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POTENTIAL TOXIC CYANOBACTERIA IN THREE LAKES OF JAKARTA-BOGOR AREA, INDONESIA Prihantini, Nining Betawati; Widyawan, Arya; Rianto, Ronny; Ariyani, Yuni; Wardhana, Wishnu; Hendrayanti, Dian
Marine Research in Indonesia Vol 33, No 1 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5336.879 KB) | DOI: 10.14203/mri.v33i2.460

Abstract

The research was aimed to understand the composition and dominancy of aquatic microalgae, and to know the potential toxicity of Cyanobacteria occuring in three lakes of Jakarta-Bogor area. The study was done in 2006. The sampling sites were Lake Sunter 2 (North Jakarta), Lake Babakan (South Jakarta), and Lake Lido (Bogor). The water samples were taken using plankton-net (20 tan mesh) with horizontal tow. The results showed that. the number of microalgal genera in Sunter 2, Babakan, and Lido were different, i.e. 10 genera, 40 genera, and 14 genera, respectively. Cyanobacteria were dominant in Sunter 2 (96%) and Babakan (90.01%). The dominant Cyanobacteria in Sunter 2 was Planktothrix agardhii (53.48%), whereas in Babakan was Chroococcus dispersus (68.52%). The second abundant Cyanobacteria in Sunter 2 was Arthrospira (42.54%). Planktothrix agardhii and Arthrospira are known to produce harmful toxin.
Application of N2-Fixing Cyanobacteria Nostoc sp. SO-A31 to Hydroponically Grown Water Spinach (Ipomoea aquatic L.) Salamah, Andi; Fadilah, Nurrahmi; Khoiriyah, Istatik; Hendrayanti, Dian
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.1867

Abstract

For this research, an application of cyanobacteria Nostoc sp. SO-A31 as a nitrogen source for the growth of water spinach (Ipomoea aquatica L.) was carried out using a modified Deep-Water Culture (DWC) hydroponic system, outdoors. A Hoagland medium was used for the growth medium, with the absence or presence of ammonium and nitrate as the nitrogen sources. A 0.7 g fresh weight biomass of 21-day-old Nostoc sp. SO-A31 was inoculated into the system. The four treatment media for this study were HA0 (Hoagland, ammonium free+inoculant), HN0 (Hoagland, nitrate free+inoculant), HA0N0 (Hoagland, ammonium free and nitrate free+inoculant), and HI (Hoagland with ammonium and nitrate +inoculant). AB-mix and complete Hoagland media were used as controls. The result showed that water spinach cultured on HA0 had good vegetative growth, as shown by the high yield of biomass, high number of leaves, high stem growth, and long roots. Inoculation of Nostoc sp. SO-A31 elongated the root of the water spinach plants in all treatments. The presence of Nostoc sp. SO-A31 in the complete Hoagland medium, though, caused chlorosis of the water spinach leaves. This study suggests that water spinach is a nitrate-dependent leafy vegetable.
Vegetative Cell Division and Nuclear Translocation in Three Algae Species of Netrium (Zygnematales, Chlorophyta) DIAN HENDRAYANTI
HAYATI Journal of Biosciences Vol. 13 No. 1 (2006): March 2006
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (71.731 KB) | DOI: 10.4308/hjb.13.1.39

Abstract

Three species of Netrium oblongum, N. digitus v. latum, and N. interruptum were studied for their mode in the vegetative cell division and nuclear translocation during mitosis using light and fluorescence microscopy. The process of cell division in the three species began with the prominent constriction at the chloroplast in both semicells about half way from the apex. The constriction of chloroplast was mostly visible in N. digitus v. latum. Soon after nucleus divided, septum was formed across the cell and cytokinesis occurred. Observation with fluorescence microscope showed that the movement of nucleus moved back into the center of daughter cells was not always synchronous. Division of chloroplast in N. oblongum and N. digitus v. latum were different with that of N. interruptum. Chloroplast division in two former species occured following the movement of the nucleus down semicell. However, in N. interruptum, chloroplast divided later after nucleus occupied the position at the center of the daughter cells. Cell restoration started after the completion of mitosis and cytokinesis. Key words: Cell division, conjugating alga, mitosis, Netrium
Application of Biological Nitrogen Fixation Cyanobacteria To Paddy Plant Cultivated Under Deep-Water Culture System Dian Hendrayanti; Iman Rusmana; Dwi Andreas Santosa; Hamim Hamim
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.8510

