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Rahmaniar, Reina Puspita
Universitas Wijaya Kusuma Surabaya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Resistansi Antibiotik Secara Fenotip Dan Deteksi Gen TetA pada Sampel Hati Ayam di Pasar Dukuh Kupang Surabaya Rahmaniar, Reina Puspita; Widhowati, Dyah; Hidayah, Nurul
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol 11 No 3 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Vete
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v11i3.244

Abstract

Abstract The purpose of this study was to determine the resistance of several antibiotics phenotypically and genotypically to detect the tetA gene from broiler chicken liver samples at Dukuh Kupang market, Surabaya. A total of 30 samples were taken and then prepared aseptically and sterile. Isolation on Eosin Methylene Blue Agar (EMBA) media, then microscopic examination using gram staining and biochemical tests of Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Methyl Red (MR), Voges Prouskauers (VP) and Simons Citrate Agar (SCA). The identified Escherichia coli colonies were tested for antibiotic sensitivity using the Kirby Bauer method, then isolates that were proven to be resistant to tetracycline antibiotics were followed by genetic testing using the Polymerase Chain Reaction (PCR) method. The results showed that 20 of the 30 samples were positive for Escherichia coli. Escherichia coli isolates from chicken liver samples showed resistance to 30 µg tetracycline antibiotics by 85% (17 of 20 samples) Researchers also compared with other antibiotics, the highest resistance to ampicillin 10 µg was 90% (18 out of 20 samples), gentamicin resistance was 10 µg by 50% (10 of 20 samples) and 30 µg chloramphenicol antibiotic resistance by 30% (6 of 20 samples). The isolates that were resistant to tetracycline were confirmed by Polymerase Chain Reaction to detect the tetA gene with the final product in the form of a band with a length of 210 bp. Bacterial isolates resistant to Tetracycline antibiotics did not always show TetA gene expression in the PCR test. Keywords: Antibiotic Resistance; Escherichia coli; Market; TetA gene Abstrak Tujuan dari penelitian ini yaitu untuk mengetahui resistansi beberapa antibiotik secara fenotip dan secara genotip mendeteksi gen tetA dari sampel hati ayam broiler di pasar Dukuh Kupang Surabaya. Sebanyak 30 sampel diambil kemudian dipreparasi secara aseptis dan steril. Isolasi pada media Eosin Methilen Blue Agar (EMBA), selanjutnya dilakukan pemeriksaan mikroskopis menggunakan pewarnaan gram dan uji biokimiawi Triple Sugar Iron Agar (TSIA), Sulfide Indol Motility (SIM), Methyl Red (MR), Voges Prouskauers (VP), dan Simons Citrat Agar (SCA). Koloni Escherichia coli yang teridentifikasi dilakukan uji sensitifitas antibiotik dengan metode Kirby bauer, selanjutnya isolat yang terbukti resistan terhadap antibiotik tetrasiklin dilanjutkan pemeriksaan genetik dengan metode Polymerase Chain Reaction (PCR). Hasil penelitian menunjukkan bahwa 20 dari 30 sampel positif Escherichia coli. Isolat Escherichia coli asal sampel hati ayam menunjukkan resistansi terhadap antibiotik Tetrasiklin 30 µg sebesar 85 % (17 dari 20 sampel) Peneliti juga melakukan perbandingan dengan antibiotik lainnya, resistensi tertinggi pada antibiotik ampisilin 10 µg sebesar 90 % (18 dari 20 sampel), resistensi gentamisin 10 µg sebesar 50 % (10 dari 20 sampel) dan resistensi antibiotik kloramfenikol 30 µg sebesar 30 % (6 dari 20 sampel). Isolat yang resisten terhadap tetrasiklin dikonfirmasi dengan Polymerase Chain Reaction untuk mendeteksi gen tetA dengan produk akhir berupa band dengan panjang 210 bp. Isolat bakteri yang resistan terhadap antibiotik Tetrasiklin tidak selalu menunjukkan ekspresi gen tetA pada uji PCR. Kata kunci: Escherichia coli; Gen TetA; Pasar; Resistansi Antibiotik.
Perbedaan kadar protein, kadar lemak dan nilai pH susu sapi pada daerah dataran tinggi dan dataran rendah di Kabupaten Jombang Reina Puspita Rahmaniar; Dina Wahyu Nur Aini; Ratna Widyawati; Arif Mardjianto
Jurnal Sains Peternakan Vol 9 No 2 (2021): Jurnal Sains Peternakan
Publisher : Fakultas Peternakan, Universitas PGRI Kanjuruhan Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jsp.v9i2.6271

