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Potency of Kaliasem Bark (Syzygium polychephalum) Extract as Antibacterial Agent for Staphylococcus aureus Ni Luh Putu Agustini; Desak Putu Risky Vidika Apriyanthi; Ayu Saka Laksmita
Jurnal Biologi Tropis Vol. 22 No. 1 (2022): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i1.2967

Abstract

Nosocomial infectious diseases are caused by Staphylococcus aureus bacteria. Treatment of infection can use drugs in the form of substances produced by a microorganism in the form of antibiotics. However, the problem that often arises with antibiotics is the occurrence of resistance. Alternatives that are able to overcome the problem of antibiotic resistance against bacteria are plants that have antibacterial activity, one of which is kaliasem (Syzygium polychephalum). This study aims to determine the compounds contained in the ethanol extract of the bark of kaliasem (Syzygium polychephalum) and to determine the potential of its activitiy on the growth of Staphylococcus aureus in vitro. The bark extract of kaliasem (Syzygium polychephalum) had been carried out by using maceration method. Determination of antibacterial activity was carried out by the disc diffusion method. The ethanolic extract of the bark of kaliasem (Syzygium polychephalum) contains antibacterial compounds such as tannins, phenolics, terpenoids, alkaloids and flavonoids and the ethanolic extract of the bark of kaliasem (Syzygium polychephalum) at concentrations of 25%, 50%, 75% and 100% can inhibit growth of Staphylococcus aureus bacteria with an average inhibition zone of 15.85 mm, 18.36 mm, 23.64 mm, 28.58 mm, respectively. Therefore, it can be concluded that the ethanolic extract of the bark of kaliasem (Syzygium polychephalum) contains antibacterial compounds and has the potential to inhibit the growth of Staphylococcus aureus bacteria.
Lipid Peroxidation Inhibition Activity of Sintrong (Crassocephalum crepidioi-des) Leaf Extract in Rats Consuming Arak Jembrana Widayanti, Ni Putu; Laksmita W, Ayu Saka; Apriyanthi, Desak Putu Risky Vidika; Arijana, I G K Nyoman
Makara Journal of Science Vol. 24, No. 4
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The present study was conducted to determine the antioxidant activity of sintrong (Crassocephalum crepidioides) leaf extract to inhibiting lipid peroxidation by decreased malondialdehyde (MDA) level in the blood plasma of rats consuming arak Jembrana. The process of making sintrong leaf extract used 96% ethanol with maceration techniques. In vivo testing used five groups: (1) a control group, (2) treatment with arak, (3) treatment with arak followed by extract at a dose of 37.5 mg/kgBW, (4) treatment with arak followed by extract at a dose of 50 mg/kgBW, and (5) treatment with arak followed by extract at a dose of 75 mg/kgBW. As much as 1 cc/day of arak was provided for seven days. The extract treatment at a dose of 37.5 mg/kgBW decreased the MDA level from 21.24 nmol/L to 16.33 nmol/L. Based on a Tukey’s Honestly Significant Difference (HSDa) test, this treatment was not significantly different from that of the control group (normal condition).
PEMERIKSAAN STATUS KESEHATAN BODY MASS INDEX (BMI) DAN KADAR GLUKOSA DARAH DI SMA NEGERI BALI MANDARA Ni Putu Rahayu Artini; Desak Putu Risky Vidika; Ayu Saka Laksmita; I Wayan Tanjung Aryasa; Ni Putu Widayanti; Yulidia Iriani L.
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 5, No 8 (2022): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v5i8.2828-2833

Abstract

Indonesia merupakan salah satu negara berkembang yang mengalami masalah gizi double burden, yaitu underweight dan overweight. Masalah gizi dapat meningkatkan risiko penyakit rendah BMI seperti malnutrisi, hipoglikemia dan hipotensi. Sedangkan penyakit yang dapat disebabkan karena tingginya BMI dapat berupa obesitas, hiperglikemia, hiperkolesterol, hipertensi dan penyakit jantung koroner. Pemeriksaan tersebut dapat dilakukan melalui pemeriksaan gratis pengabdian masyarakat yang dilakukan oleh Program Studi Teknologi Laboratorium Medik, Universitas Bali Internasional dengan sasaran pada siswa, staf dan guru di SMA Negeri Bali Mandara. Pemeriksaan ini menggunakan metode point of care testing (POCT). Hasil pemeriksaan kesehatan siswa SMA Negeri Bali Mandara yang memiliki BMI < 18,5 dengan persentase 13,34%, BMI 18,5-24,9 sebanyak 80% kategori ideal, 6,66% memiliki BMI berlebih. Sedangkan untuk staf dan guru SMA Negeri Bali Mandara 1 orang memiliki kriteria BMI kurang ideal dengan persentase 2,38%,  BMI ideal dengan persentase 45,23%,  BMI berlebih dengan persentase 40,47% dan BMI kriteria obesitas dengan persentase 11,92%. Berdasarkan parameter kadar glukosa darah, semua siswa SMA Negeri Bali Mandara memiliki kadar glukosa normal. Sedangkan kadar glukosa staf dan guru SMA Negeri Bali Mandara hanya 4,77% yang memiliki kadar glukosa tinggi. Kesimpulan dari kegiatan pengabdian masyarakat tentang pemeriksaan BMI dan glukosa darah dapat digunakan untuk pengecekan status kesehatan.
Community Knowledge, Attitudes and Behaviors towards Obesity Prone to COVID-19 Infection Ayu Saka Laksmita; Desak Putu Risky Vidika Apriyanthi; Ni Putu Widayanti
Disease Prevention and Public Health Journal Vol. 17 No. 1 (2023): Disease Prevention and Public Health Journal
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/dpphj.v17i1.6968

