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All Journal e-Journal BUDIDAYA PERAIRAN JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX Jurnal Ilmiah Sains
Fitje Losung
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Environmental Science Mathematics Physics

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Journal : JURNAL ILMIAH PLATAX

 1 
The Antibacterial Activity Of Several Sponges From The Waters Of Tasik Ria Against Escherichia coli And Staphylococcus aureus bacteria Abrianto A. O. Rompis; Fitje Losung; Deiske A. Sumilat; Agung B. Windarto; Stenly Wullur; Laurentius T. X. Lalamentik
Jurnal Ilmiah PLATAX Vol. 7 No. 1 (2019): ISSUE JANUARY-JUNE 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.1.2019.21435

Abstract

The sponge is one of the sea organisms that has a prospect as a source of natural compounds including peptides, steroids, asetogenin, terpenoids, alkaloids, cyclic halide and nitrogen. This research was directed to obtain several species of sponges from the waters of Tasik Ria as well as testing the antibacterial activity of extracts from some of the sponge against the bacteria Escherichia coli and Staphylococcus aureus. From the identification, seven species of sponges were found, which consists of: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Cribochalina sp., Hyrtios sp., and Lendenfeldia sp. The tests of antibacterial activity of the extracts from these sponges against test bacteria E. coli and S. aureus showed some positive results. Extract from Axinosa sp. sponge(16 mm) showed the strongest antibacterial activity on Escherichia coli bacteria. Followed by Hyrtios sp. extract (13.5 mm), Aaptos sp. extract (13 mm), Lendenfeldia sp. extract (13 mm) and Cribochalinai sp. extract(10.5 mm). While the the tests on Staphylococcus aureus bacteria showed that the strongest antibacterial activity was found from Axinosa sp. sponge extract (16.5 mm), followed by the extract from Aaptos sp. (15 mm), Lendenfeldia sp. extract (14.5 mm), Hyrtios sp. extract(13.5 mm) and Cribochalina sp. extract (11 mm).Keywords: Sponge, antibacterial, Escherichia coli, Staphylococcus aureus ABSTRAK Spons merupakan salah satu biota laut yang sangat prospektif sebagai sumber senyawa bahan-bahan alami antara lain peptide, terpenoid, steroid, asetogenin, alkaloid, halide siklik dan senyawa nitrogen. Penelitian ini diarahkan untuk mendapatkan beberapa spesies spons dari perairan Tasik Ria serta menguji aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri Escherichia coli dan bakteri Staphylococcus aureus. Hasil identifikasi spons ditemukan sebanyak tujuh spesies yang terdiri dari: Amphimedon sp., Axinosa sp., Aaptos sp., Theonella sp., Hyrtios sp., Cribochalina sp. dan Lendenfeldia sp.. Aktivitas antibakteri dari beberapa ekstrak spons terhadap bakteri uji E. coli dan S. aureus terdapat diameter zona hambat bervariasi yaitu bakteri Escherichia coli menunjukkan aktivitas antibakteri ekstrak spons terkuat pada spons Axinosa sp (16 mm), disusul ekstrak spons Hyrtios sp. (13,5 mm), ekstrak spons Aaptos sp. (13 mm), ekstrak spons Lendenfeldia sp. (13 mm) dan ekstrak spons Cribochalinai sp. (10,5 mm).  Sedangkan pada bakteri Staphylococcus aureus menunjukkan aktivitas antibakteri ekstrak spons terkuat yaitu:  ekstrak spons Axinosa sp. (16,5 mm), disusul ekstrak spons Aaptos sp. (15 mm), ekstrak spons Lendenfeldia sp. (14,5 mm), ekstrak spons Hyrtios sp. (13,5 mm) dan ekstrak spons Cribochalina sp.(11mm).Kata Kunci : Spons, Antibakteri, Escherichia coli, Staphylococcus aureus
Test of Larvacide Activity from Some Sponge Extracts to Aedes aegypti Larvae Efra D. L. Wantah; R. E. P. Mangindaan; Fitje Losung
Jurnal Ilmiah PLATAX Vol. 6 No. 2 (2018): ISSUE JULY-DECEMBER 2018
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.6.2.2018.20637

