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All Journal Jurnal Agrotek Tropika Jurnal Berkawan
Ummi Solikhah, Ummi
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Agriculture, Biological Sciences & Forestry Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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THE EFFECT OF TDZ (Thidiazuron) ON THE FORMATION OF DIRECT SOMATIC EMBRYOGENESIS IN Phalaenopsis sp. ORCHID Adinda, Intan Dwi; Restanto, Didik Pudji; Dewanti, Parawita; Munandar, Denna Eriani; Hartatik, Sri; Prayoga, Mohammad Candra; Solikhah, Ummi
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.9795

Abstract

Orchids (Phalaenopsis sp.) are among the most valuable ornamental plants due to their wide range of flower colors and high commercial demand. However, conventional propagation methods are often inefficient for large-scale production, necessitating the optimization of tissue culture techniques for rapid and uniform clonal propagation. The use of appropriate plant growth regulators, particularly thidiazuron (TDZ), is crucial for enhancing somatic embryogenesis—a key pathway for orchid micropropagation. This study aimed to determine the optimal concentration of TDZ for inducing somatic embryogenesis in Phalaenopsis sp. through histological analysis and scanning electron microscopy (SEM) observations. A completely randomized design was employed using Murashige and Skoog (MS) medium supplemented with four TDZ concentrations (1, 3, 5, and 7 mg/L). Leaf explants of Phalaenopsis sp. served as the explant source. The results demonstrated that direct somatic embryogenesis successfully occurred from leaf explants across treatments. Among the tested concentrations, 5 mg/L TDZ produced the most effective response, resulting in the highest somatic embryo formation rate (32%) and the shortest time to embryo maturation (37 days after culture initiation). The embryos exhibited characteristic dark green coloration and a crumbly texture. These findings highlight the pivotal role of TDZ in promoting somatic embryogenesis in Phalaenopsis sp., providing a reliable protocol for efficient orchid propagation. The study contributes to the advancement of orchid biotechnology by offering histological and ultrastructural evidence that supports the optimization of clonal propagation systems for commercial and conservation purposes.
PEMBERANTASAN SARANG NYAMUK DAN PENYULUHAN PHBS UNTUK MENCEGAH PENYEBARAN PENYAKIT DBD PADA MASYARAKAT DUKUH TEGALMANGU DESA KADOKAN Wardhana, Muhamad Fikry Abrar Yoga; Ahmad, Firhan; Syafatillah, Shureenzen Suci; Nadilla, Nur Dania Munfaatin; Salsabilla, Isnaini Tiara; Utami, Azida Alfian; Hafsyari, Hafidza Aulia; Anugraheni, Essa Andaru; Salsabilla, Gita Al-Fauzia; Adi, Inasya Safira Ramadiani Putri; Solikhah, Ummi; Puspitasari, Dyah Intan; Faozi, Ekan
Jurnal Berkawan: Jurnal Pengabdian kepada Masyarakat Vol. 3, No.1, Januari 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/berkawan.v3i1.6193

Abstract

Dengue Hemorrhagic Fever (DHF) is an acute febrile illness caused by infection with the Dengue Virus (DENV) and transmitted by Aedes aegypti mosquitoes. This disease is one of the most prevalent tropical diseases affecting humans. In Kadokan Village, DHF ranks as the leading public health concern due to the high number of cases, particularly in Tegalmangu Hamlet. This condition is closely associated with the community’s low awareness of the importance of Clean and Healthy Living Behavior (PHBS) in preventing the spread of DHF. This study aims to enhance community knowledge and awareness regarding the prevention and management of DHF through Mosquito Breeding Site Eradication (PSN) and the implementation of PHBS. The methods employed include observation, health education sessions, and the administration of pre-test and post-test questionnaires to assess the level of knowledge. The observational findings indicate behavioral changes related to environmental cleanliness, while the pre-and post-test results show a significance value of 0.012 (p<0.05), indicating a statistically significant difference in community knowledge before and after the educational intervention on DHF and PHBS.
Co-Authors Adi, Inasya Safira Ramadiani Putri Adinda, Intan Dwi Ahmad, Firhan Anugraheni, Essa Andaru Ekan Faozi Hafsyari, Hafidza Aulia Munandar, Denna Eriani Nadilla, Nur Dania Munfaatin Parawita Dewanti Prayoga, Mohammad Candra Puspitasari, Dyah Intan Restanto, Didik Pudji Salsabilla, Gita Al-Fauzia Salsabilla, Isnaini Tiara Sri Hartatik Syafatillah, Shureenzen Suci Utami, Azida Alfian Wardhana, Muhamad Fikry Abrar Yoga