MEITY A. TULALO, MEITY A.
Balai Penelitian Tanaman Palma Jalan Raya Mapanget, Manado 95001

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KERAGAMAN FENOTIPE DAN GENETIK TIGA VARIETAS KELAPA GENJAH KOPYOR ASAL PATI JAWA TENGAH MASKROMO, ISMAIL; TENDA, ELSJE T.; TULALO, MEITY A.; NOVARIANTO, HENGKY; SUKMA, DEWI; SUKENDAH, SUKENDAH; SUDARSONO, SUDARSONO
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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Abstract

ABSTRAKKelapa Genjah kopyor asal Pati, Jawa Tengah merupakankekayaaan hayati asli Indonesia dengan nilai ekonomi tinggi. Informasikeragaman genetik kelapa kopyor masih terbatas. Data keragamanmorfologi dan genetik diperlukan dalam program pemuliaan kelapakopyor. Penelitian ini mempelajari keragaman tiga varietas kelapa genjahkopyor asal Pati yang telah dilepas berdasarkan karakter morfologi,kuantitas endosperma, dan keragaman alel marka SSR. Penelitiandilakukan di Pati dan di Laboratorium Plant Molecular Biology,Departemen Agronomi dan Hortikultura, IPB. Evaluasi dilakukan terhadaptiga populasi kelapa Genjah kopyor (hijau, coklat, dan kuning) dengan 30tanaman sampel untuk setiap populasi. Rataan data morfologi digunakanuntuk menyusun dendogram. Kuantitas endosperma diamati pada satubuah kelapa kopyor per tanaman yang dievaluasi. Karakteristikendosperma dikelompokkan sesuai kategori yang telah ditetapkan. Untuksetiap populasi, analisis marka dengan lima pasang primer SSR dilakukanpada 10 tanaman sampel. Data yang didapat digunakan untuk menentukankeragaman genetik kelapa Genjah kopyor asal Pati. Hasil pengamatanmenunjukkan keragaman morfologis dan alel SSR antar tanaman dalamvarietasnya (keragaman intra-varietas) rendah. Sebaliknya, keragamanmorfologis dan alel SSR antar varietasnya tinggi. Kuantitas endospermakelapa Genjah kopyor asal Pati bervariasi antara skor 1–6. Keragamangenetik yang rendah dalam varietas dan tinggi antar ketiga varietas (coklat,hijau, dan kuning) memperkuat pelepasan ketiganya sebagai varietas lokal.Selain itu, keragaman genetik antar tanaman dalam varietas yang rendahmendukung penggunaan ketiga varietas lokal sebagai tetua dalam programperakitan varietas kelapa kopyor unggul baru. Tetua yang dipilih dapatdiseleksi intra-varietas berdasarkan persentase buah kopyor per tandandan skor kuantitas endosperma yang tinggi.Kata kunci: Keragaman morfologis, keragaman intra dan antar varietas,kuantitas endospermaABSTRACTKopyor dwarf coconuts are mutants from Pati, Central Java havinghigh economic values. However, morphological and genetic diversities ofthis coconut were still limited. Morphological and genetic diversity dataare needed for breeding program. The research objectives were to evaluateintra and inter-specific diversity based on morphology, endospermquantity, and SSR alleles. Field evaluations were conducted in Pati whilelaboratory activities were at Plant Molecular Biology Laboratory,Department of Agronomy and Horticulture, IPB. Three populations ofkopyor dwarf varieties (brown, green, and yellow) were evaluated. Thirtytrees were sampled for each population. The average of morphologicaldata were used to construct cluster analysis. Endosperm quantity wasscored (0 – 9) based on a single nut sample. Ten palms were analyzedusing five SSR loci for each population and used to determine geneticdiversity of populations. Results of observations indicated intra-varietymorphological and SSR allele variations among kopyor dwarf was low.However, inter-variety variations were high. The endosperm quantityscores among kopyor dwarf coconut ranged from 1–6. The low intra-variety and high inter-variety variations among the three kopyor dwarfcoconut supported their release as different local varieties. Moreover, thelow intra-variety phenotypic and genotypic diversities among kopyorbrown, green, and yellow dwarf coconut support their use as parents fornew and superior kopyor coconut variety development in the future. Forsuch purpose, however, it is necessary to conduct intra-variety selection toidentify desirable parents based on high kopyor fruit percentage per bunchand for high kopyor endosperm quantity.Key words: Morphological diversity, intra and inter variety diversities,quantity, endosperm
Lampanah Local Tall-A High Yielding Variety for Replanting Coconut in Tsunami Affected Aceh Province Area Novarianto, Hengky; Maskromo, Ismail; Tulalo, Meity A.; Kumaunang, Jeanette; Mawardi, Sukmawati; Sulistyowati, Emy
International Coconut Community Journal Vol 33 No 2 (2017): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.32 KB) | DOI: 10.37833/cord.v33i2.47

