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All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal Journal Of World Science
T. Maulana, T.
Research Centre for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46 Cibinong 16911
Agriculture, Biological Sciences & Forestry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Energy Engineering Health Professions Law, Crime, Criminology & Criminal Justice Social Sciences

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Journal : Tropical Animal Science Journal

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Proteomic Analysis of Pesisir Bull Sperm in Different Age Groups for the Identification of Reproductive Function Proteins Anwar, P.; Maulana, T.; Sumantri, C.; Arifiantini, I.; Gunawan, A.
Tropical Animal Science Journal Vol. 48 No. 5 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.5.393

Abstract

Pesisir cattle are native to Indonesia and originate from West Sumatra. It is known for its high environmental adaptability and can be further developed using a proteomic approach. The proteomic approach for testing the fertility of Pesisir bulls is an important factor in determining a potentially superior breed. Proteomic studies of reproduction in Pesisir bulls have not been widely conducted. This study aimed to identify and characterize functional sperm proteins in Pesisir bulls that are associated with reproductive processes. Semen samples were collected from 12 pesisir bulls aged 2–4 years and grouped into three age categories. Protein profile analysis was performed using polyacrylamide gel electrophoresis with 1D-SDS-PAGE, followed by proteolytic digestion of the proteins in the gel and protein identification using LC-MS/MS analysis. Protein functions were predicted based on analysis of biological annotations generated using UniProt, Venny, PANTHER, and STRING. Based on the evaluation of fresh semen, sperm motility in Pesisir bulls was found to increase with age. Proteomic analysis successfully identified 334 proteins in the sperm of Pesisir bulls. Among these, two proteins, ZPBP and SPACA3, were identified as involved in reproductive and fertilisation processes. Functionally, both proteins play crucial roles in acrosomal events during fertilisation. Gene Ontology analysis showed that most proteins in sperm are involved in various biological processes, including cellular activities, metabolic processes, and molecular functions related to catalytic activities. In conclusion, SPACA3 and ZPBP proteins were identified as potentially involved in reproductive processes and may serve as fertility markers in Pesisir bulls.
Integrated Metabolomic and Functional Assessment of Sexed Frozen Semen in Holstein Friesian Bulls Yusuf, M.; Diansyah, A. M.; Sahiruddin, S.; Masturi, M.; Maulana, T.; Said, S.; Rahmat, R.; Alfian, A. M.; Adam, A. A. S.; Yusri, A. N. H. S.; Nurlatifah, A.; Amrullah, M. F.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.19

Abstract

This study examined the differences in sperm quality and metabolite profiles among X and Y sperm in Friesian Holstein bulls. It also tried to find specific metabolites that can help improve the accuracy of sexed semen use in humid tropical dairy farms. Semen samples from five bulls were separated by sex and tested for movement, live sperm count, shape, and membrane and acrosome health. The tests used Computer-Assisted Sperm Analysis (CASA) and regular microscope checks. Metabolite analysis was performed using LC-HRMS, followed by various statistical tests, pathway checks, and ROC curve analysis. The outcomes showed that X sperm had better movement and acrosome health, while Y sperm had stronger membranes, fewer shape problems, and moved faster. The untargeted metabolite study showed clear differences between the two groups. X sperm had more identified metabolites and greater average levels. The crucial metabolites found more in X sperm were D-(−)-fructose, L-(+)-lactic acid, and sn-glycero-3-phosphocholine. Meanwhile, Y sperm had greater levels of acetyl-L-carnitine. The pathway analysis showed that X sperm primarily utilized carbohydrate and fat processing through glycolysis and phosphocholine pathways to maintain their movement and membrane integrity. On the other hand, Y sperm mainly use a carnitine-based energy pathway to support their faster and straighter movement. The identified metabolites can serve as reliable biomarkers to aid in laboratory quality control checks and enhance the quality of semen extenders. This outcome gives a good way to make sexed semen more stable and reliable in tropical dairy farming conditions.
Co-Authors A. GUNAWAN Adam, A. A. S. Alfian, A. M. Alfiana Alfiana Amrullah, M. F. Anwar, P. C. SUMANTRI Christopher, E. Diansyah, A. M. F. Afiati, F. I. Arifiantini Ibrahim, G.E. M. A. Setiadi, M. A. M. Yusuf Masturi Masturi Nurlatifah, A. R. I. Arifiantini Rahmat, R. Ramadhan, M.N. S. Said Sahiruddin, S. Saputra, Irwan Setiorini, S. Usman, Said Yusri, A. N. H. S. Z, Nasrul