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All Journal Bioma : Berkala Ilmiah Biologi ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Ovozoa: Journal of Animal Reproduction Jurnal Pengabdian kepada Masyarakat Nusantara Cendana Medical Journal Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Jurnal Veteriner Nusantara Biodiversitas Journal of Biological Diversity
Yustinus Oswin Primajuni Wuhan
Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Optimalisasi Petunjuk Penggunaan Alat-Alat Laboratorium di Laboratorium Patologi Klinik Program Studi Kedokteran Hewan Universitas Nusa Cendana Yustinus Oswin Primajuni Wuhan; Dede Rival Novian
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 3 No. 2 (2022): Jurnal Pengabdian kepada Masyarakat Nusantara (JPkMN)
Publisher : Cv. Utility Project Solution

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.506 KB) | DOI: 10.55338/jpkmn.v3i2.420

Abstract

Mahasiswa belum memahami penggunaan alat - alat di laboratorium patologi klinik di program studi Kedokteran Hewan Universitas Nusa Cendana. Jika keadaan ini dibiarkan, maka akan menganggu mutu lulusan yang dihasilkan oleh program studi Kedokteran Hewan Universitas Nusa Cendana. Tujuan pengabdian ini adalah optimalisasi petunjuk penggunaan alat-alat laboratorium di laboratorium patologi klinik program studi kedokteran hewan Universitas Nusa Cendana. Mitra pengabdian kepada masyarakat adalah mhasiswa di program studi Kedokteran Hewan Universitas Nusa Cendana. Kegiatan pengabdian dilaksanakan mulai tanggal 23 Juni 2022 – 25 Juli 2022 dan dilakukan pada laboratorium patolog kilinik Kedokteran Hewan Universitas Nusa Cendana. Kegiatan pengabdian ini menggunakan metode diskusi dan studi literasi. Hasil pengabdian yang telah dilakukan memiliki dampak pada mahasiswa di program studi Kedokteran Hewan Universitas Nusa Cendana. Dampakmya adalah mahasiswa dapat memecahkan masalah yang berkaitan dengan petunjuk penggunaan alat laboratorium. Khususnya pada alat-alat laboratorium patologi klinik yang dapat memudahkan mahasiswa dalam mengoperasikan alat dengan aman, baik dan benar.
Peningkatan produksi babi pasca kejadian African swine fever (ASF) menggunakan metode Inseminasi buatan (IB) pada peternak babi di Kelurahan Sikumana, Kota Kupang Yustinus Oswin Primajuni Wuhan; Dede Rival Novian; Jayusman A. Joesoef
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 4 No. 1 (2023): Jurnal Pengabdian kepada Masyarakat Nusantara (JPkMN)
Publisher : Cv. Utility Project Solution

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (572.635 KB)

Abstract

Peternakan babi menjadi salah satu sektor peternakan yang membawa dampak peningkatan ekonomi bagi masyarakat di Nusa Tenggara Timur. Babi yang diternakkan digunakan pemenuhan kebutuhan akan daging babi dan kebutuhan acara adat. Babi tidak lepas dari permasalahan kesehatan. Salah satunya yang penyakit yang menimbulkan masalah kesehatan pada babi yaitu penyakit African Swine Fever (ASF). Penyakit African Swin Fever (ASF) disebabkan oleh virus ASF, yang merupakan virus dari genus Asfivirus. Penyakit ini dapat mengakibatkan penurunan ekonomi yang signifikan karena menyebabkan kematian pada babi. Belum adanya vaksin dan pengobatan efektif berimplikasi pada tingginya tingkat morbiditas dan mortalitas. Pencgahan yang dapat dilakukan adalah meningkatkan biosecurity di kandang peternak. Salah satu metode yang dapat dilakukan dengan tujuan untuk meningkatkan kembali produksi babi di kota kupang adalah menggunakan metode inseminasi buatan (IB) menggunakan semen segar. Pengabdian yang dilakukan di salah satu peternak babi di kelurahan Sikumana ini merupakan usaha meningkatkan kembali produksi babi di kendang peternak yang pernah mengalami kejadian ASF. Babi betina yang dilakukan inseminasi buatan dalam pengabdian ini menghasilkan anak babi sebanyak 9 ekor.
Gejala Klinis Ehrlichiosis Pada Pasien Anjing Di Yogyakarta Yustinus Oswin Primajuni Wuhan; Ida Tjahajati; Aris Haryanto
Jurnal Veteriner Nusantara Vol 5 No 2 (2022): Agustus 2022
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v5i2.9553

