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All Journal Jurnal Sains dan Teknologi JST ( Jurnal Sains Terapan ) JTT (Jurnal Teknologi Terpadu) Menara Perkebunan Specta Journal of Technology Cendekia : Jurnal Pengabdian Masyarakat Makara Journal of Science
Ashadi Sasongko
Institut Teknologi Kalimantan
Agriculture, Biological Sciences & Forestry Humanities Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Social Sciences Other

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Enzymatic Hydrolysis of Mannan from Konjac (Amorphophallus sp.) Using Mannanase from Streptomyces lipmanii to Produce Manno-oligosaccharides Sasongko, Ashadi; Yopi,; Rahmani, Nanik; Lisdiyanti, Puspita; Saepudin, Endang
Makara Journal of Science Vol. 19, No. 3
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mannan is an abundant polysaccharide that can be found in konjac (Amorphophallus sp.). Mannan can be enzymatically hydrolyzed using mannanase to produce manno-oligosaccharides which can be used as a prebiotic. The aims of this research are to determine the production time of mannanase from Streptomyces lipmanii, perform enzyme characterization, optimize the hydrolysis time, and characterize the hydrolysis product. A qualitative assay using the indicator Congo red showed that S. lipmanii generated a clear zone, indicating that S. lipmanii produced mannanase in konjac medium and possessed mannanolytic activity. Enzyme activity was determined through reducing sugar measurement using the dinitrosalycylic acid method, and optimum enzyme production was achieved at the second day of culture. Characterization of the enzyme showed that hydrolysis was optimum at pH 7 and at a temperature of 50 oC. The reducing sugar content was increased by an increasing the hydrolysis time, and reached an optimum time at 2 h. The degree of polymerization value of three was achieved after 2 h hydrolysis of mannan from konjac, indicating the formation of oligosaccharides. Analysis by thin layer chromatography using butanol, acetic acid, and water in a ratio of 2:1:1 as eluent showed the presence of compounds with a retention time between those of mannose and mannotetrose. Confirmation was also performed by HPLC, based on the retention time.
Studi Pengaruh Temperatur Kalsinasi dalam Pembentukan Nanomagnetite dengan Metode Green Synthesis Ekstrak Daun Nanas Putra Parmita, Ade Wahyu Yusariarta; Panji; Dewanto, Hizkia Alpha; Sasongko, Ashadi; Qulub, Fitriyatul; Triana, Yunita; Parmita, Ade Wahyu Yusariarta Putra
SPECTA Journal of Technology Vol. 7 No. 2 (2023): SPECTA Journal of Technology
Publisher : LPPM ITK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35718/specta.v7i2.940

Abstract

In synthesizing Fe3O4 nanoparticles using the green synthesis method by adding FeCl3.6H2O and pineapple leaf extract. Pineapple leaf extract is used as a natural reducing agent which contains hydroxyl and carbonyl, which acts as a reducing agent for the synthesized FeCl3.6H2O. Then after the precipitate is obtained, calcination will be carried out, where the calcination temperature in the synthesis becomes a factor in forming Fe3O4 nanoparticles. Different calcination temperatures of 400°C, 450°C, and 500°C were given to prepare Fe3O4 nanoparticles, with the XRD test results confirming that Fe3O4 nanoparticles were formed. In the SEM-EDX test, the optimum variation is 400°C because the average particle size is 150.23 nm with irregular particle shapes. Then proceed with the SAA results with a surface area of 60.125 m²/g. Nanomagnetite produced by green synthesis of pineapple leaf extract can be used as a removal dyes.
Aktivitas amilase bakteri amilolitik asal larva black soldier fly (Hermetia illucens) Irma KRESNAWATY; Rizki WAHYU; Ashadi SASONGKO
Menara Perkebunan Vol. 87 No. 2 (2019): 87 (2), 2019
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v87i2.341