Abstract

 The Biological Nitrogen Fixing (BNF) cyanobacteria can reduce atmospheric nitrogen into ammonium. This ability makes BNF cyanobacteria a potential eco-friendly N-source for soil-planted pad-dy. Apart from a few success stories of BNF cyanobacteria applica-tion in the rice field, its role as an ammonium producer is still an open question. There is also a possibility that indeed cyanobacteria biomass which provides nitrogen through the biological decomposing process. This study aimed to analyze the influence of three strains BNF cyanobacteria on paddy grown in the Deep-Water Culture (DWC) hydroponic system. Yoshida Nutrient Solution was used as a growth medium with the application of 0, 45.7, and 91.4 gL-1 of ammonium nitrate (NH4NO3) with five replications. The result showed that inter-action between cyanobacteria and nitrogen significantly influenced the total nitrogen of paddy plant, but not the paddy plant height, the num-ber of tillers, and biomass. Cyanobacterial filaments were found most-ly attached to the paddy root rather than floated in the DWC system. Paddy treated with cyanobacteria had more lateral root than control. The result suggested that cyanobacteria support paddy growth indi-rectly through nitrogen deposition in plant tissue and root development. 
Biodiversity, Roles, and Potency of Bacteria in Agricultural Land Wahyu Purbalisa; Dian Hendrayanti; Wahida Annisa Yusuf
Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan Vol 19, No 3 (2022): November 2022
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1170.137 KB) | DOI: 10.14710/presipitasi.v19i3.520-531

Abstract

Biotic and abiotic factors influence biodiversity. Environment and human activities change biodiversity. Human activities to get their food through agricultural activities affect the diversity of bacteria in agricultural lands. Cultivation techniques, plant species, plant growth stadia, and soil influence the diversities of bacteria in agricultural land. The dominant bacteria on agricultural land are from the phyla Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, and Firmicutes. Proteobacteria play a role in the nitrogen, carbon, and sulphur cycles, Actinobacteria in the carbon cycle, and Acidobacteria in the nitrogen cycle. Bacteroidetes work as decomposers of organic matter. Firmicutes are required for biocontrol and plant growth. The high use of pesticides in agricultural land has decreased the diversity of bacteria. Indigenous bacteria that survive in the condition of high pesticide residue contamination have the potential as remediation agents for pesticide residues. Indigenous bacteria can degrade pesticide residues through oxidation-reduction and complexation reactions, thereby reducing the level of pesticide contamination.
Phylogeny of Indonesian Nostoc (Cyanobacteria) Isolated from Paddy Fields as Inferred from Partial Sequence of 16S rRNA Gene Hendrayanti, Dian; Kusmadji, Lestari Rahayu; Yuliana, Pratiwi; Amanina, Mardhotillah Asma
Makara Journal of Science Vol. 16, No. 3
Publisher : UI Scholars Hub

