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IDENTIFIKASI APOPTOSIS DENGAN METODE TUNEL PASCA PEMBERIAN EKSTRAK SAMBILOTO DAN PENGARUHNYA TERHADAP VOLUME TUMOR WH, Nugrahaningsih; Yuniastuti, Ari
Sainteknol : Jurnal Sains dan Teknologi Vol 13, No 1 (2015): June 2015
Publisher : Unnes Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v13i1.5335

Penelitian ini dilakukan untuk membuktikan pengaruh ekstrak sambiloto terhadap apoptosis dan volume tumor bila diberikan secara oral dan diidentifikasi dengan metode TUNEL. Penelitian dilakukan dengan desain Randomized post test control group. Sebanyak 24 ekor mencit C3H yang telah tumbuh kanker mamma dibagi secara acak menjadi 4 kelompok masing-masing 6 ekor. Diberikan ekstrak sambiloto dengan dosis 5, 10 dan 15 mg per ekor per hari, dan kelompok kontrol. Pemberian ekstrak sambiloto dilakukan secara oral selama 14 hari. Pengukuran volume tumor dilakukan pada hari ke 1, 4, 8, 12 dan 15. Pada hari ke 15 mencit dimatikan dengan menggunakan eter. Jaringan kanker dibuat blok parafin untuk pemeriksaan apoptosis dengan metode TUNEL. Hasil Penelitian menunjukkan adanya peningkatan signifikan sel apoptosis (p=0,000), dengan dosis terbaik 15 mg/hari. Ada beda yang bermakna pada rerata kelipatan penambahan volume tumor (p=0,049). Hasil uji post hoc menunjukkan bahwa perbedaan terletak antara kelompok kontrol dengan kelompok dosis 10 mg/hr. Dapat disimpulkan bahwa pemberian ekstrak sambiloto secara oral meningkatkan sel yang mengalami apoptosis dengan pemeriksaan TUNEL namun belum dapat menurunkan volume tumor.

Pengaruh Pemberian Vitamin A terhadap Penurunan Parasitemia Mencit Strain Swiss yang diinfeksi Plasmodium berghei Isnaeni, Umi; Iswari, Retno Sri; WH, Nugrahaningsih; Susanti, R.
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.3930

Vitamin A merupakan vitamin yang berperan sebagai imunostimulan. Penelitian ini bertujuan untuk mengetahui apakah pemberian vitamin A dapat menurunkan parasitemia Plasmodium berghei pada mencit strain Swiss. Penelitian ini menggunakan 24 ekor mencit strain Swiss jantan berumur 6-8 minggu dengan berat badan 20-30 gram/ekor. Penelitian dilaksanakan secara eksperimental dengan menggunakan time series design. Dalam penelitian ini dilakukan perlakuan berupa pemberian vitamin A dengan 3 variasi dosis yaitu 0 IU/g BB, 35 IU/g BB dan 70 IU/g BB serta kelompok kontrol negatif dengan masing- masing kelompok terdiri dari 6 ekor mencit. Vitamin A diberikan 1 jam sebelum penginfeksian dan mencit dirawat sampai mencit pada kelompok kontrol negatif mati. Sediaan apus darah tipis dibuat 2 hari sekali dan parasitemia dihitung dengan pengecatan Giemsa. Data parasitemia dianalisis dengan ANOVA.Untuk hasil yang signifikan maka dilanjutkan dengan uji lanjut Post hoc pada taraf kesalahan 1%. Hasil uji ANOVA untuk kelompok perlakuan B, C dan D diperoleh nilai p <0,001 pada masing-masing kelompok perlakuan. Hal tersebut menyatakan bahwa adanya perbedaan yang signifikan pada perlakuan yang diberikan. Begitu juga untuk uji lanjut Post hoc yang telah dilakukan diperoleh nilai p < 0,001. Dapat disimpulkan bahwa pemberian vitamin A berpengaruh terhadap penurunan parasitemia Plasmodium berghei pada mencit strain Swiss.Vitamin A is a vitamin that acts as an immunostimulant. This research aims to determine whether the administration of vitamin A can reduce parasitaemia of Plasmodium berghei in Switzerland strain mice. This research used 24 mice Switzerland strain mice 6-8 weeks old weighing 20-30 grams/tail. Research conducted experimentally by using time series design. In this research, the provision of vitamin A treatment with 3 doses of variation is 0 IU/g BW, 35 IU/g BW and 70 IU/g BW as well as the negative control group, with each group consisting of 6 mice. Vitamin A was given 1 hour before infection then treated mice to mice in the negative control group died. Thin blood smears were made 2 days and parasitaemia was calculated by Giemsa staining. Parasitaemia data were analyzed with ANOVA. Significant results then followed by a further test Post hoc at 1% error level. ANOVA test results for the treatment group B, C and D obtained value of p <0.001 for each treatment group. It is claimed that there are significant differences in the treatment given. Likewise for further Post hoc testing that has been done obtained value of p <0.001. It can be concluded that the administration of vitamin A affects the reduction in parasitaemia of Plasmodium berghei in Switzerland strain mice.

