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All Journal Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi Journal of Health Sciences and Medical Development Journal of Vocational Health Science
Arafah Nurfadillah
Universitas Megarezky
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Transportation Veterinary

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Tem Gene Detection as ESBL Marker in Clinical Isolates Klebsiella pneumoniae : A Case Study Arafah Nurfadillah; Wahdaniar; Miladiarsi; Ade Irma; Tenri Ayu Adri
Journal of Health Sciences and Medical Development Vol. 1 No. 02 (2022): Journal of Health Sciences and Medical Development
Publisher : The Indonesian Institute of Science and Technology Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.683 KB) | DOI: 10.56741/hesmed.v1i02.155

Abstract

Klebsiella pneumoniae is one of the most common ESBL-producing microorganisms. The research aims to detect the Tem gene in clinical isolates of K. pneumonaie from the Laboratory of clinical pathology of Wahidin Sudirohusodo General Hospital. Methods: This research is a descriptive cross-sectional design. Fifty samples of K. pneumonieae were obtained from the Laboratory of clinical pathology from March to May 2018. Tem gene was detected using primary Tem 164.SE and Tem 165.AS (445 bp) of Polymerase Chain Reaction (PCR). The research results indicate that 48 isolates are positive, containing the Tem gene with the highest number found in the sputum specimen. Genotype detection of bacteria-producing ESBL is essential so that antibiotic therapy given to the patients is more effective and efficient.
Pengenalan Bioinformatika, Aplikasi dan Database pada Siswa-Siswi SMK Laniang Makassar Arafah Nurfadillah; Nur Insani Amir; Budyanita Asrun; Laila Syakirah Firani; Julianti Bunga
ABDIKAN: Jurnal Pengabdian Masyarakat Bidang Sains dan Teknologi Vol. 2 No. 1 (2023): Februari 2023
Publisher : Yayasan Literasi Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55123/abdikan.v2i1.1702

Abstract

Bioinformatics is one of the fields of science that is currently developing rapidly in society. This is due to the increase in molecular biology research, which has resulted in an unprecedented amount of biological data/information explosion. In its application, bioinformatics uses online software and databases. Community service activities have provided education about Introduction to Bioinformatics, Applications, and Databases to students of SMK Laniang Makassar, Tamalanrea District, Makassar City. This activity was attended by 23 participants from the Computer and Network Engineering (TKJ) department and nursing assistants. This activity aimed to provide knowledge about bioinformatics, databases, and the use of bioinformatics-based applications in various aspects. The activity was carried out for one day, on Wednesday, January 11, 2023, from 09:00 to 13:00 local time. The method used in this activity is by presenting bioinformatics introduction material, databases, and applications related to bioinformatics as well as direct practice using mobile phones, closed with a discussion/question and answer between participants and speakers at the end of the activity. It is known that the result of this service community activity was an increase in student knowledge related to bioinformatics, databases, and bioinformatics applications.
Karakterisasi Mikrobiota Mulut Penghasil Senyawa Antimikroba: Laboratory Research Ade Irma; Juniati Binti Lukman; Arafah Nurfadillah
Journal of Vocational Health Science Vol. 1 No. 1 (2022): Journal of Vocational Health Science (JOVAS)
Publisher : Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31884/jovas.v1i1.10

Abstract

Microbes or microorganisms are organisms that have a size so small that they can only be seen with the help of a microscope. Microbes are widespread in nature and can be found anywhere. Mikroba can be settled in the soil, water, air, there is even one that is found in living beings (on living tissues). The microbiome is a community of microbes or microorganisms in this case bacteria, archae, fungi, viruses that live above or within other living organisms. The human-associated microbiome is called the microbiota. The presence of microbes in the body can cause interactions that are beneficial, detrimental or some are neutral in nature. The use of antibiotics whose nature is synthetic can cause a negative effect for health besides that it can also cause  resistance properties.  Therefore, it is necessary to have a type of antibiotic that comes from living beings.  The sample used in this study was a bacterial isolate in the oral cavity (swab results) the next stage was characterization through biochemical tests.  The results of characterization through biochemical tests showed that isolates of oral microbiota bacteria showed the species Streptococcus sp. which has the ability to produce antimicrobial compounds.
Potensi Mikrobiota Mulut Genus Streptococcus Penghasil Senyawa Antimikroba : Laboratory Research Ade Irma; Juniati Binti Lukman; Arafah Nurfadillah
Journal of Vocational Health Science Vol. 2 No. 1 (2023): Journal of Vocational Health Science (JOVAS)
Publisher : Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31884/jovas.v2i1.11

Abstract

Mikrobioma yang berasosiasi dengan manusia disebut mikrobiota. Berbagai jenis mikrobiota mulut dapat menguntungkan dan membantu sistem pencernaan serta membantu sistem imun tubuh karena menghasilkan berbagai senyawa kimia yang bermanfaat melawan kuman patogen. mikrobiota mulut yang berperan sebagai bakteri probiotik, salah satunya yang tergolong dalam genus Streptococcus. Tujuan penelitian ini untuk mengetahui potensi microbiota mulut khususnya genus Streptococcus penghasil senyawa antimikroba. Metode penelitian yang digunakan adalah kuantitatif eksperimental berupa aktivitas antibakteri diukur zona hambat yang terbentuk. Hasil uji menunjukkan bahwa dari 40 swab diperoleh 17 isolat yang dapat menghambat pertumbuhan bakteri uji Staphylococcus aureus dan Escherichia coli dengan ukuran zona hambat yang paling besar yaitu 9,35 mm terhadap S. aureus dan terkecil 7,00 mm. sementara pada bakteri uji E.coli terdapat zona hambat yang dihasilkan sekitar 9,00 mm dan hasil pengukuran yang paling kecil adalah sekitar 7,00 mm. dari hasil pengujian memperlihatkan bahwa microbiota mulut genus Streptococcus berpotensi menghasilkan senyawa antimikroba.
Co-Authors Ade Irma Ade Irma Budyanita Asrun Julianti Bunga Juniati Binti Lukman Laila Syakirah Firani Miladiarsi Nur Insani Amir Tenri Ayu Adri