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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Planta Tropika Berkala Kedokteran JPKMI (Jurnal Publikasi Kesehatan Masyarakat Indonesia) Jurnal Agro Industri Perkebunan JAST : Jurnal Aplikasi Sains dan Teknologi Cendekia : Jurnal Pengabdian Masyarakat Homeostasis: Jurnal Mahasiswa Pendidikan Dokter
Edyson Edyson, Edyson
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Kinetic Parameters Analysis of Liver and Kidney Catalase Under The Influence of Cadmium and Mercury In Vitro Karantika, Ellsa Anggun; Supianur, Supianur; Edyson, Edyson; Suhartono, Eko
Journal of Tropical Life Science Vol 6, No 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.01

Abstract

Cadmium (Cd) and mercury (Hg) are toxic metals that affect human organs function, including liver and kidney. This toxic activity is because the heavy metal could induce oxidative stress and interfere antioxidant activities, including catalase (CAT). The present study was aims to evaluate the effect of Cd and Hg to liver and kidney CAT kinetic parameters in vitro. In this experiment, liver and kidney were taken from male rats (Rattus novergicus). Sample the homogenized and divided into three groups with; T0 served as control which contains liver or kidney homogenate + H2O2, T1 which contains liver or kidney homogenate + H2O2 + 0.03 mg/L CdSO4; and T2 which contains liver or kidney homogenate + H2O2 + 1 mg/L Hg. Solutions then incubated at 37ºC for 1 hour and then was prepared for CAT activity measurement. The CAT activity was measured using spectrophotometer at 240 nm. For measuring the kinetic parameters, different concentration of H2O2 were used. The kinetics parameters (Km and Vmax) were calculated using Lineweaver-Burk plot. The results shows that Cd and Hg could decrease the affinity of CAT-H2O2 complex which expressed by the higher Km and Vmax values. Also from the results, Cd has better activity to decreased the affinity of CAT-H2O2 complex than Hg. From this results, it can be concluded that Cd and Hg treatments could inhibit CAT activity in liver and kidney in vitro.
Co-Authors Ardiyanto, Adhy Cahyadi, Herry Eka Yudha Rahman Eko Suhartono Fahira, Nurul Savira Husnul Khatimah Ka'arayeno, Arie Jefry Karantika, Ellsa Anggun Kgs Ahmadi Laily Khairiyati Lenie Marlinae Leondro, Henny Lia Yulia Budiarti M. Rizki Valian Akbar, M. Rizki Valian Maulida, Maulida Murgianto, Fitrah Nida Ulfah, Nida Nonok Supartini Prabowo, Lilik Putra, Shadiar Kesuma Sekartaji, Hapsari Lintang Siddik, Muhammad Baihaqi Sumarno . Supianur, Supianur Windy Yuliana Budianto, Windy Yuliana