Article Per Year (5 Year)
p-Index From 2020 - 2025
0.778
P-Index
This Author published in this journals
All Journal Agrointek BERITA BIOLOGI Jurnal Kedokteran Hewan Jurnal Tumbuhan Obat Indonesia
Harto Widodo, Harto
Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional Tawangmangu, Tawangmangu
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
KERAGAMAN GENETIK ALANG-ALANG (Imperata cylindrica (L.) Beauv.) BERDASARKAN MARKA INTER-SIMPLE SEQUENCE REPEATS (ISSR) Subositi, Dyah; Widodo, Harto
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4635.991 KB) | DOI: 10.14203/beritabiologi.v17i2.2642

Abstract

Alang-alang (Imperata cylindrica (L.) Beauv.) has been widely used as a medicinal plant to treat some diseases, such as fever, headache, and diuretic. Nowadays, there is no information of genetic diversity of this plant used in herbal formula by ethnic groups in Indonesia. The main objective of this study was to asses genetic diversity of alang-alang from 18 selected ethnic groups in Indonesia based on Inter Simple Sequence Repeats (ISSR). Location of sample collection was identified by using data on Research on Medicinal Plant (Ristoja) 2012. Total DNA genome was isolated and ISSR primer screening were done on collected samples. Ten selected ISSR primers produced 74 amplified DNA fragments 58, fragments (78.4 %) were polymorphic. Dice index similarity was used to construct UPGMA dendrogram. The genetic similarity indexing which among accessions was ranged from 70.5–90.5% thereby indicating a low level of genetic diversity occurred in alang-alang. The results of this study also showed that ISSR markers were able to genetically differentiate alang-alang accessions by which this information can be useful for further researchs such as for standardization of medicinal plants. 
Medicinal Plants used for Antihypercholesterolemia in Ethnic Groups of Celebes Island, Indonesia: Pemanfaatan Tumbuhan Obat sebagai Antihiperkolesterolemia di Berbagai Etnis di Pulau Sulawesi, Indonesia Rahmawati, Nuning; Haryanti, Sari; Yanti Marfuatush Sholikhah, Ika; Subositi, Dyah; Widodo, Harto; Mujahid, Rohmat; Widiyastuti, Yuli
Jurnal Tumbuhan Obat Indonesia Vol. 16 No. 2 (2023): December 2023
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v16i2.1025

Abstract

This study aimed to identify the utilization of medicinal plants used by selected traditional healers who met inclusion criteria to treat high cholesterol levels in ethnic groups of Celebes Island, Indonesia. Data collection was carried out through interviews, observation, and sample collection. This study revealed 67 concoction information and identified the use of 48 plant species distributed in 32 families among 39 healers in 20 ethnic groups in Celebes Island. Peperomia pellucida (L.) Kunth (UV=0.21), lamiaceae (15.63%), and leaves (57.35%) were determined as the most prominent species, family, and plant part used. The most widely prescribed rule of use is internal administration (94.03%) with a frequency of 3x1 per day (44.78%) for a week to a month (56.72%). However, conservation efforts need to be initiated immediately since more than 50% of healers made no cultivation efforts
Quality of Clarias sp with the addition of ginger (Zingiber officinale) and turmeric (Curcuma longa) in cold storage Fitriyanto, Akbar; Suhartatik, Nanik; Nur'aini, Vivi; Widodo, Harto
AGROINTEK Vol 19, No 3 (2025)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v19i3.24022

Abstract

Apart from being sold in whole form, fish is also sold in ready-to-use fillets. Fillets were easier for buyers because they were practical, easy to use, and clean. The disadvantage of serving in the form of fillets was that they were very susceptible to damage due to the ease of damage. Fish fillets have a high moisture and protein content. Without any protection, this material will quickly deteriorate. This study aimed to determine the effect of ginger and turmeric extracts on catfish fillets stored at cold temperatures The study used a factorial complete randomized design with the ratio of ginger and turmeric extracts as the first factor and storage days as the second factor. The results show that the use of ginger and turmeric as additional ingredients in the storage of catfish fillets was less effective in inhibiting fillet deterioration. The moisture content of the catfish fillets decreased from 82.66% to 70.51% after 6 days of cold storage. The pH value of the catfish fillet increased, while water-soluble protein and TVB-N were varied for some treatments. The pH value of fresh fish fillets without the addition of ginger and turmeric was 5.50 and reached 6.30 after 6 days of cold storage. Fillets with turmeric and ginger have a lower pH (6.15), and the ratio of the rhizome extracts did not significantly affect the pH value. According to the TVBN value, ginger and turmeric extracts could maintain the quality of fish fillets for up to 4 days at cold temperatures. Further research needs to be done to study the effect of packaging on the fresh fillet quality.
Aplikasi edible coating pati garut (Maranta arundinacea) pada apel (Malus sylvestris) potong dengan penambahan jeruk nipis dan daun mint Junianto, Faizal; Widodo, Harto; Suhartatik, Nanik; Karyantina, Merkuria
AGROINTEK Vol 19, No 4 (2025)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v19i4.27761