The Development of Nervous Problem Based Modulewith Character Conservation for Nervous System Materials in the Senior High School Qudsiya, Reefa; Lisdiana, Lisdiana; WH, Nugrahaningsih
Journal of Biology Education Vol 7 No 3 (2018): December 2018
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v7i3.26853

Nervous system teaching materials available in schools do not contain scientific-related content as mandated by the 2013 curriculum. In addition, teaching materials also do not contain student character reinforcement content. The purpose of this study is to develop “Nervous Problem Based Module with a  Conservation Character” as a nervous system learning supplement. This type of research is Research and Development (R&D) using ten steps, namely identification of potential and problems, data collection, product design, product design validation, product design revisions, small scale trials, product revision I, large scale trials, product revision II, and final products. The research subjects were students of class XI of SMA N 1 Bae Kudus. Nervous Problem Based Module with a  Conservation Character  tested the feasibility, readability, and effectiveness. Feasibility tests obtained very valid results. Readability test results were very valid. Test effectiveness by applying  Nervous Problem Based Module with a  Conservation Character in learning, obtained effective results and can be used in learning. The insertion of the conservation character reinforcement in the module gives positive results on the character of students. It can be concluded that “Nervous Problem Based Module with a Conservation Character” is decent and effectively applied in the nervous system learning.

IDENTIFIKASI APOPTOSIS DENGAN METODE TUNEL PASCA PEMBERIAN EKSTRAK SAMBILOTO DAN PENGARUHNYA TERHADAP VOLUME TUMOR WH, Nugrahaningsih; Yuniastuti, Ari
Sainteknol : Jurnal Sains dan Teknologi Vol 13, No 1 (2015): June 2015
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v13i1.5335

Penelitian ini dilakukan untuk membuktikan pengaruh ekstrak sambiloto terhadap apoptosis dan volume tumor bila diberikan secara oral dan diidentifikasi dengan metode TUNEL. Penelitian dilakukan dengan desain Randomized post test control group. Sebanyak 24 ekor mencit C3H yang telah tumbuh kanker mamma dibagi secara acak menjadi 4 kelompok masing-masing 6 ekor. Diberikan ekstrak sambiloto dengan dosis 5, 10 dan 15 mg per ekor per hari, dan kelompok kontrol. Pemberian ekstrak sambiloto dilakukan secara oral selama 14 hari. Pengukuran volume tumor dilakukan pada hari ke 1, 4, 8, 12 dan 15. Pada hari ke 15 mencit dimatikan dengan menggunakan eter. Jaringan kanker dibuat blok parafin untuk pemeriksaan apoptosis dengan metode TUNEL. Hasil Penelitian menunjukkan adanya peningkatan signifikan sel apoptosis (p=0,000), dengan dosis terbaik 15 mg/hari. Ada beda yang bermakna pada rerata kelipatan penambahan volume tumor (p=0,049). Hasil uji post hoc menunjukkan bahwa perbedaan terletak antara kelompok kontrol dengan kelompok dosis 10 mg/hr. Dapat disimpulkan bahwa pemberian ekstrak sambiloto secara oral meningkatkan sel yang mengalami apoptosis dengan pemeriksaan TUNEL namun belum dapat menurunkan volume tumor.

