Mamik Setyowati, Mamik
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Ragunan 29, Pasar Minggu, Jakarta Selatan 12540, Indonesia

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Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques Susilowati, Dwi Ningsih; Fauziah, Fani; Pranoto, Eko; Hidayat, Ernin; Setyowati, Mamik; Rachmiati, Yati
Jurnal Penelitian Teh dan Kina Vol 19, No 2 (2016)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.744 KB) | DOI: 10.22302/pptk.jur.jptk.v19i2.114

Abstract

Bio-imunizer contains an active compound of  Chryseobacterium sp. and Bacillus sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is Terminal Restriction Fragment Length Polymorphism based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielous evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that Chryseobacterium sp. and Bacillus sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.
HUBUNGAN KARAKTER FENOTIPIK DAN HASIL BIJI PLASMA NUTFAH KACANG TUNGGAK [Vigna unguiculata (L.) Walp.] MENURUT ANALISIS LINTASAN Mastur, Mastur; Setyowati, Mamik; Susilowati, Dwi N.
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4495.35 KB) | DOI: 10.14203/beritabiologi.v17i2.2262

Abstract

Selection of high quality seedling through plant breeding activity can be conducted using yielded seeds, but usually it produces a low heritability. Therefore, this study was aimed to examine and analize several secondary phenotypic characters of 115 cowpea germ plasms in relation to seed yield. Relationship between secondary characters and seed yield was analyzed using path analysis. The result of path analysis showed that the number of cluster and the number of pod exhibited direct and significant effect to the seed yield of cowpea. Therefore, number of cluster and number of pod are necessary to be included as the selection criteria for breeding programme to produce high quality cowpea seedling with high yield.
ANALISIS AKTIVITAS NITROGENASE DAN GEN NIFH ISOLAT BAKTERI RHIZOSFER TANAMAN PADI DARI LAHAN SAWAH PESISIR JAWA BARAT Susilowati, Dwi Ningsih; Setyowati, Mamik
Al-Kauniyah: Jurnal Biologi Vol 9, No 2 (2016): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.064 KB) | DOI: 10.15408/kauniyah.v9i2.4036

Abstract

AbstrakPenambatan nitrogen oleh bakteri rhizosfer dapat dimanfaatkan untuk menyiasati dampak salinitas pada tanah sawah pesisir. Kemampuan tersebut disebabkan oleh aktivitas nitrogenase yang disandikan gen nifH pada komponen II. Penelitian  ini bertujuan  menganalisis aktivitas nitrogenase pada kondisi salin dan mengidentifikasi gen nifH. Sebanyak 50 isolat bakteri rhizosfer asal tanah sawah pesisir daerah Eretan dan Patimban, Jawa Barat telah dianalisis. Lima isolat yang menunjukkan aktivitas nitrogenase pada kondisi salin adalah Er B1 3, Er B1 4, Er B1 9, Er B2 10, dan Ptb B1 4. Gen nifH kelima sampel diidentifikasi menggunakan PCR menghasilkan amplikon berukuran ~360 bp. Aktivitas nitrogenase tertinggi berdasarkan Analisis Reduksi Asetilen (ARA) diperoleh pada isolat Er B2 10 yang memiliki kekerabatan terdekat dengan bakteri Providencia sp. Hasil yang diperoleh membuktikan bahwa beberapa bakteri asal sawah pesisir dapat menambat nitrogen pada kondisi salin.AbstractThe ability of nitrogen fixation by rhizosphere bacteria could be used to decrease salinity impact in coastal paddy field, due to nitrogenase capability, encoded by a nifH gene in component II. The objectives of this research are to analyze nitrogenase activity in saline condition and identify the presense of the nifH gene. A total of 50 isolates of the rhizosphere bacteria coastal from wetland areas of Eretan and Patimban, West Java, has been isolated and being analyzed. Among them, five isolates i.e. Er B1 3, ER B1 4, Er B1 9, Er B2 10 and Ptb B1 4, showed the nitrogenase activity under saline condition. The polymerase chain reaction (PCR) of the nifH gene from those five samples resulted in the amplicon size of  ~360 bp. The highest activity of nitrogenase assessed by acetylene reduction assay (ARA) was shown by Er B2 10 which closely related to bacteria of Providencia sp. The obtained result showed that several bacteria from coastal paddy field were able to conduct nitrogen fixation under saline stress.
Evaluasi Karakter Morfo-fisiologis Sumber Daya Genetik Padi Berumur Genjah Setyowati, Mamik; Hidayatun, Nurul; Sutoro, Sutoro; Kurniawan, Hakim
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 2, No 2 (2014)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2014.025.66-73

Abstract

Rice varieties with short maturity and high productivity can be achieved when there is available genetic resources in a sufficient level of variability. Morphological characters, such as leaf thickness and crop growth rate, could affect in crop productivity. Evaluation of seed germination rate, crop growth rate,  and their efficiency used for nitrogen have been done on 25 accessions of rice. Seed germination rate of B11283-6C-PN-5-MR-34-1-1-3, Lariang, B11742-RS-2-3-MR-34-1-2-3 reached 1,0-1,3 g/hill/day during seed initiation, while Dodokan and Silugonggo reached 0.7-0.8 g/hill/day. Danau Atas, B.11283-6C-PN-5-MR-2-3-Sl-1-2-1-1, B11742-RS-2-3-MR-34-1-2-3 and B11742-RS-2-3-MR-34-1-4-1 lines show good perfomance, moreover B11742-RS-2-3-MR-34-1-1-3, B11742-RS-2-3-MR-34-1-1-4 and B11742-RS-2-3-MR-34-1-1-4 lines produced grain yield higher than Silugonggo and Dodokan varieties. Those lines could be harvested at about 96 days after planting.