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All Journal Marine Research in Indonesia JURNAL MASYARAKAT NEGERI ROKANIA Agricultural Science Makara Journal of Science
Arya Widyawan, Arya
Plant Protection Department, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia
Agriculture, Biological Sciences & Forestry Computer Science & IT Earth & Planetary Sciences Environmental Science Languange, Linguistic, Communication & Media Social Sciences Other

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POTENTIAL TOXIC CYANOBACTERIA IN THREE LAKES OF JAKARTA-BOGOR AREA, INDONESIA Prihantini, Nining Betawati; Widyawan, Arya; Rianto, Ronny; Ariyani, Yuni; Wardhana, Wishnu; Hendrayanti, Dian
Marine Research in Indonesia Vol 33 No 1 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5336.879 KB) | DOI: 10.14203/mri.v33i2.460

Abstract

The research was aimed to understand the composition and dominancy of aquatic microalgae, and to know the potential toxicity of Cyanobacteria occuring in three lakes of Jakarta-Bogor area. The study was done in 2006. The sampling sites were Lake Sunter 2 (North Jakarta), Lake Babakan (South Jakarta), and Lake Lido (Bogor). The water samples were taken using plankton-net (20 tan mesh) with horizontal tow. The results showed that. the number of microalgal genera in Sunter 2, Babakan, and Lido were different, i.e. 10 genera, 40 genera, and 14 genera, respectively. Cyanobacteria were dominant in Sunter 2 (96%) and Babakan (90.01%). The dominant Cyanobacteria in Sunter 2 was Planktothrix agardhii (53.48%), whereas in Babakan was Chroococcus dispersus (68.52%). The second abundant Cyanobacteria in Sunter 2 was Arthrospira (42.54%). Planktothrix agardhii and Arthrospira are known to produce harmful toxin.
BIODIVERSITAS CYANOBACTERIA DARI BEBERAPA SITU/DANAU DI KAWASAN JAKARTA-DEPOK-BOGOR, INDONESIA Prihantini, Nining Betawati; Wardhana, Wardhana; Hendrayanti, Dian; Widyawan, Arya; Ariyani, Yuni; Rianto, Ronny
Makara Journal of Science Vol. 12, No. 1
Publisher : UI Scholars Hub

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Abstract

Biodiversity of Cyanobacteria from several ponds/lakes of Jakarta-Depok-Bogor, Indonesia. Research about the exploration on Cyanobacteria diversity from several ponds/lakes of Jakarta-Depok-Bogor area was done. Five research locations were Sunter 2 Lake of North Jakarta, Babakan Pond of South Jakarta, Ulin-Salam dan Agathis Pond at UI Campus of Depok, dan Lido Lake of Bogor. Samples were taken by purposive sampling, and samples analysis using subsample methode. The sampling times were on August to September 2006. Three types of samples which were taken were planktonic samples, epifitic samples, and waters bank soil samples. The results showed that several toxic potential Cyanobacteria were found in several waters, even they were dominant at Sunter 2 Lake dan Agathis Pond (UI Campuss). Based on similarity levels of Cyanobacteria, the five of waters could be classied into 3 group, i.e. Sunter 2 Lake and Agathis Pond, Babakan dan Ulin-Salam Pond, dan Lido Lake. Lido Lake waters was very unique on distribution and diversity of Cyanobacteria. Based on diversity Index (H’) of Cyanobacteria, several waters condition could be catagories as moderate waters, i.e. Babakan, Situ Ulin-Salam, and Situ Agathis Pond. The epifitic and waters bank soil Cyanobacteria which were found were also found as planktonik species except Oscillatoria sp. 3. At least 26 isolate of Cyanobacteria were acquired such as Miicrocystis, Oscillatoria, Gloeocapsa, Merismopedia, Planktothrix, dan Arthrospira.
Cross amplification of 16S rRNA bacterial primer 27F/1492R on horticultural crop chloroplast genome Santosa, Adyatma Irawan; Hilmany, Taufiq; Dewi, Nurul Aulia; Rahmawati, Novianti Ekaputri; Putri, Erika Ananda; Hafidsya, Tiara; Setyaningrum, Ary Virgianti; Dewi, Rulia Ervina; Sari, Gracela Nanda Puspita; Nubatonis, Mars Buwn Fraset Manao; Widyawan, Arya
Agricultural Science Vol. 7 No. 2 (2024): March
Publisher : Faculty of Agriculture, Merdeka University Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55173/agriscience.v7i2.132

