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Anak Agung Istri Ratnadewi
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, University Of Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Education Electrical & Electronics Engineering Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Mechanical Engineering Physics Other

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Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production Ratnadewi, Anak Agung Istri; Kurniawan, Andika Ade; Handayani, Wuryanti
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer  have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among  the other XOS   X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm). Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase
Transformation of Plasmid pET Endo-1,4-β-xilanase from E. coli TOP10 to E. coli BL21 Santoso, Agung Budi; Kurniawati, Eka Yuni; Ratnadewi, Anak Agung Istri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

In this research we successfully transmited Plasmid pET- Endo from E.coli TOP10 to E.coli BL21. Plasmid pET Endo is recombinant plasmid base on pET-30a(+)  inserted with gene of endo-1,4-β-xilanase isolated from bacillus subtilis sp which is originally living in termite abdomen. First step is isolation of plasmid pET-Endo from E.coli TOP10 with alkaline lyses. Denaturation and renaturation of DNA occurred then separated with centrifugation. Plasmid pET-Endo is smaller than Chromosome DNA. Agarose gel electrophoresis confirmed that Plasmid pET-Endo has isolated. Electrogram of pET-Endo show the band in 6022 bp compare to empty plasmid pET-30a(+) 5422 bp. E.Coli BL21 got pretreatment with  CaCl2 solution to make cell competent for transformation. Isolated pET-Endo inserted to E.coli BL21 with heat shock method. Resistance test with antibiotic has done to know the result of transformation. E.coli BL21 contained plasmid pET-Endo will survive in kanamycin agar media. Colony of E.coli BL21 then cultured in liquid media and examinee it’s growth phase. IPTG as gene inducer given after 2,5 hour of inoculation. Crude enzyme tested for xilanase activity and well proved. Isolation of plasmid pET-Endo then running in Agarose gel electrophoresis also confirm good result of transformation.
Transformation of Plasmid pET Endo-1,4-β-xilanase from E. coli TOP10 to E. coli BL21 Agung Budi Santoso; Eka Yuni Kurniawati; Anak Agung Istri Ratnadewi
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

In this research we successfully transmited Plasmid pET- Endo from E.coli TOP10 to E.coli BL21. Plasmid pET Endo is recombinant plasmid base on pET-30a(+)  inserted with gene of endo-1,4-β-xilanase isolated from bacillus subtilis sp which is originally living in termite abdomen. First step is isolation of plasmid pET-Endo from E.coli TOP10 with alkaline lyses. Denaturation and renaturation of DNA occurred then separated with centrifugation. Plasmid pET-Endo is smaller than Chromosome DNA. Agarose gel electrophoresis confirmed that Plasmid pET-Endo has isolated. Electrogram of pET-Endo show the band in 6022 bp compare to empty plasmid pET-30a(+) 5422 bp. E.Coli BL21 got pretreatment with  CaCl2 solution to make cell competent for transformation. Isolated pET-Endo inserted to E.coli BL21 with heat shock method. Resistance test with antibiotic has done to know the result of transformation. E.coli BL21 contained plasmid pET-Endo will survive in kanamycin agar media. Colony of E.coli BL21 then cultured in liquid media and examinee it’s growth phase. IPTG as gene inducer given after 2,5 hour of inoculation. Crude enzyme tested for xilanase activity and well proved. Isolation of plasmid pET-Endo then running in Agarose gel electrophoresis also confirm good result of transformation.
Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production Anak Agung Istri Ratnadewi; Andika Ade Kurniawan; Wuryanti Handayani
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer  have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among  the other XOS   X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm). Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase
OPTIMASI KONSENTRASI SUBSTRAT XILAN AMPAS TAHU TERHADAP ENDO-Β-1,4-D-XYLANASE UNTUK MEMPRODUKSI XILOOLIGOSAKARIDA Anak Agung Istri Ratnadewi; Wuryanti Handayani; Siti Nur Avida
Jurnal Kimia Riset Vol. 1 No. 2 (2016): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.779 KB) | DOI: 10.20473/jkr.v1i2.3084

