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All Journal Journal of Tropical Biodiversity and Biotechnology ZOO INDONESIA Jurnal Entomologi Indonesia
Warsito Tantowijoyo, Warsito
Pusat Penelitian Biologi Jln. Raya Bogor Km. 46, Cibinong 16916
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Other

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Parasitoid serangga pengorok daun Liriomyza: intensitas parasitisasi, perubahan sebaran dan komposisinya Erniwati, Erniwati; Lupiyanindyah, Pungki; Tantowijoyo, Warsito
ZOO INDONESIA Vol 22, No 1 (2013): Juli 2013
Publisher : Masyarakat Zoologi Indonesia

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Abstract

Serangga invasif lalat pengorok daun merupakan hama penting berbagai tanaman sayuran. Serangga ini berasosiasi dengan berbagai musuh alami sebagai agen pengendali populasinya. Penelitian ini bertujuan mengetahui potensi, perubahan sebaran dan komposisinya. Komposisi dan populasi musuh alami diamati dengan metode sampling daun yang terserang. Di masing-masing titik sampling, koleksi daun yang terserang dilakukan pada semua jenis tanaman dan gulma. Parasitoid serangga pengorok daun Liriomyza lebih menyukai untuk memarasit inang yang menyerang tanaman kacang buncis (50%), ceisin (55%), kentang (57%), kacang babi (70%), dan kacang merah (75%). Intensitas parasitoid meningkat (10,4% - 74,5%) menurut ketinggian tempatnya mulai dari 200 – 2200 m dpl. Sebaran dan komposisi parasitoid berubah dimana Opius chromatomyiae ditemukan di dataran rendah dan lebih dominan dibanding Hemiptarsenus varicornis. Opius chromatomyiae di dataran rendah mempunyai ukuran toraks yang lebih kecil
BahaKajian aspek keamanan nyamuk Aedes aegypti Linnaeus ber-Wolbachia di Yogyakarta, Indonesiasa Indonesia: Assessing the safety of Wolbachia-infected Aedes aegypti Linnaeus mosquitoes in Yogyakarta, Indonesia Saraswati, Utari; Supriyati, Endah; Rahayu, Ayu; Rovik, Anwar; Kurniasari, Irianti; Hermantara, Rio; Kumalawati, Dian Aruni; Daniwijaya, Edwin Widyanto; Fitriana, Iva; Pramuko, Nida Budiwati; Indriani, Citra; Wardana, Dwi Satria; Tantowijoyo, Warsito; Ahmad, Riris Andono; Utarini, Adi; Arguni, Eggi
Jurnal Entomologi Indonesia Vol 20 No 2 (2023): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.2.117

Abstract

Dengue prevention efforts are limited to the control strategies of its vector and the management of breeding sites. New alternatives for dengue vector control that are sustainable and more environmentally friendly are needed to complement the government's current efforts. Research on Wolbachia-infected Aedes aegypti Linnaeus mosquitoes as an alternative biocontrol strategy has been performed in Yogyakarta City. However, one of the concerns of the community members and stakeholders about this technology is the safety aspect regarding the transmission of Wolbachia to other species and the possibility that humans will contract Wolbachia. This study aimed to address these concerns, namely to find out whether horizontal transmission of Wolbachia occurred from A. aegypti that were released to other species and whether residents living in the released areas were infected with Wolbachia. The research was conducted in Dusun Nogotirto and Dusun Kronggahan (Sleman Regency), as well as in Dusun Jomblangan and Dusun Singosaren (Bantul Regency), Yogyakarta Special Province. Wolbachia qPCR screening using the target gene WD0513 was performed on 922 Culex quinquefasciatus Say and 331 Aedes albopictus (Skuse). ELISA test was carried out on 190 pairs of plasma samples, namely the sample before the Wolbachia frequency was established (still <80%) and the sample after it was established (>80%). The results showed no evidence of Wolbachia transfer from Wolbachia-infected A. aegypti to other mosquito species coexisting in the same habitat or to humans. This study corroborates the safety evidence of Wolbachia-infected A. aegypti technology as an alternative to control dengue virus transmission
Lessons from the Mass Production of Wolbachia-infected Aedes aegypti for Egg Release in the Sleman and Bantul Districts of Yogyakarta Fitriana, Iva; Nurhayati, Indah; Arianto, Budi; Chusnaifah, Defriana Lutfi; Utami, Indira Diah; Purwaningrum, Nabhela Ayu; Saraswati, Utari; Supriyati, Endah; Utarini, Adi; Ahmad, Riris Andono; Indriani, Citra; Arguni, Eggi; Tantowijoyo, Warsito
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84753

Abstract

An efficacy study on wMel Wolbachia-infected Aedes aegypti technology conducted by the World Mosquito Program (WMP) Yogyakarta showed the reducing of dengue incidence in Yogyakarta City. Following this successful result, the intervention was scaled up into two neighbouring districts: Sleman and Bantul. This paper describes our experience in mass production for providing release material for a larger area to reach the deployment target, which includes insectary requirements, mass production protocols, and diagnostic screening. This review may serve as a reference guidance for national mass production for wMel Wolbachia-infected Ae. aegypti.  
Co-Authors Adi Utarini Ayu Rahayu Budi Arianto, Budi Chusnaifah, Defriana Lutfi Citra Indriani Daniwijaya, Edwin Widyanto DIAN ARUNI KUMALAWATI Eggi Arguni Erniwati Erniwati Fitriana, Iva Indah Nurhayati Kurniasari, Irianti Pramuko, Nida Budiwati Pungki Lupiyanindyah, Pungki Purwaningrum, Nabhela Ayu Rio Hermantara Riris Andono Ahmad Rovik, Anwar Saraswati, Utari Supriyati, Endah Utami, Indira Diah Wardana, Dwi Satria