Abstract

ABSTRACT The purpose of this study was to determine the differences in protein content, fat content and pH value of cow's milk in the highlands and lowlands in Jombang Regency. A total of nine samples of cow's milk were taken from two cow milk shelters in different plains to be examined at the Veterinary Public Health Laboratory of the UWKS FKH. The protein content was tested by the formol method, the fat content was tested by the garber method and the pH value of milk was measured by a pH meter. The results showed that the average protein content in the highlands was 3.00% ± 0.012 and the lowlands was 3.20% ± 0.063, and the mean fat content in the highlands was 4.004% ± 0.063 and the lowlands 3.125% ± 0.109, the average pH value in the highlands was 6.7 ± 0.005 and the lowlands was 6.6 ± 0.043, so that the results of the comparison of cow's milk from the highlands and lowlands were analyzed using T test can be seen that there was no significant difference in the pH value and protein content, but in fat content there was a significant difference. Keywords: Cow's Milk, Protein Content, Fat Content, pH Value, Jombang
Antibakteri Nano Silver Terhadap Bakteri Escherichia coli Yang Diisolasi Dari Susu Sapi Mastitis Sheila Marty Yanestria; Era Hari Mudji; Elsa Rosita; Reina Puspita Rahmaniar
Jurnal Ilmiah Fillia Cendekia Vol 5 No 2 (2020): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v5i2.1178

Abstract

Penelitian ini dilakukan untuk mengetahui efektivitas antibakteri nano silver pada bakteri Escherichia coli yang diisolasi dari susu sapi mastitis. Penelitian ini menggunakan 5 perlakuan dengan 5 kali ulangan. Pada kelompok P0- sebagai kontrol negatif menggunakan Aquades, kelompok P0+ sebagai kontrol positif dengan perlakuan Amoxicillin, kelompok P1: perlakuan dengan larutan nano silver konsentrasi 50 ppm, kelompok P2: perlakuan dengan larutan nano silver konsentrasi 100 ppm dan kelompok P3: perlakuan dengan larutan nano silver dengan konsentrasi 150 ppm. Pada penelitian ini, pengamatan dilakukan dengan mengukur diameter zona hambat pada media MHA (Muller Hinton Agar) yang sudah dibiakkan bakteri murni Escherichia coli. Data yang diperoleh berdasarkan analisis menggunakan ANOVA menunjukan perbedaan yang sangat signifikan (P<0,05) pada setiap kelompok perlakuan. Hasil penelitian menunjukkan nano silver memiliki sifat antibakteri yang lemah terhadap bakteri Escherichia coli yang diisolasi dari susu sapi mastitis.
Kasus Ornithobacterium rhinotracheale dari Sampel Trachea pada Beberapa Jenis Unggas Reina Puspita Rahmaniar; Nurul Hidayah; Dyah Widhowati
Jurnal Ilmiah Fillia Cendekia Vol 6 No 1 (2021): Jurnal Ilmiah Fillia Cendekia
Publisher : Universitas Islam Kadiri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32503/fillia.v6i1.1473

Abstract

The aim of this study was to report cases of respiratory tract infection caused by the bacterium Ornithobacterium rhinotracheale. Observations included anatomical pathology and bacterial isolation results. Poultry was necropsed, observed changes in organs, trachea samples were taken aseptically then isolated on Blood Agar, Chocholate Agar, MCA, microscopic examination and biochemical tests, including TSIA, SIM, SCA, Catalase, Urease, MR, VP. The results of the examination showed the presence of Ornithobacterium rhinotracheale bacteria, the results showed small, grayish colored colonies. Microscopic examination, gram-negative pleomorphic rod-shaped bacteria. Bacteria did not grow on MCA, there was no change in TSIA media, non-motile bacteria, the results of the indole test, SCA, MR and urease were negative. All four types of birds showed pathological changes, including hemorrhage in the trachea and lungs, but the air sacs were normal and did not show any specific lesions, only duck samples with code I-11 had changes in the air sacs. Based on the results of isolation and bacteria, it can detect the presence of Ornithobacterium rhinotracheale from several different types of poultry, including layer chickens, broilers, local ornamental chickens and ducks.
Resistansi Antibiotik Secara Fenotip Dan Deteksi Gen TetA pada Sampel Hati Ayam di Pasar Dukuh Kupang Surabaya: Phenotive Antibiotic Resistance And Detection of TetA Gene in Chicken Liver Samples At Dukuh Market, Kupang Surabaya Reina Puspita Rahmaniar; Dyah Widhowati; Nurul Hidayah
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 11 No. 3 (2021): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v11i3.244