Abstract

Aceh province is one of major coconut producing areas in Indonesia. In 2013 coconut areas in Aceh province was 107,654 ha in which total production of copra was equivalent to 63,743 tons. The entire coconut area in Aceh belong to smallholders. The coconut production in this area began to decrease in 2005 by an average of 9 percent per year, which was mainly caused by the tsunami. This had resulted in damage to many crops. Efforts to increase production and productivity of coconut plantation in Aceh needed rejuvenation of the old or unproductive coconut palms with the use of seeds of improved quality. Based on the results of the research conducted during 2014-2016, it is known that the origin of the Lampanah tall is passed from generation to generation and is a native of coconut in Aceh Besar Regent, Aceh Province. The morphology of Lampanah tall coconut comprising of the crown of leaves, stems, flowers and fruits in general is quite uniform. Of the data on the 23 quantitative characters, all have a value of coefficient of variance below 20%, except the character of 11 leaf scars obtained 23.29%. In the case of polar girth, Lampanah tall coconut is generally greater than the equatorial girth, fruit shape classified as round, egg-shaped and elliptic. The seednut of Lampanah tall generally round shape. The Lampanah tall coconut has the number of bunches at 13.35/palm and the number of fruit at 9.25 fruits/bunch or an average of 138 fruits/palm/ year. Fresh coconut meat weight is 449 g/nut or approximately 224 g copra/nut and the potential for copra production was 30.97 kg/palm/year or 3.80 tons of copra/ha/year. The fat content of Lampanah tall coconut was 66.40%, the water content of copra approximately 3.42% and protein at approximately 6.81%. Total saturated fatty acids was at 94.27%, medium chain saturated fatty acids at 67.21% and lauric acid content at 46.50%. The seed source of Lampanah tall coconut has been a selection of as much as 72 Selected Mother Palms (MAS) from a total of 407 palms of the High Yielding Block. The selected population is characterised with traits like copra yield more than 2.0 ton/ha/year, free from attack pests and diseases, number of bunch more than 12 per year, total nuts more than 7/bunch and weight of meat more than 400 g/nut. The MAS of Lampanah tall whole coconut has been marked using GPS. The Aceh government will build a minimum of 5 ha Coconut tall Seed Gardens on government land, as the development of source of Lampanah tall seednuts. Based on the above results, it can be concluded that the Lampanah tall coconut with plenty of nuts, at an average of 138 nuts per palm could be released as one of the national high yielding coconut.
Preliminary Investigation of The Potential of Coconut Sugar Production Using Dwarf Varieties Novarianto, Hengky; Tulalo, Meity A.; Mawardi, Sukmawati
International Coconut Community Journal Vol 37 (2021): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v37i.444