Abstract

Marine organisms have been known produce certain compounds those could lead for medicine purposes. Sponges are one and the most studied for this aim. oneof the important biological activities which expected from sponge are larvacide activity. The aims this research was to test the larvacide activity aagainst Aedes aegypti larvae from some of sponge extract. Sponge samples were taken from Malalayang Waters, (N 01 ° 27'37 "E 124 ° 47'30") on November 2014 with the depth varies from 2-15 m  with SCUBA. The extraction, preparation of the larvae and activity testing was performed on Biomolecular and Marine Pharmacy Laboratory Faculty Fisheries and Marine Science. The sponge samples were cutted and soaked in 95% Ethanol for over night with 3 repetitions to obtain ethanolic extracts. The extract were filtered and evaporated using freeze dryer then tested onto 10 instars 3 instars m larvae that had been previously maintained. the test was made in triplowith 24 hours observation. abate was used as positive control while negative control clean water was used.The test results showed that of 11 Sponge tested, 10 species showed larvacidal activity and marine sponge extract Tedania sp. has the highest activity compared to 9 extracts. As a suggestion of this research the further purification of Tedania sp. extract is needed to know the structure of active compound.Keywords: Aedes aegypti, Larvacide, Sponge extractABSTRAKOrganisme laut yang dapat dikembangkan menjadi bahan sediaan obat antara lain sponge, dan merupakan salah satu organisme laut yang banyak diteliti. Beberapa aktivitas biologis penting yang diharapkan dari ekstrak sponge salah satunya adalah aktivitas larvasida. Penelitian ini bertujuan untuk menguji aktivitas larvasida nyamuk Aedes aegypti dari beberapa ekstrak Sponge. Sampel sponge diambil di perairan Malalayang, tepatnya di koordinat N 01°27’37” E 124°47’30” pada bulan November 2014 di kedalaman 2-15 m. Sedangkan untuk tahap ekstraksi, penyiapan larva uji dan pengujian aktivitas larvasida di lakukan di laboratorium Biomolekular dan Farmasitika Laut program studi Ilmu Kelautan, FPIK UNSRAT. Dalam penelitian yang dilakukan, sampel diambil di perairan menggunakan peralatan SCUBA. Setelah itu diekstrak dengan larutan etanol 95% dan direndam selama 24 jam dan dilakukan 3 kali pengulagan untuk mendapatkan ekstrak etanolik. Sampel dikeringkan dengan menggunakan alat freeze dryer kemudian diujikan ke 10 ekor larva nyamuk fase instar 3 yang telah dipelihara sebelumnya. Pengujian dilakukan sebanyak 3 kali pengulangan dengan lama pengamatan 24 jam pengamatan. Sebagai kontrol positif digunakan bubuk abate yang biasa dijual di pasaran sedangkan kontrol negative atau tanpa perlakuan digunakan air bersih. Data hasil pengamatan diolah menggunakan Microsoft excel.Hasil pengujian menunjukkan bahwa Dari 11 Sponge yang diuji, 10 jenis menunjukan aktivitas larvasida dengan persentase mortalitas yang bervariasi dan ekstrak sponge laut Tedania sp. memiliki aktivitas tertinggi dibandingkan dengan 10 jenis ekstrak sponge lain dalam pengujian. Sebagai saran dalam penelitian ini yaitu Perlu dilakukan pemurnian lanjut ekstrak lebih lanjut dari ekstrak sponge Tedania sp. yaitu ke tahap partisi dan Perlu adanya variasi konsentrasi dalam pengujian.Kata Kunci : Aedes aegypti, Larvasida, ekstrak Spong
Screening of the Proteolytic Bacteria Symbiont with Algae Gracillaria sp. Riorifki Kabense; Elvy L. Ginting; Stenly Wullur; Nickson J. Kawung; Fitje Losung; Jhon L. Tombokan
Jurnal Ilmiah PLATAX Vol. 7 No. 2 (2019): ISSUE JULY - DECEMBER 2019
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.7.2.2019.24487

Abstract

Marine algae are abundant natural resources in Indonesia but have not been optimally utilized. Utilization of seaweed is still limited as food ingredients such as Gracillaria sp. cultivated as an industrial export material. Algae Gracillaria sp. his life is symbiotic with a variety of types of bacteria. The aim of the study was to isolate and screening the protease activity of the symbionic bacteria of Gracillaria sp. This study succeeded in isolating 4 different bacteria based on morphological characteristics. The four isolates were S.G.,1,  S.G.2, S.G.3 and S.G.,4. Isolate S.G. 1 had the ability to produce ptotease with a proteolytic index of 1.5.Keywords : Gracilaria sp., Protease, Symbiont Bacteria ABSTRAKAlga laut merupakan sumberdaya alam yang melimpah di Indonesia tetapi belum optimal dimanfaatkan oleh masyarakat. Pemanfaatan rumput laut masih terbatas sebagai bahan makanan seperti Gracillaria sp. dibudidayakan sebagai bahan ekspor industri karajinan. Alga Gracillaria sp. hidupnya bersimbion dengan beraneka ragam jenis bakteri. Tujuan penelitian adalah untuk mengisolasi dan menguji aktivitas protease bakteri simbion alga Gracillaria sp. Penelitian ini berhasil mengisolasi 4 bakteri yang berbeda berdasarkan karakteristik morfologi. Keempat bekteri tersebut adalah S.G., 1 S.G., 2 S.G., 3 dan S.G., 4 Isolat bakteri  S.G., 1 memiliki kemampuan   menghasilkan ptotease dengan Indeks proteolitik sebesar 1,5.Kata Kunci  : Bakteri simbion, Gracillaria sp. protease
Co-Authors Abrianto A. O. Rompis Adnan Wantasen Agung B. Windarto Aylifia S. Somba Deiske A. Sumilat Deiske Sumilat Delpris Piter Diane J. Kusen Efra D. L. Wantah Elvy L. Ginting Esther D Angkouw Hariyani Sambali Henky Manoppo Jeksen Liem Jhon L. Tombokan Kreckhoff, Reni L. Laurentius T. X. Lalamentik Nickson J. Kawung R. E. P. Mangindaan Reiny A. Tumbol Remmy EP Mangindaan Remus B Maradou Remy E.P Mangindaan Riorifki Kabense Robert Bara Rosita AJ Lintang Stenly Wullur Veibe Warouw Wilmy E Pelle