Abstract

Abstract The purpose of this study was to determine the resistance of several antibiotics phenotypically and genotypically to detect the tetA gene from broiler chicken liver samples at Dukuh Kupang market, Surabaya. A total of 30 samples were taken and then prepared aseptically and sterile. Isolation on Eosin Methylene Blue Agar (EMBA) media, then microscopic examination using gram staining and biochemical tests of Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Methyl Red (MR), Voges Prouskauers (VP) and Simons Citrate Agar (SCA). The identified Escherichia coli colonies were tested for antibiotic sensitivity using the Kirby Bauer method, then isolates that were proven to be resistant to tetracycline antibiotics were followed by genetic testing using the Polymerase Chain Reaction (PCR) method. The results showed that 20 of the 30 samples were positive for Escherichia coli. Escherichia coli isolates from chicken liver samples showed resistance to 30 µg tetracycline antibiotics by 85% (17 of 20 samples) Researchers also compared with other antibiotics, the highest resistance to ampicillin 10 µg was 90% (18 out of 20 samples), gentamicin resistance was 10 µg by 50% (10 of 20 samples) and 30 µg chloramphenicol antibiotic resistance by 30% (6 of 20 samples). The isolates that were resistant to tetracycline were confirmed by Polymerase Chain Reaction to detect the tetA gene with the final product in the form of a band with a length of 210 bp. Bacterial isolates resistant to Tetracycline antibiotics did not always show TetA gene expression in the PCR test. Keywords: Antibiotic Resistance; Escherichia coli; Market; TetA gene Abstrak Tujuan dari penelitian ini yaitu untuk mengetahui resistansi beberapa antibiotik secara fenotip dan secara genotip mendeteksi gen tetA dari sampel hati ayam broiler di pasar Dukuh Kupang Surabaya. Sebanyak 30 sampel diambil kemudian dipreparasi secara aseptis dan steril. Isolasi pada media Eosin Methilen Blue Agar (EMBA), selanjutnya dilakukan pemeriksaan mikroskopis menggunakan pewarnaan gram dan uji biokimiawi Triple Sugar Iron Agar (TSIA), Sulfide Indol Motility (SIM), Methyl Red (MR), Voges Prouskauers (VP), dan Simons Citrat Agar (SCA). Koloni Escherichia coli yang teridentifikasi dilakukan uji sensitifitas antibiotik dengan metode Kirby bauer, selanjutnya isolat yang terbukti resistan terhadap antibiotik tetrasiklin dilanjutkan pemeriksaan genetik dengan metode Polymerase Chain Reaction (PCR). Hasil penelitian menunjukkan bahwa 20 dari 30 sampel positif Escherichia coli. Isolat Escherichia coli asal sampel hati ayam menunjukkan resistansi terhadap antibiotik Tetrasiklin 30 µg sebesar 85 % (17 dari 20 sampel) Peneliti juga melakukan perbandingan dengan antibiotik lainnya, resistensi tertinggi pada antibiotik ampisilin 10 µg sebesar 90 % (18 dari 20 sampel), resistensi gentamisin 10 µg sebesar 50 % (10 dari 20 sampel) dan resistensi antibiotik kloramfenikol 30 µg sebesar 30 % (6 dari 20 sampel). Isolat yang resisten terhadap tetrasiklin dikonfirmasi dengan Polymerase Chain Reaction untuk mendeteksi gen tetA dengan produk akhir berupa band dengan panjang 210 bp. Isolat bakteri yang resistan terhadap antibiotik Tetrasiklin tidak selalu menunjukkan ekspresi gen tetA pada uji PCR. Kata kunci: Escherichia coli; Gen TetA; Pasar; Resistansi Antibiotik.
Garlic As Antibacterial in Lactose Fermenter Enterobacteriaceae Reina Puspita Rahmaniar; Dyah Widhowati; Nurul Hidayah
JURNAL KAJIAN VETERINER Vol 11 No 1 (2023): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v11i1.11002