Abstract

Background: The COVID-19 pandemic is an infectious disease that has spread worldwide. Prevention of COVID-19 can be suppressed with a good level of knowledge and positive attitudes and behaviors. The community needs to play a role in breaking the chain of transmission of COVID-19 by implementing health protocols. The purpose of this study was to determine the relationship between the level of public knowledge about COVID-19 and the attitudes and behavior of the people of Denpasar City towards obesity, which are more susceptible to infection with COVID-19. Method: This type of non-experimental research with correlational design and cross-sectional design. The number of samples in this study was 400 respondents using simple random sampling. Data collection uses a questionnaire via WhatsApp with a google form. Results: The results showed that there was a relationship between public knowledge about COVID-19 and the attitudes and behavior of the people of Denpasar City towards obesity being more susceptible to infection with COVID-19 with p = 0.000 and r = 0.307 for attitudes and p = 0.000 and r = 0.408 for behavior. Conclusion: A strong correlation between the variable level of knowledge and attitudes and behavior and the direction of the relationship is positive, which means the better the level of knowledge, the better the attitude and behavior of the community to prevent contracting COVID-19.
Pemeriksaan Kesehatan (Kadar Gula Sewaktu dan Asam Urat) bagi Pengungsi Bencana Gempa Bumi di Desa Ban Kecamatan Kubu Kabupaten Karangasem Bali Desak Putu Risky Vidika Apriyanthi; Ayu Saka Laksmita W
Bubungan Tinggi: Jurnal Pengabdian Masyarakat Vol 5, No 1 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/btjpm.v5i1.7509

Abstract

Gempa bumi yang berkuatan 4,8 skala richter (SR) terjadi di Desa Ban, Kecamatan Kubu, Kabupaten Karangasem Bali. Hal ini berdampak pada segala sektor, khususnya pada kesehatan pengungsi. Mayoritas pengungsi mengalami gejala seperti pusing, sakit kepala, nyeri badan, susah tidur, dan gatal-gatal. Kondisi kesehatan lingkungan yang buruk di tempat pengungsian perlu ditanggulangi agar tidak menyebabkan penyebaran penyakit yang berbahaya. Kegiatan Pengabdian kepada Masyarakat (PkM) yang dilaksanakan pada 16 Desember 2021 di Balai Desa Ban bertujuan untuk membantu para pengungsi Desa Ban dalam skrining kesehatan yang meliputi pemeriksaan gula darah sewaktu dan asam urat. Kegiatan ini diharapkan dapat mengetahui potensi resiko penyakit kronis, meliputi Diabetes Mellitus (DM) dan asam urat sedini mungkin sehingga dapat mencegah terjadinya penyakit degeneratif. Kegiatan pengabdian ini diikuti oleh 80 orang pengungsi. Kegiatan ini merupakan kolaborasi antar Program Studi di lingkungan Fakultas Ilmu-ilmu Kesehatan Universitas Bali Internasional. Pemeriksaan kesehatan dilakukan dengan registrasi peserta kemudian seluruh peserta diperiksa gula darahnya dan beberapa orang yang memiliki keluhan nyeri sendi diarahkan untuk melakukan pemeriksaan asam urat. Dari hasil pemeriksaan kesehatan didapatkan bahwa sebagian besar pengungsi di Desa Ban kadar gula sewaktu yang masuk dalam kategori prediabetes dan memiliki kadar asam urat dalam kategori normal. Pemeriksaan kesehatan berlangsung lancar dan sangat didukung oleh aparat Desa. Kegiatan ini diharapkan dapat dilakukan secara berkala untuk meningkatkan derajat kesehatan para pengungsi yang terdampak bencana gempa bumi.An earthquake measuring 4.8 on the Richter Scale (SR) occurred in Ban Village, Kubu District, Karangasem Regency, Bali. This had an impact on all sectors, especially on the health of the evacuees. Most evacuees experienced dizziness, headaches, body aches, insomnia, and itching. Poor environmental health conditions in refugee camps need to be addressed so as not to cause the spread of dangerous diseases. The community service activity on December 16, 2021, at Ban Village Hall aims to assist Ban Village refugees in health screening, including checking blood sugar and uric acid. This activity is expected to determine the potential risk of chronic diseases, including Diabetes Mellitus (DM) and uric acid, as early as possible to prevent degenerative diseases. Eighty refugees attended this community service activity. This activity is a collaboration between Study Programs within the Faculty of Health Sciences, Bali International University. The health examination was carried out by registering participants. All participants were checked for blood sugar, and some people complained of joint pain were directed to do a uric acid test. From the health check results, it was found that most of the refugees in Desa Ban had blood sugar levels that fell into the prediabetes category and uric acid levels in the normal category. The health checks went smoothly and were strongly supported by the village officials. This activity is expected to be carried out regularly to improve the health status of refugees affected by the earthquake. 
Perbandingan Kadar Air, Asam Lemak Bebas dan Bilangan Peroksida Pada Minyak Curah dan Minyak Tandusan di Desa Baluk, Jembrana Ni Putu Widayanti; Ayu Saka Laksmita W; Desak Putu Risky Vidika Apriyanthi
Jurnal Ilmiah Teknologi Pertanian Agrotechno Vol 8 No 1 (2023)
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JITPA/2023.v08.i01.p08

