Article Per Year (5 Year)
p-Index From 2021 - 2026
1.376
P-Index
This Author published in this journals
All Journal Jurnal AGROTEKNOLOGI Jurnal Ilmu Dasar BERKALA SAINSTEK UNEJ e-Proceeding Jurnal Kimia Riset Industrial Research Workshop and National Seminar Indonesian Chimica Letters Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) Journal of Bio-molecule Research And Engineering
Anak Agung Istri Ratnadewi
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, University Of Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Education Electrical & Electronics Engineering Engineering Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Mechanical Engineering Physics Other

Published : 14 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : UNEJ e-Proceeding

 1 
Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production Ratnadewi, Anak Agung Istri; Kurniawan, Andika Ade; Handayani, Wuryanti
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer  have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among  the other XOS   X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm). Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase
Transformation of Plasmid pET Endo-1,4-β-xilanase from E. coli TOP10 to E. coli BL21 Santoso, Agung Budi; Kurniawati, Eka Yuni; Ratnadewi, Anak Agung Istri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In this research we successfully transmited Plasmid pET- Endo from E.coli TOP10 to E.coli BL21. Plasmid pET Endo is recombinant plasmid base on pET-30a(+)  inserted with gene of endo-1,4-β-xilanase isolated from bacillus subtilis sp which is originally living in termite abdomen. First step is isolation of plasmid pET-Endo from E.coli TOP10 with alkaline lyses. Denaturation and renaturation of DNA occurred then separated with centrifugation. Plasmid pET-Endo is smaller than Chromosome DNA. Agarose gel electrophoresis confirmed that Plasmid pET-Endo has isolated. Electrogram of pET-Endo show the band in 6022 bp compare to empty plasmid pET-30a(+) 5422 bp. E.Coli BL21 got pretreatment with  CaCl2 solution to make cell competent for transformation. Isolated pET-Endo inserted to E.coli BL21 with heat shock method. Resistance test with antibiotic has done to know the result of transformation. E.coli BL21 contained plasmid pET-Endo will survive in kanamycin agar media. Colony of E.coli BL21 then cultured in liquid media and examinee it’s growth phase. IPTG as gene inducer given after 2,5 hour of inoculation. Crude enzyme tested for xilanase activity and well proved. Isolation of plasmid pET-Endo then running in Agarose gel electrophoresis also confirm good result of transformation.
Transformation of Plasmid pET Endo-1,4-β-xilanase from E. coli TOP10 to E. coli BL21 Agung Budi Santoso; Eka Yuni Kurniawati; Anak Agung Istri Ratnadewi
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In this research we successfully transmited Plasmid pET- Endo from E.coli TOP10 to E.coli BL21. Plasmid pET Endo is recombinant plasmid base on pET-30a(+)  inserted with gene of endo-1,4-β-xilanase isolated from bacillus subtilis sp which is originally living in termite abdomen. First step is isolation of plasmid pET-Endo from E.coli TOP10 with alkaline lyses. Denaturation and renaturation of DNA occurred then separated with centrifugation. Plasmid pET-Endo is smaller than Chromosome DNA. Agarose gel electrophoresis confirmed that Plasmid pET-Endo has isolated. Electrogram of pET-Endo show the band in 6022 bp compare to empty plasmid pET-30a(+) 5422 bp. E.Coli BL21 got pretreatment with  CaCl2 solution to make cell competent for transformation. Isolated pET-Endo inserted to E.coli BL21 with heat shock method. Resistance test with antibiotic has done to know the result of transformation. E.coli BL21 contained plasmid pET-Endo will survive in kanamycin agar media. Colony of E.coli BL21 then cultured in liquid media and examinee it’s growth phase. IPTG as gene inducer given after 2,5 hour of inoculation. Crude enzyme tested for xilanase activity and well proved. Isolation of plasmid pET-Endo then running in Agarose gel electrophoresis also confirm good result of transformation.
Optimization pH of Enzymatic Hydrolysis of Endo-1,4-β-Xylanase for Xylooligosaccharides Production Anak Agung Istri Ratnadewi; Andika Ade Kurniawan; Wuryanti Handayani
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Xylooligosaccharides (XOS) with polymerisation degree between 2 till 10 monomer  have prebiotic effect for better digestion system. In this research, production of XOS was performed by enzymatic hydrolysis of xylan with endo-1,4-β-xylanases enzyme. Endo 1,4-β-xylanases enzyme was aqcuired from Bacillus sp. isolated from termite’s abdominal.Meanwhile oat xylan was used as substrate. Optimal condition of enzymatic hydrolysis was evaluated at pH: 4, 5, 6, and 7 with incubation time from 5 to 20 h.pH 5 was optimum pH to produce XOS from 0.8 % xylan oat at 40 oC.The hydrolysis product purified and analyzed by thin layer chromatographyyielding spot of xylobiose, xylotriose, xilotetraose and xilopentaose. Further analysis by HPLC indicated dominant xilopentaosa X5 (3522 ppm) among  the other XOS   X2 (14 ppm), X3 (43 ppm) and X4 (15 ppm). Keywords: Xylooligosaccharides, endo-1,4-β- endoxylanase
Co-Authors Agung Budi Santoso Andika Ade Kurniawan Andika Ade Kurniawan, Andika Ade Andriana Kusuma Pertiwi Aprilia, Selvina Rizky Arianti, Fefpi Nur Afnifitri Wias Asnawati Bambang Piluharto Dwi Indarti Eka Yuni Kurniawati Eka Yuni Kurniawati, Eka Yuni Fauziyah, Kamelia Rizqi Febriani, Nurul Afifah Firda Marta Safitri Hefinda Erfiandika Indras Dwi Anggita Jayus, Jay Kristiyono, Rimba Candra Muhamad Kiki Afindia Joenata Nasrul Amaliyatun Naja Nasution, Annio Indah Lestari Nurhayati Nurhayati Nyoman Gede Krishnabudi Rosa Safitri Selvi Marcellia Siti Nur Avida Sudarko Sudarko Sudarko Wuryanti Handayani Yulvia, Ana