Sedangkan pada konsentrasi 37,5% (p<0,05) tidak bermakna. Efektivitas antibakteri yang terbesar di peroleh dari konsentrasi 100%.Kata-kata kunci: kulit batang kasturi, ekstrak metanol, Staphylococcus aureus, zona hambat.
HUBUNGAN KADAR ENZIM ASETILKOLINESTERASE TERHADAP KADAR GLUKOSA PETANI YANG TERPAJAN PESTISIDA Suhartono, Eko; Edyson, Edyson; Budianto, Windy Yuliana; Sekartaji, Hapsari Lintang; Fahira, Nurul Savira; Cahyadi, Herry
Jurnal Publikasi Kesehatan Masyarakat Indonesia Vol 5, No 2 (2018): Jurnal Publikasi Kesehatan Masyarakat Indonesia
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jpkmi.v5i2.5482

Abstract

AbstrakPestisida merupakan bahan kimia yang digunakan untuk mengendalikan serangga, jamur, gulma dan lain-lain. Penggunaan pestisida yang tak terkendali menyebabkan keracunan dan berdampak pada ganguan metabolisme. Untuk membuktikan hal tersebut telah diteliti hubungan antara kadar enzim asetilkolinesterase dengan kadar glukosa darah petani yang terpajan pestisida. Rancangan penelitian ini bersifat analitik, dengan pendekatan cross sectional. Subjek penelitian adalah seluruh anggota kelompok tani Kurnia Makmur di Kecamatan Landasan Ulin Utara Banjarbaru dengan menggunakan jumlah sampel minimal sebesar 60 orang. Pemeriksaan sampel kadar enzim asetilkolinesterase dalam darah dan glukosa dalam darah dilakukan dengan bekerjasama dengan Labkesda Kabupaten Banjar dengan No. 001/LKA/C/VII/2016 s/d 60/LKA/C/VII/2016. Data dikumpulkan dari Dinas Kesehatan Banjarbaru tahun 2016. Analisis bivariat digunakan untuk menjelaskan hubungan dua variabel yaitu antara kadar kolinesterase dengan kadar glukosa darah dengan menggunakan uji Spearman dengan derajat kepercayaan 95%. Hasil penelitian didapatkan rerata kadar asetilkolinesterase pada petani berjenis kelamin laki-laki dan perempuan melebihi batas normal (L= 4,6-11,5 U/L; P=3,9-10,8 U/L). Hal ini menandakan bahwa sebanyak 63,34% petani mengalami keracunan pestisida. Sementara itu, rerata kadar glukosa darah petani masih tergolong normal (Gula darah sewaktu „T 125 mg/dL) meskipun pada petani berjenis kelamin wanita ditemukan sebanyak 5% mengalami hiperglikemia. Hasil uji Spearman didapat r =0,049 (p=0,768; p>0,05) yang berarti tidak ada hubungan yang bermakna antara kadar asetilkolinesterase dengan kadar glukosa darah petani yang terpajan pestisida.Kata-kata kunci: Pestisida, kolinesterase, glukosa, diabetesAbstractPesticides are chemicals used to control insects, fungi, weeds and others. Uncontrolled use of pesticides causes poisoning and affects metabolic disorders. To prove the above has been investigated the relationship between the levels of enzyme acetylcholineserase with blood glucose levels of farmers exposed to pesticides. The design of this study is analytic, with cross sectional approach. Research subjects were all members of Kurnia Makmur farmer group in Kecamatan Landasan Ulin Utara Banjarbaru by using minimum sample amount of 60 people. Examination of samples of cholinesterase enzyme levels in blood and glucose in blood was done in collaboration with Labkesda Kabupaten Banjar with no. 001 / LKA / C / VII / 2016 s / d 60 / LKA / C / VII / 2016. Data was collected from Banjarbaru Health Office in 2016. Bivariate analysis was used to explain the relationship between two variables, ie, between cholinesterase and blood glucose level using Spearman test with 95% confidence degree. The result showed that the level of acetylcholinesterase in male and female farmers exceeded the normal limit (L = 4.6-11.5 U / L; P = 3.9-10.8 U / L). This indicates that as many as 63.34% of farmers are poisoned by pesticides. Meanwhile, the average blood glucose level of farmers is still normal (blood sugar at „T 125 mg / dL) although the female-female farmers found as many as 5% had hyperglycemia. Spearman test result obtained r = 0.049 (p = 0.768; p> 0.05) meaning there is no significant relation between level of acetylcholinesterase with blood glucose level of farmer exposed by pesticide.Keyword: pesticide, acetylcholinesterase, glucose, diabettes
ANALISIS PENGETAHUAN, SIKAP DAN TERPAAN INFORMASI DENGAN PERILAKU HIGIENE DAN SANITASI PENJUAL SAYUR KELILING DI KABUPATEN BANJAR Khairiyati, Laily; Edyson, Edyson; Marlinae, Lenie; Ulfah, Nida
Jurnal Publikasi Kesehatan Masyarakat Indonesia Vol 4, No 3 (2017): Jurnal Publikasi Kesehatan Masyarakat Indonesia
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jpkmi.v4i3.4319