Abstract

AbstrakAmpas tahu merupakan limbah samping dari proses pengolahan tahu dan susu kedelai. Ampas tahu berpotensi sebagai sumber xilan. Xilan digunakan sebagai substrat endo-β-1,4-D-xilanase untuk menghasilkan xilooligosakarida. Penelitian ini digunakan xilan ampas yang telah dihilangkan lemak dan protein tanpa penghilangan lignin (X1nD). Xilan ampas tahu tanpa penghilangan lemak dan protein tetapi dilakukan penghilangan lignin (X2D). Enzim yang digunakan adalah endo-β-1,4-D-xilanase dari isolat Bacillus sp. asal abdomen rayap. Optimasi variasi konsentrasi substrat bertujuan untuk mengetahui konsentrasi optimum dalam menghasilkan xilooligosakarida. Produk hidrolisis yang dihasilkan dianalisis menggunakan metode Miller untuk mengetahui total gula pereduksi. Produk hidrolisis konsentrasi optimum dianalisis menggunakan Kromatografi Lapis Tipis (KLT) untuk mengetahui komponen penyusun xilooligosakarida. Substrat X1nD dan X2D optimum pada konsentrasi 6% dan 5% dengan total gula pereduksi sebesar 0,196 mg/ml dan 0,211 mg/ml. Komponen penyusun xilooligosakarida ampas tahu berupa xilotriosa (X3), xilotetraosa (X4), dan xilopentaosa (X5).Kata Kunci: Ampas tahu, endo-β-1,4-D-xilanase, xilan, xilooligosakarida. AbstractOkara is a waste byproduct of the processing of tofu and soy milk. Okara potential as a source of xylan. Xylan is used as the substrate endo-1,4-β-D-xylanase to produce xyloologosaccharide. This study used okara xylan had eliminated fat and protein without removal of lignin (X1nD). Okara xylan out without the removal of fat and protein but do removal of lignin (X2D). The enzyme used is endo-1,4-β-D-xylanase of isolates of Bacillus sp. From abdominal termites. Optimization of substrate concentration variation aims to determine the optimum concentration in generating xyloologosaccharide. Hydrolysis products were analyzed using Miller method to determine total reducing sugars. The optimum concentration of hydrolysis products were analyzed using Thin Layer Chromatography (TLC) to determine the components of xyloologosaccharide. X1nD and X2D optimum substrate at a concentration of 6% and 5% to the total reducing sugars of 0.196 mg/ml and 0.211 mg/ml. Xyloologosaccharide of okara components of the pulp out the form xylotriose (X3), xylotetraose (X4), and xylopentaose (X5).Keywords: Okara, endo-1,4-β-D-xylanase, xylan, xyloologosaccharide
The Analysis of Pb and Cu levels in Canned fish and Sauces for the Storage Time Hefinda Erfiandika; Asnawati Asnawati; Anak Agung Istri Ratnadewi
Jurnal ILMU DASAR Vol 15 No 2 (2014)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.887 KB) | DOI: 10.19184/jid.v15i2.835

