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Optimization of Bioencapsulation of Bacillus sp. to Increase Resistance to Bacterial Wilt and Growth of Cayenne Pepper Ajeng Distya Anjani; Yenny Wuryandari; Safira Rizka Lestari
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 4 (2025): August 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v14i4.1325-1334

Abstract

Cayenne pepper has a high production demand. The high demand does not match the amount of production produced. One of the factors for decreased production is the presence of bacterial wilt disease caused by Ralstonia solanacearum. Bacterial wilt control can be done with the application of antagonistic bacteria, namely Bacillus sp. Bacillus sp. application requires an optimal formulation to maintain bacterial activity in the soil, one of which is Bacillus sp. encapsulation. This study aims to determine the effectiveness of Bacillus sp. encapsulation as a formulation of biological agent bacteria for the control of bacterial wilt disease and cayenne pepper plant growth. This study used the encapsulating material concentration and dosage factors for application to plants. Encapsulation was made using the extrusion method. The results showed that the concentration of 2% sodium alginate and 1.5% gelatin gave a significant difference in disease intensity of 18.89% and in the growth parameters of cayenne pepper plants were able to reach a height of 30.58 cm and a root length of 17.18 cm. The right concentration and high dose provide the ability to suppress intensity and encourage maximum growth
Effectiveness of Endophytic Bacteria Rhizophora sp. from Mangrove Plants to Control Root-Knot Nematodes (Meloidogyne spp.). Nurdiah Khasanah; Safira Rizka Lestari; Dita Megasari
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 3 (2025): June 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v14i3.1110-1117

Abstract

Meloidogyne spp. is a root-knot nematode that can cause damage to plants up to 68%. The common control is the use of pesticides, but in the long term, it can damage the soil, so it is necessary to use biological agents that can control Meloidogyne spp. without causing environmental damage. The purpose of this study was to determine the ability of endophytic bacteria to control root-knot nematodes Meloidogyne spp.. The method used in this study was the exploration of endophytic bacteria from the Wonorejo mangrove ecotourism area, Surabaya using purposive sampling method. The five best endophytic bacteria were tested further with antagonistic tests. This test was carried out in vitro using 3 concentrations, namely 106, 108, and 1010 CFU/ml with 3 repetitions. The obtained results were tested using probit analysis to obtain the LC50 values. It was found that LC50 values for the most effective concentration to kill 50% of the Meloidogyne sp population is 109 and 1010 CFU/ml. It was also revealed that the isolate that is most capable of controlling Meloidogyne spp. nematodes is isolate A43. Keywords: Antagonist test, Endhopic bacteria, LC50, Mangrove, Meloidogyne spp.