Abstract

The scarcity of cooking oil in various regions is a major problem in the food security sector. One alternative that can be done to overcome this phenomenon is to develop tandusan oil as a product of local wisdom in Baluk Village, Jembrana. The purpose of this study was to determine the water content, free fatty acids, and peroxide number of the tandusan oil and compare it with the bulk oil on the market. Testing the oil quality parameters each uses three samples with the AOAC (Association of Official Analytical Chemists) method. The test results from this study obtained that the water content in the three samples of tandusan oil was 0.16%; 0.12%; and 0.14% while in the bulk oil sample it was 0.47%; 0.45%; and 0.48%. The free fatty acid content obtained from the tandusan oil test was 0.49%; 0.42%; and 0.46% while in the bulk oil sample it was 2.39%; 2.41%; and 2.45%. The measured peroxide number in the tandusan oil was 0.52 mg eq/kg; 0.55 mg eq/kg; and 0.50 mg eq/kg whereas in the bulk oil it is 3.49 mg eq/kg; 3.55 mg eq/kg; and 3.59 mg eq/kg. Tandusan oil has better oil quality than bulk oil and meets SNI 2902:2011 and SNI 3741: 2013 so it is safe to use as food.
AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN TULAK (Schefflera elliptica (Blume) Harms) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli: Antibacterial Activity of Ethanol Extract of Tulak Leaves (Schefflera elliptica (Blume) Harms) Against Staphylococcus aureus and Escherichia coli Ni Putu Saraswati Kristina; Aryasa, I Wayan Tanjung; Desak Putu Risky Vidika Apriyanthi
Jurnal Tumbuhan Obat Indonesia Vol. 16 No. 1 (2023): July 2023
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v16i1.601

Abstract

Tulak plant is a type of plant that commonly used as a traditional ceremonies and traditional medicine. The purpose of this study was to determine the content of secondary metabolites in tulak leaves (Schefflera elliptica (Blume) Harms) and antibacterial activity against Staphylococcus aureus and Escherichia coli. The extraction method used is the maceration method and for the antibacterial test using the well method. The results of the phytochemical screening test on tulak leaves contain saponins, tannins, phenols and alkaloids. The results of the antibacterial activity test against Staphylococcus aureus at concentrations of 25%, 50%, 75% and 100% respectively had inhibition zones of 6.10±0.05 mm, 7.15±0.03 mm, 8.12±0 .04 mm, and 9.07 ± 0.05 mm which are categorized as moderate. The results of the antibacterial activity test against Escherichia coli at concentrations of 25%, 50%, 75% and 100% respectively did not show the formation of an inhibition zone so value was 0.00±0.00 mm. The conclusion from this study is that tulak leaves (Schefflera elliptica (Blume) Harms) have antibacterial activity against Staphylococcus aureus but do not have antibacterial activity against Escherichia coli.
UJI FITOKIMIA DAN ANTIBAKTERI FRAKSI AIR KULIT BATANG TANAMAN TURI (Sesbania grandiflora L.) TERHADAP BAKTERI Staphylococcus aureus Ni Luh Made Rahayu Widya Lestari; Desak Putu Risky Vidika Apriyanthi; Ni Putu Rahayu Artini
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.14515

