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Carrier Materials Differences on Bioencapsulation Formulation Affect Bacillus sp. Isolates Yenny Wuryandari; Penta Suryaminarsih; Safira Rizka Lestari
Nusantara Science and Technology Proceedings 8th International Seminar of Research Month 2023
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2024.4162

Biological agent Bacillus sp. Based on the results of previous research, direct use of isolated microorganisms BCZ14, BCZ16, and BCZ20 was able to suppress Fusarium wilt disease. However, Bacillus sp. formulations in suspension form are less effective when applied in the field. This research focuses on analyzing the viability of three isolates of Bacillus sp. against several combinations of bioencapsulation coating materials. The experimental design used was a completely randomized design with a combination of treatment of three isolates and three types of coating materials, namely sodium alginate (B1), carrageenan (B2), and chitosan (B3) each 2%. Each treatment combination was repeated five times. Based on the experiments that have been carried out, the bioencapsulation formulation using 2% chitosan coating material was able to maintain the viability of the three isolates of Bacillus sp. BCZ14, BCZ16, and BCZ20. The population density of the three isolates of Bacillus sp. persisted consistently from the first week to the last week of observation. Therefore, the 2% chitosan coating material can be said to be able to maintain the viability of Bacillus sp. within six weeks.

Natural Ingredients of Beans as Growth Medium and Propagation of Endophytic Bacteria from Lowland Eggplant Arika Purnawati; Safira Rizka Lestari; Iis Purnamawati
Nusantara Science and Technology Proceedings 7st International Seminar of Research Month 2022
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2023.3308

Endophytic bacteria are defined as a group of microbes that live in plant tissues that do not cause disease and are mutually related to the host plant, can be isolated from all parts of the plant were roots, stems, leaves, and seeds. Endophytic bacteria have the potential to produce natural products for agriculture, increase plant growth, and support the sustainable development of agriculture. The growth of endophytic bacteria in vitro generally uses a medium that contains nutrients including macronutrients, namely elements (C, H, O, and N), micronutrients, namely non-metallic elements (S and P), metallic elements (Ca, Zn, Na, K, Cu). , Mn, Mg, and Fe), vitamins, water, and energy. To meet these growth requirements, currently the growth and propagation of endophytic bacteria generally still use synthetic media which are expensive and in the field, mainly by farmers, are not easy to obtain. The study aimed to obtain an alternative medium made from beans for the growth and propagation of endophytic bacteria from lowland eggplants. The types of beans were soybeans, green beans, red beans, cowpeas, peanuts, and Nutrient Agar (NA) as control. Inoculation into the medium was done using the spread plate technique. This technique is preceded by a dilution and at dilution of 10-7 was taken 0.1 ml then to be spread on the growing medium. Observations were growing and colony counting was done 24 hours after inoculation. The results were: (1) endophytic bacteria from lowland eggplant can grow well on all mediums with the best growth on NA medium, (2) all media can be used as the propagation of endophytic bacteria from lowland eggplant plants, (3) the assumed of the growth curve is logarithmic with 4 phases were lag phase, exponential phase, stationer phase, death phase.

Characterization of Bacterial Blight Pathogen on Rice Plants in Sidoarjo Sayekti, Ninik Ari; Purnawati, Arika; Lestari, Safira Rizka
Journal of Applied Plant Technology Vol 3 No 2 (2024): Journal of Applied Plant Technology (JAPT)
Publisher : Agrotechnology Study Program, Faculty of Agriculture, Wijaya Kusuma Surabaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/xwdype52

Rice (Oryza sativa L.) is a staple crop for all Indonesian people. Rice production in Indonesia in 2019 experienced a decline in production. Plant disease attacks are a factor that influences the decline in rice production. Bacterial leaf blight is an important disease in rice plants that can cause yield losses of up to 50%. Characterization of disease-causing bacteria needs to be carried out to develop appropriate, effective and efficient control in suppressing the development of bacterial leaf blight. The aim of this research was to characterize the morphological and physiological characteristics of the bacteria that cause bacterial leaf blight. The method used in this research included the isolation of bacteria that cause bacterial leaf blight disease carried out on rice plantations in Pulungan Village, Sedati, Sidoarjo. Identification is carried out through macroscopic and microscopic observations, analysis of physiological properties through the gram staining test, fermentative oxidative test, yellow colony test on YDC media, urease test, hypersensitivity test, and pathogenicity test. The results of the research show that the bacteria that cause bacterial leaf blight have the morphological characteristics of being yellow, round and shiny. The physiological characteristics of the bacterial isolate are gram-negative bacteria, are oxidative, yellow in YDC media, and react negatively in the urease test.

