Asri Pirade Paserang
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Tadulako

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Journal : Natural Science: Journal of Science and Technology

INDUKSI KALUS TANAMAN KENTANG DOMBU (SOLANUM TUBEROSUM L.) SECARA IN VITRO DENGAN PEMBERIAN ZPT 2,4-D (DICHLOROPHENOXY ACETID ACID) Idris, Siti RLR; Paserang, Asri Pirade
Natural Science: Journal of Science and Technology Vol 8, No 2 (2019): Volume 8 Number 2 (August 2019)
Publisher : Univ. Tadulako

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Abstract

This research was aimed to determine the effect of the PGR 2.4-D (Dichlorophenoxy Acetid Acid) in various concentrations on induceing callus of Dombu potato (Solanum tuberosum L.). This research was performed based on Completely Randomized Design (RAL) with 6 treatments and 3 times repetation, so there were 18 experiment units. Each experiment used 3 explants so as there were 54 explants. The combination of concentration of the tested PGR in the culture media were T1 = MS0+ 2.4-D 0 ppm (control), T2 = MS0+ 2.4-D 0.5 ppm, T3 = MS0+ 2.4-D 1 ppm, T4= MS0+ 2.4-D 1.5 ppm, T5 = MS0+ 2.4-D 2 ppm and T6 = MS0+ 2.4-D 2.5 ppm. The results showed that callus induction was appeared in the concentration of 1.0, 1.5, 2.0, and 2.5 ppm. Callus was generally formed on the Day-8 after plantation. Callus color was mostly greenish transparent, callus texture was mostly crumb type, and callus formation percentage was almost 100%. The best media for inducing the callus was in treatment T4 (1.5 ppm 2.4-D), it referred to the formed callus biomass
JENIS - JENIS LAMUN (SEAGRASS) DI KECAMATAN TINANGKUNG, BANGGAI KEPULAUAN, SULAWESI TENGAH Pranata, Adhi; Suwastika, I Nengah; Paserang, Asri Pirade
Natural Science: Journal of Science and Technology Vol 7, No 3 (2018): Volume 7 Number 3 (December 2018)
Publisher : Univ. Tadulako

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Penelitian ini bertujuan untuk mengetahui jenis-jenis lamun (seagrass) di Kecamatan Tinangkung, Banggai Kepulauan, Sulawesi Tengah. Pengambilan sampel lamun (seagrass) dilakukan dengan metode jelajah pada saat air menjelang surut. Hasil identifikasi menunjukkan bahwa Lamun di lokasi penelitian terdiri dari 9 jenis yakni: Cymodocea rotundata dan Cymodcea serulata, Enhalus acoroides, Halodule pinifolia, Halodule uninervis, Halophila ovalis, Syringodium isoetifolium, Thalasia hemprichi, dan Thalasodendron ciliatum. Dari 9 spesies yang saya dapatkan ada beberapa jenis lamun yang masih jarang ditemukan di tempat lain, seperti Halophila Ovalis dan Thalasodendron Ciliatum
INDUKSI KALUS KENTANG ASAL DESA DOMBU (SOLANUM TUBEROSUM L.) DENGAN ZPT INDOLE-3-ACETIC ACID (IAA) Zulraufianti, Lyly; Paserang, Asri Pirade
Natural Science: Journal of Science and Technology Vol 8, No 2 (2019): Volume 8 Number 2 (August 2019)
Publisher : Univ. Tadulako

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Abstract

The research of callus induction in potato (S. tuberosumĀ  L.) from Dombu Village with indole-3-acetic-acid (IAA) was conducted from Januari to April 2019 in Laboratory of Tissue Culture, Biology Department, Faculty of Mathematics and Natural Science, Tadulako University. The research was aimed to determine of concentration IAA of the best to induce callus. This study was designed based on Completely Randomized Design (CRD) with 5 treatments, 3 replications and 3 explants on each unit. The treatments were P1= MS + 0 ppm, P2= MS + 0.5 ppm, P3= MS + 1.0 ppm, P4= MS + 2.0 ppm, P5= MS + 3.0 ppm. The results showed thatĀ  the best and the efficient treatments for induction of callus treatment was P4= MS + 2.0 ppm with to induce callus up to 100%, the callus colour was yellow to brown and intermediate texture.