Abstract

Lead (Pb) is pollutant that found in canned foods which derived from the soldering between the can and the lead. Copper (Cu) is one of material which one tin packaging can be oxidized and dissolved in acidic foods. Pb and Cu are not dangerous at lowest but it can cause botulism in gross. The storage time can affect the solubility of the metals. The purpose of the research is to know levels metal Pb and Cu in fishes and sauces canned and compared with limit BPOM. The limit of BPOM in canned fish for metal Pb is 0,3 ppm and for metal Cu is 5 ppm. The steps of the method are optimization the method of destruction and the measurement using Atomic Absorption Spectrofometry (SSA). The result shows that the storage time  give effect to the greater of metal Pb and Cu in fish and sauce.The content of metal Pb in all sample exceeded the limit of BPOM. The content of metal Cu in sample A does not exceed in fish and sauce. The first month of sample B does not exceed, but the sixth month up to the twenty fourth month exceed the limit of BPOM in fish and sauce. The precision in all the measurement have on average  <2 %, it  shows that all the measurements are good repetition. Keywords: Lead , Copper, Atomic Absorption  Spectrofometry, fish, Sauce 
Pengaruh Konsentrasi Asam Sulfat dalam Proses Hidrolisis Selulosa dari Kulit Buah Naga Merah (Hylocereus costaricensis) untuk Produksi Bioetanol Rosa Safitri; Indras Dwi Anggita; Firda Marta Safitri; Anak Agung Istri Ratnadewi
Prosiding Industrial Research Workshop and National Seminar Vol 9 (2018): Industrial Research Workshop and National Seminar
Publisher : Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1544.606 KB) | DOI: 10.35313/irwns.v9i0.1082

Abstract

Kulit buah naga merah (Hylocereus costaricensis) merupakan limbah yang mengandung karbohidrat sebanyak6,5% dari beratnya. Kulit buah naga merah dapat digunakan sebagai sumber energi (bioetanol) karenamengandung karbohidrat, yaitu selulosa. Proses pembuatan bioetanol dilakukan melalui tiga tahap, yaitudelignifikasi, hidrolisis dan fermentasi. Hasil delignifikasi kulit buah naga merah diuji kualitatif dengan FTIRdan menunjukkan bahwa hasil delignifikasi berupa selulosa. Selulosa yang dihasilkan sebanyak 20%. Selulosadikonversi menjadi glukosa menggunakan metode hidrolisis dengan asam sulfat dan dilanjutkan produksibioetanol melalui fermentasi menggunakan Saccharomyces cereviciae. Parameter yang digunakan dalampenelitian ini yaitu konsentrasi asam sulfat yang digunakan selama proses hidrolisis (0,5;1,0;1,5 dan 2,0 M)untuk menghasilkan konsentrasi glukosa yang optimum. Uji kualitatif hasil hidrolisis dengan menggunakananalisis FTIR menunjukkan adanya glukosa dalam sampel. Sementara hasil uji kuantitatifnya menunjukkanbahwa pada konsentrasi asam sulfat 2,0 M menghasilkan glukosa paling besar yaitu 0,05 g/mL. Etanol yangdihasilkan dari proses fermentasi diuji kualitatif dan menunjukkan bahwa positif menghasilkan etanol yangdapat dijadikan sebagai sumber bioetanol. Penelitian ini dilakukan dengan tujuan untuk memperoleh sumberenergi alternatif, sehingga dapat mengatasi adanya kelangkaan energi.
Inovasi Penambahan Prebiotik Xilooligosakarida Dan Peningkatan Pemasaran Pada Produk Usaha Kecil Penghasil Kue Pia di Kota Jember Anak Agung Istri Ratnadewi; Wuryanti Handayani; Sudarko Sudarko; Nyoman Gede Krishnabudi
Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) Perguruan Tinggi Mengabdi: Berkarya dan Berinovasi Untuk Membangun Masyarakat Semakin Tangguh di Mas
Publisher : Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS)