EFIKASI KONSORSIUM Bacillus sp. DAN Pseudomonas fluorescens TERHADAP LAYU FUSARIUM PADA TANAMAN CABAI RAWIT (Capsicum frutescens L.) Nensi Agustina; Arika Purnawati; Endang Triwahyu Prasetyawati; Safira Rizka Lestari
Jurnal Agrotek Tropika Vol 12, No 3 (2024): JURNAL AGROTEK TROPIKA VOL 12, AGUSTUS 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i3.5724

Fusarium oxysporum f.sp. capsici merupakan patogen penting pada cabai rawit karena menyebabkan penyakit layu, merugikan dan menurunkan produksi hingga 50%. Pengendalian secara kimiawi menggunakan fungisida telah banyak dilakukan namun berdampak negatif terhadap lingkungan. Berdasarkan percobaan di lapangan, aplikasi konsorsium bakteri Bacillus sp. dan Pseudomonas fluorescens dapat mengendalikan penyakit layu Fusarium. Tujuan dari penelitian ini adalah untuk mengetahui tingkat efikasi dan pengaruh aplikasi konsorsium Bacillus sp. dan Pseudomonas fluorescens terhadap penyakit layu yang disebabkan oleh F. oxysporum f.sp. capsici. Penelitian ini dilakukan dengan menginokulasi 100 ml F. oxysporum f.sp. capsici (104 spora ml-1) dan 25 ml konsorsium bakteri (109 CFU mL-1), lalu diamati tingkat efikasi dan pengaruhnya terhadap perkembangan penyakit layu Fusarium. Rancangan penelitian ini adalah Rancangan Acak Lengkap (RAL) yang terdiri dari dua faktor yaitu konsorsium Bacillus sp. (Ba9) dengan Pseudomonas fluorescens, konsorsium Bacillus sp. (Ba17) dengan Pseudomonas fluorescens, ulangan sebanyak tiga kali dan analisis data menggunakan uji Tukey's HSD. Hasil penelitian bahwa konsorsium bakteri dapat menurunkan intensitas penyakit rata-rata 45-66%.

Diagnosis and Etiology of Seedling Mortality in Cavendish Banana (Musa acuminata L.) at PT Great Giant Pineapple Nursery Nugraha, Rafi Dwi; Lestari, Safira Rizka
Journal of Applied Plant Technology Vol 4 No 1 (2025): Journal of Applied Plant Technology (JAPT)
Publisher : Agrotechnology Study Program, Faculty of Agriculture, Wijaya Kusuma Surabaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/tva7gb30

This study aimed to diagnose the causes of seedling mortality in Cavendish banana (Musa acuminata L.) at PT Great Giant Pineapple Nursery. Clinical symptoms observed included leaf wilting, root rot, and blackened corms, primarily under high-humidity conditions. Laboratory isolation and morphological identification confirmed four fungal pathogens as the primary etiological agents: Heart rot (Phytophthora sp.), Aspergillus rot (Aspergillus sp.), fruit rot (Rhizopus sp.), and damping-off (Pythium sp.). Phytophthora sp. was particularly aggressive, causing severe vascular necrosis. Environmental factors such as excessive moisture, poor drainage, and contaminated nursery substrates were found to exacerbate disease incidence. The findings underscore the need for improved nursery management, including strict sanitation, optimized irrigation, and preventive fungicide applications. Recommendations also include the use of disease-free planting materials and enhanced ventilation to reduce fungal proliferation. Implementing these measures can significantly decrease seedling mortality and improve the production of healthy Cavendish banana plants.