Abstract

This study attempts to investigate the antibacterial effectiveness of three varieties of garlic (single garlic, kating garlic, and black garlic) on the growth of Enterobacteriaceae bacteria (Escherichia coli, Enterobacter sp, Klebsiella sp. from chicken meat samples. Treatment Kating garlic, single garlic, and black garlic at concentrations of 20%, 40%, 60%, 80%, 90%, 100%. This study observed the inhibition zone on Muller Hinton Agar (MHA) media that had been planted with bacterial isolates. The inhibition test uses the disk diffusion method. The findings revealed that kating garlic and single garlic showed inhibition zones against Escherichia coli, Enterobacter sp., and Klebsiella sp. bacteria. Meanwhile, black garlic did not show an inhibition zone against E. coli bacteria, but showed an inhibition zone against Klebsiella sp., Enterobacter sp., and Kating garlic had the largest bacterial inhibition zone against E. coli, Klebsiella sp., Enterobacter sp., followed by single garlic.
PENCEGAHAN MASTITIS SUBKLINIS PADA PETERNAKAN SAPI PERAH DI BENDUL MERISI SURABAYA Reina Puspita Rahmaniar; Dyah Widhowati; Hana Cipka Pramuda Wardhani
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 6, No 7 (2023): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v6i7.2621-2627

Abstract

Tujuan dari kegiatan pengabdian Masyarakat ini untuk meningkatkan kualitas susu melalui pencegahan mastitis subklinis pada wilayah peternakan sapi perah di Bendul Merisi Surabaya. Urgensi dilakukan pengabdian ini yaitu atas dasar tidak adanya KUD yang mengontrol Kesehatan sapi perah di wilayah tersebut, penyakit mastitis subklinis mempengaruhi kualitas susu namun tidak dapat dilihat secara langsung oleh peternak, lokasi peternakan dekat dengan penduduk, sehingga perlu edukasi agar tidak mudah terjadi kontaminasi silang dari agen infeksi yang menyebabkan penyakit. Pelaksanaan pengabdian ini terdiri beberapa tahapan. Pertama dilakukan kontrol penyakit mastitis subklinis melalui pengujian CMT, kemudian dilakukan isolasi bakteri pada sampel susu. Tahap selanjutnya dilakukan penyuluhan mengenai pencegahan penyakit mastitis pada sapi perah serta penggunaan antiseptik dan desinfektan. Berdasarkan hasil uji CMT didapatkan bahwa empat sampel negatif dan sampel yang positif hanya sampai positif dua. Hasil pengujan isolasi bakteri menunjukkan adanya pertumbuhan koloni bakteri Escherichia coli. Sampel pada uji CMT positif tidak selalu menunjukkan adanya bakteri, begitu juga sampel yang dilakukan uji CMT dengan hasil negatif, terdapat pertumbuhan bakteri. Pengujian tersebut penting untuk dilakukan untuk Tindakan pencegahan dan pengendalian penyakit. Peternak di bendul merisi Surabaya harus menerapkan prinsif aseptis dan menggunakan desinfektan pada kandang
The Detection of Resistant Escherichia coli Isolated From Cats In Dukuh Kupang Sub-District, Surabaya Wibisono, Freshinta Jellia; Wirjaatmadja, Roeswandono; Candra, Adhitya Yoppy Ro; Rahmaniar, Reina Puspita; Trirahayu, Rizkika Amalia; Diningrum, Dinda Prisilya; Rianto, Vinsensius; Kendek, Irfan Alias
Acta VETERINARIA Indonesiana Vol. 12 No. 1 (2024): Maret 2024
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.12.1.1-7

Abstract

Antimicrobial resistance is a global issue that has become the center of world attention. Transmission to humans through pets is important to be aware of. This research was conducted to determine the presence of antimicrobial resistance in Escherichia coli bacteria in cats. A total of 60 swab samples were taken, consisting of 30 stray cats samples and 30 domestic cats samples. Samples were tested at the Veterinary Health Laboratory, Faculty of Veterinary Medicine, Wijaya Kusuma University, Surabaya. The samples were then isolated and identified using differential selective media Eosin Methylene Blue Agar. Escherichia coli isolates were then subjected to a sensitivity test to determine resistance to the antibiotics ampicillin, tetracycline and streptomycin. The results showed that 83% of the samples contained Escherichia coli bacteria in cats, with 16% antibiotic resistance to the antibiotic ampicillin, 19% in stray cats, and 12% in domestic cats. Resistance to tetracycline antibiotics shows 12%, where stray cats are 15%, and domestic cats are 8%. Meanwhile resistance to the antibiotic streptomycin showed 4% (2/50), with resistance in stray cats 8% and in domestic cats no resistance was found (0%). Cats, as animals that have a close relationship with human life, can be a factor in transmitting antimicrobial resistance, this is an early precaution as a measure to prevent the transmission of antimicrobial resistance to humans.
SENSITIVITAS ANTIMIKROBA TERHADAP BAKTERI ESCHERICHIA COLI YANG DIISOLASI DARI UDANG DI PASAR KEPUTRAN SURABAYA Rahmaniar, Reina Puspita; Widhowati, Dyah; Hidayah, Nurul
JURNAL KAJIAN VETERINER Vol 7 No 2 (2019): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v7i2.1978