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Abstract

Program Pengabdian Berbasis Penelitian (PPBP) ini untuk memberdayakan ekonomi usaha pembuatan kue khas Jember. UKM yang dilibatkan adalah UKM produk kue Pia merk “Syam”, usaha ini tidak jauh dari Universitas Jember berkisar 7 Km. Merk kue Pia “Syam” merupakan salah satu usaha kue home industri yang melibatkan ibu-ibu rumah tangga di sekitarnya. Pemasaran masih disekitar toko-toko kue disekitar kota Jember. Usaha kue ini meskipun sudah berjalan lama di mulai usaha sejak 2006, namun masih relatif tradisional sehingga memerlukan pengembangan untuk dapat bersaing untuk menghasilkan produk kue yang berkualitas dan dapat bersaing di pasaran. Permasalahan yang muncul adalah (1) bagaimana meningkatkan kualitas produk sehingga mampu menjadi produk andalan yang dicari pengguna, dengan produk kue yang mempunyai nilai qisi dan kualitas yang terjamin, (2) bagaimana meningkatkan pemasaran produk kue Pia. Berdasarkan permasalahan tersebut maka solusi yang dapat diterapkan adalah membantu mitra bagaimana kualitas produk ditingkatkan menggunakan konsep sains dan teknologi dari praktisi perguruan tinggi. Dalam kegiatan pengabdian ini Kelompok KeRis Enzim akan membantu mengatasi masalah pertama dengan meningkatkan kualitas kue dengan memberikan inovasi pada produk kue dengan penambahan bahan prebiotik xilooligosakarida (XOS) melalui teknologi enzimatis. Kandungan prebiotik XOS diperoleh dari penambahan enzim xilanase pada adonan kulit kue Pia yang berbasis bahan tepung. Secara in-situ enzim ini akan menghidrolisis substrat pada adonat kulit kue Pia menghasilkan prebiotik XOS yang sangat bermanfaat untuk kesehatan. Enzim xilanase merupakan hasil penelitian Kelompok KeRis Enzim telah diuji stabilitas dan kemampuannya menghasilkan produk prebiotik XOS dan enzim ini telah mempunyai No paten IDP 000075875. Prebiotik merupakan oligosakarida yang tidak terdigesti dan menguntungkan untuk pertumbuhan bakteri yang baik dalam sistem pencernaan, dapat membantu penyerapan nutrisi dan melancarkan metabolism kolesterol dalam tubuh. Dengan kandungan prebiotik XOS, produk kue yang dihasilkan akan mempunyai nilai lebih dibanding produk lain, sehingga akan mempunyai ciri khas yang berbeda dengan produk yang sudah ada dipasaran. Pada permasalahan kedua Kelompok Keris Enzim membantu meningkatkan pemasaran dengan mengenalkan metode pemasaran modern berbasis IT sehingga produk mudah dikenal dan mudah dipesan. Bentuk kegiatan yang akan kami lakukan melalui pendampingan intensif pembuatan produk dengan penambahan bahan prebiotik XOS dengan teknik enzimatis. Kegiatan pemasaran usaha ini masih konvensional yaitu dengan menawarkan produk kepada kenalan dan orang terdekat melalui Whatsapp dan Facebook pribadi pemilik usaha saja. Hal ini membuat cakupan pemasaran dan produktivitas usaha rumah tangga Kue Pia “Syam” Jember kurang dapat berkembang dan terbilang belum dapat bersaing di pasar. Perlu adanya pelatihan dan pendampingan untuk membantu kegiatan promosi, pemasaran serta branding produk kue pia dengan memanfaatkan fasilitas akun sosial media dan aplikasi lainnya untuk dapat menjawab peluang pasar yang lebih luas secara online seperti misalnya terafiliasi dengan jasa layan antar produk semacam Gofood, Grabfood atau jasa layan antar lokal sejenis di area Jember dan sekitarnya. Selain itu untuk mengembangkan sayap agar permintaan tidak sebatas di Jember saja, maka perlu juga memasukkan unsur pemasaran digital atau ecommerce semacam Shopee, Bukalapak, Tokopedia dan lain-lain.Kata kunci : UKM Syam, Kue Pia, prebiotik, xilooligosakarida
Produksi Xilosa dari Xilan Limbah Ampas Singkong Menggunakan Bacillus subtilis, Aureobasidium pullulans, dan Penicillium janczewskii Arianti, Fefpi Nur Afnifitri Wias; Ratnadewi, Anak Agung Istri; Nurhayati, Nurhayati; Jayus, Jay
JURNAL AGROTEKNOLOGI Vol 17 No 02 (2023)
Publisher : Faculty of Agricultural Technology, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/j-agt.v17i02.41326