Abstract

Coconut sugar is one of the high economic value products. The production of coconut sugar In Indonesia is about 300,000 tonnes/year. During the last few years, the supply of raw materials for the coconut sugar product has diminished due to stagnant production caused by the lack of coconut climbers collecting coconut sap. The scarcity of labor is caused by several factors, especially young tappers who are less interested in climbing tall coconut palms. To solve this problem, it is necessary to evaluate the potency of dwarf coconuts, with short trunk and fast fruiting. The objective of this study was the evaluation and selection of Dwarf coconut varieties that have high yield potential as a producer of sap and coconut sugar. The research was conducted in North Sulawesi Province by evaluating 9 Dwarf coconut varieties. The results of research on the production of coconut sap and sugar from 9 Dwarf coconut varieties with different plant ages, gave mixed results among coconut varieties. The length of tapping sap varied between 31.42 - 43.21 days/bunch, the volume of sap varied between 1.1 - 3.3 liters/tree/day, the pH of sap ranged between 6.49 - 7.86 and the Brix value varied between 14.01 - 17.64. The most important traits such as the sap yield and sugar production also varied from 14.54 to 18.95% among varieties, and between 0.16 to 0.42 kg/tree/day respectively. The Dwarf coconut varieties with the highest potential yield of sap and sugar are the Waingapu Red Dwarf (WRD), Salak Green Dwarf (SGD), and Nias Yellow Dwarf (NYD), with the potential sugar yield of 2.09 tons, 1.64 tons, and 1.56 tons/month/ha respectively. It is hoped that these Dwarf coconut varieties would be attractive for young tapers and could be introduced to farmers in several Provinces for tapping purposes for the production of coconut sugar.
Hengniu: Fast Bearing and High Yielding Coconut Varieties Novarianto, Hengky; Tulalo, Meity A.; Mawardi, Sukmawati; Mahayu, Weda Makarti
International Coconut Community Journal Vol 40 (2024): CORD
Publisher : International Coconut Community

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37833/cord.v40i.477

Abstract

Benefits of coconut varieties that stakeholders want are early bearing, high yielding, short stems, and slow growth for height. Most local tall coconut palms in Indonesia have stem height above 20 m, making it increasingly difficult to climb to harvest fruit or tapping sap thereby making the harvesting cost high. To breed varieties of desired characters, an evaluation trial was initiated in 2014 with BYD x MTT-S4, RAD x MTT-S4, and KHINA-1 as a control. The crossing was done in 2012 and seedlings planted in January 2014 at Mapanget Experimental Garden, Indonesian Palma Crops Research Institute, North Sulawesi. The study used a Randomized Block Design (RBD) of three types of coconut hybrids, four replications with a plot size of 16 trees. Morphological observations were carried out on stem, crown and leaf characters, inflorescences and flowers, nut production, fruit components, copra, oil content, and fatty acid composition. The results of ANOVA analysis and statistical tests obtained those vegetative characters, such as stem circumference and number of leaf, generative characters namely the number of bunches, first flowering, and fruit production of these three crosses at the age of 4 years after planting, did not show a significant difference. The first initial flowering was in the coconut hybrid of RBD x MTT-S4, which is 26 months, followed by BYD x MTT-S4 in 32 months and KHINA-1 in 36 months after planting. The results of the analysis of fruit and copra production at the age of 5 years showed a significant increase between hybrid coconuts. The highest to lowest fruit production was obtained in BYD x MTT-S4 hybrid coconut, RBD x MTT-S4, KHINA-1, which were 64 nuts, 44 nuts and 26 nuts /palm respectively, or estimated copra production was 2.26 tons, 1.45 tons and 0.88 tons copra/ha. At 6 years old the harvest of fruits from the three hybrid coconuts is obtained sequentially 118 nuts, 99 nuts and 94 nuts/palm. While estimation of copra yield per hectare is found the highest in BYD x MTT-S4 hybrid coconut is 3.86 ton/ha/year and this yield differently significant compare with RBD x MTT-S4 is found 3.04 ton/ha/year, and control hybrid of KHINA-1, which is about 2.74 ton/ha/year. Based on the production potential, hence the estimated optimum production when aged over 10 years can reach more than 5 tons/ha/year. The hybrid coconut variety BYD x MTT-S4 is released in October 2019 under the name HENGNIU.