Abstract

AbstractAmylase is an enzyme that has been widely used as a biocatalyst in foodand bioethanol industries. The availability of thermostable amylase will further expand the market and extend the shelf life of this enzyme. Amylase is produced by amylolytic bacteria using media with high-costnitrogen sources, such as pepton. Black soldier fly (BSF) is a potential source of amylolitic bacteria since its ability to degrade organic matters rapidly. This research aimedtoexploreamylolitic bacteria from the larvae of BSF with highest amylase activity that can be produced using low-cost media. The screening ofamylase activity was conducted by culturing the bacteria on starch containing media.Bacteria with the highest amylase activity were cultured in liquid media with twodifferentnitrogensources (urea and nitrate). Determinations of the optimum pH and temperature for this enzyme activity were carried out in the pH range 4to 7 and temperature 35to 65 ºC. Three amylase-producing isolates were obtained in this study. M1 isolate which has the highest activity was characterized based oncatalase activity and Gram staining. The results showed that the M1 isolate mightbelong togenus Proteussp. At the optimum condition (45ºC and pH 7), amylase activityin nitrate mediawas0.791U/mL, which was about 18-folds higher than that in ureamedia (0,041U/mL). Thus, amylase isolated from BSF larvae can be classified as a mesophilic enzyme and has the potential to be developed commercially at lower production costs.[Keywords:crude extract enzyme,Proteus sp.,thermostable] AbstrakAmilase merupakan salah satu enzim yangtelah digunakan secara luas sebagai biokatalis dalam industri pangan dan bioetanol.Ketersediaan amilase termostabil akan semakin memperluas pasar dan memperpanjang daya simpan enzim ini. Selama ini, produksi amilase dilakukan dengan memanfaatkan bakteri amilolitik menggunakan media dengan sumber nitrogen yang mahal, misalnya pepton. Black soldier fly (BSF) merupakan sumber bakteri amilolitik yang potential karena BSF memiliki kemampuan mendegradasi bahan organik dengan cepat.  Penelitian ini bertujuan untuk mengeksplorasi bakteri amilolitik dengan kemampuan amilase tinggi yang dapat diproduksi menggunakan media yanglebihmurah.Skrining bakteri penghasil amilase dilakukan dengan menumbuhkan bakteri pada media yang mengandung pati. Bakteri dengan aktivitas amilase tertinggi dikulturkan dalam media cair dengan dua sumber nitrogenyang berbeda, yaitu urea dan nitrat. PenentuanpH dan suhu optimum aktivitas enzim ini dilakukan pada rentang pH 4sampai 7 dan suhu 35sampai 65 ºC.Tiga isolat penghasil amilase diperoleh dalam penelitian ini. Isolat M1 yang memiliki aktivitas tertinggi dikarakterisasi berdasarkan uji katalasedan uji pewarnaan Gram. Hasilnya menunjukkan bahwa isolat M1 termasukgenus Proteus sp. Pada kondisi optimum (suhu 45oC dan pH 7), aktivitas amilase pada media nitrat adalah 0,791 U/mL, lebih kurang 18 kali lebih tinggi dibanding aktivitas pada media urea (0,041 U/mL). Dengan demikian, amilase yang dihasilkan oleh bakteri asallarva BSF merupakan enzim mesofilik dan berpotensi untuk dikembangkan secara komersial dengan biaya produksi yang lebih murah.[Kata kunci: enzim ekstrak kasar, Proteussp.,termostabil]
Verifikasi Metode Penentuan Logam Kadmium (Cd) dalam Air Limbah Domestik dengan Metode Spektrofotometri Serapan Atom Sasongko, Ashadi; Yulianto, Kurniawan; Sarastri, Dwi
JST (Jurnal Sains dan Teknologi) Vol. 6 No. 2 (2017)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (554.289 KB) | DOI: 10.23887/jstundiksha.v6i2.10699

Abstract

Kadmium (Cd) merupakan logam berat yang memiliki toksisitas yang tinggi. Kadar Cd perlu diketahui secara pasti karena di lingkungan perairan dapat terakumulasi dan dapat meracuni semua komponen biotik. Penentuan konsentrasi Cd dalam air limbah domestik dapat ditentukan menggunakan spektrofotometer serapan atom (SSA) nyala. Verifikasi metode SSA nyala diperlukan untuk membuktikan bahwa metode yang digunakan dapat dipercaya dan dapat dipertanggungjawabkan secara ilmiah. Parameter verifikasi metode analisis yang diuji yaitu linearitas, akurasi, presisi (keterulangan), limit deteksi, dan limit kuantitasi. Hasil verifikasi metode penentuan Cd dalam air limbah domestik dengan SSA telah memenuhi syarat. Persamaan regresi dari kurva standar yang diperoleh yaitu y= 0.0011+0.0414x dengan r sebesar 0.9998, % recovery berkisar 115.20% - 119.20% dengan rerata 116.91% dan % RSD yang sebesar 0.69%, lebih kecil dibandingkan dengan 2/3 CV Horwitz yaitu 12.88%. Limit deteksi dan limit kuantitasi berturut-turut sebesar 0.0045 ppm dan 0.0151 ppm. Metode penentuan cadmium dalam air limbah domestic dapat diverifikasi dan digunakan untuk analisis sample rutin.
Ammonia Determination In Bottled Water Using Spectrophotometer : Comparison Between Nessler And Berthelot Methods Sasongko, Ashadi
JST (Jurnal Sains dan Teknologi) Vol. 7 No. 1 (2018)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.42 KB) | DOI: 10.23887/jstundiksha.v7i1.13009

Abstract

Ammonium concentration in bottled drinking water should not exceed 0.15 ppm. National Standardization Agency of Indonesia (BSN) had issued two standard methods for analysis, Nessler (SNI 01-03554-1998) and Berthelot (SNI 01-03554-2006). Both were statistically compared. The first method was much simpler than the second one because the preparation was only by adding the Nessler reagent into the sample. Whereas in the second one, the addition of reagents should be prepared freshly. The variance coefficient of the first method was 3.41% with linearity 0.9995 and recovery was 101.05%. Whereas variance coefficient of second method was 3.64 % with linearity 0.9995 and recovery was 105.62%. Significance test between the methods showed that value of Fexp (1.043) was less than Fcrit (4.284),  and value of texp (2.36) was less than tcrit  (2.45) in interval of confidence 95%. There was no significant difference between two methods
Co-Authors Adrian Gunawan Adrian Gunawan Bangkit Gotama Bangkit Gotama C Edra Setiawan C Edra Setiawan Dewanto, Hizkia Alpha Endang Saepudin, Endang Fitriyatul Qulub Indah W Utami Indah W Utami Irma Kresnawaty Lumbantobing, Deosdado Farrel H Memik D Pusfitasari Memik D Pusfitasari Mochammad Purwanto Nanik Rahmani NANIK RAHMANI Panji PUSPITA LISDIYANTI Putra Parmita, Ade Wahyu Yusariarta Putra, Ade Wahyu Yusariarta R Wahyu Nugroho R Wahyu Nugroho Rifani, Agus Rizki WAHYU Rusy Dina Sabila Sarastri, Dwi Sarastri, Dwi Sri Sulastri Yopi Yopi Yopi, Yulianto, Kurniawan Yulianto, Kurniawan Yunita Triana, Yunita