Abstract

The demand for xylose as a xylitol raw material is increasing. The resource for this material can be explored from agricultural waste such as lignocellulosic material which is widely available in nature, cheap, and renewable. The bioconversion of lignocellulose waste into xylose can use microbes, either single culture or microbial consortia. The purpose of this research is to find out the ability of Bacillus subtilis, Aureobasidium pullulans, and Penicillium janczewskii to convert xylan of cassava waste to produce xylose, under both single individual culture and its consortia. The xylose release from the cultures were measured using DNS tests followed by HPLC determination. The results showed that the single microbial culture of B. subtilis, A. pullulans, and P. janczewskii was able to produce xylose released into the culture broth which was 75.06, 81.72, and 82.00 ppm respectively, indicating the xylanolytic enzymes activities from this microorganism which have the ability to convert xylan into xylose. Unexpectedly, all the mix culture of these microbial consortia were unable to produce higher xylose, the amount of xylose released were even lower became 63.11 ppm only when B. subtilis, A. pullulans, and P. janczewskii worked together in a simultaneous culture. This finding indicated that these three microorganisms might not be able to hydrolysed the xylan synergistically. Keywords: bioconversion, lignocellulosic material, microbial consortia, xylanolytic enzyme
Development of Dihydrofolate Reductase Inhibitor Based on QSAR and Molecular Docking Sudarko, Sudarko; Kristiyono, Rimba Candra; Ratnadewi, Anak Agung Istri; Handayani, Wuryanti
Indonesian Chimica Letters Vol. 3 No. 1 (2024)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/icl.v3i1.940

Abstract

QSAR modeling allows for predicting activity through quantitative relationships between molecular structure and activity. This research uses DEEPScreen, which is a development of QSAR for searching new drugs. This research leverages DEEPScreen-QSAR modeling to optimize the predictive power of machine learning algorithms on a dataset of 645 molecules from previous research. The optimized model achieves an accuracy of 0.7461 and precision of 0.8169, demonstrating its effectiveness in the virtual screening stage. The optimized DEEPscreen-QSAR model is used to screen approximately 1.9 million small molecules in the ChEMBL database, resulting in binary classification predictions of active (1) molecules as 781,213 and inactive (0) molecules as 1,133,325 (molecules with IC50 activity ≤10,000 nM are considered active). The active (1) molecules obtained are screened again to find molecules that can be absorbed by the body (orally) using Lipinski’s RO5 with 0 deviations, resulting in 557,428 active molecules that can be absorbed by the body. These screening results are validated using molecular docking methods by linking protein and ligand to determine Gibbs free energy (∆G) and interactions using PyRx, PyMOL, and Biovia Discovery Studio programs. Based on the results of this research, candidate DHFR inhibitors with codes CHEMBL3302655, CHEMBL1384989, and CHEMBL1729486 are recommended.
Co-Authors Agung Budi Santoso Andika Ade Kurniawan Andika Ade Kurniawan, Andika Ade Andriana Kusuma Pertiwi Aprilia, Selvina Rizky Arianti, Fefpi Nur Afnifitri Wias Asnawati Bambang Piluharto Dwi Indarti Eka Yuni Kurniawati Eka Yuni Kurniawati, Eka Yuni Fauziyah, Kamelia Rizqi Febriani, Nurul Afifah Firda Marta Safitri Hefinda Erfiandika Indras Dwi Anggita Jayus, Jay Kristiyono, Rimba Candra Muhamad Kiki Afindia Joenata Nasrul Amaliyatun Naja Nasution, Annio Indah Lestari Nurhayati Nurhayati Nyoman Gede Krishnabudi Rosa Safitri Selvi Marcellia Siti Nur Avida Sudarko Sudarko Sudarko Wuryanti Handayani Yulvia, Ana