Abstract

Canine monocytic ehrlichiosis is a potentially fatal disease in dogs and requires an early diagnosis to start therapy properly so that it produces a good prognosis. The research was conducted to determine ehrlichiosis in Yogyakarta based on clinical, hematological, and molecular diagnoses using the gltA gene. A total of 51 dog patients who were suspected of ehrlichiosis were performed anamnesis, clinical examination, then blood samples were taken for molecular examination with PCR. The molecular examination results of dog patients who were positive for ehrlichiosis based on PCR were 33.3% (17/51), with clinical symptoms which were 58.8% weak (10/17), 47% anorexia (8/17), 47% (8/17) gastrointestinal disorders (diarrhea and vomiting), 41.1% (7/17) pale mucosa, 35.2% (6/17) tick infestation, 35.2% (6/17) fever, 29,4% (5/17) dehydration, 11.7% (2/17) bleeding. The conclusion of this study is that the clinical of patients with erhlichiosis are very varied.
SOSIALISASI TANGGAP RABIES MELALUI PENDEKATAN MASYARAKAT DI DESA KUALIN, KABUPATEN TIMUR TENGAH SELATAN Pandarangga, Putri; Utami, Tri; Foeh, Nancy Diana Frederika Katerina; Sitompul, Yeremia Yobelanno; Wuhan, Yustinus Oswin Primajuni; Simarmata, Yohanes Timbun Raja Mangiut Ronael; Joesoef, Jayusman Arsiyanti; Datta, Frans Umbu; Amalo, Maria; Dangur, Fransiska Aping
Jurnal Media Tropika Vol 4 No 2 (2024): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v4i2.19291

Abstract

Rabies is a zoonotic disease that can attack humans through dog bites. Various methods have been performed for prevention, including socialization activities about rabies. The aim of community service, conducted by the Clinical, Reproduction, Pathology, and Reproduction Laboratory, Faculty of Medicine and Veterinary Medicine, Nusa Cendana University in Kualin Village, was to increase knowledge about rabies. Socialization was a method to increase awareness about rabies in the Kualin community, which this method using the Asset Based Community Development (ABCD) approach. The ABCD concept consists of 1). Identification of community assets; 2). Partner involvement; 3). Participation of the community service team; and 4). Partnership collaboration. The poster was one of the communication, information, and education (KIE) tools for transferring knowledge. Based on the results of the pre-and post-test, it shows that this method is quite successful where public knowledge about the symptoms of animals infected with rabies (58.82% to 88.23%), symptoms of humans infected with rabies (35.29% to 76.47%), and assistance if bitten by infected dogs (76.47% to 88.23%) increased significantly. So, this method can be applied to socialize other animal diseases that cause economic losses to the Kualin community.
Laporan Kasus: Profil Hematologi Babi Landrace Usia 2 Bulan yang Mengalami Diare Banggur, Mikael; Wuhan, Yustinus Oswin Primajuni
Jurnal Veteriner Nusantara Vol 8 No 1 (2025): Februari, 2025
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v8i1.22025

