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Nurlaili Humaidah, Nurlaili
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Peningkatan Kompetensi Guru-Guru SMK Jawa Timur dalam Bidang Aplikasi Mikrobiologi Industri Guna Pendidikan Berkualitas dan Berkelanjutan Altway, Saidah; Zuchrillah, Daril Ridho; Suprapto, Suprapto; Qomariyah, Lailatul; Prajitno, Danawati Hari; Puspita, Niniek Fajar; Soeprijanto, Soeprijanto; Pudjiastuti, Lily; Humaidah, Nurlaili
Sewagati Vol 9 No 4 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i4.2358

Abstract

Mikrobiologi memiliki peran penting dalam berbagai sektor industri, mulai dari pangan, farmasi, hingga bioteknologi. Mikroorganisme berkontribusi signifikan dalam proses fermentasi untuk produksi makanan dan minuman. Selain itu, peran dalam sektor pengolahan limbah, mikroorganisme digunakan untuk bioremediasi, yaitu penguraian limbah dan polutan menjadi bahan yang tidak berbahaya, mendukung praktik industri yang lebih berkelanjutan. Dengan berkembangnya pemanfaatan mikroorganisme dalam dunia industri, Departemen Teknik Kimia Industri melakukan pengabdian masyarakatya dengan mengadakan pelatihan aplikasi mikrobiologi kepada guru-guru SMK bidang teknik kimia seluruh Jawa Timur yang merupakan sebagai upaya meningkatkan kompetensi kepada guru-guru SMK bidang teknik kimia di Jawa Timur. Pelatihan ini telah berlangsung 17 Juli – 28 Agustus 2024 yang berkolaborasi dengan praktisi industri biodiesel serta ditutup dengan mengunjungi beberapa SMK di Jawa Timur. Kegiatan pengabdian masyarakat ini berhasil ditandai dengan peningkatan pemahaman para peserta dan keikutsertaan peserta dalam melaksanakan akutualisasi diri.
Isolation and characterization of docosahexaenoic acid producing Thraustochytrids from mangrove ecosystem Humaidah, Nurlaili; Muslichatin, Wirdhatul; Setyawati, Dyah; Puspita, Niniek Fajar; Nakai, Satoshi; Purwasih, Sasanti Bhekti; Putri, Nurhalida Listiani
Konversi Vol 14, No 1 (2025): APRIL 2025
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/k.v14i1.22114

Abstract

Polyunsaturated Fatty Acids (PUFAs) are unsaturated fatty acids that include DHA fatty acids. The conventional source of DHA is derived from various species of fish in the form of fish oil. DHA content in fish oil is relatively low, ranging from 7-14%, which necessitates the use of large quantities of fish, potentially leading to overfishing. Alternative source of DHA can be obtained through the isolation of thraustochytrids. Thraustochytrids can produce relatively higher levels of DHA and does not require extensive land areas for cultivation. This study aims to identify applicable techniques for isolating and identifying Thraustochytrid strains local to mangrove areas in East Java, `to examine the growth and general fatty acid profile, and to identify the most effective methods for controlling contamination during Thraustochytrid growth. The isolation involved several steps, including sample preparation, isolation, pure culture development, and media control by adjusting pH and temperature. Visual observations were made using an inverted microscope to confirm the presence of Thraustochytrids followed by genetic analysis to identify the strain. The optimal growth was observed in L3P sample collected from brown mangrove leaves. The highest specific growth rate was 0.146 h-1 (L3P). DHA content was 172 mg/g from the dried cell weight biomass. The most effective method for isolation involved a pre-treatment step consisting of soaking the samples in pine pollen for 8-10 days. Thraustochytrid isolates were able to grow in media with a pH of 7 and at a temperature of 25°C.
Co-Authors Adi Soeprijanto Ali Altway Danawati Hari Prajitno Dyah Setyawati Lailatul Qomariyah, Lailatul Lily Pudjiastuti, Lily Muslichatin, Wirdhatul Nakai, Satoshi Niniek Fajar Puspita, Niniek Fajar Purwasih, Sasanti Bhekti Putri, Nurhalida Listiani Suprapto Suprapto Zuchrillah, Daril Ridho