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All Journal AGRIKAN Jurnal Ilmiah Agribisnis dan Perikanan Jurnal Biologi Tropis
Olan Rahayu Puji Astuti Nussa
Universitas Wijaya Kusuma Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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Aquaria flushing technique of rosella extract (Hibiscus sabdariffa Linn) to determine the value of SGOT and SGPT in milkfish (Chanos chanos) induced by heavy metals (Hg) Intan Permatasari Hermawan; Olan Rahayu Puji Astuti Nussa; Ady Kurnianto
Agrikan: Jurnal Agribisnis Perikanan Vol 15, No 2 (2022)
Publisher : Sangia Research Media and Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.agrikan.15.2.350-354

Abstract

The purpose of this study was to determine the value of SGOT and SGPT in milkfish induced by heavy metals (Hg) by aquaria flushing technique with Rosella (Hibiscus Sabdariffa Linn) extract. The samples used were 30 male adult milkfish divided into 5 groups. The treatment group was induced with a toxic dose of heavy metal mercury (Hg), which was dissolved in a volume of m3 of water. Blood collection from the heart vein of milkfish as much as 1-2 ml for SGPT and SGOT examination was carried out using the Berthelot method. The liver, gills and brain of milkfish were then examined, 300 grams were centrifuged until smooth and then dissolved in methanol to obtain concentrations of 25ppm, 50ppm, 100ppm, 200ppm, 300ppm, 400ppm, 500ppm, 600ppm, 700ppm, 800ppm, 9 00ppm. and 1000ppm then analyzed by ANOVA. Based on the research results of black roselle flower extract therapy with aquaria flushing system on male milkfish, the average value of SGOT and SGPT showed a very significant decrease in groups P1, P2, P3, and P4, against the P0 group or control group (P<0, 01 ).
Case Study of Pseudomonas sp. in Giant Gourami (Osphronemus goramy) via Anatomical Pathology, Histopathology, and Microbiological Examinations, and Tetracycline Sensitivity Testing Reina Puspita Rahmaniar; Indra Rahmawati; Olan Rahayu Puji Astuti Nussa; Muhammad Irwan Firmansyah
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12956

Abstract

Pseudomonas sp. is an opportunistic bacterial pathogen causing systemic infections in cultured fish, resulting in economic losses and increasing concerns regarding antimicrobial resistance. However, comprehensive pathological and microbiological confirmation remains limited. This study aimed to evaluate Pseudomonas sp. infection in giant gourami (Osphronemus goramy) through pathological examination, bacterial identification, and antibiotic susceptibility testing. A descriptive case study was conducted on one naturally infected fish (code F-11). Clinical observation, necropsy, histopathological examination of the liver and gills using hematoxylin-eosin staining, bacterial isolation on MacConkey agar, Gram staining, biochemical characterization, and Kirby-Bauer disk diffusion testing with 30 μg tetracycline were performed. Data were analyzed descriptively by integrating pathological and microbiological findings. Gross examination revealed ecchymotic hemorrhages in the gills, labyrinth organ, and liver, hepatic nodules, and jejunal petechiae. Histopathology demonstrated congestion, inflammatory cell infiltration, hemorrhage, focal hepatic necrosis, epithelial desquamation, and lamellar necrosis in the gills. The isolate produced transparent non-lactose-fermenting colonies, consisted of Gram-negative rods, and exhibited biochemical characteristics consistent with Pseudomonas sp. The isolate remained susceptible to tetracycline, producing a 28-mm inhibition zone. These findings confirm Pseudomonas sp. infection and highlight the importance of integrated pathological and microbiological diagnosis to support appropriate antimicrobial therapy and antimicrobial resistance surveillance in aquaculture.
Co-Authors Indra Rahmawati Intan Permatasari Hermawan Kurnianto, Ady Muhammad Irwan Firmansyah Rahmaniar, Reina Puspita