Abstract

Pigs are among the livestock commodities widely developed by farmers across various regions in Indonesia as sources of animal-based food. The overall health of livestock significantly influences productivity and the sustainability of farming operations. Hematological parameters serve as crucial indicators of physiological conditions and the health status of animals. Hematological examinations facilitate early detection of health issues, enabling prompt preventive or therapeutic interventions. In this case, a two-month-old female Landrace piglet exhibited signs of illness over a three-week period. The observed symptoms included diarrhea, reduced appetite and water intake, accompanied by weight loss during the illness. Hematological analysis revealed that the piglet was experiencing microcytic anemia due to iron deficiency, along with a decrease in white blood cell count attributed to intrinsic factors
PROGRAM PELATIHAN INVESTIGASI PENYAKIT HEWAN MENULAR BAGI MAHASISWA PENDIDIKAN PROFESI DOKTER HEWAN DI KUPANG Gelolodo, Maria Aega; Simarmata, Yohanes Timbun Raja Mangiut Ronael; Kallau, Novalino Harold Geoffrey; Tabali, Zulkifli; Sitompul, Yeremia Yobelanno; Loe, Fhady Risckhy; Wuhan, Yustinus Oswin Primajuni
Jurnal Media Tropika Vol 5 No 1 (2025): Jurnal Media Tropika
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/mediatropika.v5i1.20572

Abstract

Infectious animal diseases, particularly zoonoses, pose a significant problem to the inhabitants of East Nusa Tenggara (NTT) Province because of the high animal population, especially livestock, and the close interaction between humans, animals, and the environment. The Faculty of Medicine and Veterinary Medicine, Universitas Nusa Cendana, Kupang, held the Animal Disease Investigation Training Program for Veterinary Professional Education Students (PPDH) to overcome this significant challenge. This one-day workshop had been attended by 80 participants, consisting of 26 men (32.5%) and 54 women (67.5%). This training aims to improve students' understanding and skills in zoonotic disease investigation through theoretical and practical approaches. Theoretical sessions were conducted by giving presentations to provide a foundation of knowledge. At the same time, interactive approaches in the form of group discussions and field practice were designed to improve the ability to analyze and handle cases in the field. The results of this training are expected to equip participants with the ability to investigate, identify risks, and implement disease prevention and control measures. The expected implication of this activity is to improve the ability of prospective veterinarians to face the challenges of animal diseases in NTT, especially in infectious disease detection. This training provides relevant theoretical and practical experience in protecting animal and public health in the region.
Infection with Kapsulotaenia sandgroundi (Carter, 1943) in Komodo Dragons (Varanus komodoensis Ouwens, 1912) on Rinca Island, East Nusa Tenggara, Indonesia Winarso, Aji; Maha, Inggrid Trinidad; Wuhan, Yustinus Oswin Primajuni; Nurcahyo, Raden Wisnu; Priyowidodo, Dwi; Foitová, Ivona
ARSHI Veterinary Letters Vol. 8 No. 4 (2024): ARSHI Veterinary Letters - November 2024
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.8.4.81-82

Abstract

Artikel ini menguraikan infeksi Kapsulotaenia sangroundi (Carter, 1943) pada komodo (Varanus komodoensis Ouwens 1912) di Loh Buaya di Pulau Rinca, Taman Nasional Komodo. Sampel feses komodo yang baru dijatuhkan dikumpulkan dan diawetkan dalam larutan SAF. Pemeriksaan parasit asli dilakukan di bawah mikroskop (perbesaran objektif 40x). Pengamatan lingkungan dilakukan untuk memperoleh informasi tambahan tentang siklus hidup dan penularan parasit. Pengamatan sampel feses mengungkapkan adanya telur cestode dalam kemasan telur (kapsul). Satu dari lima sampel menunjukkan hasil positif (20%). Siklus hidup Kapsulotaenia rumit dan menyoroti pentingnya memahami hubungan parasit dalam ekosistem. Memahami infeksi ini sangat penting untuk konservasi dan pengelolaan komodo, karena hal ini menyoroti perlunya pemantauan masalah kesehatan di habitat alami mereka.
Applying the One Health Approach to Rabies Control in the Timor Archipelago, East Nusa Tenggara: A Literature Review on Epidemiology and Strategic Interventions Kale, Maria Laurenci Fanny Permata; Riwu, Audrey Gracelia; Simarmata, Yohanes TRMR; Wuhan, Yustinus Oswin Primajuni; Loe, Fhady Rischky
Cendana Medical Journal Vol 13 No 1 (2025): Cendana Medical Journal
Publisher : Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/cmj.v13i1.23786

