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All Journal Agrointek BERITA BIOLOGI Jurnal Kedokteran Hewan Jurnal Tumbuhan Obat Indonesia
Harto Widodo, Harto
Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional Tawangmangu, Tawangmangu
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : BERITA BIOLOGI

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KERAGAMAN GENETIK ALANG-ALANG (Imperata cylindrica (L.) Beauv.) BERDASARKAN MARKA INTER-SIMPLE SEQUENCE REPEATS (ISSR) Subositi, Dyah; Widodo, Harto
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4635.991 KB) | DOI: 10.14203/beritabiologi.v17i2.2642

Abstract

Alang-alang (Imperata cylindrica (L.) Beauv.) has been widely used as a medicinal plant to treat some diseases, such as fever, headache, and diuretic. Nowadays, there is no information of genetic diversity of this plant used in herbal formula by ethnic groups in Indonesia. The main objective of this study was to asses genetic diversity of alang-alang from 18 selected ethnic groups in Indonesia based on Inter Simple Sequence Repeats (ISSR). Location of sample collection was identified by using data on Research on Medicinal Plant (Ristoja) 2012. Total DNA genome was isolated and ISSR primer screening were done on collected samples. Ten selected ISSR primers produced 74 amplified DNA fragments 58, fragments (78.4 %) were polymorphic. Dice index similarity was used to construct UPGMA dendrogram. The genetic similarity indexing which among accessions was ranged from 70.5–90.5% thereby indicating a low level of genetic diversity occurred in alang-alang. The results of this study also showed that ISSR markers were able to genetically differentiate alang-alang accessions by which this information can be useful for further researchs such as for standardization of medicinal plants. 
Co-Authors Asmarani Kusumawati Dwi Priyo Widodo, Dwi Dyah Subositi Fitriyanto, Akbar Junianto, Faizal Merkuria Karyantina Nanik Suhartatik Nuning Rahmawati Nur'aini, Vivi Rohmat Mujahid, Rohmat Sari Haryanti Sri Hartati Yanti Marfuatush Sholikhah, Ika Yuli Widiyastuti