Abstract

Staphylococcus aureus is a bacteria that can cause skin and soft tissue infections such as urinary tract infections, pneumonia, mastitis, and meningitis. Staphylococcus aureus infections are usually treated with antibiotics, but most antibiotics are resistant to the bacteria and have side effects of toxicity to the body. One alternative to finding new antibacterials from natural ingredients is to use turi plants. This study aims to determine the content of secondary metabolites in the water fraction of the bark of turi (Sesbania grandiflora L.) and to determine the antibacterial activity of the water fraction of the bark of turi (Sesbania grandiflora L.) against Staphylococcus aureus. This research is an experimental study, using the bark fraction of the turi plant. The fraction was obtained after the fractionation process from the bark extract of turi (Sesbania grandiflora L.) so that the water fraction was divided into several concentrations, namely 25%, 50%, 75%, and 100%. Antibacterial activity test was carried out by well diffusion method. The results of this study indicate that the water fraction of the bark of turi (Sesbania grandiflora L.) contains secondary metabolites of alkaloids, tannins, saponins, steroids, and phenolics. The antibacterial activity produced was included in the moderate category at a concentration of 25% with an inhibition zone of 9.70 ± 0.14 mm, a strong category at concentration of 50% and 75% with an inhibition zone of 12.37 ± 0.25 mm, and 18.47 ± 0.23 mm, in addition to the category of very strong at a concentration of 100% with an inhibition zone of 23.47 ± 0.09 mm.
Identification of Microplastics in Euthynnus affinis in Kedonganan Area, Kuta, Badung, Bali Ayu Saka Laksmita W; Desak Putu Risky Vidika Apriyanthi; Ni Putu Widayanti
Jurnal Ilmu Lingkungan Vol 22, No 5 (2024): September 2024
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.22.5.1293-1298

Abstract

Plastic waste pollution in the ocean can compromise food safety for the community in the world. One of the most dangerous polution today is microplastics. Microplastics are plastic particles that are ≤ 5 μm in size and cannot dissolve in water. Fish is one of the important foodstuffs that is often consumed by humans because, besides its delicious taste, fish also has good nutritional value. The presence of microplastic contamination in fish consumed by humans will certainly be very detrimental to humans. The purpose of this study was to determine the type and abundance of microplastics in fish Euthynnus affinis in the Kedonganan area, Kuta, Badung, Bali. This study was conducted from April to May 2023. The fish samples used were 30 samples with descriptive qualitative analysis. Microplastic particles were extracted first for further analysis. The results showed the types of microplastics found in Euthynnus affinis, namely film, fibre, and fragment types. While the abundance of microplastics in Euthynnus affinis, the fibre type is the most common type of microplastics found at 2.1 particles/individual followed by fragment and film types at 1.1 particles/individual and 0.8 particles/individual respectively.
EXAMINATION OF SOIL-TRANSMITTED HELMINTHS (STH) IN THE FINGERNAILS AND TOENAILS OF SCAVENGERS AT SUWUNG LANDFILL. Apriyanthi, Desak Putu Risky Vidika; W, Ayu Saka Laksmita; Widayanti, Ni Putu
BioCONCETTA Vol 9, No 2 (2023)
Publisher : Universitas PGRI Sumatera Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22202/bc.2023.v9i2.7721

Abstract

Scavengers are individuals who gather used items or specific waste for recycling. Scavenging work has resulted in the notion that the scavenger's way of life is an unhealthy lifestyle. The environment and workplace are not conducive and are unclean, so there is a high possibility that scavengers can contract various illnesses, such as coughing, itching, diarrhea, and others. Risk factors for STH worms entering the human body are poor personal hygiene, which can be caused by not being familiar with maintaining proper hand and nail hygiene. Fingernails become a place where worm eggs are attached and can be swallowed when eaten. The purpose of this study was to determine the presence of STH (Soil-Transmitted Helminth) eggs on the fingernails and toenails of scavengers in Suwung landfill. The research design was carried out with an observational study using the cross-sectional method and the results were analyzed with Chi-Square which was carried out by assessing the relationship between the presence of STH eggs and the use of PPE while working. A total of 10 respondents were sampled for their toenails and hands. The results revealed an incidence of STH egg infection in 20% of fingernails and 10% of toenails caused by Ascaris lumbricoides and Trichuris trichiura eggs. A Chi-Square test was performed to examine the correlation between the usage of PPE and the occurrence of STH infection, and a p-value> 0.05 was obtained. Therefore, it can be concluded that there is no strong relationship between both of them.