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CYP2A6 Gene Polymorphism Allele *4 Study in Hypertensive Patients with a History of Smoking Rahayu Sudarsono, Erica Kusuma; Christine Patramurti
Eureka Herba Indonesia Vol. 4 No. 4 (2023): Eureka Herba Indonesia
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/ehi.v4i4.91

Nicotine is a specific CYP2A6 substrate found in cigarettes. Nicotine levels in the blood are affected by the metabolic rate of the CYP2A6 enzyme, which is known to have a high level of polymorphism. High levels of nicotine in the blood can result in an increased risk of cardiovascular disease through the mechanism of increasing lipolysis and causing an increase in triglyceride levels in the blood. This study is an observational study conducted on 31 hypertensive men with a history of smoking. The purpose of this study was to determine the frequency of the CYP2A6 allele *4 genes in hypertensive patients with a history of smoking. Result analysis was performed on PCR products using electrophoresis, then the frequency of each allele was determined. The results showed that there was a CYP2A6 allele *4 gene in hypertensive patients with a history of smoking in Yogyakarta with an allele frequency of 62.90%, and only 2 respondents (6.45%) had the CYP2A6*1/*1 genotype or wildtype.

PEMERIKSAAN PROFIL LIPID PADA JEMAAT LANSIA SALAH SATU GEREJA KRISTEN DI YOGYAKARTA Patramurti, Christine; Triwulandari, Erna; Nastiti, Christofori Maria Ratna Rini
ABDIMAS ALTRUIS: Jurnal Pengabdian Kepada Masyarakat Vol 6, No 2 (2023): Oktober 2023
Publisher : Universitas Sanata Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/aa.v6i2.6829

Lipid profile examination including total cholesterol level, triglycerides level and high-density lipoprotein cholesterol reduction is essential to be done as a routine activity quinquennially to prevent someone from the risk of cardiovascular diseases, diabetes and obesity. This kind of examination is mandatory for the elderly people to maintain their quality of life. In order to support the government program on the prevention of the cardiovascular diseases, a team of social service of Faculty of Pharmacy Universitas Sanata Dharma (FFUSD) was organizing an event of lipid profile examination in one of Christian church located in Kalasan, Yogyakarta. As many as 35 elderly people were enthusiastically participating on the event. A nurse from Poliklinik Sanata Dharma was appointed to take the blood samples, which were further taken to the Biochemistry Laboratory in FFUSD to get analysed. The results were sent to the participants in private. The overall blood lipid profiles of most participants showed good level of quality, indicating that they have been trying to manage their life healthily. However, as much as 62. 8% of participants showed a low level of the high-density lipoprotein cholesterol. This was believed due to the food intake which was still rich in oil and fat. The event was very succesful and the responses were very positive. The participants felt assisted with a fast and accurate results sent.

The Genetic Polymorphisms of CYP3A4*1G and CYP3A5*3 in Javanese Indonesian Population: The Genetic Polymorphisms of CYP3A4*1G and CYP3A5*3 in Javanese Indonesian Atmaja, Sarah; Ellsya Angeline Rawar; Ani Kristiyani; Dwi Aris Agung Nugrahaningsih; Ahmad Hamim Sadewa; Christine Patramurti
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.06

Polymorphisms of CYP3A4*1G and CYP3A5*3 affect the pharmacokinetic profile of various drugs, e.g., fentanyl, tacrolimus, diltiazem, simvastatin. Tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR) is a simple and economical method for SNP determination. The polymorphisms in the CYP3A4*1G and CYP3A5*3 genes have not yet been examined using this method in Javanese Indonesian. Our aim was to determine the frequency of polymorphisms in the CYP3A4*1G and CYP3A5*3 genes in Indonesian Javanese using the ARMS-PCR method. Eighty-six patients at the Kalasan Community Health Centre in Yogyakarta, Indonesia, were chosen based on the inclusion criteria, which is Javanese ancestry. They gave their informed consent to blood collection by completing a form. Genetic variants were detected using Tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR). The chi-square test was used to determine genotype deviations from Hardy-Weinberg equilibrium, with a significant threshold of 0.05. For homozygous wild types, CYP3A4 *1/*1 dominated overall among study participants (73.35%), whereas for CYP3A5*3/*3, homozygous mutants were more prevalent (83.72%). Hardy-Weinberg equilibrium is consistent with genotype frequencies (p > 0.005). One participant carried a homozygous mutation for both CYP3A4*1G and CYP3A5*3, while the other 49 subjects were heterozygous for CYP3A4*1G and homozygous mutant for CYP3A5*3, which is the highest number of SNP combinations. The findings of the current investigation demonstrate that the population has the highest proportion of homozygous CYP3A4*1G wild-types (CYP3A4*1/*1) and homozygous mutants for CYP3A5*3 (CYP3A5*3/*3)

