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All Journal Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Biosense Jurnal EduHealth Prosiding Seminar Informasi Kesehatan Nasional
Nurlaili Farida Muhajir, Nurlaili Farida
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Potential of Hibiscus (Hibiscus rosa-sinensis L.) Extract as a Substitute for Eosin in Hematoxylin-Eosin Histological Staining Nadifah, Fitri; Prasetyaningsih, Yuliana; Muhajir, Nurlaili Farida; Murtiningrum, Sri; Ohoiwutun, Clara Shinta D.P; Puspita, Elisabet Della
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.368

Abstract

Staining is an important stage in preparing microscopic preparations so that the histological structure can be observed clearly. Hematoxylin-Eosin is the most widely used dye. Unfortunately, eosin is a synthetic chemical compound that is carcinogenic. To reduce the health and environmental impacts of eosin compounds, it is important to look for alternative dyes from natural ingredients. Hibiscus (Hibiscus rosa-sinensis L.) is a plant known to contain anthocyanins which can be used as eosin substitutes. This study aims to determine the potential of hibiscus extract as a substitute for eosin in histological staining. In this study, unstained liver tissue preparations from mice (Mus musculus) were used. Hibiscus extract dye was compared with eosin as a control. The color quality of the tissue preparations was used as an experimental parameter. The results of the microscopic assessment showed that liver tissue stained with Hibiscus extract at concentrations of 60% and 80% was less good than eosin. However, because tissue can stain reddish, hibiscus extract has the potential to be used as an eosin substitute. The right extraction method may be able to produce intact anthocyanin so that it can color the tissue optimally.
Increase in Neutrophil Lymphocyte Ratio in COVID-19 Patients Based on Symptom Severity Prasetya, Hieronymus Rayi; Muhajir, Nurlaili Farida; Wati, Mey Rena; Murtiningrum, Sri
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.374

Abstract

The neutrophil lymphocyte ratio (NLR) is an indicator of the presence of a systemic inflammatory response. The neutrophil lymphocyte ratio parameter was calculated by comparing the number of neutrophils and the number of lymphocytes. Patients with severe COVID-19 who did not survive were found to have a higher neutrophil lymphocyte ratio compared to patients with a mild prognosis. Thisstudy uses a descriptive method, which aims to describe the value of the neutrophil lymphocyte ratio in COVID-19 patients based on the severity of symptoms. The average value of high neutrophil lymphocyte ratio (NLR) in COVID-19 patients occurred in the severe (6.12%) and critical (10.78%) categories. The average value of high neutrophils (Neutrophilia) in COVID-19 patients occurred in the severe (76%) and critical (82%) categories. The average value of low lymphocytes (lymphocytopenia) in COVID-19 patients occurred in the severe 17% and critical 12% category. The NLR value in patients with the severe category had an increase in the NLR value twice thenormal value, while in the critical category had an increase in the NLR value three times the normal value. 
IDENTIFIKASI JAMUR PENYEBAB TINEA UNGUIUM PADA KUKU KAKI PETANI DI DUSUN BAROS TIRTOHARGO KRETEK BANTUL Nadifah, Fitri; Muhajir, Nurlaili Farida; Sanusi , Kristi Elisabeth; Murtiningrum, Sri; Nuryati, Arum
JURNAL BIOSENSE Vol 8 No 3 (2025): Edisi Juli 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i3.5433

Abstract

Tinea unguium is a type of superficial mycosis that attacks the nail plate as a result of dermatophyte infection. This study aimed to identify the fungal genera responsible for tinea unguium in the toenails of farmers in Baros Hamlet, Tirtohargo Village, Kretek District, Bantul Regency. Thirteen nail samples were examined microscopically with 20 % KOH and Lactophenol Cotton Blue preparations, and cultured on Sabouraud Dextrose Agar (SDA). Three fungal genera were identified: Aspergillus (9 isolates), Penicillium (3 isolates) and Trichophyton (1 isolate). Hence, the dermatophyte causing tinea unguium in these farmers’ feet was Trichophyton sp. Microscopic observations revealed conidiophores, vesicles, phialides, and conidia consistent with the morphological characteristics of each genus. These results underscore the need for education on preventative measures, such as wearing appropriate footwear, maintaining foot hygiene, and increasing farmers’ awareness of the risk of fungal infection.
DAYA HAMBAT EKSTRAK BIJI MANGGA (Mangifera indica L.) TERHADAP PERTUMBUHAN Salmonella typhi Arisandi, Desto; Lesmono, Inez Melani; Hidayat, Tsamrotul Fuadah; Octiria, Nessa; Muhajir, Nurlaili Farida
JURNAL BIOSENSE Vol 8 No 3 (2025): Edisi Juli 2025
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v8i3.5714

