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Exploring the Streptococci Variants in Children’s Oral Cavity, Its Microbiome Diversity Juniati Binti Lukman; Zaraswati Dwyana; Mochammad Hatta; Cyril B. Romero
Journal of Health Sciences and Medical Development Vol. 2 No. 02 (2023): Journal of Health Sciences and Medical Development
Publisher : The Indonesian Institute of Science and Technology Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56741/hesmed.v2i02.264

Abstract

This study aimed to identify the variants of the Streptococcus genus in the oral cavity of healthy individuals. Streptococcus is one of the normal flora, which is part of the lactic acid bacteria (LAB) in the oral cavity. LAB has the ability to produce antimicrobial agents, regulate the host's immune response, and inhibit the growth of pathogenic bacteria. The presence of LAB in the oral cavity is crucial in maintaining oral health and preventing periodontal diseases. This study used observational cross-sectional design with 40 healthy elementary school children as subjects. Swabs were taken from their oral cavities and sent to the laboratory for PCR and sequencing tests. The results revealed that isolates 3S, 4S, 9S, 10S, 14S, and 15S were identified as Streptococcus dysgalactiae, while isolates 1S, 8S, and 9S were identified as Streptococcus mitis. Additionally, isolates 2S, 5S, 7S, 11S, 12S, and 13S were identified as Streptococcus salivarius, isolate 6S was identified as Streptococcus agalactiae, and isolate 17S was identified as Streptococcus oralis. These findings provide valuable information on the identification of oral microbiota, particularly Streptococcus genus variants, which can potentially serve as an alternative therapy for periodontal diseases.
Karakterisasi Mikrobiota Mulut Penghasil Senyawa Antimikroba: Laboratory Research Ade Irma; Juniati Binti Lukman; Arafah Nurfadillah
Journal of Vocational Health Science Vol. 1 No. 1 (2022): Journal of Vocational Health Science (JOVAS)
Publisher : Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31884/jovas.v1i1.10

Abstract

Microbes or microorganisms are organisms that have a size so small that they can only be seen with the help of a microscope. Microbes are widespread in nature and can be found anywhere. Mikroba can be settled in the soil, water, air, there is even one that is found in living beings (on living tissues). The microbiome is a community of microbes or microorganisms in this case bacteria, archae, fungi, viruses that live above or within other living organisms. The human-associated microbiome is called the microbiota. The presence of microbes in the body can cause interactions that are beneficial, detrimental or some are neutral in nature. The use of antibiotics whose nature is synthetic can cause a negative effect for health besides that it can also cause  resistance properties.  Therefore, it is necessary to have a type of antibiotic that comes from living beings.  The sample used in this study was a bacterial isolate in the oral cavity (swab results) the next stage was characterization through biochemical tests.  The results of characterization through biochemical tests showed that isolates of oral microbiota bacteria showed the species Streptococcus sp. which has the ability to produce antimicrobial compounds.
Potensi Mikrobiota Mulut Genus Streptococcus Penghasil Senyawa Antimikroba : Laboratory Research Ade Irma; Juniati Binti Lukman; Arafah Nurfadillah
Journal of Vocational Health Science Vol. 2 No. 1 (2023): Journal of Vocational Health Science (JOVAS)
Publisher : Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31884/jovas.v2i1.11

Abstract

Mikrobioma yang berasosiasi dengan manusia disebut mikrobiota. Berbagai jenis mikrobiota mulut dapat menguntungkan dan membantu sistem pencernaan serta membantu sistem imun tubuh karena menghasilkan berbagai senyawa kimia yang bermanfaat melawan kuman patogen. mikrobiota mulut yang berperan sebagai bakteri probiotik, salah satunya yang tergolong dalam genus Streptococcus. Tujuan penelitian ini untuk mengetahui potensi microbiota mulut khususnya genus Streptococcus penghasil senyawa antimikroba. Metode penelitian yang digunakan adalah kuantitatif eksperimental berupa aktivitas antibakteri diukur zona hambat yang terbentuk. Hasil uji menunjukkan bahwa dari 40 swab diperoleh 17 isolat yang dapat menghambat pertumbuhan bakteri uji Staphylococcus aureus dan Escherichia coli dengan ukuran zona hambat yang paling besar yaitu 9,35 mm terhadap S. aureus dan terkecil 7,00 mm. sementara pada bakteri uji E.coli terdapat zona hambat yang dihasilkan sekitar 9,00 mm dan hasil pengukuran yang paling kecil adalah sekitar 7,00 mm. dari hasil pengujian memperlihatkan bahwa microbiota mulut genus Streptococcus berpotensi menghasilkan senyawa antimikroba.