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All Journal Jurnal Ilmiah Kesehatan (JIKA) Pharmaceutical Sciences and Research (PSR)
Forestrania, Roshamur Cahyan
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Health Professions Medicine & Pharmacology Nursing Public Health

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Inhibitory and Anti-Biofilm Effects of Orthoshipon aristatus Against Candida albicans Rahmasari, Ratika; Chairunissa, Ananda Hanny; Irianti, Marina Ika; Forestrania, Roshamur Cahyan; Arifianti, Ayun Erwina; Suryadi, Herman; Makau, Juliann Nzembi; Jantan, Ibrahim; Elya, Berna
Pharmaceutical Sciences and Research Vol. 7, No. 3
Publisher : UI Scholars Hub

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Abstract

There are increasing number of reports on Candida albicans developing resistance to available anti-fungal drugs. Thus, there is an urgent need to discover new agents for treatment of candidiasis. The alcoholic extracts of Orthoshipon aristatus have been shown to exhibit antifungal activity against C. albicans by using the agar diffusion and broth microdilution methods. However, the underlying mechanisms of anti-C. albicans effect of O. aristatus have not been well understood. This study was aimed to evaluate the cytotoxic and anti-biofilm effects of the n-hexane and ethanol extracts of purple and white varieties of O. aristatus leaves and branches against C. albicans. The effect of n-hexane and ethanol extract against C. albicans growth was carried out by crystal violet viability assay. IC50 values of the most active extract, and nystatin and fluconazole as positive controls were also determined by the crystal violet assay. Evaluation of the anti-biofilm effect was performed by treating C. albicans with the extracts at adhesion, development, and biofilm maturation stages using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The n-hexane extract of the purple variety of O. aristatus leaves demonstrated the strongest cytotoxic activity against C. albicans amongst the tested extracts, with an IC50 value of 0.67 µg/mL. The extract also showed strong anti-biofilm effect as fluconazole, with pronounced inhibition at the adhesion stage and less activity at the biofilm development and maturation stages. These results suggested that the n-hexane extract of the purple variety of O. aristatus leaves could be explored for discovery and development of anti-C. albicans agent.
Characterization of Phytocomponents of Mindi Leaf Extract and Chinese Petai Seeds by LC-HRMS and In Silico Activity Against Alpha-glucosidase Maharani, Maharani; Elya, Berna; Forestrania, Roshamur Cahyan
Jurnal Ilmiah Kesehatan (JIKA) Vol. 6 No. 2 (2024): Volume 6 Nomor 2 Agustus 2024
Publisher : Sarana Ilmu Indonesia (salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v6i2.1007

Abstract

Melia azedarach L. and Leucaena leucocephala L. were two plant species that had attracted attention in phytochemical research due to their bioactive potential. However, research on the phytochemical characterization using Liquid Chromatography – High-Resolution Mass Spectrometry (LC-HRMS) of these two plants was still limited. This study was to identify and characterize the phytochemical properties of Mindi leaves (Melia azedarach L.) and Chinese Petai seeds (Leucaena leucocephala L.), as well as to conduct an in silico study. The research method involved reflux extraction using 70% ethanol as the solvent, qualitative phytochemical identification, and characterization using LC-HRMS. The compounds from LC-HRMS from each extract were analyzed for their bioactivity in silico. The results showed that Mindi leaves (M. azedarach L.) and  Petai cina seeds (L. leucochepala L.) contain alkaloids, saponins, phenolics, flavonoids, and tannins. The results of LC-HRMS analysis showed that 10 compounds were identified in each extract, and 3 of them were Prolylleucine, (3beta, 24R, 24'R)-fucosterol epoxide, and 2,3-Dihydro-1-benzofuran-2-carboxylic acid that is a compound found in both extracts. In silico results show that there is 1 compound in mindi leaf extract, namely Methyl isonicotinic, and 3 compounds from petai cina seed extract, namely trans-p-Coumaraldehyde, L-alanine, and 3-Buten-1-amine, which show pretty strong alpha-glucosidase inhibitory activity inhibits the target protein 3A4A. This research can be a reference for developing mini leaf extract and petai cina seeds as anti-diabetic candidates.
Co-Authors Arifianti, Ayun Erwina Berna Elya Chairunissa, Ananda Hanny Herman Suryadi Irianti, Marina Ika Jantan, Ibrahim Maharani Maharani, Maharani Makau, Juliann Nzembi Rahmasari, Ratika