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All Journal Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Biosense
Sri Murtiningrum, Sri
RSPAU Dr. S. Hardjolukito, Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health

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Potential of Hibiscus (Hibiscus rosa-sinensis L.) Extract as a Substitute for Eosin in Hematoxylin-Eosin Histological Staining Nadifah, Fitri; Prasetyaningsih, Yuliana; Muhajir, Nurlaili Farida; Murtiningrum, Sri; Ohoiwutun, Clara Shinta D.P; Puspita, Elisabet Della
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.368

Abstract

Staining is an important stage in preparing microscopic preparations so that the histological structure can be observed clearly. Hematoxylin-Eosin is the most widely used dye. Unfortunately, eosin is a synthetic chemical compound that is carcinogenic. To reduce the health and environmental impacts of eosin compounds, it is important to look for alternative dyes from natural ingredients. Hibiscus (Hibiscus rosa-sinensis L.) is a plant known to contain anthocyanins which can be used as eosin substitutes. This study aims to determine the potential of hibiscus extract as a substitute for eosin in histological staining. In this study, unstained liver tissue preparations from mice (Mus musculus) were used. Hibiscus extract dye was compared with eosin as a control. The color quality of the tissue preparations was used as an experimental parameter. The results of the microscopic assessment showed that liver tissue stained with Hibiscus extract at concentrations of 60% and 80% was less good than eosin. However, because tissue can stain reddish, hibiscus extract has the potential to be used as an eosin substitute. The right extraction method may be able to produce intact anthocyanin so that it can color the tissue optimally.
Increase in Neutrophil Lymphocyte Ratio in COVID-19 Patients Based on Symptom Severity Prasetya, Hieronymus Rayi; Muhajir, Nurlaili Farida; Wati, Mey Rena; Murtiningrum, Sri
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.374

Abstract

The neutrophil lymphocyte ratio (NLR) is an indicator of the presence of a systemic inflammatory response. The neutrophil lymphocyte ratio parameter was calculated by comparing the number of neutrophils and the number of lymphocytes. Patients with severe COVID-19 who did not survive were found to have a higher neutrophil lymphocyte ratio compared to patients with a mild prognosis. Thisstudy uses a descriptive method, which aims to describe the value of the neutrophil lymphocyte ratio in COVID-19 patients based on the severity of symptoms. The average value of high neutrophil lymphocyte ratio (NLR) in COVID-19 patients occurred in the severe (6.12%) and critical (10.78%) categories. The average value of high neutrophils (Neutrophilia) in COVID-19 patients occurred in the severe (76%) and critical (82%) categories. The average value of low lymphocytes (lymphocytopenia) in COVID-19 patients occurred in the severe 17% and critical 12% category. The NLR value in patients with the severe category had an increase in the NLR value twice thenormal value, while in the critical category had an increase in the NLR value three times the normal value. 
IDENTIFIKASI JAMUR PENYEBAB TINEA UNGUIUM PADA KUKU KAKI PETANI DI DUSUN BAROS TIRTOHARGO KRETEK BANTUL Nadifah, Fitri; Muhajir, Nurlaili Farida; Sanusi , Kristi Elisabeth; Murtiningrum, Sri; Nuryati, Arum
JURNAL BIOSENSE Vol 8 No 3 (2025): Edisi Juli 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i3.5433

Abstract

Tinea unguium is a type of superficial mycosis that attacks the nail plate as a result of dermatophyte infection. This study aimed to identify the fungal genera responsible for tinea unguium in the toenails of farmers in Baros Hamlet, Tirtohargo Village, Kretek District, Bantul Regency. Thirteen nail samples were examined microscopically with 20 % KOH and Lactophenol Cotton Blue preparations, and cultured on Sabouraud Dextrose Agar (SDA). Three fungal genera were identified: Aspergillus (9 isolates), Penicillium (3 isolates) and Trichophyton (1 isolate). Hence, the dermatophyte causing tinea unguium in these farmers’ feet was Trichophyton sp. Microscopic observations revealed conidiophores, vesicles, phialides, and conidia consistent with the morphological characteristics of each genus. These results underscore the need for education on preventative measures, such as wearing appropriate footwear, maintaining foot hygiene, and increasing farmers’ awareness of the risk of fungal infection.
Co-Authors Arum Nuryati Fitri Nadifah Hieronymus Rayi Prasetya, Hieronymus Rayi Kuncoro Harto Widodo Nurlaili Farida Muhajir, Nurlaili Farida Ohoiwutun, Clara Shinta D.P Puspita, Elisabet Della Sanusi , Kristi Elisabeth Trisasi Lestari, Trisasi Wati, Mey Rena Yuliana Prasetyaningsih