Abstract

Resistensi obat dalam pengobatan penyakit infeksi seperti demam tifoid menjadi salah satu tantangan yang dihadapi pada saat ini. Keterediaan bahan alam yang melimpah yang diketahui berpotensi sebagai antibakteri serta perkembangan teknologi berperan penting dalam penemuan berbagai jenis alternatif antibiotik yang dapat digunakan untuk mengatasi penanganan penyakit. Tujuan penelitian yaitu mengetahui kemampuan ekstrak biji mangga untuk menghambat pertumbuhan Salmonella typhi. Jenis penelitian ini yaitu true experiment dengan rancangan posttest only control group design. Penelitian ini dilakukan di laboratorium STIKES Guna Bangsa Yogyakarta pada Juli-Oktober 2023. Metode difusi digunakan untuk menguji aktivitas antibakteri berbagai konsentrasi ekstral biji mangga (20%,40%, 60%, 80%, dan 100%). Kloramfenikol digunakan sebagai kontrol positif terhadap zona hambat Salmonella typhi. Zona hambat pertumbuhan Salmonella typhi pada media Muller Hinton pada konsentrasi 40% (6 mm), 60% (6 mm), 80% (7 mm), dan 100% (8 mm) dengan kekuatan daya hambat pada kategori sedang. Pengujian kontrol positif menggunakan klorampenikol 30 μg menunjukkan adanya zona hambat sebesar 14 mm dengan kekuatan daya hambat pada kategori kuat, sedangkan kontrol negatif menggunakan akuades tidak terdapat adanya zona hambat pertumbuhan bakteri.
STUDI EPIDEMIOLOGI KEJADIAN TUBERULOSIS DI KABUPATEN GUNUNGKIDUL, D.I.YOGYAKARTA Arisandi, Desto; Muhajir, Nurlaili Farida; Islamarida, Rista
Prosiding Seminar Informasi Kesehatan Nasional 2023 : SIKesNas 2023
Publisher : Fakultas Ilmu Kesehatan Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47701/sikenas.vi.2881

Abstract

Indonesia merupakan negara berkembang dengan angka kejadian tuberkulosis (TB) yang tinggi. Pendekatan analisis spasial studi epidemiologi menggunakan Geographic Information System (GIS) dapat digunakan untuk mengetahui besaran masalah kesehatan di suatu wilayah. Tujuan: Mengetahui gambaran penderita TB di wilayah kerja puskesmas, Kabupaten Gunungkidul, D.I.Yogyakarta. Metode: Penelitian bersifat deskriptif dengan pendekatan sistem informasi geografis (SIG). Populasi penelitian yaitu penderita TB yang berasal dari 30 puskesmas yang berada di wilayah Kabupaten Gunungkidul, Yogyakarta pada tahun 2021. Variabel penelitian meliputi: usia, jenis kelamin, klasifikasi berdasarkan lokasi anatomi dan riwayat pengobatan TB, status pekerjaan, dan alamat rumah penderita. Data dianalisis secara deskriptif dan disajikan dalam bentuk pemetaan menggunakan aplikasi ArcView GIS version 3.1. Hasil: Penderita TB sebanyak 138 orang dengan kasus paling banyak terdapat di Kecamatan Wonosari (20,29%%), Kecamatan Ponjong (10,14%) dan Kecamatan Nglipar (9,42%). Mayoritas penderita TB berjenis kelamin laki-laki sebanyak 84 orang (60,87%), usia produktif (15-65 tahun) sebanyak 108 orang (87,26%), TB paru sebanyak 127 orang (92,03%), kasus TB baru sebanyak 124 orang (89,86%), bekerja sebanyak 65 orang (47,10%), dan penderita TB dengan HIV positif (+) terdapat sebanyak 3 orang (2,17%). Kesimpulan: Penderita TB di wilayah kerja puskesmas Kabupaten Gunungkidul, D.I.Yogyakarta lebih banyak terdapat pada laki-laki (60,87%), usia produktif (15-65 tahun) (87,26%), TB paru (92,03%), kasus TB baru (89,86%), bekerja (47,10%).
Stunting Case Study In Grogol Bejiharjo Karangmojo Gunungkidul D. I. Yogyakarta, Indonesia Based On Routine Blood Examination Fadhilah, Siti; Astuti, Dian Wuri; Dewi, Eltanina Ulfameytalia; Muhajir, Nurlaili Farida
Jurnal EduHealth Vol. 13 No. 02 (2022): Jurnal eduHealth, Periode Oktober - December, 2022
Publisher : Sean Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.866 KB)

Abstract

Background: Stunting is defined as the condition of the nutritional status of toddlers who have a height that is classified as less when compared to age. The prevalence rate of stunting under five nationally in 2021 is 24.4%. Objective: The research aims to describe stunting cases in Grogol Bejiharjo Karangmojo Gunung Kidul D.I.Yogyakarta Indonesia based on routine blood tests. Methods: The design of this study used observational analysis with the independent-T test. The number of respondents involved as many as 14 respondents with the purposive sampling method. Results: The number of respondents categorized as stunting based on measurements of height per age (TB/U) was 64.3% consisting of concise and short nutritional status. This number is higher than those who are not stunted (normal), which is 35.7%. The number of leukocytes in the low-stunted group tends to be lower than in the normal (non-stunted) group, as well as the parameters of the lymphocyte count examination that the average result is higher in the stunting group compared to the normal (non-stunted) group. There was a significant difference in the average hemoglobin level between the stunting and non-stunted groups because of the p-value <0.05. A significant difference was also found in the mean hematocrit value between the stunted and non-stunted groups because of the p-value <0.05. Conclusion: statistical differences in hemoglobin levels and hematocrit values between groups. Other blood test parameters were not statistically different.
Co-Authors Arum Nuryati Desto Arisandi Dian Wuri Astuti Eltanina Ulfameytalia Dewi, Eltanina Ulfameytalia Fitri Nadifah Hidayat, Tsamrotul Fuadah Hieronymus Rayi Prasetya, Hieronymus Rayi Lesmono, Inez Melani Maria Theresia Doe Bay, Maria Theresia Doe Octiria, Nessa Ohoiwutun, Clara Shinta D.P Puspita, Elisabet Della Rista Islamarida, Rista Sanusi , Kristi Elisabeth Siti Fadhilah, Siti Sri Murtiningrum, Sri Wati, Mey Rena Yuliana Prasetyaningsih