Abstract

Background: Rabies remains a fatal zoonotic threat in Indonesia, particularly in East Nusa Tenggara (NTT), including Timor Island. Despite national efforts, the disease persists with rising incidence, especially in areas with high dog populations, limited vaccination coverage, and weak cross-sectoral coordination. Objective: This review aims to assess the recent epidemiological trends of rabies in Timor Island and evaluate the effectiveness of One Health implementation in controlling the outbreak. Methods: A literature review was conducted using data from 2010–2025 from sources such as WHO, FAO, Indonesian government agencies, and peer-reviewed journals. Keywords included “rabies,” “One health,” “NTT,” “Timor,” and “epidemiology.” Data were analyzed descriptively and thematically to identify gaps, risks, and strategic responses. Results: Rabies remains endemic in Timor, with more than 50,000 dog bite cases and over 80 human deaths recorded between 2023–2024. Vaccination coverage in dogs remains as low as 17.6%, and there are only 324 vaccinators for the entire province. Social and cultural factors, such as free-roaming dogs and limited public awareness, contribute significantly to transmission. Implementation of One Health remains fragmented, with low community involvement and weak surveillance integration across sectors. Conclusion: A locally adapted One health approach is urgently needed to eliminate rabies in Timor. Strengthening dog vaccination, integrating digital case reporting, engaging traditional leaders, and enforcing village-based regulations are key to effective rabies control. Keywords: Rabies, Timor Island, One health, Dog Vaccination, Community Engagement, Indonesia
Gambaran Histologi Kulit Babi Hutan (Sus scrofa) Asal Pulau Timor Maha, Inggrid Trinidad; Amalo, Filphin Adolfin; Nitbani, Heny; Selan, Yulfia Nellymalik; Wuhan, Yustinus Oswin Primajuni; Wada, Flaviana Donakrist Peni
Bioma : Berkala Ilmiah Biologi Volume 27 Issue 2 Year 2025
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2025.65304

Abstract

Tujuan penelitian ini adalah untuk mengetahui struktur histologi kulit babi hutan. Penelitian ini menggunakan tiga sampel babi hutan yang berasal dari pulau timor dengan umur lima sampai enam bulan yang diambil pada regio lumbo dorsalis, abdominal ventralis, dan dorsal interscapularis pada masing-masing babi. Pengambilan sampel, pembuatan preparat, dan pewarnaan Hematoxylin Eosin (HE) dilakukan di laboratorium Anatomi, Fisiologi, Farmakologi, dan Biokimia (AFFB) Kedokteran Hewan, Universitas Nusa Cendana. Hasil yang diperoleh adalah kulit babi hutan tersusun atas tiga lapisan yaitu lapisan epidermis, lapisan dermis, dan lapisan hipodermis. Lapisan epidermis babi hutan tersusun atas empat lapisan yaitu stratum basale, stratum spinosum, stratum granulosum, dan stratum corneum. Lapisan dermis tersusun atas papilla dermal dan reticula dermis yang terdiri dari jaringan ikat padat yang mengandung serat kolagen dan serat elastin. Pada penelitian ini ditemukan folikel rambut primer, kelenjar sebacea, otot arrector pili, dan pembuluh darah yang terdapat pada lapisan dermis. Hipodermis tersusun atas jaringan ikat longgar yang mengandung banyak sel lemak serta ditemukan folikel rambut sekunder dan kelenjar sudorifera.
Studi In Silico: Potensi Senyawa Spesifik Kulit Batang Faloak (Sterculia quadrifida R. Br) sebagai Anti-Helmintik Riwu, Audrey Gracelia; Riwu, Katty Hendriana Priscilia; Pua Upa, Nurul Fatmawati; Kale, Maria Laurenci Fanny Permata; Jannah, Insani Fitrahulil; Wuhan, Yustinus Oswin Primajuni
Jurnal Veteriner Nusantara Vol 8 No 2 (2025): Agustus, 2025
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v8i2.25038