Pengaruh Konsentrasi Kalsium Klorida pada Viabilitas dan Daya Sweelling Beads Enkapsulasi Paenibacillus polymyxa secara In Vitro Sari, Zulfa Novita; Nirwanto, Hery; Lestari, Safira Rizka
BIOEDUSCIENCE Vol 9 No 2 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/16364

Background: Paenibacillus polymyxa is a potent antagonist that can be utilized as a biological agent. The use of biological agents has gained interest among farmers, but their application in liquid formulations has not consistently maintained the optimal stability and viability of microorganisms. One promising approach to overcome this problem is bioencapsulation, which is a method of wrapping biological agents to protect bacteria in the soil and increase their efficiency. This study aims to assess the encapsulation efficiency, measure the viability of microorganisms in the beads, and determine the difference in swelling power of beads made with various concentrations of calcium chloride (CaCl2) as a binding agent. Method: Beads were produced using the extrusion method by combining Paenibacillus polymyxa suspension, sodium alginate suspension, and adding CaCl2 at concentrations of 1%, 3%, and 5%. Results: The results showed that different CaCl2 concentrations can affect the viability of Paenibacillus polymyxa in beads. Beads made with CaCl2 at 3% concentration were the best results in the encapsulation efficiency test compared to beads made with 1% and 5% CaCl2 binders. In comparison, beads with 3% and 5% CaCl2 concentrations were able to maintain the viability of microorganisms at a higher level and for a longer time than beads using CaCl2 at 1% concentration. The decrease in viability and swelling power of the beads is thought to be caused by the carrier material used and the storage conditions. Conclusion: Bead treatment with 3% calcium chloride concentration was the best treatment for encapsulation efficiency in absorbing Paenibacillus polymyxa, amounting to 98.21%.

Utilization of Trichoderma sp. and compost to increase nitrogen in specific soil types as planting media Maroeto, Maroeto; Wijayanti, Fitri; Lestari, Safira Rizka; Maghfiroh, Ainur Risma Miftakhul
Jurnal Ilmu Pertanian Vol 10, No 2 (2025): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.96370

Nitrogen deficiency dramatically affects plant growth, development, and productivity. This study aims to determine the utilization of Trichoderma sp. and compost in increasing the nitrogen content of several types of soil used as planting media. The research was conducted using an experimental method arranged in a completely randomized design (CRD) factorial pattern. The first factor is the soil types, namely Alfisol, Inceptisol, and Vertisol. The second factor is dose of compost consisting of 4 levels, namely K1 (0 tons ha-1 or no compost), K2 (10 tons ha-1), K3 (20 tons ha-1), and K4 (30 tons ha-1). The third factor is the concentration of Trichoderma sp., which consists of 4 levels, namely T1 (0 CFU ml-1), T2 (106 CFU ml-1), T3 (108 CFU ml-1), and T4 (1010 CFU ml-1). Results showed that the combination of compost and Trichoderma sp. had a natural effect on increasing the N content of each soil type. N-total content from highest to lowest was found in soil types such as Inceptisol (0.384%), Alfisol (0.243%), and Vertisol (0.230%). Combining compost and Trichoderma sp. increases sustainable nitrogen availability and is more environmentally friendly.

Utilization of Trichoderma sp. and compost to increase nitrogen in specific soil types as planting media Maroeto, Maroeto; Wijayanti, Fitri; Lestari, Safira Rizka; Maghfiroh, Ainur Risma Miftakhul
Jurnal Ilmu Pertanian Vol 10, No 2 (2025): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.96370

Nitrogen deficiency dramatically affects plant growth, development, and productivity. This study aims to determine the utilization of Trichoderma sp. and compost in increasing the nitrogen content of several types of soil used as planting media. The research was conducted using an experimental method arranged in a completely randomized design (CRD) factorial pattern. The first factor is the soil types, namely Alfisol, Inceptisol, and Vertisol. The second factor is dose of compost consisting of 4 levels, namely K1 (0 tons ha-1 or no compost), K2 (10 tons ha-1), K3 (20 tons ha-1), and K4 (30 tons ha-1). The third factor is the concentration of Trichoderma sp., which consists of 4 levels, namely T1 (0 CFU ml-1), T2 (106 CFU ml-1), T3 (108 CFU ml-1), and T4 (1010 CFU ml-1). Results showed that the combination of compost and Trichoderma sp. had a natural effect on increasing the N content of each soil type. N-total content from highest to lowest was found in soil types such as Inceptisol (0.384%), Alfisol (0.243%), and Vertisol (0.230%). Combining compost and Trichoderma sp. increases sustainable nitrogen availability and is more environmentally friendly.

