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All Journal Indonesian Journal of Biotechnology Jurnal Entomologi Indonesia Narra J Indonesian Journal of Life Sciences
Rio Hermantara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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Induced-Coagulated Plasma-Fibrin Gels as a Biological Scaffold for Cell Attachment and Proliferation of Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSC) Rio Hermantara; Fiano A. Kerans; Rizal R; E. Henny Herningtyas; Lutfan Lazuardi
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.872 KB) | DOI: 10.22146/ijbiotech.9310

Abstract

Fibrin gels are an ideal natural biological scaffold for tissue engineering because they are biocompatible,biodegradable, and have many biological surface markers. However, most research on fi brin gels used commercialfi brin kits that could be costly and limited in some areas. In this study, fi brin gels were made by inducing bloodcoagulation by adding a common diagnostic kit to assess the time for blood to clot, called activated partialthromboplastin time (aPTT). This induced coagulated plasma (iCoplas)-fi brin gels was evaluated for its ability toenhance biological activity of umbilical cord-derived mesenchymal stem cell (UC-MSC), which were cell attachmentand proliferation. Fibrinogen concentration had infl uence on cell attachment, where only 50% of the cells couldattach to 77 mg/dl fi brinogen gels whereas 93% cells adhered to 154 mg/dl fi brin gels. There were no signifi cantdifferences in cell proliferation on polysterene culture dish and fi brin gels (p>0.05). These results showed thatiCoplas-fi brin gels could be used as a fi brin-based scaffold, yielding no signifi cant difference than polysterene-tissueculture dish cultures in cell attachment and cell proliferation on 154 mg/dl fi brinogen concentration.
BahaKajian aspek keamanan nyamuk Aedes aegypti Linnaeus ber-Wolbachia di Yogyakarta, Indonesiasa Indonesia: Assessing the safety of Wolbachia-infected Aedes aegypti Linnaeus mosquitoes in Yogyakarta, Indonesia Saraswati, Utari; Supriyati, Endah; Rahayu, Ayu; Rovik, Anwar; Kurniasari, Irianti; Hermantara, Rio; Kumalawati, Dian Aruni; Daniwijaya, Edwin Widyanto; Fitriana, Iva; Pramuko, Nida Budiwati; Indriani, Citra; Wardana, Dwi Satria; Tantowijoyo, Warsito; Ahmad, Riris Andono; Utarini, Adi; Arguni, Eggi
Jurnal Entomologi Indonesia Vol 20 No 2 (2023): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.2.117

Abstract

Dengue prevention efforts are limited to the control strategies of its vector and the management of breeding sites. New alternatives for dengue vector control that are sustainable and more environmentally friendly are needed to complement the government's current efforts. Research on Wolbachia-infected Aedes aegypti Linnaeus mosquitoes as an alternative biocontrol strategy has been performed in Yogyakarta City. However, one of the concerns of the community members and stakeholders about this technology is the safety aspect regarding the transmission of Wolbachia to other species and the possibility that humans will contract Wolbachia. This study aimed to address these concerns, namely to find out whether horizontal transmission of Wolbachia occurred from A. aegypti that were released to other species and whether residents living in the released areas were infected with Wolbachia. The research was conducted in Dusun Nogotirto and Dusun Kronggahan (Sleman Regency), as well as in Dusun Jomblangan and Dusun Singosaren (Bantul Regency), Yogyakarta Special Province. Wolbachia qPCR screening using the target gene WD0513 was performed on 922 Culex quinquefasciatus Say and 331 Aedes albopictus (Skuse). ELISA test was carried out on 190 pairs of plasma samples, namely the sample before the Wolbachia frequency was established (still <80%) and the sample after it was established (>80%). The results showed no evidence of Wolbachia transfer from Wolbachia-infected A. aegypti to other mosquito species coexisting in the same habitat or to humans. This study corroborates the safety evidence of Wolbachia-infected A. aegypti technology as an alternative to control dengue virus transmission
CRISPR-Cas9 Mediated Gene Therapy: Current Advancements and Applications Towards Tay-Sachs and Sandhoff Disease Josefano, Richelle Bertly; Yoel, Abigail; Japri, Beatrice Miranda; Belva, Felicia Lael; Widjojo, Clara Ayu; Hermantara, Rio
Indonesian Journal of Life Sciences 2024: IJLS Vol 06 No.02
Publisher : Indonesia International Institute for Life Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54250/ijls.v6i2.195