Abstract

Molecular techniques have been applied as fast and accurate detection methods of plant pathogenic bacteria. Bacteria-specific primer 27F paired with the universal primer 1492R was used in PCR to detect plant pathogenic bacteria in symptomatic leaves of tomato (Solanum lycopersicum L.), chili pepper (Capsicum annum L.), napa cabbage (Brassica rapa subsp. pekinensis L.), cabbage (Brassica oleracea var. capitata L.), and longan (Dimocarpus longan Lour.). The targeted single bands with length of 1400 bp were obtained but bidirectional sequencing of the PCR products recovered partial sequence of chloroplast instead of bacterial genomes. Thus, this result confirmed cross amplification of 27F/1492R primer pair against chloroplast genome of plants. Phylogenetic tree was constructed to separate the tested isolates according to their taxonomic relations: Order Sapindales, Solanales, and Brassicales. In-silico analysis on the new five and nineteen selected sequences in NCBI GenBank detected at least seven conserved regions with some single nucleotide polymorphisms. This report might be a cautionary advice for other researchers to avoid false positive results which could lead to misdiagnosis of bacterial plant diseases.
Sosialisasi Pemanfaatan Pupuk Organik Cair (POC) Urine Sapi Untuk Tanaman Buah Di Lingkungan Sekolah SMK Negeri 2 Rambah Wirawan, Purnama; Tua, Maruli; Juliarni; Yudistiro, Tito; Widyawan, Arya
JURNAL MASYARAKAT NEGERI ROKANIA Vol 5 No 2 (2024): JURNAL MASYARAKAT NEGERI ROKANIA
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Rokania

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56313/jmnr.v5i2.371

Abstract

Tujuan kegiatan pengabdian masyarakat adalah mengedukasi siswa SMK Negeri 2 Rambah tentang manfaat pupuk organik cair dari urin sapi pada tanaman buah. Metode yang digunakan meliputi pemberian materi dan demonstrasi. Pemberian materi bertujuan untuk meningkatkan kesuburan tanah serta pertumbuhan tanaman. Penggunaan pupuk organik cair dari urine sapi tidak menimbulkan efek samping karena bahan alami yang dibutuhkan tanaman. Akan tetapi, perlu memperhatikan dosis dan aturan penggunaan agar tidak merusak struktur tanah dan tanaman.
Pemberdayaan Masyarakat Melalui Pelatihan Pembuatan Virgin Coconut Oil (VCO) Bagi Ibu-ibu PKK Pangkalan Kerinci Barat JULIARNI; Tua, Maruli; Wirawan, Purnama; Widyawan, Arya; Yudistiro, Tito
JURNAL MASYARAKAT NEGERI ROKANIA Vol 6 No 1 (2025): JURNAL MASYARAKAT NEGERI ROKANIA
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Universitas Rokania

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56313/jmnr.v6i1.406

Abstract

Pengabdian masyarakat ini bertujuan untuk memberikan pelatihan kepada ibu-ibu PKK Pangkalan Kerinci Barat mengenai pemanfaatan dan pembuatan produk Virgin Coconut Oil (VCO). Metode kegiatan ini dilakukan dengan workshop. Dalam melakukan pemberdayaan masyarakat ini dilaksanakan tiga tahap yaitu tahap pesiapan, tahap pelaksanan dan evaluasi. Melalui kegiatan ini diharapkan dapat memberikan banyak manfaat bagi ibu-ibu PKK dalam membentuk usaha pembuatan pruduk Virgin Coconut Oil (VCO) sehingga diharapkan dapat membantu meningkatkan perekonomian masyarakat.
Co-Authors Achmad Noerkhaerin Putra Adyatma Irawan Santosa Ariyani, Yuni Dewi, Nurul Aulia Dewi, Rulia Ervina Dian Hendrayanti Hafidsya, Tiara Hilmany, Taufiq Juliarni Nubatonis, Mars Buwn Fraset Manao Prihantini, Nining Betawati Purnama Wirawan Putri, Erika Ananda Rahmawati, Novianti Ekaputri Rianto, Ronny Sari, Gracela Nanda Puspita Setyaningrum, Ary Virgianti Tua, Maruli Wardhana wardhana Wardhana, Wishnu Yudistiro, Tito