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Abstrak  Masalah kurang energi protein (KEP) sebagai salah satu masalah gizi utama yang terjadi pada anak. Didalamnya terdapat salah satu aspek yaitu higiene dan sanitasi makanan. Implikasi dari kekurangan gizi yang lama pada anak akan mengalami hambatan tumbuh kembang dan terjadinya peningkatan penyakit pada anak. Pemenuhan zat gizi masih sangat rendah di dibandingkan provinsi lain, yaitu sebesar 11,7% hal ini karena akses untuk mencapai pelosok desa dalam memenuhi kebutuhan gizi anak masih tergantung pada pasar keliling yang tidak menjamin kualitas sanitasinya. Tujuan penelitian ini adalah untuk mengetahui hubungan pengetahuan, sikap dan terpaan informasi dengan perilaku higiene dan sanitasi pada pedagang sayur keliling. Penelitian ini menggunakan desain cross sectional dengan jumlah sampel sebanyak 30 pedagang sayur keliling di Kabupaten Banjar yang diambil secara purposive sampling. Instrumen yang digunakan adalah kuesioner. Analisis data menggunakan uji chi square dengan tingkat kepercayaan 95%. Hasil penelitian menunjukkan bahwa terdapat hubungan yang signifikan antara pengetahuan dengan perilaku (nilai p = 0,044; OR = 8,33), terdapat hubungan antara sikap dengan perilaku (nilai p = 0,017; OR = 0,647), serta tidak ada hubungan antara terpaan informasi dengan perilaku (nilai p = 0,073). Berdasarkan hal tersebut dapat disimpulkan bahwa terdapat hubungan antara pengetahuan dan sikap dengan perilaku higiene dan sanitasi pada pedagang sayur keliling di Kabupaten Banjar. Kata-kata kunci: Higiene dan sanitasi, pengetahuan, sikap, terpaan informasi, perilaku  Abstract The problem of lacking protein energy (PEM) as one of the main nutritional problems that occur in children. In it there is one aspect of hygiene and food sanitation. The implications of long-term malnutrition in children will experience growth barriers and increased disease in children. The fulfillment of nutrients is still very low compared to other provinces, which is 11.7% of this is because access to reach the village corners to meet the nutritional needs of children is still dependent on the mobile market that does not guarantee the quality of sanitation. The purpose of this study is to determine the relationship of knowledge, attitude and exposure of information with hygiene and sanitation behavior on the mobile vegetable traders. This research use cross sectional design with 30 samples of vegetable sellers in Banjar Regency taken by purposive sampling. The instrument used is a questionnaire. Data analysis using chi square test with 95% confidence level. The result showed that there was a significant correlation between knowledge and behavior (p value = 0.044, OR = 8.33), there was correlation between attitude and behavior (p value = 0.017; OR = 0.647), and no relationship between exposure of information with behavior (p value = 0.073). Based on this it can be concluded that there is a relationship between knowledge and attitude with hygiene and sanitation behavior on the mobile vegetable traders in Kabupaten Banjar. Keywords: Hygiene and sanitation, knowledge, attitude, information exposure, behavior
Kinetic Parameters Analysis of Liver and Kidney Catalase Under The Influence of Cadmium and Mercury In Vitro Karantika, Ellsa Anggun; Supianur, Supianur; Edyson, Edyson; Suhartono, Eko
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.01