DEVELOPMENT OF INTERACTIVE MULTIMEDIA FOR HUMAN REPRODUCTION SYSTEM IN JUNIOR HIGH SCHOOL Puspitasari, Dina Nafisa; Indriyanti, Dyah Rini; WH, Nugrahaningsih
Journal of Biology Education Vol 8 No 2 (2019): August 2019
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v8i2.28016

The study was aim to find out the effectiveness of the multimedia interactive for human reproduction system learning.  The research was done at Junior High School 5 Pemalang with the design of research is Research and Development (R&D). The small scale trial of the product was done in 23 students with proportionate stratified random sampling method. The larger scale trial of the product was done in IX A class with Purposive Sampling Method and One Group Pre-test Post-test Experimental Design. The assessment from the experts to the multimedia interactive for system human reproduction learning obtains a high score as much as 89.58% for course assessment and 97.22% for media assessment. The results from the teacher and students response questionnaire in small scale trial obtain the average percentage as much as 100 % and 81.55 % respectively, which was high qualified. The effectiveness of Multimedia Interactive in learning could be seen in pretest and post test results with calculating N-gain and obtain N-gain 0.54, which was categorized as middle. Beside N-gain, the completeness of understanding the concepts become another effectiveness of multimedia interactive obtain 88% in classical completeness.   The results of the study shows that the multimedia interactive was qualified and effective to enhance the students? learning outcome in the course of system human reproduction.

APPLICATION OF THE PROBLEM BASED-FISHBONE MODEL ON REPRODUCTIVE SYSTEM MATERIAL IN SMA N 1 DEMPET WH, Nugrahaningsih; ., Lisdiana; Subekti, Arum
Journal of Biology Education Vol 8 No 3 (2019): December 2019
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v8i3.29430

Students achievement that are less than optimal indicate the students thinking skills are not maximal. To improve students thinking skills can be done in several ways among others, students further deepen  the concept of material, to provide further explanation and to provide alternative solutions to problems, so that students in the future are expected to solve problems in the environment. One learning model that can improve students thinking skills is problem based learning. Problem-based  learning models can encourage students ability to conduct investigations and be able to provide alternative solutions to problems. This study aims to analyze the model problem based -fishbone on reproductive system material influencing learning outcomes and the health care attitude of high school students. This study used a quasi experimental design with  pretest-posttest control group design. The sample of this study was students of class XI MIPA 2 and XI MIPA 6 in SMA N 1 Dempet which were determined using purposive sampling technique. The independent variable is a model problem based-fishbone on reproductive system material. Dependent variables are student learning outcomes and student health care attitudes. How to retrieve data using test and observation sheet questions. The results of data analysis showed that student learning outcomes could increase after learning activities were completed with completeness for both classes in a row 79.54% and 90.48%, and students had good health care attitudes. Based on the results of the study it can be concluded that the application of the model problem based- fishbone influences learning outcomes and health care attitudes in high school students.

The Development of Nervous Problem Based Modulewith Character Conservation for Nervous System Materials in the Senior High School Qudsiya, Reefa; Lisdiana, Lisdiana; WH, Nugrahaningsih
Journal of Biology Education Vol 7 No 3 (2018): December 2018
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v7i3.26853

Nervous system teaching materials available in schools do not contain scientific-related content as mandated by the 2013 curriculum. In addition, teaching materials also do not contain student character reinforcement content. The purpose of this study is to develop â€œNervous Problem Based Module with a Conservation Characterâ€ as a nervous system learning supplement. This type of research is Research and Development (R&D) using ten steps, namely identification of potential and problems, data collection, product design, product design validation, product design revisions, small scale trials, product revision I, large scale trials, product revision II, and final products. The research subjects were students of class XI of SMA N 1 Bae Kudus. Nervous Problem Based Module with a Conservation Character tested the feasibility, readability, and effectiveness. Feasibility tests obtained very valid results. Readability test results were very valid. Test effectiveness by applying Nervous Problem Based Module with a Conservation Character in learning, obtained effective results and can be used in learning. The insertion of the conservation character reinforcement in the module gives positive results on the character of students. It can be concluded that â€œNervous Problem Based Module with a Conservation Characterâ€ is decent and effectively applied in the nervous system learning.

Development of Interactive Multimedia for Human Reproduction System in Junior High School Puspitasari, Dina Nafisa; Indriyanti, Dyah Rini; WH, Nugrahaningsih
Journal of Biology Education Vol 8 No 2 (2019): August 2019
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v8i2.28016

The study was aim to find out the effectiveness of the multimedia interactive for human reproduction system learning. The research was done at Junior High School 5 Pemalang with the design of research is Research and Development (R&D). The small scale trial of the product was done in 23 students with proportionate stratified random sampling method. The larger scale trial of the product was done in IX A class with Purposive Sampling Method and One Group Pre-test Post-test Experimental Design. The assessment from the experts to the multimedia interactive for system human reproduction learning obtains a high score as much as 89.58% for course assessment and 97.22% for media assessment. The results from the teacher and students response questionnaire in small scale trial obtain the average percentage as much as 100 % and 81.55 % respectively, which was high qualified. The effectiveness of Multimedia Interactive in learning could be seen in pretest and post test results with calculating N-gain and obtain N-gain 0.54, which was categorized as middle. Beside N-gain, the completeness of understanding the concepts become another effectiveness of multimedia interactive obtain 88% in classical completeness. The results of the study shows that the multimedia interactive was qualified and effective to enhance the studentsâ€™ learning outcome in the course of system human reproduction.