Abstract

The aimed of this study was to identify antibiotic Resistance of Escherichia coli isolated from Shrimp. There were 20 samples taken and isolated on surface of Eosin Methilen Blue Agar. E.coli produced metalic green colonies. Samples identified as E.coli based on macroscopic features and morphology of colonies, microscopic examination with gram staining and biochemical tests. The antibiotic sensitivity was determined through a standard antimicrobial disk diffusion test. The results of this study showed that 9 samples were bacteria of the spesies E. coli. Based on the results of antibiotic sensitivity test, it was found that 100 % of E. coli isolates were resistant to Amoxycillin 20 µg and 22,2 % of isolates are resistant to ampicillin 10 µg. Antibiotics Tetracycline 30 µg and Chloramphenicol 30 µg were 100 % sensitive. Gentamicin 10 µg showed that 77,8 % sensitive and 22,2 % intermediet. The precence of antibiotic resistance of betalaktam antibiotics makes it especially important to monitor antimicrobial susceptibility and mechanisms of resistance of E.coli isolated from shrimp, because new mechanisms of resistance occurring in animals may enter the food chain and its be transferred to the human. This importance of cooperation between sectors in order to monitor antimicrobial resistance.
ISOLASI BAKTERI PENYEBAB PENYAKIT DARI KASUS FOWL CHOLERA PADA MERPATI (COLUMBIA LIVIA) Rahmaniar, Reina Puspita; Widhowati, Dyah; Ningsih, Awalul Fitria
BIOGENIC : Jurnal Ilmiah Biologi Vol 2 No 2 (2024): BIOGENIC: Jurnal Ilmiah Biologi
Publisher : Program Studi Biologi UNARS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/biogenic.v2i2.5771

Abstract

Tujuan dari penelitian ini yaitu untuk melakukan melakukan pemeriksaan patologi anatomi terhadap perubahan organ secara makroskopis dan isolasi dan identifikasi bakteri penyebab gejala fowl cholera pada merpati (Columba livia). Merpati mengalami gangguan pernafasan, keluar discharge nasal berwarna keruh, feses berbentuk pasta berwarna hijau, setelah dilakukan pemeriksaan terhadap tanda klinis yang muncul, selanjutnya dilakukan nekropsi dan pemeriksaan patologi anatomi serta isolasi bakteri dari best sample yang mengalami perubahan. Hasil pemeriksaan menunjukkan pulmo mengalami hemoragi dengan konsistensi rapuh. Hepar mengalami hemoragi, Jantung mengalami hiperemi. Hasil isolasi bakteri menunjukkan adanya bakteri Pasteurella multocida penyebab penyakit fowl cholera. Bakteri tumbuh pada blood agar (BA) dengan morfologi koloni berwarna abu-abu, tanpa hemolisa, pada mac conkey agar (MCA) tidak terdapat pertumbuhan bakteri, bakteri gram negatif, coccobasil, pada media triple sugar iron agar (TSIA), bagian slant acid, butt acid, gas dan H2S negatif, hasil pengujian sulfide indole motility (SIM) terdapat cincin merah artinya indol positif dan bakteri non motil, katalase positif, methyl red (MR) positif, simmon citrate agar (SCA), urease dan voges proskauer (VP) negatif. Disimpulkan bahwa terdapat bakteri Pasteurella multocida pada merpati dengan nomor sampel M-139.
Co-Authors Arif Mardjianto Candra, Adhitya Yoppy Ro Dina Wahyu Nur Aini Diningrum, Dinda Prisilya Elsa Rosita Era Hari Mudji Hana Cipka Pramuda Wardhani Kendek, Irfan Alias Ningsih, Awalul Fitria NURUL HIDAYAH Nurul Hidayah Nurul Hidayah Ratna Widyawati Rianto, Vinsensius Sheila Marty Yanestria Trirahayu, Rizkika Amalia Wibisono, Freshinta Jellia Widhowati, Dyah Wirjaatmadja, Roeswandono