Abstract

An edible coating is a thin layer of hydrocolloid materials such as polysaccharides, polypeptides, lipids, and composites. Apples are one of the fruits that often undergo minimal processing, such as slicing. Cut apples are susceptible to damage, which can lead to rapid browning, to slow this reaction, an edible coating can be applied. This study aimed to determine the quality of apple slices after being treated with an edible coating made from arrowroot starch with lime juice and mint leaf during storage. This study used a Completely Randomised Design with two factors: storage time (0, 2, 4, and 6 days) and the concentration of lime juice and mint leaf. The results showed that adding lime juice and mint leaf did not significantly affect water content, vitamin C, and weight loss of apple slices, while the longer the storage, the more damaged the sliced apples were. Weight loss, antioxidant activity, total phenols, vitamin C, and total aerobic bacteria decreased during storage. The treatment of adding 13% lime juice and 10% mint leaves was able to suppress the total number of bacteria to 5.77 log 10 CFU / g at a shelf life of 6 days. The treatment of adding mint leaves and lime extract was able to inhibit damage to sliced apples for 6 days of storage
KANDIDAT PROBE DNA DARI URUTAN REPETITIF R529 UNTUK DETEKSI Toxoplasma gondii Hartati, Sri; Kusumawati, Asmarani; Widodo, Harto; Priyo Widodo, Dwi
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2631

Abstract

Penelitian ini bertujuan merancang kandidat probe dari urutan 529 bp (R529) yang terdapat 200-300 kopi dalam genom Toxoplasma gondii (T. gondii). Untuk menguji spesifitas rancangan probe digunakan perangkat lunak basic local alignment search tool (BLASTN) dari national center for biotechnology information (NCBI) dan Geniuous. Probe DNA sebesar 237 bp dilabel yang dengan digoksigenin disintesis dengan polymerase chain reaction (PCR). Kandidat probe yang dihasilkan berpotensi untuk digunakan lebih lanjut dalam deteksi keberadaan asam nukleat T. gondii pada berbagai teknik hibridisasi.
Medicinal Plants used for Antihypercholesterolemia in Ethnic Groups of Celebes Island, Indonesia: Pemanfaatan Tumbuhan Obat sebagai Antihiperkolesterolemia di Berbagai Etnis di Pulau Sulawesi, Indonesia Rahmawati, Nuning; Haryanti, Sari; Yanti Marfuatush Sholikhah, Ika; Subositi, Dyah; Widodo, Harto; Mujahid, Rohmat; Widiyastuti, Yuli
Jurnal Tumbuhan Obat Indonesia Vol. 16 No. 2 (2023): December 2023
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v16i2.1025

Abstract

This study aimed to identify the utilization of medicinal plants used by selected traditional healers who met inclusion criteria to treat high cholesterol levels in ethnic groups of Celebes Island, Indonesia. Data collection was carried out through interviews, observation, and sample collection. This study revealed 67 concoction information and identified the use of 48 plant species distributed in 32 families among 39 healers in 20 ethnic groups in Celebes Island. Peperomia pellucida (L.) Kunth (UV=0.21), lamiaceae (15.63%), and leaves (57.35%) were determined as the most prominent species, family, and plant part used. The most widely prescribed rule of use is internal administration (94.03%) with a frequency of 3x1 per day (44.78%) for a week to a month (56.72%). However, conservation efforts need to be initiated immediately since more than 50% of healers made no cultivation efforts
Co-Authors Asmarani Kusumawati Dwi Priyo Widodo, Dwi Dyah Subositi Fitriyanto, Akbar Junianto, Faizal Merkuria Karyantina Nanik Suhartatik Nuning Rahmawati Nur'aini, Vivi Rohmat Mujahid, Rohmat Sari Haryanti Sri Hartati Yanti Marfuatush Sholikhah, Ika Yuli Widiyastuti