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Abstract

In order to collect Indonesian Nostoc, isolation of soil microflora from several paddy fields in West Java, Bali, and South Celebes was carried out. Fast-growing isolates of Nostoc were selected to describe and perform molecular identification using partial sequences of 16S rRNA. The results showed that partial sequences of 16S rRNA could not resolve the phylogeny of the isolates. However, it supported the morphological studies that recognize isolates as different species of Nostoc. Potential use of Nostoc as a nitrogen source for paddy growth was carried out using six strains as single inoculums. A total biomass of 2 g (fresh weight) for each strain was inoculated, respectively, into the pot planted with three paddy plants. This experiment was conducted in the green house for 115 days. Statistical analyses (ANOVA; α = 0.05) showed that of six strains tested in this study, only strain GIA13a had influence on the augmentation of root length and the total number of filled grains.
BIODIVERSITAS CYANOBACTERIA DARI BEBERAPA SITU/DANAU DI KAWASAN JAKARTA-DEPOK-BOGOR, INDONESIA Prihantini, Nining Betawati; Wardhana, Wardhana; Hendrayanti, Dian; Widyawan, Arya; Ariyani, Yuni; Rianto, Ronny
Makara Journal of Science Vol. 12, No. 1
Publisher : UI Scholars Hub

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Abstract

Biodiversity of Cyanobacteria from several ponds/lakes of Jakarta-Depok-Bogor, Indonesia. Research about the exploration on Cyanobacteria diversity from several ponds/lakes of Jakarta-Depok-Bogor area was done. Five research locations were Sunter 2 Lake of North Jakarta, Babakan Pond of South Jakarta, Ulin-Salam dan Agathis Pond at UI Campus of Depok, dan Lido Lake of Bogor. Samples were taken by purposive sampling, and samples analysis using subsample methode. The sampling times were on August to September 2006. Three types of samples which were taken were planktonic samples, epifitic samples, and waters bank soil samples. The results showed that several toxic potential Cyanobacteria were found in several waters, even they were dominant at Sunter 2 Lake dan Agathis Pond (UI Campuss). Based on similarity levels of Cyanobacteria, the five of waters could be classied into 3 group, i.e. Sunter 2 Lake and Agathis Pond, Babakan dan Ulin-Salam Pond, dan Lido Lake. Lido Lake waters was very unique on distribution and diversity of Cyanobacteria. Based on diversity Index (H’) of Cyanobacteria, several waters condition could be catagories as moderate waters, i.e. Babakan, Situ Ulin-Salam, and Situ Agathis Pond. The epifitic and waters bank soil Cyanobacteria which were found were also found as planktonik species except Oscillatoria sp. 3. At least 26 isolate of Cyanobacteria were acquired such as Miicrocystis, Oscillatoria, Gloeocapsa, Merismopedia, Planktothrix, dan Arthrospira.
Eksplorasi Biodiversitas Mikroalga Epifit di Perairan Teluk Hurun, Lampung, Menggunakan Analisa Morfologi dan Metode Metabarkoding Widiarti, Riani; Hendrayanti, Dian; Humaida, Milka; Sani, Lalu M. Iqbal
Jurnal Kelautan Nasional Vol 19, No 2 (2024): Agustus
Publisher : Pusat Riset Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jkn.v19i2.14509

Abstract

Penelitian mengenai kelimpahan mikroalga epifit di perairan Teluk Hurun belum pernah dilakukan, padahal beberapa mikroalga epifit berpotensi menimbulkan Harmful Algal Blooms. Tujuan dari penelitian ini adalah untuk mengidentifikasi dan menganalisis kelimpahan mikroalga epifit pada makroalga Padina di perairan Teluk Hurun, terutama untuk spesies yang berpotensi menyebabkan HAB sehingga memungkinkan dilakukannya upaya mitigasi. Makroalga Padina diambil secara acak dari kawasan rataan terumbu, dimasukkan ke dalam botol sampel, dan dikocok kuat selama 1 menit. Sampel air disaring menggunakan saringan bertingkat, untuk kemudian diawetkan dan diamati di bawah mikroskop. Analisis eDNA juga dilakukan dengan menyaring sampel air tersebut melalui kertas millipore berukuran pori 0,45 µm menggunakan filtering set, kemudian diamplifikasi dengan set primer 18s v9, dan dilakukan pengurutan basa DNA melalui Illumina iSeq. Hasil morfogenetik menunjukkan bahwa ditemukan mikroalga dari kelompok Bacillariophycea dengan kelimpahan dan proporsi keberadaan tertinggi yaitu Nitzschia dan Navicula. Meskipun kelompok Dinophycea tidak ditemukan pada pengamatan morfologi, namun analisis eDNA menunjukkan 2 spesies yang berpotensi menyebabkan HAB, yaitu Gambierdiscus sp. dan Prorocentrum mexicanum. Sel-sel dari kelompok Dinophyceae mungkin mengalami kerusakan selama proses perlakuan atau pengawetan, namun materi genetik yang terlarut dalam sampel air dapat terdeteksi menggunakan analisis eDNA.
Characteristics and Potential Indigenous Bacteria Isolated from Shallot Farming in Brebes Regency as Diazinon Pesticide Remediation Agents Purbalisa, Wahyu; Hendrayanti, Dian; Yusuf, Wahida Annisa
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