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Cadmium (Cd) and mercury (Hg) are toxic metals that affect human organs function, including liver and kidney. This toxic activity is because the heavy metal could induce oxidative stress and interfere antioxidant activities, including catalase (CAT). The present study was aims to evaluate the effect of Cd and Hg to liver and kidney CAT kinetic parameters in vitro. In this experiment, liver and kidney were taken from male rats (Rattus novergicus). Sample the homogenized and divided into three groups with; T0 served as control which contains liver or kidney homogenate + H2O2, T1 which contains liver or kidney homogenate + H2O2 + 0.03 mg/L CdSO4; and T2 which contains liver or kidney homogenate + H2O2 + 1 mg/L Hg. Solutions then incubated at 37ºC for 1 hour and then was prepared for CAT activity measurement. The CAT activity was measured using spectrophotometer at 240 nm. For measuring the kinetic parameters, different concentration of H2O2 were used. The kinetics parameters (Km and Vmax) were calculated using Lineweaver-Burk plot. The results shows that Cd and Hg could decrease the affinity of CAT-H2O2 complex which expressed by the higher Km and Vmax values. Also from the results, Cd has better activity to decreased the affinity of CAT-H2O2 complex than Hg. From this results, it can be concluded that Cd and Hg treatments could inhibit CAT activity in liver and kidney in vitro.
Epiphytic Weeds Control by Root Infusion Method in Oil Palm Edyson, Edyson; Murgianto, Fitrah; Ardiyanto, Adhy
PLANTA TROPIKA Vol 10, No 1 (2022)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i1.10802

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Epiphytic weeds living on oil palm trunks will complicate harvesting activities. In addition, the presence of this weed can increase the risk of accidents being hit by fruit during harvesting. The objective of this research was to obtain herbicide and its efficient concentrations to control epiphytic weeds using root infusion method. The research was arranged in a single factor completely randomized design (CRD) with five treatments and five replications. Active ingredients of herbicide that used were methyl metsulfuron at a concentration of 20%, 30%, and 40%, glyphosate 30%, and triclopyr + diesel fuel at a ratio of 1:19. All treatments except triclopyr were diluted in 100 ml water for each epiphytic weed. Weed mortality rate (%) was observed every week for one month. The results showed that a solution of 30% methyl metsulfuron herbicide in 100 ml of water and 30% glyphosate in 100 ml of water was the most optimal treatment in controlling epiphytic weeds. Cutting the entire suction root of epiphytic weeds can increase the chance of weed mortality.
Pelayanan Kesehatan di Kelompok Masyarakat Beresiko pada Pekerja TPST 3R Mulyoagung Bersatu Kabupaten Malang Supartini, Nonok; Ka’arayeno, Arie Jefry; Edyson, Edyson; Ahmadi, Kgs
Cendekia : Jurnal Pengabdian Masyarakat Vol 6 No 2 (2024): Desember
Publisher : LPPM UNIVERSITAS ISLAM KADIRI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/cendekia.v6i2.5940

Abstract

Aktivitas pekerja sampah dapat menimbulkan potensi risiko yang cukup tinggi mengingat bahaya yang dapat muncul saat kontak langsung dengan sampah. TPST 3R Mulyoagung Bersatu adalah TPST yang berada di Kecamatan Dau, Kabupaten Malang. TPST ini menerapkan pengolahan sampah masyarakat menggunakan pendekatan konsep 3R (Reduce, Reuse, Recycle). Tujuan yang ingin dicapai pada kegiatan PKM (Pemberdayaan Kemitraan Masyarakat) ini adalah memberikan edukasi kesehatan melalui pemeriksaan kesehatan dasar, penyuluhan serta pemberian terapi komplementer bekam berdasarkan kebutuhan peserta. Metode dalam menyelesaikan permasalahan mitra yakni pemeriksaan kesehatan, penyuluhan, serta pemberian terapi komplementer bekam. Hasil kegiatan PKM ini menunjukkan pekerja yang mengalami gejala klinis 13%, tingkat stres 9%, dan gangguan kualitas tidur 25% pada pre-test. Sementara hasil post-terapi keluhan gejala klinis 10%, tingkat stres 7%, gangguan kualitas tidur 20%, kepuasan terhadap terapi bekam 58%, dan rekomendasi untuk terapi bekam ke orang lain 48%. Evaluasi kepuasan pekerja terhadap terapi komplementer bekam, pekerja yang menyatakan puas 67%, rencana untuk kembali menjalani terapi 58%, dan merekomendasikan kepada orang lain 48%.
Potential Content of Palm Oil at Various Levels of Loose Fruit in Oil Palm Circle Murgianto, Fitrah; Edyson, Edyson; Ardiyanto, Adhy; Putra, Shadiar Kesuma; Prabowo, Lilik
Jurnal Agro Industri Perkebunan Vol. 9 No. 2 (2021)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v9i2.2161

