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All Journal JURNAL ANALIS LABORATORIUM MEDIK Jurnal Ners BioEksakta : Jurnal Ilmiah Biologi Unsoed Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks
Neike Octary
Diploma III Study Program In Medical Laboratory Technology, STIKes Prima Indonesia, Bekasi, West Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Social Sciences Veterinary Other

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Liver Tissue Examination of Mice Using 10% BNF Fixation For 6 Hours And 16 Hours Octary, Neike; Sari, Indah; Aristoteles
Jurnal Analis Laboratorium Medik Vol 7 No 2 (2022): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v7i2.3457

Abstract

The fixation process is the first stage in the manufacture of histopathological preparations which aims to preserve the tissue and harden the tissue, so that the tissue to be observed does not change in shape or size. BNF fixation solution (Neutral Buffer Formalin) 10% has been used as a routine fixative and It has been be the gold standard in histology laboratories for decades. The material used in this study was a 10% BNF solution because it is easier to use and it can be used to preserve tissue for a long period of time. This study aims to determine the differences in the microscopic results of the liver tissue of mice (Mus musculus) fixed with 10% BNF for 6 hours and 16 hours. This type of research is descriptive analytic. The research was carried out at the Barokah Laboratory/ dr.Mezfi Unita on 04-05 March 2022 with a total sample of 20 samples of mice liver tissue (Mus musculus). The results of the study on 10% BNF fixation for 6 hours and 16 hours showed that the average results were not good. The conclusion from the results of the study that there was no difference in the microscopic results of liver tissue that were fixed with 10% BNF for 6 hours and 16 hours using Hematorylin-Eosin staining. 10% BNF fixation with a time of 6 hours and 16 hours can be used as an alternative in histopathological examination in the short term and long term.
Formulasi Sediaan Gargarisma Kombinasi Katekin Dengan Infusa Daun Sirih ( Piper betle L.) Serta Uji Aktivitas Antibakteri Terhadap Bakteri S.Mutans Reski Mulia; Neike Octary; Muhammad Fathurrahman
Jurnal Ners Vol. 10 No. 1 (2026): JANUARI 2026
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v10i1.53879

Abstract

Katekin merupakan senyawa golongan polifenol yang berpotensi sebagai antioksidan, antiinflamasi, obat diare, dan obat disentri. Sirih mengandung senyawa tanin dan minyak atsiri seperti hidroksikavikol, kavikol, kalibetol, allypyroketkol, karvakol, eugenol, cyneole, dan cadine, yang berpotensi sebagai antiinflamasi, antikejang, analgetik, anestetik, antidiare, dan antimutagenik. Senyawa ini terbukti dapat menghambat pertumbuhan bakteri S. mutans yang menginisiasi pembentukan plak sehingga dapat menimbulkan karies gigi. Penelitian ini bertujuan untuk membuat formulasi sediaan gargarisma kombinasi katekin dengan infusa daun sirih dan melihat aktivitas antibakteri terhadap bakteri S. mutans. Hasil evaluasi sediaan pada suhu ruang (25-30°C) menunjukan bahwa F1, F2, F3, F4 merupakan formula yang stabil dibandingkan dengan formula lain dan gargarisma X. Sediaan gargarisma yang mengandung katekin dan daun sirih memiliki daya hambat terhadap bakteri S. mutans ATCC 25175. Dari Penelitian ini dapat disimpulkan bahwa sediaan F1 merupakan sediaan yang memiliki diameter hambat paling besar dibandingkan sediaan lain dan gargarisma pembanding X
Potensi Ekstrak Cyperus rotundus Linn. sebagai Agen Antijamur Alami pada Kandidiasis Sharfina Maulida Yanti; Ishafani Ishafani; Ing Mayfa Br Situmorang; Neike Octary
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 4 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.4.17339

Abstract

Kandidiasis merupakan infeksi yang dapat bersifat primer maupun sekunder, disebabkan oleh Candida albicans, salah satu penyebab umum keputihan (fluor albus). Indonesia dengan iklim tropis memiliki keanekaragaman tanaman yang berpotensi sebagai obat alami, termasuk rumput teki (Cyperus rotundus Linn.) yang sering dianggap gulma. Umbi rumput teki mengandung senyawa kimia yang berpotensi sebagai antifungi alami. Penelitian ini bertujuan menguji potensi ekstrak umbi rumput teki terhadap C. albicans dengan berbagai konsentrasi. Penelitian bersifat deskriptif eksperimental menggunakan umbi rumput teki sebagai populasi. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 96% pada empat konsentrasi: 25%, 50%, 75%, dan 100%. Flukonazol digunakan sebagai kontrol positif dan CMC 1% sebagai kontrol negatif. Uji daya antifungi dilakukan menggunakan metode difusi cakram dan dilusi untuk menentukan Konsentrasi Hambat Minimum (KHM), dengan media Sabouraud Dextrose Agar (SDA) dan Sabouraud Dextrose Broth (SDB). Hasil penelitian menunjukkan diameter rata-rata zona hambat: konsentrasi 25% sebesar 6,75 mm; 50% sebesar 7 mm; 75% sebesar 12,75 mm; 100% sebesar 16 mm. Zona hambat kontrol positif (flukonazol) sebesar 22 mm, sedangkan kontrol negatif (CMC 1%) sebesar 0 mm. Standar deviasi rata-rata zona hambat adalah 7,79. KHM ekstrak umbi rumput teki terhadap C. albicans diperoleh pada konsentrasi 100%. Kesimpulannya, ekstrak umbi rumput teki memiliki aktivitas antifungi terhadap Candida albicans, dengan efektivitas meningkat seiring kenaikan konsentrasi, meskipun masih di bawah efektivitas flukonazol. Kata kunci : Antifungi, Candida albicans, Konsentrasi, KHM Rumput Teki,
Optimizing Environmental Hygiene through Education as a Strategy for Preventing Infectious Diseases at the An-Nuriyyah Orphanage Neike Octary; Asbar Tanjung; Endang Purwati; Reski Mulia; Gumilar Pratama; Umi Salamah; Juwy Trianes
Warta Pengabdian Andalas Vol 33 No 2 (2026)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.33.2.295-303.2026

Abstract

Orphanages, as communal living facilities, are vulnerable to the transmission of infectious diseases due to overcrowding, inadequate sanitation, and the low implementation of clean and healthy living behaviors. These conditions require health promotion and disease prevention interventions through educational programs to improve residents' knowledge, awareness, and hygiene practices. This community service program to improve the understanding of children and caregivers at An-Nuriyyah Orphanage regarding the importance of environmental hygiene in preventing infectious diseases. used to carry out the activities included health education, demonstrations of proper handwashing techniques, interactive discussions, question-and-answer sessions, and the use of posters as a visual communication medium. Success was assessed by comparing knowledge levels before and after the intervention. The results demonstrated a significant improvement in participants' knowledge, with the average score increasing from 39.0% before the intervention to 85.8% after the intervention, representing an increase of 46.8%. Participants also showed high enthusiasm and were able to correctly perform the recommended handwashing procedures. In conclusion, an educational approach combining health education, hands- on demonstrations, and active participation effectively improves participants' understanding and promotes the sustainable adoption of clean and healthy living behaviors in the orphanage environment.
Co-Authors Aristoteles Asbar Tanjung Endang Purwati Gumilar Pratama Indah Sari Ing Mayfa Br Situmorang Ishafani Ishafani Juwy Trianes Muhammad Fathurrahman Reski Mulia Reski Mulia Sharfina Maulida Yanti Umi Salamah