Edukasi Kebersihan Diri Bagi Siswa-Siswi SD Negeri 2 Salakan Banguntapan Bantul DIY Patramurti, Christine; Nastiti, C.M Ratna Rini; Primawan, A. Bayu
Jurnal Pengabdian Masyarakat Farmasi : Pharmacare Society Vol 4, No 1 (2025)
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/phar.soc.v4i1.30947

Menjaga kebersihan diri atau personal hygiene merupakan hal yang sangat penting terutama untuk menjaga diri tetap sehat, dan mengurangi risiko diri maupun orang terdekat terserang penyakit. Penamaman pola kebersihan diri ini harus dimulai sejak dini, bahkan dari usia batita. Oleh karena itu kegiatan ini bertujuan untuk mengajarkan siswa-siswi akan pentingnya menjaga dan memperhatikan mereka, sehingga bisa lebih fokus dalam belajar. Dalam upaya meningkatkan pengetahuan, perilaku, dan kualitas hidup siswa-siswi di SD Negeri 2 Salakan pada hari Jumat, 31 Mei 2024 telah dilakukan sosialisasi dan pelatihan pentingnya menjaga kebersihan diri bagi siswa-siswi SD Negeri 2 Salakan, Banguntapan, Bantul DIY. Pelaksanaan dimulai dengan pemberian kuis tentang kebersihan diri dan dilanjutkan dengan ceramah tentang kebersihan diri untuk seluruh siswa. Secara umum, siswa-siswi sangat antusias dalam mengikuti kegiatan ini. Berdasarkan kegiatan yang telah dilakukan dapat disimpulkan bahwa tingkat pengetahuan siswa-siswi sudah sangat baik. Siswa-siswa juga dapat mempraktekkan cara-cara menjaga kebersihan diri sesuai arahan yang diberikan saat kegiatan berlangsung. Dampak yang diharapkan dari kegiatan ini adalah siswa-siswi bisa menularkan kebiasaan baik mereka kepada teman-teman. Kegiatan ini juga dihadiri oleh semua guru SD Negeri 2 Salakan, melalui Kepala Sekolah, guru-guru berharap kegiatan ini dapat mengubah perilaku siswa-siswi menjadi lebih baik lagi, terutama dalam menjaga kebersihan diri, sehingga mereka bisa lebih fokus dalam mengikuti kegiatan belajar mengajar di kelas

The Genetic Polymorphisms of CYP3A4*1G and CYP3A5*3 in Javanese Indonesian Population: The Genetic Polymorphisms of CYP3A4*1G and CYP3A5*3 in Javanese Indonesian Atmaja, Sarah; Ellsya Angeline Rawar; Ani Kristiyani; Dwi Aris Agung Nugrahaningsih; Ahmad Hamim Sadewa; Christine Patramurti
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.06

Polymorphisms of CYP3A4*1G and CYP3A5*3 affect the pharmacokinetic profile of various drugs, e.g., fentanyl, tacrolimus, diltiazem, simvastatin. Tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR) is a simple and economical method for SNP determination. The polymorphisms in the CYP3A4*1G and CYP3A5*3 genes have not yet been examined using this method in Javanese Indonesian. Our aim was to determine the frequency of polymorphisms in the CYP3A4*1G and CYP3A5*3 genes in Indonesian Javanese using the ARMS-PCR method. Eighty-six patients at the Kalasan Community Health Centre in Yogyakarta, Indonesia, were chosen based on the inclusion criteria, which is Javanese ancestry. They gave their informed consent to blood collection by completing a form. Genetic variants were detected using Tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR). The chi-square test was used to determine genotype deviations from Hardy-Weinberg equilibrium, with a significant threshold of 0.05. For homozygous wild types, CYP3A4 *1/*1 dominated overall among study participants (73.35%), whereas for CYP3A5*3/*3, homozygous mutants were more prevalent (83.72%). Hardy-Weinberg equilibrium is consistent with genotype frequencies (p > 0.005). One participant carried a homozygous mutation for both CYP3A4*1G and CYP3A5*3, while the other 49 subjects were heterozygous for CYP3A4*1G and homozygous mutant for CYP3A5*3, which is the highest number of SNP combinations. The findings of the current investigation demonstrate that the population has the highest proportion of homozygous CYP3A4*1G wild-types (CYP3A4*1/*1) and homozygous mutants for CYP3A5*3 (CYP3A5*3/*3)

Edukasi Pencegahan Penyakit Tidak Menular Bagi Mahasiswa Generasi “Z” Melalui Gerakan Jalan Kaki Patramurti, Christine
Jurnal Pengabdi Vol 8, No 2 (2025): Oktober 2025
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jplp2km.v8i2.100437