Abstract

Tay-Sachs disease and Sandhoff disease are neurodegenerative diseases that are classified as autosomal recessive lysosomal storage disorders. They are commonly caused by a mutation that occurs in the HEXA and HEXB genes, which are responsible for encoding beta-hexosaminidase-A (Hex A) and beta-hexosaminidase-B (Hex B). Furthermore, Sandhoff's disease symptoms include spinocerebellar ataxia, motor degeneration, sensorimotor neuropathy, tremor, dystonia, and psychosis, which are comparable to Tay-Sachs disease symptoms. The current treatment of Tay-sachs include enzyme replacement therapy, bone marrow transplantation, and administration of genetically modified stem cells with HexA which do not impede neurological dysfunction and were not effective in the long run. On the other hand, there is no standard treatment for Sandhoff but it utilizes bone marrow transplantation which is ineffective. So far, there is only one available gene editing treatment. Therefore, it might be necessary to consider gene editing as a prospective treatment for both diseases, with CRISPR being a primary method. By utilizing Adeno-associated viruses (AAV) as the delivery method for the CRISPR-Cas9 system, it can replace the defective HEXA or HEXB gene with a modified gene termed HEXM, which was found to be the gene codes for the Hex subunit of the same enzyme that is missing in Tay-Sachs and Sandhoff disease. Several challenges of implementing CRISPR-Cas9 technology to treat Tay-Sachs and Sandhoff disease include off-target mutations, unintentional cleavage of the non-targeted sites, and bioethical challenges. Further studies can be explored using various CRISPR-Cas9 systems to improve its efficiency.
CRISPR-Cas9: A Story of Discovery, Innovation, and Revolution in Genome Editing Hermantara, Rio
Indonesian Journal of Life Sciences 2024: IJLS Vol 06 No.01
Publisher : Indonesia International Institute for Life Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54250/ijls.v6i01.197

Abstract

CRISPR-Cas9 is a powerful and flexible genome editing technology that has transformed the field of life sciences. It enables the creation and modification of genomic sequences of various organisms, with wide-ranging implications for biotechnology, agriculture, and medical research. The origin of CRISPR as an adaptive immune system in bacteria, the elucidation of CRISPR-Cas9 biological and molecular mechanisms, and the subsequent engineering and optimization of it as a programmable genome editing tool are remarkable achievements in science. In this review, we will summarize this fascinating history and also explore the recent innovations of the CRISPR-Cas9 system, which extend the genomic tool box to improve the accuracy and efficiency of genome editing.
Designing hybrid CRISPR-Cas12 and LAMP detection systems for treatment-resistant Plasmodium falciparum with in silico method Parikesit, Arli A.; Hermantara, Rio; kevin, Gregorius; Sidhartha, Elizabeth
Narra J Vol. 3 No. 3 (2023): December 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i3.301

Abstract

Genes associated with drug resistance of first line drugs for Plasmodium falciparum have been identified and characterized of which three genes most commonly associated with drug resistance are P. falciparum chloroquine resistance transporter gene (PfCRT), P. falciparum multidrug drug resistance gene 1 (PfMDR1), and P. falciparum Kelch protein K13 gene (PfKelch13). Polymorphism in these genes could be used as molecular markers for identifying drug resistant strains. Nucleic acid amplification test (NAAT) along with DNA sequencing is a powerful diagnostic tool that could identify these polymorphisms. However, current NAAT and DNA sequencing technologies require specific instruments which might limit its application in rural areas. More recently, a combination of isothermal amplification and CRISPR detection system showed promising results in detecting mutations at a nucleic acid level. Moreover, the Loop-mediated isothermal amplification (LAMP)-CRISPR systems offer robust and straightforward detection, enabling it to be deployed in rural and remote areas. The aim of this study was to develop a novel diagnostic method, based on LAMP of targeted genes, that would enable the identification of drug-resistant P. falciparum strains. The methods were centered on sequence analysis of P. falciparum genome, LAMP primers design, and CRISPR target prediction. Our designed primers are satisfactory for identifying polymorphism associated with drug resistant in PfCRT, PfMDR1, and PfKelch13. Overall, the developed system is promising to be used as a detection method for P. falciparum treatment-resistant strains. However, optimization and further validation the developed CRISPR-LAMP assay are needed to ensure its accuracy, reliability, and feasibility
Co-Authors Adi Utarini Ayu Rahayu Belva, Felicia Lael Citra Indriani Daniwijaya, Edwin Widyanto DIAN ARUNI KUMALAWATI E. Henny Herningtyas Eggi Arguni Fiano A. Kerans Fitriana, Iva Japri, Beatrice Miranda Josefano, Richelle Bertly Kevin, Gregorius Kurniasari, Irianti Lutfan Lazuardi Parikesit, Arli A. Pramuko, Nida Budiwati Riris Andono Ahmad Rizal R Rovik, Anwar Saraswati, Utari Sidhartha, Elizabeth Supriyati, Endah Wardana, Dwi Satria Warsito Tantowijoyo, Warsito Widjojo, Clara Ayu Yoel, Abigail