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All Journal Natural Science: Journal of Science and Technology Biocelebes Jurnal Kedokteran Universitas Palangka Raya Edubiotik : Jurnal Pendidikan, Biologi dan Terapan

Orryani Lambui

Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam UniversitasTadulako

Author-ID : 542547

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Public Health

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The Growth Of White Oyster Mushroom Mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) From Liquid And Solid Inoculum Nisfaun Safitriana; Umrah; Orryani Lambui
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

The study about the growth of white oyster mushroom mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) from liquid and solid inoculum conducted on January until July 2018 in Biotechnology laboratory, Biology Department, Faculty of Math and Science, Tadulako University. This study was aimed to find out the mycelium growth of source of stem inoculum and hood in liquid and solid media. This study was designed by Complete Randomized Design (CRD) which consisted of four treatments and five replications. The treatment were M1 (source of stem inoculum in liquid medium), M2 (source of stem inoculum in solid medium), M3 (source of hood inoculum in liquid medium), and M4 (source of hood inoculum in solid medium). The parameters were (a) incubation time till mycelium fills medium, (b) CFU, and (c) the viability of inoculum on producing medium. The result showed that the faster incubation were M1 and M3 (for 2 days, higher CFU was M3 with an average number 8,2 x 10 10 CFU /ml. The faster viability of mycelium growth were M3 with an average growth of mycelium 6,97 cm/days.

Formulation of Inoculum a white oyster Mushroom (Pleurotus ostreatus) on powder Khadijah; Umrah; Orryani Lambui
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

The study about the formulation of inoculum a white oyster mushroom (Pleurotus ostreatus) on flour. was conducted at Biotechnology laboratory of Departement , Faculty of Mathematic and Natural Science, Tadulako University. This study were aimed to oyster mushroom on production. on and to abridge the cycle of oyster mushroom production process of white.peplication used Compeletely Randomized Design (CRD) consist of six treatments and tree by comparing between (sawduts,rice,bran, and comflour) P1(50% : 27% : 23% , P2 (50% : 30% : 20%), P3 (50% : 33% : 17%), P4 (50% : 36% : 14% ), P5 (50% : 39% : 11%), P6 (50% : 42% : 8%). There are four parameters used in this study (1) .The growth of white oyster mushroom inoculum (2) the incubation time. and, (3) coloni forming unit (CFU), (4) Viability test .The resulth showed that the best growth mycelium was 1,3cm/days with incubation period was 26 days, the highest total of CFU in P5 was 12,7x 108 CFU/ml and was in P3 was 3,3 x 108 CFU/ml .The inoculum viability test of production media in P5, was faster in incubation period is 18 days.

STUDI ETNOBOTANI TUMBUHAN OBAT MASYARAKAT SUKU TOPO UMA DI DESA BERDIKARI KABUPATEN SIGI SULAWESI TENGAH Alvionita Alvionita; Orryani Lambui; Ramadanil Pitopang
Biocelebes Vol. 14 No. 2 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i2.15261

A research entitled Ethnobotanical Studies of Medicinal Plant of Topo Uma Tribe in Berdikari Village Sigi Regency Central Sulawesi has been carried out from December 2016 to February 2017. The research objective was to obtain species of plants used by community of Topo Uma tribe. This research used qualitative and quantitative method. The result showed that there were 56 plants species from 30 families that used by Topo Uma Tribe in Berdikari village. The highest percentace that use in the part of plants were 56,33% of leaves. The processing method is crushed, roasted, pulverized, fried, baked, shredded, boiled, brewed, boiled made cigarette, chewed, cooked as a vegetable, direct attached parts of the body. Types of diseases that are treatable infectious diseases, non-communicable diseases and health care.

UJI DAYA HAMBAT EKSTRAK DAUN CENGKEH (Syzygium aromaticum (L.) Merr. & L. M Perry.) TERHADAP PERTUMBUHAN BAKTERI Shigella dysenteriae Iis Salihat; Orryani Lambui; Ramadanil Pitopang
Biocelebes Vol. 14 No. 2 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i2.15263

Research about the inhibitory test of Syzygium aromaticum leaf extract on the growth of Shigella dysenteriae bacteria which aims to determine the effectiveness of S. aromaticum leaf extract in inhibited the growth of S. dysenteriae bacteria, knowing the concentration of leaf extract is effective in inhibited the growth of S. dysenteriae bacteria and the content of flavonoid compounds, saponins, tannins and alkaloids contained in leaves S. aromaticum, has been conducted from July until December 2016. Extraction method used is Maseration method. Inhibitory test extract on S. dysenteriae bacteria using disc diffusion method. This research was arranged in Completely Randomized Design with 6 treatments and 3 repetitions with extract concentration 30%, 45%, 60% and 75% and 2% Cotrimoxazole antibiotics as positive controls and Aquades as negative controls. The results showed that 75% extract concentration of S. aromaticum plant produced the largest drag zone compared to other concentrations of 17 mm. This indicates that S. aromaticum leaf extract has good inhibitory ability against S. dysenteriae bacteria.

