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Validasi Metode Penetapan Kadar Monosodium Glutamat pada Mie Ayam dengan Spektrofotometri UV–Vis Setelah Derivatisasi Ninhidrin Suprianto; Effendy De Lux Putra; Samran; Siti Nurbaya; Cyndi Veronica; Sumardi; Debi Meilani
Jurnal Indah Sains dan Klinis Vol 6 No 2 (2025): Jurnal Indah Sains dan Klinis
Publisher : Yayasan Penelitian dan Inovasi Sumatera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52622/jisk.v6i2.05

Abstract

Pendahuluan: Mie Ayam merupakan aneka olahan mie yang dijajakan, disajikan dan dinikmati dengan cita rasa yang sangat lezat; dijajakan dari mulai gerobak jalan, di pinggir jalan maupun tempat lesehan. Mie Ayam diolah dengan penambahan berbagai bahan tambahan pangan agar diperoleh makanan yang dapat membangkitkan selera konsumen. Mie Ayam yang disajikan harus diproses dengan benar sehingga konsumen aman setelah konsumsi makanan tersebut. Monosodium glutamate (MSG) merupakan bahan tambahan pangan yang biasa ditambahkan pada makanan untuk meningkatkan cita rasa makanan, terutama rasa lezat makanan. MSG ditambahkan pada produk pangan tidak lebih dari 10g/kg Bahan Pangan agar konsumen terjamin kesehatannya. MSG dapat diderivatisasi dengan ninhidrin sehingga penetapan kadar dapat dilakukan dengan spektrofotometri visibel. Tujuan: Menentukan kadar MSG pada Mie Ayam secara spektrofotometri UV. Metode: Penetapan kadar MSG dilakukan secara spektrofotometri UV setelah dilakukan derivatisasi dengan Ninhidrin. Hasil: Kadar MSG pada Mie diperoleh pada rentang 1,9441- 2,4726 g/kg bahan pangan dan di bawah batas maksimum 10 g /kg bahan pangan menurut EPSA. Kesimpulan: Kadar MSG pada Mie Ayam pada rentang tertentu dan masih di bawah batas maksimum.
Skrining Fitokimia, Profil Kromatografi Lapis Tipis dan Karakterisasi FTIR Ekstrak Etanol Daun Rambutan (Nephelium lappaceum L.) Adinda Seftia; Suprianto; Effendy De Lux Putra; Samran; Siti Nurbaya
Jurnal Indah Sains dan Klinis Vol 6 No 3 (2025): Jurnal Indah Sains dan Klinis
Publisher : Yayasan Penelitian dan Inovasi Sumatera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52622/jisk.v6i3.02

Abstract

Background: Rambutan (Nephelium lappaceum L.) leaves have potential as a source of bioactive secondary metabolites, yet baseline data on their preliminary chemical profile and functional-group features are still limited. Objective: This study aimed to (i) screen the phytochemical constituents of rambutan leaf ethanol extract, (ii) verify major metabolite classes using thin-layer chromatography (TLC), and (iii) characterize dominant functional groups by Fourier Transform Infrared (FTIR) spectroscopy. Method: Dried rambutan leaves collected from Langkat Regency and Binjai City were extracted by maceration with 96% ethanol (1:10; 3×24 h), followed by remaceration (1×24 h). The filtrate was concentrated at 58 °C. Qualitative tests were conducted for alkaloids, flavonoids, tannins, saponins, and steroids. TLC analysis employed silica gel 60 F254 plates with class-specific mobile phases, while FTIR spectra were recorded in the range of 4000–600 cm⁻¹. Results: The extract yield was 25.7%. Phytochemical screening confirmed the presence of alkaloids, flavonoids, tannins, saponins, and steroids, with TLC Rf values of 0.70 and 0.46 (alkaloids), 0.80 (flavonoids), 0.70 (tannins), and 0.50 (steroids). FTIR bands at 3365 cm⁻¹ (O–H), 3010 cm⁻¹ (aromatic C–H), 1629 cm⁻¹ (C=C), and 1048 cm⁻¹ (C–O) indicated oxygenated secondary metabolites, particularly phenolic-related compounds. Conclusion: The integrated phytochemical screening, TLC, and FTIR approach provides a concise chemical baseline for rambutan leaf ethanol extract, supporting its potential use in further standardization and marker-based studies.
PENYULUHAN TENTANG PENCEGAHAN DINI OSTEOPOROSIS KEPADA MASYARAKAT DI KLINIK PRATAMA NUSANTARA KESEHATAN JALAN BAKTI LUHUR NO. 17, MEDAN HELVETIA Cut Masyithah; Artha Yuliana Sianipar; Peri Peri; Siti Nurbaya; Cut Alvida Zura; Rionaldo Agusto Lingga; Eva Diansari Marbun
Jurnal Pengabdian Masyarakat Kesosi Vol. 8 No. 2 (2025): Juli: Jurnal Abdimas KESOSI
Publisher : Sekolah Tinggi Ilmu Kesehatan Kesetiakawanan Sosial Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57213/abdimas.v8i2.328

Abstract

Osteoporosis is a metabolic bone disease characterized by decreased bone mass and microstructure damage, thus increasing the risk of fractures, especially in the elderly. Public knowledge about early prevention of osteoporosis is still relatively low, even though prevention can be done through a healthy lifestyle such as calcium and vitamin D consumption and regular exercise. This counseling aims to improve community knowledge through direct counseling conducted at Nusantara Health Primary Clinic, Medan Helvetia in May 2025. A total of 29 participants attended this activity and were evaluated using a post-test questionnaire. Results showed an average knowledge score of 100%, from the good category. The counseling proved effective in improving the community's understanding of osteoporosis and its prevention. Implementation constraints were due to lack of coordination between us and the community which made the community attend less because some people had posyandu activities carried out on the same day. This counseling is expected to increase the knowledge and awareness of the community at the Pratama Nusantara Health Clinic about the importance of preventing osteoporosis early on.