DAMPAK KOMBINASI JENIS TANAH, KOMPOS DAN Trichoderma sp. TERHADAP KERAPATAN SPORA Trichoderma sp. Subhan, Aprellia Sofiatul; Arifin, Moch.; Wijayanti, Fitri; Maroeto, Maroeto; Lestari, Safira Rizka
Jurnal Agrotek Tropika Vol. 13 No. 1 (2025): JURNAL AGROTEK TROPIKA VOL 13, FEBRUARI 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i1.8626

Trichoderma sp. merupakan jamur yang dapat berperan sebagai agen pengendali hayati karena dapat memarasit jamur lainnya dan mempercepat dekomposisi bahan organik. Penelitian ini bertujuan untuk melihat proses dekomposisi bahan organik pada berbagai jenis tanah dengan bantuan Trichoderma sp. sebagai dekomposer. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari tiga faktor. Faktor pertama yaitu macam jenis tanah J1: Alfisol; J2: Inceptisol; dan J3: Vertisol. Faktor kedua yaitu dosis kompos K0: 0 ton/ha (0 gr/4,4kg tanah); K1: 10 ton/ha (15 gr/4,4 kg tanah); K2: 20 ton/ha (30 gr/4,4 kg tanah); dan K3: 30 ton/ha (45 gr/4,4 kg tanah). Faktor ketiga yaitu taraf pemberian Trichoderma sp. T1: 15 ml dari 106 spora/ml; T2: 15 ml dari 108 spora/ml; dan T3: 15 ml dari 1010 spora/ml. Hasil penelitian yang didapat pada perlakuan Jenis tanah, Kompos dan Trichoderma sp. tidak memberikan interaksi yang nyata terhadap kerapatan spora Trichoderma sp. pada perlakuan jenis tanah dan dosis kompos memberikan pengaruh nyata terhadap kerapatan spora Trichoderma sp. Pada jenis tanah Inceptisol memberikan nilai tertinggi di semua interval pengamatan kerapatan spora Trichoderma sp. dengan dosis kompos 20 ton/ha dan 30 ton/ha pada 14 Hari Setelah Inkubasi (HSI), 30 ton/ha pada 28 Hari Setelah Inkubasi (HSI) hingga 42 Hari Setelah Inkubasi (HSI) dan 20 ton/ha pada 56 Hari Setelah Inkubasi (HSI).

EVALUATING THE LONG-TERM STORAGE TIME VIABILITY AND SIZE DYNAMICS OF BACILLUS SP. BIOENCAPSULATION IN SODIUM ALGINATE MATRIX Saputra, Mochammad Mirza; Wuryandari, Yenny; Rahmadhini, Noni; Lestari, Safira Rizka
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2549

The use of biological agents such as Bacillus sp. bacteria has begun to be widely used by farmers as a new form of control. Bacillus sp. needs to require special methods in its application because it has certain living conditions, and Bacillus sp. is vulnerable to environmental pressures. Bioencapsulated formulation in the form of granules is considered effective as a form of bacterial application in soil because it is able to protect Bacillus sp. and maintain survival. This study aims to determine the best concentration of sodium alginate in maintaining the viability of Bacillus sp. at the in vitro stage and to see changes in the size of the beads. The beads were made using extrusion method by combining Bacillus sp. suspension and sodium alginate suspension at 1%, 1.5%, and 2% concentration. The concentration of sodium alginate was not a major factor in changes in bead size and viability test results experienced significant changes in each observation. 1.5% and 2% concentrations can reach the highest viability value of 3x106 CFU/mL and decreased during 1 month of observation. Changes in bead size and viability were caused by the alginase enzyme produced by Bacillus sp.

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