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Harvesting fresh fruit bunches (FFB) is an important activity in the oil palm plantation industry. This study aimed to analyze the potential content of palm oil at the level of loose fruit that falls on the oil palm circle. Observations were made on five fresh fruit bunches with criteria 1, 3, and 5 respectively loose fruit per bunch that falls on the oil palm circle from oil palm trees that were 22, 16, 12, and 7 years old. All sample fresh fruit bunches were analyzed for potential oil to bunch ratio and oil to wet mesocarp ratio in the analytical laboratory of Bumitama Gunajaya Agro. Content of oil to wet mesocarp ratio in loose fruit 1, 3, and 5 were 48.50%, 51.98%, and 53.21% respectively. While the content of oil to bunch ratio in loose fruit 1, 3, and 5 were 24.19%, 25.52%, and 25.71% respectively. Based on variable of oil to wet mesocarp ratio, the highest potential for oil content occurs in ripe palm fruit with a level of five loose fruit   per bunch that falls on that oil palm circle. Five loose fruit per bunch on the oil palm circle can be used as an indicator for harvesters to harvest ripe fruit with optimal oil content.
Pelatihan dan Pendampingan Aplikasi Teknologi Biokonversi Sampah Organik di TPST 3R Mulyoagung Bersatu Menuju Ekonomi Hijau Supartini, Nonok; Edyson, Edyson; Ka'arayeno, Arie Jefry; Ahmadi, Kgs; Sumarno, Sumarno; Leondro, Henny
JAST : Jurnal Aplikasi Sains dan Teknologi Vol 9, No 1 (2025): EDISI JUNI 2025
Publisher : Universitas Tribhuwana Tunggadewi Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/jast.v9i1.6491

Abstract

The accumulation of organic waste at TPST 3R Mulyoagung Bersatu causes odor pollution and potential health and environmental problems. Bioconversion technology using maggots is one effort to process organic waste. Maggots are larvae of BSF (Black Soldier Fly), which can decompose organic waste for 14 days according to the larval phase in its life cycle. Bioconversion technology can convert organic waste and produce maggots as animal feed. In this community service activity, training and mentoring were carried out for partners attended by 11 participants from TPST 3R Mulyoagung Bersatu employees, so that they could apply bioconversion technology. The results of community service in applying bioconversion technology have converted organic waste and produced 60 kg of organic fertilizer from bioponds (size 2.5 x 1 m2) and as much as 30 kg of maggots.ABSTRAKPenumpukan sampah organik di TPST 3R Mulyoagung Bersatu menimbulkan masalah polusi bau, potensi gangguan kesehatan, dan masalah lingkungan.  Teknologi biokonversi dengan menggunakan maggot merupakan salah satu upaya untuk mengolah sampah organik. Maggot merupakan larva dari BSF (Black Soldier Fly) yang dapat menguraikan sampah organik selama 14 hari sesuai fase larva pada siklus hidupnya. Teknologi biokonversi selain dapat mengkonversi sampah organik juga menghasilkan maggot sebagai pakan ternak. Pada kegiatan pengabdian kepada masyarakat ini dilakukan pelatihan dan pendampingan kepada mitra yang diikuti oleh 11 orang peserta berasal dari karyawan TPST 3R Mulyoagung Bersatu agar dapat mengaplikasikan teknologi biokonversi. Hasil pengabdian kepada masyarakat berupa aplikasi teknologi biokonversi telah dapat mengkonversi sampah organik dan menghasilkan 60 kg pupuk organik dari biopond (ukuran 2,5 x 1 m2) dan sebanyak 30 kg maggot.