Jalan kaki, merupakan jenis olah raga ringan yang dapat dilakukan oleh semua kalangan. Aktivitas jalan kaki yang dilakukan secara rutin setiap hari selama 30-60 menit dapat mengurangi risiko terkena PTM. Meskipun demikian, belum semua orang dapat menerapkan gaya hidup sehat ini dalam kehidupan sehari-hari. Perkembangan teknologi yang semakin cepat, menyebabkan budaya hidup instan mendominasi gaya hidup manusia pada jaman sekarang. Selain itu, perkembangan teknologi ini meningkatkan gaya hidup sedentary di kalangan masyarakat. Salah satu kelompok di masyarakat yang sangat lekat dengan budaya instan dan sedentary adalah generasi Z. Kegiatan ini bertujuan untuk melakukan edukasi tentang pentingnya melakukan aktivitas fisik sebagai salah satu cara mengurangi risiko PTM. Metode yang digunakan dalam kegiatan ini adalah diskusi interaktif, di mana peserta diajak untuk secara aktif berbagi pendapat dan pengalaman. Selain itu, pada kegiatan ini juga diberikan pre-tes dan post-tes untuk mengukur tingkat pemahaman peserta sebelum dan sesudah edukasi tentang gaya hidup sedentari dan PTM. Pada kegiatan ini juga dilakukan pengukuran indeks massa tubuh (IMT), tingkat aktivitas fisik (METs), serta lama waktu duduk harian bagi setiap peserta. Secara keseluruhan, kegiatan ini telah berhasil meningkatkan pemahaman dan kesadaran di kalangan peserta tentang pentingnya mengurangi gaya hidup sedentari melalui aktivitas sederhana seperti jalan kaki. Peserta menunjukkan antusiasme tinggi dan berkomitmen untuk mengintegrasikan kebiasaan sehat ini ke dalam kehidupan sehari-hari mereka. Para peserta juga termotivasi untuk meningkatkan aktivitas fisik mereka untuk mencegah risiko PTM.

Genetic CYP2A6 Polymorphism May Worsen Glycohemoglobin Levels: Study among Javanese Indonesian Smokers Patramurti, Christine; Virginia, Dita Maria
Borneo Journal of Pharmacy Vol. 7 No. 1 (2024): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v7i1.5467

We have examined the inactive CYP2A6 alleles gene, including CYP2A6*4, CYP2A6*7, and CYP2A6*9, associated with glycohemoglobin levels among Javanese Indonesian smokers. There are 106 smokers participating in this study. Due to the number of cigarettes smoked per day, there are three groups of smokers: light, intermediate, and heavy smokers, with 98.7% being light and intermediated smokers while the rest are heavy smokers. All participants had smoked for more than 10 years, indicating they had been exposed to nicotine for a long time. Based on their genotype, there were four groups of smokers, including fast, intermediate, slow, and poor metabolizers. Most fast and intermediate metabolizers have HbA1c levels in the normal range (<5.7). On the other hand, most slow metabolizers have Hb1c levels >5.7, and all fast metabolizers have HbA1c levels >5.7, indicating that they the prediabetes and diabetes. The chi-square test showed a relationship between CYP2A6 polymorphism and HbA1c levels among the participants (P-value 0.000 <0.005 and χ2=54.6, df=1). The presence of an inactive allele will worsen the HbA1c levels in smokers.

Smoking as a risk factor for dyslipidemia: study among type 2 diabetes mellitus patients on private hospital in Yogyakarta Patramurti, Christine; Virginia, Dita Maria
JURNAL ILMU KEFARMASIAN INDONESIA Vol 23 No 2 (2025): JIFI
Publisher : Faculty of Pharmacy, Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v23i2.1720

Smoking behavior is known to be a significant factor that has adverse effects on people with type 2 diabetes (T2DM). Nicotine in cigarettes stimulate adrenoreceptors and promotes lipolysis, leading to the synthesis of free fatty acids, which in turn increase the susceptibility to dyslipidemia and the development of cardiovascular disorders. This study aimed to examine the relationship between smoking status and the occurrence of dyslipidemia in patients with T2DM in a private hospital in Yogyakarta. A total of 107 T2DM patients participated in this cross-sectional observational study. Through live interviews, we collected smoking status conducted using the Fagerström Test for Nicotine Dependence (FTND) questionnaire. The fasting blood samples taken from participants were used to analyze the lipid profiles, including triglycerides (TG), total cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein (HDL). The chi-square test revealed a significant association between smoking status and the risk of dyslipidemia among the participants, with χ²(2) = 13.463, p = 0.001. The findings suggest that smoking increases the risk of dyslipidemia among T2DM patients. These results highlight the importance of smoking cessation as a preventive measure to reduce metabolic complications in individuals with T2DM.

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