Application of the Problem Based-Fishbone Model on Reproductive System Material in SMA N 1 Dempet Subekti, Arum; WH, Nugrahaningsih; ., Lisdiana
Journal of Biology Education Vol 8 No 3 (2019): December 2019
Publisher : FMIPA UNNES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jbe.v8i3.29430

Students achievement that are less than optimal indicate the students thinking skills are not maximal. To improve students thinking skills can be done in several ways among others, students further deepen the concept of material, to provide further explanation and to provide alternative solutions to problems, so that students in the future are expected to solve problems in the environment. One learning model that can improve students thinking skills is problem based learning. Problem-based learning models can encourage students ability to conduct investigations and be able to provide alternative solutions to problems. This study aims to analyze the model problem based -fishbone on reproductive system material influencing learning outcomes and the health care attitude of high school students. This study used a quasi experimental design with pretest-posttest control group design. The sample of this study was students of class XI MIPA 2 and XI MIPA 6 in SMA N 1 Dempet which were determined using purposive sampling technique. The independent variable is a model problem based-fishbone on reproductive system material. Dependent variables are student learning outcomes and student health care attitudes. How to retrieve data using test and observation sheet questions. The results of data analysis showed that student learning outcomes could increase after learning activities were completed with completeness for both classes in a row 79.54% and 90.48%, and students had good health care attitudes. Based on the results of the study it can be concluded that the application of the model problem based- fishbone influences learning outcomes and health care attitudes in high school students.

THE EFFECT OF RAMBUTAN PEEL EXTRACT (NEPHELIUM LAPPACEUM L) TO TOTAL LEUKOCYTES AND HISTOPATHOLOGICAL OF RAT LUNGS EXPOSED BY CIGARETTE SMOKE Lisdiana, Lisdiana; WH, Nugrahaningsih; Widyaningrum, Priyantini
Sainteknol : Jurnal Sains dan Teknologi Vol 15, No 2 (2017): December 2017
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/sainteknol.v15i2.12404

Cigarette smoke is one of exogenous free radicals sources. When it is inhaled, its activity may damage the structure of lung function. The impacts of free radicals can be reduced through the provision of antioxidants. Rambutan fruit peel contains phenolic compound in the form of polyphenols that are antioxidants.The purpose of this study is to determine the effect of rambutan fruit peel extracts to the number of leukosit and lung structure on rats exposed by cigarette smoke. The research design used Post Test Control Group Design. A sample of 25 rats were divided into 5 groups, each group consisting of 5 rats. The positive control group (K +) were given a standard food and drinking water, the negative control group (K) by 3 cigarettes, the treatment groups (KP1, KP2, KP3) by 3 cigarettes and peel extract of rambutan each treatment group with a dose 15 mg/kg, 30 mg/kg and 45 mg/kg for 30 days. Total leukocyt measured by Auto Hematology Analyzer Mindray BC 2800vet. Total leukocytes and the damage of the lung were tested by one- way ANOVA. The number of leukocytes and damage to the lungs was analyzed by one-way ANOVA. and further tested with LSD. Next, the effective dose was tested by using linear regression. The result has shown that the average of total leukocytes in K+, K-, KP1, KP2, and KP3 was 6.28 x103/µl, 11.98 x103/µl, 9.88 x103/µl, 8.48 x103/µl, and 7.72 x103/µl respectively. Based on ANOVA, the rats have been exposed to cigarette smoke and given the extract of rambutan peel underwent decreasing total leukocytes and decreasing damage on alveolus more significant than those which has been exposed to cigarette smoke with no extract of rambutan peel. The effective dose of the extract of rambutan peel that was foun d to decrease total leukocytes and to protect the damage of the lung caused by cigarette smoke was 45 mg/kg body weight. Conclusion: the extract of rambutan peel was able to decrease the total leukocytes and protect the histopathological of rat lung that has been exposed to cigarette smoke.

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