EKSPLORASI BAKTERI LOKAL PENGHASIL ANTIMIKROBA DI CAGAR ALAM TANJUNG API TELUK TOMINI SULAWESI TENGAH, INDONESIA Alwi Millang; Widiawati; Umrah; Orryani Lambui
Biocelebes Vol. 15 No. 1 (2021)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v15i1.15472

ABSTRACT Tanjung Api Nature Reserve is a headland located in Tomini Bay Ampana District Tojo Una-Una Regency, Central Sulawesi Province. This study aims to obtain local bacteria isolates as antimicrobial producers. The method used in this research is purposive sampling and screening method using "Agar Dua Lapis" (Double Layer Method). Testing antimicrobial potency using the method to make Well Diffusion (well) using three pathogens of microbial tests (S. aureus, E. coli, and C. albicans). In each well hole is given 500 μL of bacterial isolates each and incubated at a temperature of 37 oC for 24 hours and continued for 48 hours. The observed parameter is a clear zone formed around the well. Antimicrobial-producing bacteria screening results. obtained as many as 24 isolates of bacteria. Testing of antimicrobial potency by looking at the resulting inhibitory zone is obtained five isolates namely S2U1, S3U3, S7U3, S9U3, and S10U3 are the highest isolates of the bland zone of 12.25 mm but only against S. aureus, while S9U3 is the best isolate because it is able to inhibit the four test microbes namely S. aureus by 5.50 mm, E. coli 9.25 mm, and C. albicans 5.50 mm. Keywords: Exploration of bacteria, antimicrobials, and Nature Reserve

PERBANDINGAN JUMLAH BAKTERI TANAH PADA HUTAN BERDAUN LEBAR DAN HUTAN BERDAUN JARUM PADA KEDALAMAN TANAH YANG BERBEDA Abi Gael Sidon; Wahyu Harso; Umrah Umrah; Orryani Lambui
Biocelebes Vol. 17 No. 1 (2023)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v17i1.16439

The number of soil microorganisms that play a role in the organic matter decomposition process can be affected by litter quantity and quality. Lignin content is higher in needleleaf than in broadleaf. The study aimed to determine the number of bacteria found in the broadleaf forest and needleleaf forest soil at different soil depths. The soil was collected at depths of 0-5 cm, 10-15 cm, and 20-25 cm at a distance of 1.5 m from the canopy of both broadleaf and coniferous forests where their locations are close. The results showed that the soil organic matter of broadleaf forests was higher than needleleaf forests, especially at depths of 0-5 cm and 10-15 cm. The number of bacteria on broadleaf forest soil is higher than on needleleaf forest soil, especially at 0-5 cm depth. The organic matter in both forest soils decreases with the soil depth. A positive correlation is obtained between the quantity of organic matter and the number of bacteria. The quantity and quality of organic matter in the soil will affect the number of bacteria.

Co-Authors Abi Gael Sidon Alvionita Alvionita Alwi Millang Andi Aldi Matoro Putra Ardiputra, Muh.Akbar Bidayanti Ruru Cindy Claudia, Cindy Fitri Maija Handayani, Mutia I Nengah Suwastika Iis Salihat Imanuel , Natasya Nadya Imelda, Silvia Is Patuh Hallianah Khadijah Kurniawan P. Bandjolu LANGKAH SEMBIRING Magfirahtul Jannah Marina Marina Melsi Mengkido Musjaya M. Guli Mutmainnah Mutmainnah Nisfaun Safitriana Nunung Safriana Nur Afiyah Labambe Prismawiryanti Prismawiryanti Priyandini, Nabila Ramadanil Pitopang Ramadhanil pitopang Rosidah, Maya Sofiyanti S. Rahayu, S. Sa’adia Alydrus Suwitra, I Ketut Talantan, Vrillia Marannu Toemon , Agnes Immanuela Umrah Umrah Umrah Umrah Wahyu Harso Widiawati Yunus, Fahrunnisa

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