Abstract

Helminthiasis remains a significant global public health issue, particularly in tropical and subtropical regions. The extensive and repeated use of synthetic anthelmintic agents, including albendazole and mebendazole, has contributed to the emergence of drug resistance among various parasitic species. Therefore, the exploration of plant-derived compounds with potential anthelmintic activity is urgently needed. This study investigated the potential of bioactive compounds from the stem bark of Faloak (Sterculia quadrifida R. Br) as inhibitors of the Mitochondrial Rhodoquinol-Fumarate Reductase (MRFR) protein through an in silico approach. Five compounds: epicatechin, d-catechin, catechin-(4α→8)-catechin, spinasterol, and spinasterone were analyzed, with Atpeni as the control. Drug-likeness evaluation was conducted using Lipinski’s Rule of Five, while molecular docking, visualization and molecular interaction analysis was performed using PyRx, PyMOL, and Discovery Studio. Four compounds satisfied Lipinski’s criteria, indicating favorable pharmacokinetic properties. Among them, spinasterol and spinasterone demonstrated the strongest binding affinities to MRFR (–10.1 and –9.2 kcal/mol, respectively), exceeding that of the control ligand (–6.4 kcal/mol). Hydrophobic interactions involving key residues such as Leu146, Ala149, and Val153 contributed to complex stability. These results suggest that Faloak stem bark contains promising bioactive compounds that may serve as natural anti-helmintic candidates, warranting further in vitro and in vivo validation
Co-Authors Adeventino, Raul Aji Winarso, Aji Amalo, Maria Amalo, Maria Elizabeth Aping Dangur, Fransiska Aris Haryanto Aris Haryanto Arjuna Purba Astika Putri, Nur Layla Audrey Gracelia Riwu Banggur, Mikael Christvando Rendi Leba Tagu Bore Dangur, Fransiska Aping Deran Ola, Elisa Albertine Rahmita Dwi Priyowidodo Elisabet Tangkonda Fhady Risckhy Loe Filphin Adolfin Amalo Foeh, Nancy Diana Frederika Katerina Foitová, Ivona Frans Umbu Datta Gaina, Cynthia Dewi Gelolodo, Maria A.E.G.A Heny Nitbani Hoban, Michaelis Conchita Fatima I Gede Semarabawa Ida Tjahajati Ida Tjahajati Jannah, Insani Fitrahulil Jayusman A. Joesoef Jayusman Arsiyanti Joesoef Joesoef, Jayusman Arsiyanti Kale, Maria Laurenci Fanny Permata Kallau, Novalino Harold Geoffrey Korbinianus Feribertus Rinca Loe, Fhady Rischky Loe, Fhady Risckhy Luji, Jovan Adiarsa Maha, Inggrid Trinidad Malelak, Marlin Cindy Claudya Maria Aega Gelolodo Maria Laurenci Fanny Permata Kale Nemay Anggadewi Ndaong Nitbani, Heny Novian, Dede Rival Nurcahyo, Raden Wisnu Olivia Claudya Lalo Prakameng, Dandy Pua Upa, Nurul Fatmawati Putri, Mariana Febrilianti Resilinda Repi, Ermelinda Ue Riwu, Katty Hendriana Priscilia Saputra, Agus Selan, Yulfia Nellymalik Simarmata, Yohanes T.R.M.R. Simarmata, Yohanes Timbun Raja Mangihut Ronael Simarmata, Yohanes Timbun Raja Mangiut Ronael Simarmata, Yohanes TRMR Sitompul, Yeremia Yobelanno Tabali, Zulkifli Tarsisius Considus Tophianong Tri Utami TRMR Simarmata, Yohanes Wada, Flaviana Donakrist Peni Yohanes Timbun Raja Mangihut Ronael Simarmata Yohanes Timbun Raja Mangihut Ronael Simarmata