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Abstract

Diazinon is an organophosphate pesticide that is widely used by shallot farmers in Brebes Regency. Due to its toxicity, diazinon residue in agricultural lands as well as plants’ cultivation is harmful to humans and the environment. Using indigenous soil bacteria as a remediation agent is an alternative solution to overcome diazinon contamination. Firstly, this study aimed to characterize isolates from diazinon-contaminated shallot farming. The second objective was to test the diazinon tolerance of the isolates in various diazinon concentrations. Isolation was carried out using nutrient agar (NA) media containing diazinon concentrations of 5, 25, 50, and 100 ppm. The characterization of isolates was based on morphology and physical chemistry, including gram staining, catalase-oxidase, and carbohydrate fermentation tests. A hypersensitive test was conducted to ensure the non-pathogenic properties of the isolates. Diazinon degradation was tested using gas chromatography. This study successfully obtained 10 isolates, six of which were Gram-positive bacteria. The colonial shapes were various, including circular, filamentous, and irregular. All isolates were neutrophilic bacteria, positively reacted to catalase tests, and could use glucose in carbohydrate fermentation tests. The hypersensitive test demonstrated that all isolates were non-pathogenic. All isolates adapted to various concentrations of diazinon up to 50 ppm. The highest diazinon degradation (82.2%) was performed by Gram positive-filamentous strain KD1-D100.1. The application of indigenous isolates in this research will be studied further by testing the single and consortium inoculation for soil remediation.
Mengeksplorasi Keanekaragaman Mikroalga di Indonesia: Memanfaatkan Potensi Bioremediasi Timbal Andriani, Feni; Hendrayanti, Dian; Yasman, Yasman
BIOEDUSCIENCE Vol 8 No 1 (2024): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/13225

Abstract

Background: Indonesia's biodiverse microalgae, numbering 2060 species, thrive in diverse environments, offering potential in pharmaceuticals, cosmetics, fuel, and heavy metal bioremediation. Lead pollution from mining poses risks, prompting stringent government contamination limits. Microalgae-based bioremediation, notably Chlamydomonas reinhardtii and Chlorella vulgaris removing up to 90% of lead, suggests further exploring Indonesia's microalgae diversity. Methods:  Data sourcing (2013-2023) employed Google and Google Scholar using specific keyword combinations across Indonesian provinces. Seventy-eight sources underwent analysis and visualization via Microsoft Excel 2021. Results: Microalgae's potential as lead bioremediation was explored across classes like Chlorophyceae, Cyanophyceae, and Bacillariophyceae. Chlorophyceae, exemplified by Chlorella and Scenedesmus, exhibit varied cell sizes and complex cell walls, aiding metal ion binding. Cyanophyceae like Cyanospira capsulata and N. commune synthesize diverse EPS compositions, indicating their potential in lead remediation. Conclusions: Indonesia's microalgae diversity across habitats, especially in the Chlorophyceae and Cyanophyceae classes, suggests a significant role in lead bioremediation. Their adaptability and diverse compositions highlight their potential for sustainable practices in combatting environmental lead contamination.